FastQCFastQC Report
Wed 25 May 2016
SRR2431461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6678745
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT686991.0286213951872694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT568370.8510131768767936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432690.6478612374031348No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161350.24158730420161273No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130570.19550080142302184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124790.18684648088825073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118580.17754832681888588No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97880.14655447992100312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA245750.059.9470561
GTATCAA461350.043.4773561
GTGGTAT86700.033.91871
TGGTATC88500.032.8428842
TATCAAC627800.031.8917522
ATCAACG632850.031.6298313
TCAACGC643000.031.1524854
CAACGCA677450.029.5816695
AACGCAG698450.028.7715136
TATAACG6700.028.0789762
ACGCAGA769500.025.9744577
CGCAGAG769450.025.866188
CATGGGT194050.025.2066884
GCAGAGT799250.024.601819
TACCTGG50150.024.477422
GTACATG426300.024.2379191
TACATGG425650.023.723322
ACATGGG434000.022.638353
ATAACGC8450.022.2638033
AGTACTT558250.022.11106912-13
Produced by FastQC (version 0.11.5)