##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431460_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4528867
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.271013699452865	33.0	31.0	34.0	30.0	34.0
2	32.381007435192956	34.0	31.0	34.0	31.0	34.0
3	32.48732078023046	34.0	31.0	34.0	31.0	34.0
4	36.04505210685145	37.0	35.0	37.0	35.0	37.0
5	35.96711075860696	37.0	35.0	37.0	35.0	37.0
6	36.00975475764689	37.0	35.0	37.0	35.0	37.0
7	35.94221269911437	37.0	35.0	37.0	35.0	37.0
8	35.92058366916052	37.0	35.0	37.0	35.0	37.0
9	37.608407798241814	39.0	37.0	39.0	35.0	39.0
10-11	37.54495086740238	39.0	37.0	39.0	34.5	39.0
12-13	37.54350414794693	39.0	37.0	39.0	35.0	39.0
14-15	38.890252793910705	40.0	38.0	41.0	35.0	41.0
16-17	38.936865776804666	40.0	38.0	41.0	35.0	41.0
18-19	39.01093386049976	40.5	38.5	41.0	35.0	41.0
20-21	39.02474515149153	41.0	39.0	41.0	35.0	41.0
22-23	38.95753419122266	40.0	38.5	41.0	35.0	41.0
24-25	38.90293510496113	40.5	38.0	41.0	35.0	41.0
26-27	38.76707629082506	40.0	38.0	41.0	34.5	41.0
28-29	38.66593134662599	40.0	38.0	41.0	34.0	41.0
30-31	38.47983678920136	40.0	38.0	41.0	34.0	41.0
32-33	38.25823887078159	40.0	38.0	41.0	34.0	41.0
34-35	38.096996886859344	40.0	38.0	41.0	33.5	41.0
36-37	37.93653213927457	40.0	37.0	41.0	33.0	41.0
38-39	37.80928287361938	40.0	37.0	41.0	33.0	41.0
40-41	37.676969648258606	40.0	37.0	41.0	33.0	41.0
42-43	37.549147612416085	40.0	37.0	41.0	33.0	41.0
44-45	37.33070754782598	40.0	37.0	41.0	32.5	41.0
46-47	37.00362353321482	40.0	37.0	41.0	31.0	41.0
48-49	36.745880150598374	40.0	37.0	41.0	31.0	41.0
50-51	36.61873930058003	40.0	36.5	41.0	30.0	41.0
52-53	36.48313717316053	40.0	36.0	41.0	30.0	41.0
54-55	36.32589707315317	40.0	36.0	41.0	30.0	41.0
56-57	36.120128500130384	40.0	35.0	41.0	29.5	41.0
58-59	35.96326862767222	39.0	35.0	41.0	29.0	41.0
60-61	35.75050260915147	39.0	35.0	41.0	28.5	41.0
62-63	35.51009557136476	39.0	35.0	41.0	28.0	41.0
64-65	35.261700884570025	38.0	35.0	41.0	28.0	41.0
66-67	34.99992547805003	37.5	35.0	40.0	28.0	41.0
68-69	34.70042761246908	37.0	35.0	40.0	28.0	41.0
70-71	34.359516850461716	36.5	34.0	39.5	27.5	41.0
72-73	34.03212425977623	36.0	34.0	39.0	26.0	41.0
74-75	33.72309840408208	36.0	34.0	39.0	26.0	41.0
76-77	33.369147515261545	35.0	34.0	38.5	26.0	40.0
78-79	33.05694801812462	35.0	34.0	37.0	26.0	39.0
80-81	32.75291988040276	35.0	34.0	37.0	26.0	39.0
82-83	32.495281932545154	35.0	34.0	36.0	26.0	39.0
84-85	32.263721699047466	35.0	33.5	36.0	25.5	37.0
86-87	32.018163704078745	35.0	33.0	36.0	25.0	37.0
88-89	31.869196754949968	35.0	33.0	35.0	25.0	37.0
90-91	31.68487648235199	35.0	33.0	35.0	25.0	36.0
92-93	31.48754622292949	35.0	33.0	35.0	24.0	36.0
94-95	31.328238056008267	35.0	33.0	35.0	24.0	36.0
96-97	31.167766242638614	35.0	33.0	35.0	23.0	36.0
98-99	31.008323052984334	35.0	33.0	35.0	20.0	35.5
100	30.902391922748006	35.0	33.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1846541365902823
1101	2	0.26067321145967526
1101	3	0.25031466179132167
1101	4	0.15453033518528514
1101	5	0.11091320402888272
1101	6	0.2085513273255799
1101	7	0.19921790918257187
1101	8	0.2569989198546523
1101	9	0.33892735555831166
1101	10-11	0.3591490965494586
1101	12-13	0.34741045126347103
1101	14-15	0.44503050378852294
1101	16-17	0.4219676028593824
