##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431459_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4074507
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.429705974244246	31.0	30.0	34.0	23.0	34.0
2	29.46310338894988	31.0	30.0	34.0	22.0	34.0
3	29.57701434799351	31.0	30.0	34.0	23.0	34.0
4	32.73828023856628	37.0	35.0	37.0	20.0	37.0
5	32.983735701030824	37.0	35.0	37.0	25.0	37.0
6	32.93999666708144	37.0	35.0	37.0	24.0	37.0
7	32.81851497616767	37.0	35.0	37.0	22.0	37.0
8	32.71339060161144	37.0	35.0	37.0	21.0	37.0
9	33.924470371507525	39.0	35.0	39.0	17.0	39.0
10-11	33.90266699750424	39.0	35.0	39.0	17.0	39.0
12-13	33.87246947912962	39.0	35.0	39.0	17.0	39.0
14-15	35.0129713852498	40.0	36.0	41.0	17.0	41.0
16-17	35.036585039613385	40.0	35.5	41.0	17.0	41.0
18-19	35.01966336049981	40.0	35.0	41.0	17.5	41.0
20-21	35.12856217942441	40.0	35.0	41.0	20.0	41.0
22-23	35.10464370290688	40.0	35.0	41.0	22.5	41.0
24-25	35.16287099273605	40.0	35.0	41.0	24.0	41.0
26-27	35.169469091598074	40.0	35.0	41.0	25.0	41.0
28-29	35.06329747378027	40.0	34.5	41.0	24.5	41.0
30-31	35.03830843829695	40.0	34.5	41.0	25.0	41.0
32-33	34.967685538397646	40.0	34.0	41.0	24.0	41.0
34-35	34.85411781106279	40.0	34.0	41.0	24.0	41.0
36-37	34.72116786153515	40.0	34.0	41.0	23.5	41.0
38-39	34.60674996999637	40.0	34.0	41.0	23.0	41.0
40-41	34.51388548356893	39.0	34.0	41.0	22.5	41.0
42-43	34.29897690689941	39.0	33.0	41.0	20.5	41.0
44-45	34.186127425968344	39.0	33.0	41.0	20.0	41.0
46-47	34.05555199684281	39.0	33.0	41.0	18.5	41.0
48-49	33.930236836014764	39.0	33.0	41.0	18.0	41.0
50-51	33.77398934398689	39.0	33.0	41.0	16.0	41.0
52-53	33.676441591583966	38.5	33.0	40.5	15.0	41.0
54-55	33.60779733597218	38.0	33.0	41.0	13.5	41.0
56-57	33.460864467774876	38.0	33.0	40.0	11.0	41.0
58-59	33.260839041385864	38.0	32.0	40.0	8.5	41.0
60-61	33.048996602533755	37.5	32.0	40.0	7.5	41.0
62-63	32.768581573181734	37.0	32.0	40.0	4.0	41.0
64-65	32.61708263110114	37.0	32.0	40.0	2.0	41.0
66-67	32.365881074691984	36.0	31.0	40.0	2.0	41.0
68-69	32.09463378023403	36.0	31.0	39.0	2.0	41.0
70-71	31.794044776460073	35.0	31.0	39.0	2.0	41.0
72-73	31.479231597835025	35.0	31.0	39.0	2.0	41.0
74-75	31.14673566642541	35.0	31.0	38.0	2.0	40.0
76-77	30.822281198682443	35.0	30.5	37.0	2.0	39.5
78-79	30.481036969626018	35.0	30.0	37.0	2.0	39.0
80-81	30.17752331754492	35.0	30.0	36.0	2.0	39.0
82-83	29.874912719502014	35.0	30.0	36.0	2.0	38.0
84-85	29.575400164977015	35.0	30.0	35.5	2.0	37.0
86-87	29.312618066431106	35.0	29.5	35.0	2.0	37.0
88-89	29.03931420414789	34.5	29.0	35.0	2.0	36.5
90-91	28.80788031533631	34.0	29.0	35.0	2.0	36.0
92-93	28.605312004617982	34.0	29.0	35.0	2.0	36.0
94-95	28.368901440100608	34.0	29.0	35.0	2.0	36.0
96-97	28.259545878802022	34.0	29.0	35.0	2.0	35.0
98-99	28.095590951248823	34.0	29.0	35.0	2.0	35.0
100	27.95746724695773	34.0	28.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5126248739704771
1101	2	0.5290103014358039
1101	3	0.5151642349171901
1101	4	0.4775650683669781
1101	5	0.40463447494963134
1101	6	0.35708390216896646
1101	7	0.3450315498204475
1101	8	0.5591877414431181
1101	9	0.6127724613540408
1101	10-11	0.6141017737044834
1101	12-13	0.5640775142932526
1101	14-15	0.6532442644842931
1101	16-17	0.6209731864045409
1101	18-19	0.6423084731585149
1101	20-21	0.5820405553531245
1101	22-23	0.577395271578574
