##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431460_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4528867
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.80203061825397	31.0	30.0	34.0	25.0	34.0
2	29.79299149213258	31.0	30.0	34.0	25.0	34.0
3	29.88680965901626	31.0	30.0	34.0	25.0	34.0
4	33.22770463341052	37.0	35.0	37.0	26.0	37.0
5	33.37199767623999	37.0	35.0	37.0	27.0	37.0
6	33.49658424502199	37.0	35.0	37.0	28.0	37.0
7	33.38565274714404	37.0	35.0	37.0	27.0	37.0
8	33.30771713984977	37.0	35.0	37.0	27.0	37.0
9	34.63756475957452	39.0	35.0	39.0	25.0	39.0
10-11	34.573834581585196	39.0	35.0	39.0	25.5	39.0
12-13	34.53564544951309	39.0	35.0	39.0	25.5	39.0
14-15	35.683696275470226	40.0	36.0	41.0	25.5	41.0
16-17	35.6760343149843	40.0	36.0	41.0	25.5	41.0
18-19	35.654669810352125	40.0	36.0	41.0	25.0	41.0
20-21	35.75739075578947	40.0	36.0	41.0	26.0	41.0
22-23	35.711869547063316	40.0	36.0	41.0	26.0	41.0
24-25	35.75225172211947	40.0	36.0	41.0	26.0	41.0
26-27	35.7480663486033	40.0	36.0	41.0	27.0	41.0
28-29	35.64590084009974	40.0	35.0	41.0	26.5	41.0
30-31	35.61965023481591	40.0	35.0	41.0	27.0	41.0
32-33	35.55809587695995	40.0	35.0	41.0	26.0	41.0
34-35	35.46002377636614	40.0	35.0	41.0	25.5	41.0
36-37	35.327880902662855	40.0	35.0	41.0	25.0	41.0
38-39	35.21449492776008	40.0	35.0	41.0	25.0	41.0
40-41	35.12273522273893	40.0	35.0	41.0	25.0	41.0
42-43	34.90257463952905	39.0	34.5	41.0	24.0	41.0
44-45	34.765977009260816	39.0	34.0	41.0	24.0	41.0
46-47	34.62582010025908	39.0	34.0	41.0	23.5	41.0
48-49	34.488417632931146	39.0	34.0	41.0	23.0	41.0
50-51	34.32449042994639	39.0	33.0	40.0	23.0	41.0
52-53	34.227644684641874	39.0	33.0	40.0	22.0	41.0
54-55	34.158334413441594	39.0	33.0	40.0	22.0	41.0
56-57	34.00726903660452	38.0	33.0	40.0	20.5	41.0
58-59	33.80110820211766	38.0	33.0	40.0	19.5	41.0
60-61	33.578676631484214	38.0	33.0	40.0	18.5	41.0
62-63	33.294957105165594	37.5	32.5	40.0	17.0	41.0
64-65	33.142252466234936	37.0	32.0	40.0	15.0	41.0
66-67	32.88771330666147	37.0	32.0	40.0	13.0	41.0
68-69	32.611187411774296	36.0	32.0	39.5	9.5	41.0
70-71	32.29797276007443	36.0	32.0	39.0	7.5	41.0
72-73	31.974573110669844	35.0	31.0	39.0	6.5	41.0
74-75	31.637971152608365	35.0	31.0	38.5	2.0	40.0
76-77	31.30656044878333	35.0	31.0	37.0	2.0	39.5
78-79	30.957364722788284	35.0	31.0	37.0	2.0	39.0
80-81	30.642044356789455	35.0	31.0	36.5	2.0	39.0
82-83	30.328527090771267	35.0	30.5	36.0	2.0	38.5
84-85	30.02584951158866	35.0	30.0	36.0	2.0	37.0
86-87	29.75038480926907	35.0	30.0	35.0	2.0	37.0
88-89	29.466644416804467	34.0	30.0	35.0	2.0	36.5
90-91	29.231047191273227	34.0	30.0	35.0	2.0	36.0
92-93	29.017339215304844	34.0	29.5	35.0	2.0	36.0
94-95	28.770518652899277	34.0	29.0	35.0	2.0	36.0
96-97	28.65784389340645	34.0	29.0	35.0	2.0	35.0
98-99	28.482479282345892	34.0	29.0	35.0	2.0	35.0
100	28.332698001508987	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.409623943620808
1101	2	0.43251213432367663
1101	3	0.4712553906318213
1101	4	0.47065415810493505
1101	5	0.408441862801034
1101	6	0.4738522718985294
1101	7	0.5535259595819966
1101	8	0.6402828501637856
1101	9	0.7627685432824478
1101	10-11	0.8163408586946446
1101	12-13	0.7502669796600117
1101	14-15	0.8487163038183567
1101	16-17	0.8369537767208257