1101	18-19	0.41452882427181237
1101	20-21	0.3850163422819719
1101	22-23	0.45714372728950536
1101	24-25	0.39848613814884004
1101	26-27	0.4180315586117729
1101	28-29	0.4588035354390314
1101	30-31	0.44921634015592105
1101	32-33	0.435249302893034
1101	34-35	0.515237121063123
1101	36-37	0.5463580739555667
1101	38-39	0.47964227485078936
1101	40-41	0.5060692994048779
1101	42-43	0.4843180188953795
1101	44-45	0.4599134251421333
1101	46-47	0.4426533160604649
1101	48-49	0.3660433299852528
1101	50-51	0.3151849307450476
1101	52-53	0.35267096618697735
1101	54-55	0.31509430748980094
1101	56-57	0.35644094101412094
1101	58-59	0.3756472367241557
1101	60-61	0.3779933926521153
1101	62-63	0.3962260571626004
1101	64-65	0.3537650885677692
1101	66-67	0.34924162504825773
1101	68-69	0.28441572987873087
1101	70-71	0.38698503308926036
1101	72-73	0.3686596228205872
1101	74-75	0.361486661101317
1101	76-77	0.3693656787492827
1101	78-79	0.3413661318589334
1101	80-81	0.38939055482536844
1101	82-83	0.3640934509600129
1101	84-85	0.34933448852915205
1101	86-87	0.3794427452513638
1101	88-89	0.3404734288456872
1101	90-91	0.35331970904860555
1101	92-93	0.3583290365679481
1101	94-95	0.369807226077949
1101	96-97	0.42604657165591675
1101	98-99	0.4513434112007495
1101	100	0.46781593676661615
1102	1	0.19140400755765796
1102	2	0.2606703012485312
1102	3	0.25185079855378945
1102	4	0.20753351052120905
1102	5	0.17033739647313695
1102	6	0.15306940238551903
1102	7	0.1957305213214724
1102	8	0.2358761526658384
1102	9	0.3017260810586251
1102	10-11	0.2882803055332417
1102	12-13	0.31876415166401273
1102	14-15	0.41910995796387596
1102	16-17	0.4331424385000915
1102	18-19	0.4234410730617668
1102	20-21	0.36497303606658704
1102	22-23	0.3751876911671559
1102	24-25	0.3509669601416192
1102	26-27	0.3968772565569125
1102	28-29	0.4154543489578657
1102	30-31	0.4630716735145839
1102	32-33	0.4823005507790654
1102	34-35	0.507980498212639
1102	36-37	0.4951303320404534
1102	38-39	0.5053969954804103
1102	40-41	0.5739289093642839
1102	42-43	0.5758991918573457
1102	44-45	0.6039776414064733
1102	46-47	0.5819560403841493
1102	48-49	0.4854040711869274
1102	50-51	0.4188901911085736
1102	52-53	0.39791246062313945
1102	54-55	0.3335969411058386
1102	56-57	0.46077357923897466
1102	58-59	0.4214607695379229
1102	60-61	0.4190835732063931
1102	62-63	0.43200874014673474
1102	64-65	0.4255480723949674
1102	66-67	0.3817730906262753
1102	68-69	0.4364207528122179
1102	70-71	0.4604146848622719
1102	72-73	0.4181888359179169
1102	74-75	0.47138764730593863
1102	76-77	0.4610658406957029
1102	78-79	0.420351638166224
1102	80-81	0.41648233558651526
1102	82-83	0.3974700145704162
1102	84-85	0.38063354789661474
1102	86-87	0.36114835088415376
1102	88-89	0.35413284588083016
1102	90-91	0.3563133955046922
1102	92-93	0.40999037720718334
1102	94-95	0.35452145573493965
1102	96-97	0.3651194029409943
1102	98-99	0.46863269139480934
1102	100	0.5017848533925466
1103	1	0.15145224314490946
1103	2	0.1951978274219215
1103	3	0.1848685992378023
1103	4	0.13865548858839816
1103	5	0.14501752543720414
1103	6	0.15501661353617635
1103	7	0.1353593290724504
1103	8	0.18549780458551623
1103	9	0.256885222114704
1103	10-11	0.25610695155899776
1103	12-13	0.24074501965998252