1101	24-25	0.5338676018173132
1101	26-27	0.6795579595076049
1101	28-29	0.7271982003162094
1101	30-31	0.7126945790137498
1101	32-33	0.6993420959536536
1101	34-35	0.7332215243996387
1101	36-37	0.7596532908107463
1101	38-39	0.7130906538759234
1101	40-41	0.7031122165083445
1101	42-43	0.6878854690907374
1101	44-45	0.6415626592812202
1101	46-47	0.5983614832718658
1101	48-49	0.6099341869002544
1101	50-51	0.5821579600074926
1101	52-53	0.5656039146505307
1101	54-55	0.6178489303943735
1101	56-57	0.6763714312039468
1101	58-59	0.6508184827330581
1101	60-61	0.6744940558493084
1101	62-63	0.6200874508251175
1101	64-65	0.6640058396854442
1101	66-67	0.7011302668976498
1101	68-69	0.6141975064392398
1101	70-71	0.617080495270887
1101	72-73	0.5951129918653422
1101	74-75	0.6136577867517978
1101	76-77	0.6590533853803393
1101	78-79	0.6231415500447675
1101	80-81	0.6370524580922705
1101	82-83	0.6196626541703871
1101	84-85	0.6103473156134456
1101	86-87	0.6133411464071514
1101	88-89	0.5941687480963687
1101	90-91	0.6140528415604791
1101	92-93	0.5093413413035961
1101	94-95	0.5935383303230779
1101	96-97	0.6110085190636845
1101	98-99	0.6084743324554189
1101	100	0.5329044988095895
1102	1	0.1216601721155186
1102	2	0.18408774684847273
1102	3	0.16703132974981827
1102	4	0.14795519500294319
1102	5	0.06449800343569478
1102	6	0.03274792984136354
1102	7	0.08596048602218787
1102	8	0.10455596161647662
1102	9	0.1935084418566504
1102	10-11	0.17644469223287018
1102	12-13	0.13760690727347935
1102	14-15	0.19409764574360366
1102	16-17	0.18424403203231776
1102	18-19	0.11208770966401005
1102	20-21	0.21002768993673016
1102	22-23	0.17036623497754277
1102	24-25	0.258554269148199
1102	26-27	0.31397237357678875
1102	28-29	0.34500138448122186
1102	30-31	0.30740017659394425
1102	32-33	0.3587663455774788
1102	34-35	0.35204620116755336
1102	36-37	0.28632654857635487
1102	38-39	0.25549172991114943
1102	40-41	0.31398293133602095
1102	42-43	0.29319928715086263
1102	44-45	0.22737915082392135
1102	46-47	0.2081541191238614
1102	48-49	0.13993486389297516
1102	50-51	0.15744970555020643
1102	52-53	0.2200740464980555
1102	54-55	0.23186676723656774
1102	56-57	0.24919055663153955
1102	58-59	0.2862029762786378
1102	60-61	0.31501392239233184
1102	62-63	0.25027442855078164
1102	64-65	0.20767736281688087
1102	66-67	0.23894439790723965
1102	68-69	0.3130778498916982
1102	70-71	0.24707057030211743
1102	72-73	0.19834486570454857
1102	74-75	0.2020782094507254
1102	76-77	0.14619052366823482
1102	78-79	0.13469526111449426
1102	80-81	0.17232186287515816
1102	82-83	0.17109934768683743
1102	84-85	0.1166142244646835
1102	86-87	0.12738127291537538
1102	88-89	0.20475231582246423
1102	90-91	0.17108622113369165
1102	92-93	0.17982861000839634
1102	94-95	0.15095574359775554
1102	96-97	0.10071069335993243
1102	98-99	0.1306612999623198
1102	100	0.11436818262077608
1103	1	0.42316988601707095
1103	2	0.43165713866494215
1103	3	0.4575664577281202
1103	4	0.42030053745762075
1103	5	0.4174939860589859
1103	6	0.4595716316789549
1103	7	0.5099107989297167
1103	8	0.4957634151536112
1103	9	0.6725254106869514
1103	10-11	0.6535407131032613
1103	12-13	0.5539648894511586
1103	14-15	0.7504233669802289
1103	16-17	0.6013203925782165
1103	18-19	0.48928478455245994
1103	20-21	0.5614095668936798
1103	22-23	0.548537950588063
1103	24-25	0.6285248266104304