1101	18-19	0.7916561542489191
1101	20-21	0.8458950038767838
1101	22-23	0.8416333341555884
1101	24-25	0.7856805022842437
1101	26-27	0.8774247658459942
1101	28-29	0.9546672971575916
1101	30-31	0.9416296340917611
1101	32-33	0.9259598183110072
1101	34-35	0.8932369589201556
1101	36-37	0.9180684273518906
1101	38-39	0.8784529166429778
1101	40-41	0.8518859246089505
1101	42-43	0.8640154725818903
1101	44-45	0.7542214037069712
1101	46-47	0.7088275972024505
1101	48-49	0.7427004900943501
1101	50-51	0.7750341275870056
1101	52-53	0.6959688535073099
1101	54-55	0.8014099904773317
1101	56-57	0.8097878110906649
1101	58-59	0.843730874527175
1101	60-61	0.8588647444934665
1101	62-63	0.8155042339796168
1101	64-65	0.8641308118623883
1101	66-67	0.8509938233737628
1101	68-69	0.8074229749476061
1101	70-71	0.8335933640252051
1101	72-73	0.8324090143771556
1101	74-75	0.8132886677480471
1101	76-77	0.7673365600441393
1101	78-79	0.7460248249028574
1101	80-81	0.7187666271162172
1101	82-83	0.7400199452684824
1101	84-85	0.7355461630547104
1101	86-87	0.6924892333821298
1101	88-89	0.7667710631202915
1101	90-91	0.7500801586899328
1101	92-93	0.7277309046924607
1101	94-95	0.7353269858111844
1101	96-97	0.7668317610666975
1101	98-99	0.7121799571378169
1101	100	0.760146368958651
1102	1	0.46201879388396705
1102	2	0.5228841713962176
1102	3	0.5258235774813826
1102	4	0.5103376608205181
1102	5	0.4655595082910722
1102	6	0.5426527099216827
1102	7	0.6628085713910039
1102	8	0.6612933695994201
1102	9	0.79078903380627
1102	10-11	0.7730433057252242
1102	12-13	0.7386506899986927
1102	14-15	0.8837430139863116
1102	16-17	0.8625155594461447
1102	18-19	0.7933734857590053
1102	20-21	0.842303450919232
1102	22-23	0.7955768555515448
1102	24-25	0.8503821352741952
1102	26-27	0.8942125802826482
1102	28-29	0.9740383104951604
1102	30-31	0.9602497106425858
1102	32-33	0.9383747335705479
1102	34-35	0.8850442825710729
1102	36-37	0.8365956909033159
1102	38-39	0.7446601087159834
1102	40-41	0.8468801454525519
1102	42-43	0.8591490652559557
1102	44-45	0.7590754653715663
1102	46-47	0.7657957874351311
1102	48-49	0.7373328784752999
1102	50-51	0.6705589231202254
1102	52-53	0.62828296951146
1102	54-55	0.7463054267046871
1102	56-57	0.797046474726713
1102	58-59	0.8351552098446149
1102	60-61	0.7998185725613283
1102	62-63	0.8420693119961271
1102	64-65	0.8528184232731704
1102	66-67	0.856051577082269
1102	68-69	0.9131104642432106
1102	70-71	0.8774283511428607
1102	72-73	0.8597351012392735
1102	74-75	0.8219918019522297
1102	76-77	0.7680499028142869
1102	78-79	0.7218997315686337
1102	80-81	0.7534505576101012
1102	82-83	0.7085602558875941
1102	84-85	0.7580920007559158
1102	86-87	0.708836184934821
1102	88-89	0.7539302793849707
1102	90-91	0.716793254592325
1102	92-93	0.7203266113625482
1102	94-95	0.67684296208596
1102	96-97	0.6993631879934661
1102	98-99	0.7330441929915139
1102	100	0.6311155872688907
1103	1	0.25051971917506677
1103	2	0.30414418342845195
1103	3	0.34561970118609864
1103	4	0.34724164885941633
1103	5	0.2944342362453085
1103	6	0.4477341875218457
1103	7	0.46378351236305093
1103	8	0.48875821831075683
1103	9	0.5348802834473787
1103	10-11	0.6385284856532962
1103	12-13	0.5392601377365622
1103	14-15	0.6585937167432476
1103	16-17	0.5901893404498537