1103	14-15	0.32213547552210287
1103	16-17	0.3248232871460317
1103	18-19	0.29055120602782836
1103	20-21	0.3089358782782483
1103	22-23	0.3060147470891792
1103	24-25	0.3183797213292934
1103	26-27	0.33947209941784706
1103	28-29	0.3055207448386881
1103	30-31	0.33583776249435715
1103	32-33	0.35331647675118916
1103	34-35	0.2899256833505248
1103	36-37	0.32352316027253636
1103	38-39	0.36914864976921535
1103	40-41	0.41042606416494465
1103	42-43	0.405661231433875
1103	44-45	0.4437796496712565
1103	46-47	0.40192230597123313
1103	48-49	0.41714888180992915
1103	50-51	0.32789030884824655
1103	52-53	0.2793749461230135
1103	54-55	0.3334833993302979
1103	56-57	0.40027657102343994
1103	58-59	0.37672030774671583
1103	60-61	0.3467757082023013
1103	62-63	0.3741067206721098
1103	64-65	0.3927547726135856
1103	66-67	0.3758352028364911
1103	68-69	0.35186536069952723
1103	70-71	0.4425861690607462
1103	72-73	0.35674152546090454
1103	74-75	0.4043093368877706
1103	76-77	0.38566709421012746
1103	78-79	0.3892492459175827
1103	80-81	0.32419451166990143
1103	82-83	0.34992843696049647
1103	84-85	0.2987112452002165
1103	86-87	0.34890762195851366
1103	88-89	0.2798666744744658
1103	90-91	0.3220644031177038
1103	92-93	0.3213176551113719
1103	94-95	0.34762687519131674
1103	96-97	0.31809287859059765
1103	98-99	0.398033440049943
1103	100	0.36284098957022337
1104	1	0.15619335503846798
1104	2	0.18994488888318983
1104	3	0.18166260370578868
1104	4	0.17492781227259968
1104	5	0.1296651641662976
1104	6	0.12660309156895977
1104	7	0.13577006075892228
1104	8	0.14697793059342246
1104	9	0.25943791794212956
1104	10-11	0.26039250523054847
1104	12-13	0.2504096399682112
1104	14-15	0.37107744417136956
1104	16-17	0.32730571695127963
1104	18-19	0.31299468251472007
1104	20-21	0.2908703781123805
1104	22-23	0.24074789949240483
1104	24-25	0.2527412369444022
1104	26-27	0.24324701126772652
1104	28-29	0.3582993174296263
1104	30-31	0.3428258900281591
1104	32-33	0.3223639892217278
1104	34-35	0.39495821263709985
1104	36-37	0.42971007978254505
1104	38-39	0.443168487949265
1104	40-41	0.4169927666593338
1104	42-43	0.42142789597114216
1104	44-45	0.4185833298699464
1104	46-47	0.43345481165759026
1104	48-49	0.4164914223910472
1104	50-51	0.37787927437734936
1104	52-53	0.41888201121060575
1104	54-55	0.41628565620093383
1104	56-57	0.4440089306000914
1104	58-59	0.5122312668401676
1104	60-61	0.44724385463938887
1104	62-63	0.40593271612328863
1104	64-65	0.4684012836430682
1104	66-67	0.4309385638236165
1104	68-69	0.4222942533350391
1104	70-71	0.42289073457281745
1104	72-73	0.4144886147890432
1104	74-75	0.3958601979283074
1104	76-77	0.3794821337223908
1104	78-79	0.42644152944866676
1104	80-81	0.43853147705759454
1104	82-83	0.4405524965469425
1104	84-85	0.42841937077329106
1104	86-87	0.3889228651192589
1104	88-89	0.40779203632522965
1104	90-91	0.42931271276059135
1104	92-93	0.46677519684341107
1104	94-95	0.47325113442265376
1104	96-97	0.5029150050369715
1104	98-99	0.5050035086493025
1104	100	0.49983627159874544
1105	1	0.06503858318673394
1105	2	0.12549892380938843
1105	3	0.10982061596976678
1105	4	0.12673591405094697
1105	5	0.14424147942173704
1105	6	0.1529144523479502
1105	7	0.1843776687290486
1105	8	0.18000216183460793
1105	9	0.22897785848985563