1103	26-27	0.6091633780006731
1103	28-29	0.6764514336693992
1103	30-31	0.6656435204980582
1103	32-33	0.7797714550698913
1103	34-35	0.6706953378556335
1103	36-37	0.7187859361073095
1103	38-39	0.7965507159908753
1103	40-41	0.7600181365931249
1103	42-43	0.754609237297629
1103	44-45	0.6886648539719431
1103	46-47	0.6972407148451509
1103	48-49	0.658239944902455
1103	50-51	0.5771853192702849
1103	52-53	0.6064929388169915
1103	54-55	0.7473545347062185
1103	56-57	0.7013829705061525
1103	58-59	0.7358166383235627
1103	60-61	0.7227529520719997
1103	62-63	0.654188515697605
1103	64-65	0.7537647950920672
1103	66-67	0.7377448329102876
1103	68-69	0.6540376127323384
1103	70-71	0.731523395922661
1103	72-73	0.6676914606860507
1103	74-75	0.6418789117225963
1103	76-77	0.6792643087938188
1103	78-79	0.6384145602275382
1103	80-81	0.6029863086118041
1103	82-83	0.590265209288166
1103	84-85	0.5999468188441384
1103	86-87	0.5044435302359709
1103	88-89	0.5220643144365518
1103	90-91	0.5397075443036705
1103	92-93	0.5562368775821014
1103	94-95	0.6263030124802782
1103	96-97	0.644354393755318
1103	98-99	0.5790393574410615
1103	100	0.6585046521241686
1104	1	0.4022603955959809
1104	2	0.42044198288557766
1104	3	0.38226912388453016
1104	4	0.40966512769553987
1104	5	0.35118299933562724
1104	6	0.41091378120735556
1104	7	0.38770091814292584
1104	8	0.4202221176842329
1104	9	0.497349487963632
1104	10-11	0.5349013363402335
1104	12-13	0.5304088642876152
1104	14-15	0.47127040092036765
1104	16-17	0.5786315961050121
1104	18-19	0.4763408783186307
1104	20-21	0.4699012460159864
1104	22-23	0.47171713635849954
1104	24-25	0.4808919191711567
1104	26-27	0.6071583645108163
1104	28-29	0.6129704399447462
1104	30-31	0.5631601978694363
1104	32-33	0.6371232937040716
1104	34-35	0.57750704430903
1104	36-37	0.5917166375297711
1104	38-39	0.6307542609541628
1104	40-41	0.583965334199533
1104	42-43	0.5300519543395055
1104	44-45	0.5345248704222385
1104	46-47	0.5342470478002497
1104	48-49	0.5222305820774764
1104	50-51	0.5724644214267727
1104	52-53	0.5269344433611352
1104	54-55	0.5187373265130049
1104	56-57	0.5478292466382015
1104	58-59	0.5712851189999668
1104	60-61	0.5926398300379532
1104	62-63	0.6408386557832202
1104	64-65	0.7193167875204409
1104	66-67	0.709878225946234
1104	68-69	0.6544100718354926
1104	70-71	0.5662573115337786
1104	72-73	0.6150364163489073
1104	74-75	0.6081416405583866
1104	76-77	0.5527673163140534
1104	78-79	0.5820429175435073
1104	80-81	0.5626346502089277
1104	82-83	0.5426256865951586
1104	84-85	0.554139697532456
1104	86-87	0.5155009388062304
1104	88-89	0.43073389428487374
1104	90-91	0.48916567614167406
1104	92-93	0.45110337494926256
1104	94-95	0.4598853092246671
1104	96-97	0.4937670763500641
1104	98-99	0.5126806536435566
1104	100	0.530524859782183
1105	1	0.24458183675259448
1105	2	0.38345663453275236
1105	3	0.2907841670580247
1105	4	0.37250832157693736
1105	5	0.29486045763538016
1105	6	0.26218675253055324
1105	7	0.3668542077560062
1105	8	0.27503690427082716
1105	9	0.47598478860097515
1105	10-11	0.5139467869100045
1105	12-13	0.41700321145564345
1105	14-15	0.5437802057219372
1105	16-17	0.47217933763749187
1105	18-19	0.4700087349834945
1105	20-21	0.41185669443012785
1105	22-23	0.3678954848038316
1105	24-25	0.4399726409707654
1105	26-27	0.549486686934749
1105	28-29	0.5867318204360146