1103	18-19	0.5917657452354845
1103	20-21	0.6326134641491308
1103	22-23	0.5422336865586104
1103	24-25	0.6655428016794644
1103	26-27	0.6130623858997879
1103	28-29	0.6646601542844621
1103	30-31	0.5783878401568074
1103	32-33	0.6618802588185488
1103	34-35	0.6426110072878402
1103	36-37	0.6373584999195714
1103	38-39	0.6556741082791788
1103	40-41	0.6129432546708387
1103	42-43	0.62416512207934
1103	44-45	0.5822786576837018
1103	46-47	0.5456837454467305
1103	48-49	0.5196237572771878
1103	50-51	0.4866613527203043
1103	52-53	0.48783170502409234
1103	54-55	0.6076647816754601
1103	56-57	0.5349281957304726
1103	58-59	0.5349505239375247
1103	60-61	0.6499993017597703
1103	62-63	0.5824600803677598
1103	64-65	0.5455715800595655
1103	66-67	0.5241666643922116
1103	68-69	0.6089718119850787
1103	70-71	0.5586285193323377
1103	72-73	0.5223301741586539
1103	74-75	0.4818661362729415
1103	76-77	0.526615673956421
1103	78-79	0.5049410328109296
1103	80-81	0.4644348875669735
1103	82-83	0.43457064161605174
1103	84-85	0.42117216865363005
1103	86-87	0.4168887130953287
1103	88-89	0.4376024960793998
1103	90-91	0.43612985197900755
1103	92-93	0.4126485958731365
1103	94-95	0.4465852655567133
1103	96-97	0.4943477067569262
1103	98-99	0.40558263869947453
1103	100	0.4412922490164064
1104	1	0.2812850516413512
1104	2	0.3431907478263163
1104	3	0.3062870040502368
1104	4	0.362873448829383
1104	5	0.32076405145839715
1104	6	0.37784365174016443
1104	7	0.436194619001256
1104	8	0.41838169102460654
1104	9	0.49975437274530776
1104	10-11	0.6092425999531343
1104	12-13	0.5629330183177501
1104	14-15	0.6714602263359666
1104	16-17	0.7072631305092685
1104	18-19	0.5877242352647656
1104	20-21	0.5334925197823424
1104	22-23	0.5500315254704731
1104	24-25	0.6273224057298421
1104	26-27	0.7108146024768089
1104	28-29	0.7492358930470502
1104	30-31	0.7027834545219065
1104	32-33	0.7514998596831575
1104	34-35	0.7107794962052125
1104	36-37	0.6862873371314322
1104	38-39	0.6829356567207441
1104	40-41	0.617161889339684
1104	42-43	0.651918757236551
1104	44-45	0.5915122075481634
1104	46-47	0.6174381326460718
1104	48-49	0.6298839887895653
1104	50-51	0.6492593982020907
1104	52-53	0.6593121627398091
1104	54-55	0.6422015420972329
1104	56-57	0.6935105343021775
1104	58-59	0.7052520715715218
1104	60-61	0.6836711624089631
1104	62-63	0.6533441355712029
1104	64-65	0.6774193172359517
1104	66-67	0.7043211743597908
1104	68-69	0.7357701372092507
1104	70-71	0.6084639924733111
1104	72-73	0.651865698321906
1104	74-75	0.6926664697943394
1104	76-77	0.6845363506215314
1104	78-79	0.7387117043186322
1104	80-81	0.6810992098942279
1104	82-83	0.6335463510018577
1104	84-85	0.6062119796858276
1104	86-87	0.6474749789506049
1104	88-89	0.6355493445119649
1104	90-91	0.6288889485523654
1104	92-93	0.6283697751643267
1104	94-95	0.627727017947894
1104	96-97	0.6825773235847912
1104	98-99	0.7083066163769693
1104	100	0.7139656914049723
1105	1	0.21368997212167784
1105	2	0.2177961581563217
1105	3	0.3026420246157002
1105	4	0.2202140445723657
1105	5	0.20588873223287152
1105	6	0.33603514342583196
1105	7	0.38321510504277256
1105	8	0.4292213101491882
1105	9	0.5287591757972621
1105	10-11	0.5639136387212744
1105	12-13	0.48141676225068863
1105	14-15	0.6095390466049864