1105	10-11	0.20437316609804412
1105	12-13	0.14124909404881691
1105	14-15	0.2778864003874091
1105	16-17	0.25478712222364663
1105	18-19	0.19406271642689887
1105	20-21	0.18326431395270504
1105	22-23	0.27489463921099855
1105	24-25	0.22359881695862782
1105	26-27	0.2516198617767813
1105	28-29	0.23321121107026244
1105	30-31	0.26568852499585915
1105	32-33	0.28736793278833517
1105	34-35	0.27983267794855493
1105	36-37	0.2953123098533794
1105	38-39	0.35260468034621795
1105	40-41	0.39548530609567933
1105	42-43	0.4413056938932627
1105	44-45	0.4586615468906672
1105	46-47	0.41616701029865055
1105	48-49	0.4295320118359669
1105	50-51	0.4535336313257048
1105	52-53	0.4333294748753289
1105	54-55	0.4596539976373748
1105	56-57	0.4940686951598394
1105	58-59	0.43178729393930126
1105	60-61	0.476808634305371
1105	62-63	0.4256492819996751
1105	64-65	0.3991774211578729
1105	66-67	0.4087320016961087
1105	68-69	0.42024718442701925
1105	70-71	0.43316037600493473
1105	72-73	0.38499699484451355
1105	74-75	0.3320502071923457
1105	76-77	0.3947024660677627
1105	78-79	0.3689274218017573
1105	80-81	0.36022808053007793
1105	82-83	0.31485222993599393
1105	84-85	0.35342216322099773
1105	86-87	0.35589447636170135
1105	88-89	0.35459439413340377
1105	90-91	0.3595653069094311
1105	92-93	0.37842093640179897
1105	94-95	0.4027344728458928
1105	96-97	0.3981435397327857
1105	98-99	0.45280826774259
1105	100	0.5588475354520988
1106	1	0.07237197458787392
1106	2	0.09909772106973236
1106	3	0.06689981759468822
1106	4	0.10466272571537871
1106	5	0.0754908532504146
1106	6	0.12011908452316078
1106	7	0.08673399486892919
1106	8	0.10536290807952042
1106	9	0.14128809024173705
1106	10-11	0.14397339583391755
1106	12-13	0.1674835460870554
1106	14-15	0.1877250495830296
1106	16-17	0.16774770317978493
1106	18-19	0.1743694084961902
1106	20-21	0.14378314366559408
1106	22-23	0.20344390095245046
1106	24-25	0.1573391463175966
1106	26-27	0.21614677068434673
1106	28-29	0.16311683657298914
1106	30-31	0.17005076669124009
1106	32-33	0.1465542392546766
1106	34-35	0.14752882138748902
1106	36-37	0.129442889627704
1106	38-39	0.07553904082543283
1106	40-41	0.09844276692956555
1106	42-43	0.182364192300696
1106	44-45	0.1623168539405455
1106	46-47	0.1094848677438236
1106	48-49	0.007703975963153198
1106	50-51	-0.025684463175153383
1106	52-53	-0.02236172216795751
1106	54-55	-0.026507380974123862
1106	56-57	0.009364787809886366
1106	58-59	0.01577230549356301
1106	60-61	0.041280061877841945
1106	62-63	0.00989446390526183
1106	64-65	-0.006547737373367113
1106	66-67	-0.018486750770236426
1106	68-69	0.037038165269962064
1106	70-71	0.04107076511239427
1106	72-73	0.010281118165814007
1106	74-75	0.017193078530851835
1106	76-77	0.04681880246355519
1106	78-79	-0.022414535418626258
1106	80-81	0.007454678284162242
1106	82-83	-0.06753264552696692
1106	84-85	-0.05503382812934632
1106	86-87	-0.01198640039674359
1106	88-89	-0.00847148583716617
1106	90-91	-0.0015675285129077565
1106	92-93	0.008853956618803949
1106	94-95	0.05344074298305301
1106	96-97	0.05087515973869827
1106	98-99	0.09453143806486963
1106	100	0.11425242140815328
1107	1	0.0896507072512236
1107	2	0.12886667006944919
1107	3	0.10906349437374985
1107	4	0.08543821802933138
1107	5	0.10100195183843397
1107	6	0.10088205374766801