1105	30-31	0.6117148706000393
1105	32-33	0.6013814948087912
1105	34-35	0.5917884431472871
1105	36-37	0.5510330958224046
1105	38-39	0.5488321995996586
1105	40-41	0.5092365932981338
1105	42-43	0.5308672754725805
1105	44-45	0.49153861505065777
1105	46-47	0.4290861604415994
1105	48-49	0.4565022977846027
1105	50-51	0.5312797065178501
1105	52-53	0.4808552046783845
1105	54-55	0.5798707148582167
1105	56-57	0.5901598876876477
1105	58-59	0.4795742396646858
1105	60-61	0.45288047294918954
1105	62-63	0.5026858938296144
1105	64-65	0.5417645805677367
1105	66-67	0.5510195641926074
1105	68-69	0.5209019070597307
1105	70-71	0.5129922994091949
1105	72-73	0.46758177646933063
1105	74-75	0.5363857047048839
1105	76-77	0.48742938180994244
1105	78-79	0.46375628538380553
1105	80-81	0.43826275954887706
1105	82-83	0.3640968734260994
1105	84-85	0.3610782963112982
1105	86-87	0.38030141132374595
1105	88-89	0.4448121981712809
1105	90-91	0.4272111557516567
1105	92-93	0.33070951967419404
1105	94-95	0.3705450236113208
1105	96-97	0.3873135839605517
1105	98-99	0.41839696085822453
1105	100	0.4281418464918687
1106	1	0.11989634826455386
1106	2	0.1239147634284059
1106	3	0.1261437348301584
1106	4	0.12142001542056136
1106	5	0.08347828567105608
1106	6	0.14851194583333438
1106	7	0.1690330986053965
1106	8	0.23260334509625835
1106	9	0.2338020125971525
1106	10-11	0.27281217933693824
1106	12-13	0.16640477681585253
1106	14-15	0.2606453074275521
1106	16-17	0.18122269708085526
1106	18-19	0.2791039269773776
1106	20-21	0.2937035099090721
1106	22-23	0.2076497731788649
1106	24-25	0.23878019848316256
1106	26-27	0.3590734136106448
1106	28-29	0.40693389448091466
1106	30-31	0.38076643055773474
1106	32-33	0.3655159754823387
1106	34-35	0.29092664071836793
1106	36-37	0.31233133891543474
1106	38-39	0.2935983427406583
1106	40-41	0.28034347922987024
1106	42-43	0.26443105700737846
1106	44-45	0.1975040789770759
1106	46-47	0.2023323760611362
1106	48-49	0.1936569366329408
1106	50-51	0.20402815335695834
1106	52-53	0.2077266025739135
1106	54-55	0.17021864241273477
1106	56-57	0.08930751540670201
1106	58-59	0.1853961724148121
1106	60-61	0.13839297987004784
1106	62-63	0.19592995786747025
1106	64-65	0.18682182200940645
1106	66-67	0.2381662974612837
1106	68-69	0.2756001692126304
1106	70-71	0.3223478738102692
1106	72-73	0.24421311583918026
1106	74-75	0.2581031192610084
1106	76-77	0.3075897658414952
1106	78-79	0.2796284191191596
1106	80-81	0.1958654352050644
1106	82-83	0.17451859230424205
1106	84-85	0.17548260339971478
1106	86-87	0.23560004570416027
1106	88-89	0.2911357405051902
1106	90-91	0.2981914720225234
1106	92-93	0.2083014174640354
1106	94-95	0.3036234602114263
1106	96-97	0.315588535475122
1106	98-99	0.3548573334749179
1106	100	0.34241354108502264
1107	1	0.17666963874488317
1107	2	0.18780454526692125
1107	3	0.20447014014302312
1107	4	0.22029250816408563
1107	5	0.17097621723522138
1107	6	0.2645400065541992
1107	7	0.2934958212239067
1107	8	0.30474368665203855
1107	9	0.41752794805144333
1107	10-11	0.36873025957547156
1107	12-13	0.3545036147877667
1107	14-15	0.3741565098938011
1107	16-17	0.3715121875077898
1107	18-19	0.37642906622257755
1107	20-21	0.3314411870568321
1107	22-23	0.33442576689778747
1107	24-25	0.3751453857016003
1107	26-27	0.31866960508355646
1107	28-29	0.4174032888402195
1107	30-31	0.3218636897375333
1107	32-33	0.3491911052745138