1105	16-17	0.5136818163692354
1105	18-19	0.45818701040254695
1105	20-21	0.5035587788644023
1105	22-23	0.51799157837754
1105	24-25	0.4872398028958784
1105	26-27	0.512158196574525
1105	28-29	0.617964041345175
1105	30-31	0.537038492829609
1105	32-33	0.48734071506671484
1105	34-35	0.46725024108197033
1105	36-37	0.47095757309048736
1105	38-39	0.40393767639402256
1105	40-41	0.36667792615798334
1105	42-43	0.3879715202873726
1105	44-45	0.3606957787776608
1105	46-47	0.3326820968590951
1105	48-49	0.2650585440520814
1105	50-51	0.318018485962142
1105	52-53	0.280188066195862
1105	54-55	0.3039854379146121
1105	56-57	0.3432920363954537
1105	58-59	0.30589457855096924
1105	60-61	0.2859854726997142
1105	62-63	0.26839211215824577
1105	64-65	0.3250537396368074
1105	66-67	0.33266020719078426
1105	68-69	0.3166443868744082
1105	70-71	0.36758617509359226
1105	72-73	0.30549270409054685
1105	74-75	0.34042082135782437
1105	76-77	0.33333390368122906
1105	78-79	0.3678154374101865
1105	80-81	0.4097259575198535
1105	82-83	0.3415157506759705
1105	84-85	0.3355935207445704
1105	86-87	0.36253201201161644
1105	88-89	0.42222181731955644
1105	90-91	0.3989682079891601
1105	92-93	0.4019580203135291
1105	94-95	0.3784568401019115
1105	96-97	0.4300876676845107
1105	98-99	0.4883763248362136
1105	100	0.4802515260806324
1106	1	-0.010114372904137525
1106	2	-0.01449559412969137
1106	3	-0.008937534208008913
1106	4	-0.030937027003560047
1106	5	0.042517474897209695
1106	6	0.0993576974115129
1106	7	0.0666888438775004
1106	8	0.15201464151148514
1106	9	0.16807392252552944
1106	10-11	0.2082001550904664
1106	12-13	0.15833116989567486
1106	14-15	0.23491287855266307
1106	16-17	0.23702180296456987
1106	18-19	0.19782365519564138
1106	20-21	0.28609713530831016
1106	22-23	0.26981631828897434
1106	24-25	0.2620095558664346
1106	26-27	0.2783259197072212
1106	28-29	0.353239047612
1106	30-31	0.24591718304890264
1106	32-33	0.28583626817687247
1106	34-35	0.26307043248490913
1106	36-37	0.3264441424567792
1106	38-39	0.34052789802084504
1106	40-41	0.3696399772110368
1106	42-43	0.27577264991066386
1106	44-45	0.305061995255258
1106	46-47	0.2967491046536779
1106	48-49	0.24284708686502654
1106	50-51	0.20062872176242053
1106	52-53	0.2893851172509585
1106	54-55	0.29480411014924357
1106	56-57	0.28399590203473224
1106	58-59	0.2925577018546832
1106	60-61	0.2902789428931456
1106	62-63	0.2654818314167642
1106	64-65	0.2915537782805231
1106	66-67	0.29662143548027586
1106	68-69	0.33632906641985727
1106	70-71	0.2771134765722323
1106	72-73	0.2198267011143642
1106	74-75	0.2630394176953814
1106	76-77	0.23913888499934544
1106	78-79	0.2527496326824057
1106	80-81	0.25778825119001425
1106	82-83	0.22101587274104517
1106	84-85	0.26187174854784345
1106	86-87	0.3304353082592506
1106	88-89	0.3373121884096406
1106	90-91	0.2927356615563852
1106	92-93	0.2458985322998295
1106	94-95	0.3371517068669654
1106	96-97	0.3544459979815535
1106	98-99	0.3550521617180209
1106	100	0.3532121295278152
1107	1	0.12461371951626177
1107	2	0.16042378848199945
1107	3	0.23403701261269205
1107	4	0.2118950696746893
1107	5	0.17322878637775574
1107	6	0.22861633051630292
1107	7	0.27615629949880116
1107	8	0.273980140400063
1107	9	0.4280548248141258
1107	10-11	0.3758185765550337