1107	7	0.07280205786006633
1107	8	0.06655072311348675
1107	9	0.10405224915974287
1107	10-11	0.10238499847628901
1107	12-13	0.019796404169149184
1107	14-15	0.19760420176987026
1107	16-17	0.17624070590355245
1107	18-19	0.10011688039750766
1107	20-21	0.13249749199532346
1107	22-23	0.11254349082128101
1107	24-25	0.13051860218935474
1107	26-27	0.13605378674520807
1107	28-29	0.12848814617507287
1107	30-31	0.1797358109916658
1107	32-33	0.12655173463678437
1107	34-35	0.1876747153795364
1107	36-37	0.1479207081055307
1107	38-39	0.1283809994366294
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2216	70-71	-1.123476103380142
2216	72-73	-1.0479041904884454
2216	74-75	-1.023114414752058
2216	76-77	-0.9766935333870705
2216	78-79	-0.8582712449493357
2216	80-81	-0.9356116117096818
2216	82-83	-1.0432061509061619
2216	84-85	-1.0128754965466982
2216	86-87	-1.0785876929521123
2216	88-89	-0.9816104198751958
2216	90-91	-0.9623902840478245
2216	92-93	-0.941073574405106
2216	94-95	-1.0847122149305797
2216	96-97	-1.2629440704776869
2216	98-99	-1.3162216127269915
2216	100	-1.3084479209881792
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	233.0
3	474.0
4	351.0
5	320.0
6	602.0
7	1346.0
8	2385.0
9	3978.0
10	5652.0
11	8075.0
12	11623.0
13	16382.0
14	17641.0
15	21552.0
16	26796.0
17	37736.0
18	41342.0
19	22964.0
20	14998.0
21	13879.0
22	13885.0
23	14542.0
24	16894.0
25	20591.0
26	25997.0
27	33291.0
28	42184.0
29	53863.0
30	68670.0
31	86418.0
32	108305.0
33	139172.0
34	186136.0
35	275343.0
36	426022.0
37	828043.0
38	1291314.0
39	645888.0
40	3980.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.437884706001384	21.194736706069428	14.06753439779476	24.29984419013443
2	14.866278917000653	21.815434191377225	39.04437909084104	24.27390780078108
3	16.151721150196487	26.86929450914921	31.592755882770522	25.38622845788378
4	11.473041592617855	18.767929423998023	38.409021658600565	31.35000732478355
5	11.339760684545807	37.47489051414025	34.98983254895977	16.19551625235417
6	27.867212704634515	39.27825657057273	18.373822856798398	14.480707867994358
7	24.642322240860683	33.76451549581827	23.191120428133573	18.402041835187475
8	24.579370513640608	34.11122472795072	21.824708034040302	19.48469672436837
9	24.552101000095607	16.00342867211601	23.1692827367198	36.27518759106858
10-11	22.488707666862094	26.922997936409615	29.62598114392908	20.962313252799206
12-13	24.37907759269592	25.030984570754672	29.51419196015251	21.0757458763969
14-15	21.879037295641492	26.814697804108622	28.191201022242428	23.115063878007458
16-17	19.613823943162824	29.860890151996074	28.662875284259837	21.86241062058126
18-19	19.24755131912684	27.289728313946952	32.762609279539454	20.70011108738676
20-21	21.0811997791059	26.884019778015116	32.1851911305852	19.84958931229378
22-23	20.545900332246454	27.216155828819875	32.2082984552207	20.029645383712968
24-25	20.252232136942673	26.864725302969145	32.54725709253841	20.335785467549776
26-27	20.060447789701044	26.535378495327862	32.71405408902491	20.69011962594618
28-29	20.151649893654078	26.737181217537998	32.62635513079748	20.484813758010446
30-31	20.77029458369473	27.49816945098545	31.906921992555915	19.824613972763906
32-33	19.820210905079467	26.970252129563676	32.71989740469019	20.489639560666674
