FastQCFastQC Report
Wed 25 May 2016
SRR2431460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4528867
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255670.564534131825907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230750.5095093320249855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153180.3382302902690673No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109170.24105366750668544No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64760.14299382163353438No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58800.12983379728307323No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57330.1265879523509964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47990.10596469271453544No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107550.058.969721
GTATCAA199300.041.5956731
GTGGTAT39900.035.9647061
TGGTATC39850.034.931412
TACCTGG43200.034.6175352
ATCAACG262250.031.3995513
TCAACGC265550.031.0624774
TATCAAC266900.030.958222
GTACCTG48950.030.7572021
CAACGCA277200.029.7255865
TATAACG4700.029.0170272
AACGCAG288450.028.7608386
ATAACGC4500.028.2165573
CGCAGAG321300.025.4106888
ACGCAGA325000.025.367267
GCAGAGT343450.023.4297479
ACCTGGG62300.023.24963
GTACATG153800.022.9432261
CATGGGT74000.022.5605374
GTCTCGC13900.022.3397671
Produced by FastQC (version 0.11.5)