1107	12-13	0.2970478663046805
1107	14-15	0.45536934516297123
1107	16-17	0.4086679630722685
1107	18-19	0.4295532503867818
1107	20-21	0.42574367110869105
1107	22-23	0.4403416618912388
1107	24-25	0.4619211115472197
1107	26-27	0.4718788767217177
1107	28-29	0.5673760502105409
1107	30-31	0.5253464432473862
1107	32-33	0.5785462647330277
1107	34-35	0.5452649473717344
1107	36-37	0.3915266383085054
1107	38-39	0.41133970729419644
1107	40-41	0.4647575377132114
1107	42-43	0.4433104539869319
1107	44-45	0.4074351207053013
1107	46-47	0.40090833573598417
1107	48-49	0.4572995147789882
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2216	4	-0.5280100727829478
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2216	90-91	-1.7778874979251214
2216	92-93	-1.6964366641265727
2216	94-95	-1.7276728749045773
2216	96-97	-1.863716671604145
2216	98-99	-2.1421916215218815
2216	100	-2.14535756415831
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	145333.0
3	26806.0
4	18292.0
5	16051.0
6	12354.0
7	12061.0
8	13608.0
9	14402.0
10	15320.0
11	15775.0
12	17024.0
13	18280.0
14	19159.0
15	19547.0
16	19235.0
17	18928.0
18	18267.0
19	17968.0
20	18022.0
21	18655.0
22	22615.0
23	31879.0
24	46562.0
25	57896.0
26	61260.0
27	54632.0
28	58183.0
29	69079.0
30	81215.0
31	94706.0
32	118150.0
33	153169.0
34	206541.0
35	304950.0
36	480259.0
37	812619.0
38	1012056.0
39	387141.0
40	868.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83635936425857	21.294325775193844	13.452232495104695	22.417082365442894
2	16.45092871582941	21.990851406571394	38.788644968108	22.769574909491197
3	17.88829482308901	27.07223508399706	30.279285024683716	24.76018506823022
4	12.453395657945991	19.34383824572468	38.39906301092535	29.803703085403978
5	12.216690070414401	38.476070431626354	34.11785021763388	15.189389280325372
6	28.250535835976997	39.441061134104075	18.700642473188328	13.6077605567306
7	25.300551395492114	34.17503770555878	22.538097519934812	17.98631337901429
8	23.913800508549617	36.0039217721287	21.49327656774276	18.58900115157892
9	24.197409496100022	18.695310290095186	21.99609726324836	35.11118295055643
10-11	22.242269984771333	28.653558433075617	28.920957212331018	20.18321436982204
12-13	23.271293803098427	26.878520113641734	28.235545590288254	21.61464049297159
14-15	21.326602299861776	29.06324978721238	25.819813356360438	23.7903345565654
16-17	20.36394201102593	31.847299720437828	26.218992540555615	21.569765727980627
18-19	20.139487777844145	30.37783635066639	28.419047614841585	21.063628256647885
20-21	22.202379506565944	29.928080429795063	27.34429755314474	20.52524251049425
22-23	21.568199455929314	30.187134568781637	27.411654206522734	20.833011768766315
24-25	21.261852473041873	29.82159006333795	27.775160486799194	21.141396976820985
26-27	21.081534891593343	29.486215390805075	27.930697202536003	21.501552515065573
28-29	21.073013625391074	29.658606649784453	27.94179059090768	21.326589133916798
30-31	21.65185489175	30.446950041850123	27.183578897115385	20.717616169284494
32-33	20.734605253845864	29.871086690366816	28.103887603925532	21.29042045186179
34-35	20.910488366421163	29.096516986905346	28.211303096189518	21.781691550483977
36-37	21.725434402584725	29.93039025598283	27.32880515399391	21.01537018743854