34-35	19.982564871661026	26.38407891912803	32.60230993369528	21.031046275515656
36-37	20.91412048128921	27.21438082262563	31.56212530389717	20.309373392187993
38-39	20.693391032195937	27.112982001196347	31.860507891603095	20.33311907500462
40-41	20.256704468585845	26.91298728268791	32.007552066744	20.822756181982243
42-43	20.16244084784734	27.007110292446963	31.843328025804702	20.987120833901
44-45	20.80608911744252	27.23796340651684	31.33763161267836	20.618315863362277
46-47	20.63311422698605	27.993700830519707	30.53936859481577	20.833816347678475
48-49	20.604043435784366	27.408215314871015	30.707609542518167	21.280131706826452
50-51	20.6634474172954	27.132524539066537	30.55566166659196	21.6483663770461
52-53	20.73546090842156	27.474514132612736	30.201993515548757	21.58803144341694
54-55	21.023932934436374	28.200589468961248	29.8459290296682	20.929548566934184
56-57	20.511385755897464	27.671252266430418	30.001709138614014	21.8156528390581
58-59	20.259452211610824	26.98610389748047	30.715227061537863	22.03921682937084
60-61	20.937193145854074	27.697296934456013	29.83958738930066	21.525922530389256
62-63	20.775609230998533	27.48785126179613	30.07318224349367	21.663357263711667
64-65	20.60312392597587	27.13433884164762	30.54518279097173	21.717354441404776
66-67	20.54746412068548	27.346201463811116	29.97920228543014	22.127132130073264
68-69	21.276395139109006	27.560818271255084	29.735204849894803	21.42758173974111
70-71	21.41195810638567	27.12351528339684	29.779706890431957	21.68481971978553
72-73	20.909810334461135	27.24780833705207	30.283457209054713	21.55892411943208
74-75	20.881966854756886	27.22807420418627	29.98060216103187	21.909356780024975
76-77	20.95599186286548	27.41389844303222	29.526678526881007	22.103431167221295
78-79	21.19452221760981	27.552662491940538	29.617992686869044	21.63482260358061
80-81	20.80920018185564	27.2073125574233	29.830220229474612	22.153267031246447
82-83	20.588855830215245	27.23134178653518	30.03358603732344	22.14621634592614
84-85	20.75077495001204	27.206213157650396	29.7471836334267	22.295828258910863
86-87	20.965557382355758	27.33119063359349	29.52499588197134	22.178256102079416
88-89	20.440068122998532	27.237176538856183	29.71237618592023	22.61037915222505
90-91	20.22562155170377	27.01654740578604	29.6502524803665	23.107578562143686
92-93	20.569905449642754	27.337673428160063	29.368053503130426	22.724367619066754
94-95	20.370370779269958	26.94351589481431	29.742405771686386	22.94370755422935
96-97	20.186645595830555	27.004324828121394	29.74388313325865	23.0651464427894
98-99	20.17375959226677	26.899891771486473	29.70757324690067	23.21877538934609
100	20.430506165465665	27.076225668518866	29.312000494606256	23.18126767140922
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4162.0
1	2447.5
2	4013.0
3	6979.5
4	4339.0
5	2013.5
6	3456.5
7	7960.0
8	12584.5
9	13672.5
10	9710.5
11	5343.5
12	4411.5
13	4648.0
14	5202.5
15	5692.5
16	6123.5
17	6716.5
18	7455.5
19	8263.0
20	9454.0
21	11054.0
22	13358.0
23	17192.5
24	22185.5
25	28409.5
26	37105.5
27	48404.0
28	63513.0
29	78139.5
30	89220.0
31	100357.0
32	111592.5
33	121677.0
34	130899.5
35	138729.0
36	143910.0
37	147400.0
38	149823.5
39	151002.0
40	151743.5
41	150006.0
