FastQCFastQC Report
Wed 25 May 2016
SRR2431459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074507
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA671561.6481994017926587No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64720.15884130276374542No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57950.14222579566067747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG45750.045.7206882
GGTATCA75700.040.054411
GTACCTG55500.038.2005841
ACCTGGG63250.032.3275343
GTATCAA117500.031.166361
TCTGTCG12450.027.1809338
GTCTCGC9950.026.457661
ATCAACG133250.026.2099043
TCAACGC133000.026.0471214
GTACATG217850.025.5278191
TACATGG214050.025.1000652
TATCAAC145050.024.8230342
CATGGGT105100.024.5982274
CAACGCA145600.023.8898935
ACATGGG224000.023.1037853
GTACACG4350.022.6942861
AACGCAG154750.022.3853936
CTGTCGC14350.022.271959
ATGGGTG57150.020.9733495
TATAACG4050.020.89112
Produced by FastQC (version 0.11.5)