38-39	21.461905975867236	29.80127770785883	27.717370482835253	21.01944583343868
40-41	20.96677835902655	29.445876864886838	28.065137155928817	21.522207620157797
42-43	20.819193342334497	29.520624633101804	28.017248755678953	21.642933268884747
44-45	21.412116110666297	29.68191411513291	27.760386281845133	21.145583492355666
46-47	21.10580316015683	30.29315007874336	27.440492015697444	21.16055474540237
48-49	20.882254712268043	29.575328664997695	28.01753248007861	21.52488414265565
50-51	20.925210592554016	29.199190188728156	28.11155142541159	21.764047793306236
52-53	20.96042932860767	29.355690223705437	27.894005083747807	21.78987536393909
54-55	21.27123440245352	30.060600480007736	27.52898915069456	21.139175966844178
56-57	20.776710991500327	29.688901803870067	27.610210558600663	21.924176646028947
58-59	20.65526393973817	28.99826785216871	28.211199927220864	22.135268280872257
60-61	21.302511340912787	29.727654692170173	27.397766175815946	21.572067791101095
62-63	21.098806853430187	29.590805565213014	27.57195462446007	21.738432956896723
64-65	20.964940792928275	29.090296028734187	28.090192687408834	21.854570490928708
66-67	20.77734660674729	29.24781485053719	27.62129610983335	22.353542432882172
68-69	21.578905181647936	29.51846576073264	27.434648940443168	21.46798011717626
70-71	21.600687441994598	29.052252171883463	27.662982070023602	21.684078316098336
72-73	21.063737187743513	29.143118140934103	28.280322975183765	21.51282169613862
74-75	21.037003424770965	29.106103864846304	28.02312769250812	21.833765017874608
76-77	21.081966232137685	29.245710786816787	27.581794496898553	22.090528484146976
78-79	21.37490536813438	29.35079973241967	27.771660664068666	21.502634235377286
80-81	20.96452589610468	29.011438572379962	28.00751074477688	22.016524786738483
82-83	20.77368235515316	29.05637784057819	28.220992735508084	21.948947068760567
84-85	20.97284283795915	28.924684492042374	27.966918600498126	22.13555406950035
86-87	21.410120371362996	29.04201609419233	27.732324613502122	21.815538920942554
88-89	20.951191423696915	28.87661633439907	28.013176545819103	22.159015696084914
90-91	20.763796639451122	28.67631796968016	27.97532406049836	22.58456133037036
92-93	21.081896528405785	28.999687442692768	27.668244100301422	22.250171928600032
94-95	20.982388851811432	28.643886939332262	28.01400810257446	22.35971610628184
96-97	20.886050724177665	28.661044974895816	28.04321384358619	22.40969045734033
98-99	20.817210621426213	28.64033979802191	28.075356694300137	22.467092886251745
100	21.092014910905093	28.818843808666646	27.736981773843027	22.352159506585238
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18596.0
1	13760.0
2	7976.0
3	5897.0
4	3985.5
5	2889.0
6	2528.5
7	2636.5
8	2998.0
9	3054.0
10	2557.5
11	2037.0
12	1882.5
13	1871.5
14	1911.0
15	2099.5
16	2378.5
17	2820.5
18	3498.5
19	4283.5
20	5385.0
21	7117.0
22	9869.0
23	14304.5
24	19856.0
25	26463.0
26	35631.0
27	47329.0
28	62682.0
29	77834.0
30	89084.0
31	100753.0
32	112350.0
33	122514.5
34	131553.0
35	139439.0
36	145485.0
37	149756.5
38	152546.0
39	152873.5
40	152595.5
41	150922.0
42	146036.5
43	141897.5
44	139406.5
45	137041.5
46	136751.5
47	136197.5
48	136029.5
49	137661.0
50	137600.0