42	144459.0
43	140599.0
44	138624.5
45	136403.5
46	135696.0
47	135931.0
48	136546.5
49	137533.0
50	137915.0
51	133905.0
52	125915.5
53	118569.0
54	113009.0
55	108685.5
56	101520.5
57	92595.0
58	86037.5
59	78691.5
60	66834.0
61	53765.0
62	43210.5
63	33738.0
64	25286.0
65	18553.0
66	13636.5
67	9990.5
68	7087.0
69	4803.5
70	3249.0
71	2300.5
72	1713.0
73	1210.5
74	838.0
75	614.0
76	373.5
77	267.5
78	204.0
79	124.0
80	67.5
81	30.0
82	15.0
83	7.5
84	4.5
85	1.0
86	1.5
87	2.5
88	1.0
89	0.5
90	1.5
91	2.0
92	1.0
93	0.0
94	0.5
95	2.0
96	2.5
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04848894878122939
2	0.0
3	1.3248346661538085E-4
4	0.06924469188430572
5	0.004416115553846028
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.00777236337476901
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.078532886922932E-4
26-27	0.0
28-29	3.2016837765383704E-4
30-31	0.003775778798538354
32-33	1.1040288884615071E-4
34-35	0.001744365643769181
36-37	0.004349873820538338
38-39	0.0014573181327691893
40-41	8.942633996538206E-4
42-43	0.0017664462215384114
44-45	1.104028888461507E-5
46-47	3.532892443076822E-4
48-49	4.526518442692179E-4
50-51	0.0015014792883076495
52-53	7.396993552692097E-4
54-55	9.936259996153564E-4
56-57	0.005961755997692138
58-59	0.008898472840999747
60-61	0.0010157065773845864
62-63	0.0023074203768845497
64-65	0.0010267468662692016
66-67	8.832231107692056E-5
68-69	5.851353108845987E-4
70-71	3.2016837765383704E-4
72-73	0.0
74-75	2.649669332307617E-4
76-77	0.0
78-79	5.961755997692138E-4
80-81	0.0
82-83	0.0011150691773461222
84-85	5.851353108845987E-4
86-87	2.208057776923014E-5
88-89	0.0
90-91	0.0
92-93	2.3184606657691647E-4
94-95	0.0
96-97	3.643295331922973E-4
98-99	5.188935775769084E-4
100	2.649669332307617E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4528867.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.57175041615477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.779749661716	49.57802832171169
2	15.48496396214793	19.997824563339346
3	4.153767246584452	8.046480657997488
4	1.5541609889315866	4.0141958193525875
5	0.6536778922973164	2.110456285699021
6	0.3609096115185402	1.3982739214659934
7	0.2161337096562952	0.9769292369510768
8	0.14516308189175772	0.7498747434843532
9	0.09983385210313556	0.5801801922978278
>10	0.4989946599809705	5.650142472217829
>50	0.030654076920287827	1.3755574738327871
>100	0.020042474608246333	2.465031586303954
>500	0.0013333772231966952	0.5937042604490307
>1k	3.7608047901260263E-4	0.41095446764336746
>5k	1.0256740336682617E-4	0.39934221677088694
>10k+	1.3675653782243487E-4	1.6530237804828183
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25567	0.564534131825907	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23075	0.5095093320249855	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15318	0.3382302902690673	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10917	0.24105366750668544	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6476	0.14299382163353438	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5880	0.12983379728307323	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5733	0.1265879523509964	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4799	0.10596469271453544	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.2080577769230142E-5	0.0