1107	34-35	0.35392472368640426
1107	36-37	0.3125265772809911
1107	38-39	0.26408383377888356
1107	40-41	0.3501203765477072
1107	42-43	0.3112468022170347
1107	44-45	0.29941523596586705
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>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	159773.0
3	33039.0
4	23762.0
5	21013.0
6	13907.0
7	12888.0
8	13661.0
9	13638.0
10	13760.0
11	14049.0
12	15086.0
13	16463.0
14	16844.0
15	17354.0
16	17413.0
17	17133.0
18	16350.0
19	15463.0
20	14896.0
21	16504.0
22	20222.0
23	33052.0
24	51436.0
25	61551.0
26	67808.0
27	52915.0
28	51629.0
29	60527.0
30	70600.0
31	79634.0
32	99669.0
33	128702.0
34	174073.0
35	270685.0
36	406658.0
37	695730.0
38	890230.0
39	375111.0
40	1279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.43479717663693	22.31190773381052	13.14572279641078	21.10757229314177
2	15.90109042640665	24.024602705914752	37.90803856279512	22.16626830488348
3	17.267190940578224	30.318459705862356	29.212541043018557	23.201808310540862
4	11.991816637703357	21.295666303398033	37.328380116442325	29.384136942456283
5	11.630593288213689	41.0137364302954	32.57465469923469	14.781015582256213
6	26.570714844836353	41.20902142838383	18.517600317526632	13.70266340925319
7	24.508326370554663	36.856106610532436	21.52193250735633	17.113634511556576
8	23.682012476114856	37.83546893427766	20.6083916169737	17.874126972633793
9	24.233527194809305	21.40913034780933	21.055442582190683	33.301899875190685
10-11	21.711024469504242	31.344294846542496	27.480398256859228	19.46428242709403
12-13	22.676062163041454	29.763133889505028	26.99373364948371	20.56707029796981
14-15	20.920563351242823	31.482773262906242	24.93110286952744	22.66556051632349
16-17	19.681208846433034	34.06246107457789	25.548306180390608	20.70802389859847
18-19	19.59920211532217	32.63722141348898	27.622719439762626	20.140857031426222
20-21	21.91776837677995	31.79620800967633	26.52267693467692	19.7633466788668
22-23	21.263416230125546	32.076169358728194	26.72062407360546	19.939790337540803
24-25	20.613208334437825	31.87955356976307	27.136612738066834	20.37062535773227
26-27	20.44493353253569	31.654263153773858	27.184887325246937	20.715915988443516
28-29	20.42864487632487	31.74334230480663	27.322585320944853	20.50542749792364
30-31	20.976791231037442	32.45980454455868	26.520176944190617	20.04322728021326
32-33	20.156589227007746	31.973431938640605	27.368090223316184	20.50188861103547
34-35	20.420540745384116	31.22971738472013	27.434306538886904	20.915435331008844
36-37	20.967489760013194	31.895558516173878	26.75297185427213	20.3839798695408
38-39	20.729807671421362	31.780291422853992	27.100450229937938	20.389450675786705
40-41	20.42813355829001	31.4262249936553	27.387558887907666	20.75808256014703
42-43	20.26504571564896	31.602065230090155	27.379430068752647	20.753458985508242
44-45	20.773871468122287	31.671542735965758	27.0826051954682	20.471980600443754
46-47	20.519480551356413	32.20005532316065	26.853724728741017	20.426739396741922
48-49	20.261528383497076	31.538706598366318	27.340989019862523	20.858775998274083
50-51	20.330696383697834	31.327099115280348	27.322488799723832	21.01971570129799
52-53	20.3514137950881	31.484286959175655	27.12210389310447	21.042195352631772
54-55	20.577969008920917	31.97041259710407	26.94998148762432	20.501636906350697