51	133265.5
52	127287.5
53	121287.5
54	116189.0
55	112551.5
56	104542.0
57	94801.5
58	87217.5
59	79082.5
60	68309.5
61	56301.5
62	45886.5
63	36403.0
64	27959.0
65	21098.5
66	15745.0
67	11701.5
68	8584.5
69	6293.0
70	4557.5
71	3401.0
72	2633.0
73	1979.5
74	1450.0
75	1093.0
76	835.0
77	654.0
78	503.0
79	396.0
80	310.0
81	206.5
82	161.5
83	137.0
84	112.5
85	93.5
86	74.0
87	64.0
88	59.0
89	49.5
90	33.5
91	26.5
92	26.5
93	23.0
94	17.5
95	14.0
96	13.0
97	10.5
98	13.5
99	21.0
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012100156617538117
2	0.006668334486307503
3	0.008280216663461303
4	0.007043704308384416
5	0.008611425329999756
6	0.00947256786299973
7	0.00788276626361516
8	0.006668334486307503
9	0.005520144442307535
10-11	0.005244137220192158
12-13	0.005498063864538305
14-15	0.006414407841961356
16-17	0.00394138313180758
18-19	0.005001250864730627
20-21	0.004349873820538338
22-23	0.005023331442499857
24-25	0.0049350091314229365
26-27	0.00546494299788446
28-29	0.013910763994614989
30-31	0.004559639309346024
32-33	0.004493397576038334
34-35	0.004140108331730652
36-37	0.005796151664422912
38-39	0.004647961620422945
40-41	0.004581719887115254
42-43	0.004250511220576802
44-45	0.003378328398692212
46-47	0.005100613464692162
48-49	0.002859434821115303
50-51	0.0034776909986537472
52-53	0.002682790198961462
54-55	0.004195309776153727
56-57	0.002296380087999935
58-59	0.0021307757547307086
60-61	0.0028925556877691483
62-63	0.002815273665576843
64-65	0.0040849068873075765
66-67	0.002539266443461466
68-69	0.0029587974210768388
70-71	0.004283632087230648
72-73	0.002163896621384554
74-75	0.002141816043615324
76-77	0.002064534021423018
78-79	0.002947757132192224
80-81	0.0034224895542306717
82-83	0.001269633221730733
84-85	0.002174936910269169
86-87	0.0019982922881153276
88-89	0.0013800361105768837
90-91	0.0018326879548461019
92-93	0.002152856332499939
94-95	0.0013137943772691934
96-97	8.942633996538206E-4
98-99	0.0013027540883845782
100	0.0022301383546922443
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4528867.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.54756159297074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67298860969441	55.41062082628778
2	13.7548660410973	19.132347863925517
3	3.6192373174130834	7.551273907570937
4	1.271535797994071	3.5372885691463933
5	0.5494665169713215	1.910702821616904
6	0.30123489183976043	1.2570091316506569
7	0.17349383142571165	0.8446251048956114
8	0.11798927674283642	0.6564693193265988
9	0.0889277560594804	0.556623773268425
>10	0.41106040802573723	5.014043370107162
>50	0.023505814753336372	1.1347244980297397
>100	0.01454822085988242	1.8518778556245639
>500	9.545978141760165E-4	0.4337475722499615
>1k	1.5909942398861196E-4	0.1643591681983769
>5k	0.0	0.0
>10k+	3.181988479771801E-5	0.5442862181013369
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24595	0.5430718102342154	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.624173330769043E-5	0.0
5	0.0	0.0	0.0	6.624173330769043E-5	0.0
6	0.0	0.0	0.0	6.624173330769043E-5	0.0
7	0.0	0.0	0.0	6.624173330769043E-5	0.0
8	2.2080577769230142E-5	0.0	0.0	8.832231107692057E-5	0.0
9	4.4161155538460285E-5	0.0	0.0	8.832231107692057E-5	0.0
10-11	4.4161155538460285E-5	0.0	0.0	9.936259996153564E-5	0.0