5	0.0	0.0	0.0	2.2080577769230142E-5	0.0
6	0.0	0.0	0.0	2.2080577769230142E-5	0.0
7	0.0	0.0	0.0	2.2080577769230142E-5	0.0
8	0.0	0.0	0.0	2.2080577769230142E-5	0.0
9	0.0	0.0	0.0	1.104028888461507E-4	0.0
10-11	0.0	0.0	0.0	1.3248346661538085E-4	0.0
12-13	0.0	0.0	0.0	1.3248346661538085E-4	0.0
14-15	2.2080577769230142E-5	0.0	0.0	1.8768491103845618E-4	0.0
16-17	2.2080577769230142E-5	0.0	0.0	1.9872519992307125E-4	0.0
18-19	2.2080577769230142E-5	0.0	0.0	2.0976548880768633E-4	0.0
20-21	2.2080577769230142E-5	0.0	0.0	2.9808779988460695E-4	0.0
22-23	2.2080577769230142E-5	0.0	0.0	7.728202219230549E-4	0.0
24-25	6.624173330769043E-5	0.0	0.0	0.0014793987105384196	0.0
26-27	6.624173330769043E-5	0.0	0.0	0.0025944678878845415	0.0
28-29	6.624173330769043E-5	0.0	0.0	0.006039038019884443	0.0
30-31	6.624173330769043E-5	0.0	0.0	0.015191437505230337	0.0
32-33	6.624173330769043E-5	0.0	0.0	0.035284763275229766	0.0
34-35	6.624173330769043E-5	0.0	0.0	0.06778737375153653	0.0
36-37	6.624173330769043E-5	0.0	0.0	0.11401306331141983	0.0
38-39	8.832231107692057E-5	0.0	0.0	0.17814610144214876	0.0
40-41	8.832231107692057E-5	0.0	0.0	0.2504710339252621	0.0
42-43	8.832231107692057E-5	0.0	0.0	0.32713479994002914	0.0
44-45	9.936259996153564E-5	0.0	0.0	0.4200609114818342	0.0
46-47	1.104028888461507E-4	0.0	0.0	0.5295584966394464	0.0
48-49	1.104028888461507E-4	0.0	0.0	0.6482526424379431	0.0
50-51	1.104028888461507E-4	0.0	0.0	0.7703471972129011	0.0
52-53	1.104028888461507E-4	0.0	0.0	0.8918897375436284	0.0
54-55	1.104028888461507E-4	0.0	0.0	1.0315383516451244	0.0
56-57	1.104028888461507E-4	0.0	0.0	1.2011061486239272	0.0
58-59	1.3248346661538085E-4	0.0	0.0	1.3844411858418453	0.0
60-61	1.3248346661538085E-4	0.0	0.0	1.5705362952809168	0.0
62-63	1.3248346661538085E-4	0.0	0.0	1.761290406629296	0.0
64-65	1.3248346661538085E-4	0.0	0.0	1.9780002371454053	0.0
66-67	1.3248346661538085E-4	0.0	0.0	2.235305209890244	0.0
68-69	1.3248346661538085E-4	0.0	0.0	2.5077684992736593	0.0
70-71	1.3248346661538085E-4	0.0	0.0	2.805977742335997	0.0
72-73	1.3248346661538085E-4	0.0	0.0	3.1374734563854494	0.0
74-75	1.3248346661538085E-4	0.0	0.0	3.47272286865567	0.0
76-77	1.3248346661538085E-4	0.0	0.0	3.8291696355843525	0.0
78-79	1.3248346661538085E-4	0.0	0.0	4.227403895941302	0.0
80-81	1.3248346661538085E-4	0.0	0.0	4.665316954549559	0.0
82-83	1.3248346661538085E-4	0.0	0.0	5.124725455616161	0.0
84-85	1.3248346661538085E-4	0.0	0.0	5.58094331319511	0.0
86-87	1.3248346661538085E-4	0.0	0.0	6.071783516716211	0.0
88	1.3248346661538085E-4	0.0	0.0	6.468968949629123	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10755	0.0	58.96972	1
GTATCAA	19930	0.0	41.595673	1
GTGGTAT	3990	0.0	35.964706	1
TGGTATC	3985	0.0	34.93141	2
TACCTGG	4320	0.0	34.617535	2
ATCAACG	26225	0.0	31.399551	3
TCAACGC	26555	0.0	31.062477	4
TATCAAC	26690	0.0	30.95822	2
GTACCTG	4895	0.0	30.757202	1
CAACGCA	27720	0.0	29.725586	5
TATAACG	470	0.0	29.017027	2
AACGCAG	28845	0.0	28.760838	6
ATAACGC	450	0.0	28.216557	3
CGCAGAG	32130	0.0	25.410688	8
ACGCAGA	32500	0.0	25.36726	7
GCAGAGT	34345	0.0	23.429747	9
ACCTGGG	6230	0.0	23.2496	3
GTACATG	15380	0.0	22.943226	1
CATGGGT	7400	0.0	22.560537	4
GTCTCGC	1390	0.0	22.339767	1
>>END_MODULE