56-57	20.25640182348948	31.64740650493105	26.858942672589198	21.237248998990278
58-59	20.172388741791423	31.07847150841499	27.449970498694775	21.299169251098814
60-61	20.705347934570305	31.732680338841114	26.764167508108027	20.797804218480557
62-63	20.409899356120683	31.570065134060123	26.998323174697607	21.021712335121588
64-65	20.420597534927587	31.19014265561943	27.316234785047083	21.073025024405897
66-67	20.224633749721278	31.27975386597005	26.968861777373682	21.526750606934993
68-69	20.90331631754584	31.588434827758565	26.875736236436037	20.63251261825956
70-71	20.924825712050094	31.216049316052946	26.982712579265307	20.876412392631654
72-73	20.503753565254996	31.24966099244722	27.51322712364762	20.733358318650165
74-75	20.526365070262553	31.298218030572134	27.219724442626042	20.955692456539268
76-77	20.650776931966405	31.252077764250195	26.825649916067224	21.27149538771618
78-79	20.868053535809455	31.319837706906885	27.11625417213072	20.695854585152944
80-81	20.494928997123953	31.148219761620403	27.306470590834053	21.05038065042159
82-83	20.355205774947006	31.22873473987876	27.458364084078575	20.957695401095663
84-85	20.52535910861836	30.980042676285112	27.2340622924538	21.260535922642728
86-87	20.962798394067928	31.091752540377783	27.02351564428968	20.92193342126461
88-89	20.57527352925945	31.014864175993335	27.217127709000554	21.19273458574666
90-91	20.44365539867087	30.812627426706403	27.13031927883387	21.613397895788854
92-93	20.65230249965762	31.07655413143242	26.945158524032266	21.325984844877695
94-95	20.61572674988667	30.804229607102457	27.206282806808623	21.373760836202248
96-97	20.594380484797433	30.920427425189796	27.118289022993075	21.366903067019695
98-99	20.487292641266063	30.91580528861641	27.14100780464902	21.455894265468512
100	20.59176378535616	31.070838475199757	26.93807867472872	21.399319064715357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	56019.0
1	41638.5
2	23201.0
3	15734.5
4	10228.5
5	7060.0
6	5496.0
7	4990.0
8	5075.5
9	5081.0
10	4374.0
11	3573.0
12	3289.5
13	3145.5
14	3211.5
15	3401.0
16	3606.5
17	4014.0
18	4716.5
19	5529.0
20	6644.5
21	8374.0
22	11326.0
23	16308.0
24	21778.0
25	28131.0
26	36876.5
27	47948.5
28	61566.5
29	73090.0
30	80531.0
31	88529.5
32	97190.5
33	105052.5
34	112654.0
35	119455.5
36	124036.0
37	127047.0
38	129195.0
39	129412.5
40	129175.5
41	127855.0
42	123931.0
43	122496.5
44	121288.0
45	118873.0
46	119289.5
47	119918.0
48	121971.0
49	124391.0
50	125718.5
51	120640.0
52	111465.0
53	105015.5
54	100780.5
55	98473.0
56	90834.0
57	80502.0
58	73440.5
59	67087.0
60	57809.5
61	47161.0
62	38184.5
63	29356.0
64	21841.0
65	16616.0
66	12516.0
67	9219.5
68	6843.5
69	5104.0
70	3738.0
71	2866.5
72	2250.0
73	1671.5
74	1285.5
75	1028.5
76	703.5
77	561.0
78	457.0
79	321.5
80	229.0
81	160.0
82	126.0
83	106.5
84	98.0
85	81.0
86	66.5
87	55.5
88	48.5
89	40.0
90	32.0
91	27.0
92	23.5
93	21.0
94	25.0
95	25.5
96	21.5
97	21.0
98	20.0
99	27.5
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0157565074744012
2	0.0078782537372006
3	0.010504338316267466
4	0.009498081608400722
5	0.01104428094000084
6	0.012713194504267633
7	0.011363337944934196
8	0.007902796583733933
9	0.007166511187733878
10-11	0.00618479732640047
12-13	0.007006982685267199
14-15	0.007780082351067258
16-17	0.005141726348733724
18-19	0.005743026088800437
