FastQCFastQC Report
Wed 25 May 2016
SRR2431460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4528867
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA245950.5430718102342154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55900.039.777951
TACCTGG45700.034.870052
GTATCAA91700.034.1427081
GTACCTG52850.030.421051
TCAACGC101200.030.1459164
ATCAACG102100.030.0183013
TCTGTCG13100.029.4197968
TATCAAC111700.027.5649492
CAACGCA113050.027.0685585
AACGCAG119550.025.5946
GTCTCGC13650.025.141041
CGCAGAG122700.023.9787718
GTACATG164950.023.9397741
CTGTCGC16500.023.9272359
ACGCAGA127700.023.4084667
TACATGG165350.023.0845222
CATGGGT79000.023.027574
ACCTGGG68250.022.6598743
ACATGGG172250.021.1771133
TCGCTCT16450.021.1460914
Produced by FastQC (version 0.11.5)