12-13	4.4161155538460285E-5	0.0	0.0	1.2144317773076578E-4	0.0
14-15	6.624173330769043E-5	0.0	0.0	1.8768491103845618E-4	0.0
16-17	8.832231107692057E-5	0.0	0.0	1.9872519992307125E-4	0.0
18-19	8.832231107692057E-5	0.0	0.0	2.0976548880768633E-4	0.0
20-21	8.832231107692057E-5	0.0	0.0	2.7600722211537675E-4	0.0
22-23	8.832231107692057E-5	0.0	0.0	7.617799330384399E-4	0.0
24-25	8.832231107692057E-5	0.0	0.0	0.0014573181327691893	0.0
26-27	8.832231107692057E-5	0.0	0.0	0.002528226154576851	0.0
28-29	8.832231107692057E-5	0.0	0.0	0.005895514264384448	0.0
30-31	8.832231107692057E-5	0.0	0.0	0.014816067683153425	0.0
32-33	9.936259996153564E-5	0.0	0.0	0.03453402363107594	0.0
34-35	1.104028888461507E-4	0.0	0.0	0.06646253908538273	0.0
36-37	1.3248346661538085E-4	0.0	0.0	0.11195956957888142	0.0
38-39	1.54564044384611E-4	0.0	0.0	0.17453592697687964	0.0
40-41	1.54564044384611E-4	0.0	0.0	0.24447615706091613	0.0
42-43	1.7664462215384114E-4	0.0	0.0	0.318534414898914	0.0
44-45	1.9872519992307125E-4	0.0	0.0	0.4091420657749499	0.0
46-47	1.9872519992307125E-4	0.0	0.0	0.515603571489293	0.0
48-49	1.9872519992307125E-4	0.0	0.0	0.6311291543779051	0.0
50-51	1.9872519992307125E-4	0.0	0.0	0.7502207505762479	0.0
52-53	1.9872519992307125E-4	0.0	0.0	0.8690252992635907	0.0
54-55	1.9872519992307125E-4	0.0	0.0	1.005626793632933	0.0
56-57	1.9872519992307125E-4	0.0	0.0	1.1703920649469284	0.0
58-59	1.9872519992307125E-4	0.0	0.0	1.3490681002555385	0.0
60-61	1.9872519992307125E-4	0.0	0.0	1.5307360538518795	0.0
62-63	2.0976548880768633E-4	0.0	0.0	1.7156498523802974	1.1040288884615071E-5
64-65	2.208057776923014E-4	0.0	0.0	1.9263427254542913	2.2080577769230142E-5
66-67	2.208057776923014E-4	0.0	0.0	2.176559832735207	2.2080577769230142E-5
68-69	2.208057776923014E-4	0.0	0.0	2.443017204965392	2.2080577769230142E-5
70-71	2.208057776923014E-4	0.0	0.0	2.734425630074807	2.2080577769230142E-5
72-73	2.208057776923014E-4	0.0	0.0	3.0578950541051437	2.2080577769230142E-5
74-75	2.208057776923014E-4	0.0	0.0	3.3851623375117885	2.2080577769230142E-5
76-77	2.208057776923014E-4	0.0	0.0	3.7339250633767787	2.2080577769230142E-5
78-79	2.208057776923014E-4	0.0	0.0	4.12352581782596	2.2080577769230142E-5
80-81	2.208057776923014E-4	0.0	0.0	4.551149327193755	2.2080577769230142E-5
82-83	2.208057776923014E-4	0.0	0.0	4.999981231508896	2.2080577769230142E-5
84-85	2.208057776923014E-4	0.0	0.0	5.445015276447729	2.2080577769230142E-5
86-87	2.208057776923014E-4	0.0	0.0	5.921469983552178	2.2080577769230142E-5
88	2.208057776923014E-4	0.0	0.0	6.3076040872915895	2.2080577769230142E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5590	0.0	39.77795	1
TACCTGG	4570	0.0	34.87005	2
GTATCAA	9170	0.0	34.142708	1
GTACCTG	5285	0.0	30.42105	1
TCAACGC	10120	0.0	30.145916	4
ATCAACG	10210	0.0	30.018301	3
TCTGTCG	1310	0.0	29.419796	8
TATCAAC	11170	0.0	27.564949	2
CAACGCA	11305	0.0	27.068558	5
AACGCAG	11955	0.0	25.594	6
GTCTCGC	1365	0.0	25.14104	1
CGCAGAG	12270	0.0	23.978771	8
GTACATG	16495	0.0	23.939774	1
CTGTCGC	1650	0.0	23.927235	9
ACGCAGA	12770	0.0	23.408466	7
TACATGG	16535	0.0	23.084522	2
CATGGGT	7900	0.0	23.02757	4
ACCTGGG	6825	0.0	22.659874	3
ACATGGG	17225	0.0	21.177113	3
TCGCTCT	1645	0.0	21.146091	4
>>END_MODULE