20-21	0.005767568935333772
22-23	0.005988454554133788
24-25	0.00566939754920043
26-27	0.0063320544056004804
28-29	0.01640689290753458
30-31	0.005595769009600425
32-33	0.0053135262744670704
34-35	0.005031283539333716
36-37	0.0069456255689338605
38-39	0.006270697289267144
40-41	0.005914826014533783
42-43	0.004687683687867023
44-45	0.004589512301733682
46-47	0.006479311484800493
48-49	0.00368142698000028
50-51	0.004061841101266976
52-53	0.003350098551800255
54-55	0.0050435549626003835
56-57	0.002969684430533559
58-59	0.002724255965200207
60-61	0.0036568841334669445
62-63	0.003460541361200263
64-65	0.004528155185400344
66-67	0.0034728127844669303
68-69	0.003767326942866953
70-71	0.005276712004667067
72-73	0.0027978845048002125
74-75	0.0027119845419335395
76-77	0.002834698774600215
78-79	0.00368142698000028
80-81	0.004049569678000308
82-83	0.001963427722666816
84-85	0.0027733416582668776
86-87	0.0027733416582668776
88-89	0.001963427722666816
90-91	0.002601541732533531
92-93	0.0026751702721335368
94-95	0.002110684801866827
96-97	0.0015461993316001174
98-99	0.0018775277598001424
100	0.002724255965200207
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4074507.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.06887352683126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86953993701009	49.11144165879825
2	14.453312934774962	18.231083310540726
3	4.053118998172145	7.6687694845474805
4	1.4656008459822216	3.6973517758427854
5	0.7005829485857783	2.2092488689705485
6	0.38442043077009763	1.4546978117621567
7	0.2157200824740487	0.9523655819127371
8	0.1438674687002901	0.725884735046716
9	0.10170128154409648	0.5772766736899151
>10	0.5351108749332317	6.22810491420887
>50	0.044463343609362126	1.9435981299900027
>100	0.029711913141077267	3.6702389511587166
>500	0.0021464619203471792	0.9864894368217884
>1k	5.853986519405272E-4	0.5885609379044283
>5k	7.805315359206752E-5	0.3019353055065849
>10k+	3.902657679603376E-5	1.6529524232982973
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67156	1.6481994017926587	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6472	0.15884130276374542	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5795	0.14222579566067747	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.817138613334079E-5	0.0
5	0.0	0.0	0.0	9.817138613334079E-5	0.0
6	0.0	0.0	0.0	9.817138613334079E-5	0.0
7	0.0	0.0	0.0	1.22714232666676E-4	0.0
8	0.0	0.0	0.0	1.22714232666676E-4	0.0
9	0.0	0.0	0.0	1.22714232666676E-4	0.0
10-11	0.0	0.0	0.0	1.349856559333436E-4	0.0
12-13	0.0	0.0	0.0	1.472570792000112E-4	0.0
14-15	0.0	0.0	0.0	1.472570792000112E-4	0.0
16-17	0.0	0.0	0.0	1.84071349000014E-4	0.0
18-19	0.0	0.0	0.0	3.0678558166669E-4	0.0
20-21	0.0	0.0	0.0	4.540426608667012E-4	0.0
22-23	0.0	0.0	0.0	0.0011535137870667543	0.0
24-25	0.0	0.0	0.0	0.002822427351333548	0.0
26-27	0.0	0.0	0.0	0.005227626311600397	0.0
28-29	0.0	0.0	0.0	0.0096576101108674	0.0
30-31	0.0	0.0	0.0	0.01943793445440148	0.0
32-33	0.0	0.0	0.0	0.038311383438536246	0.0
34-35	0.0	0.0	0.0	0.06738238515727178	0.0
36-37	0.0	0.0	0.0	0.10987832392974167	0.0
38-39	0.0	0.0	0.0	0.17041325490421294	0.0
40-41	0.0	0.0	0.0	0.24377182319235186	0.0
42-43	0.0	0.0	0.0	0.32148674674015776	0.0
44-45	0.0	0.0	0.0	0.4104054797304312	0.0
46-47	0.0	1.2271423266667599E-5	0.0	0.5201488180042395	0.0
48-49	0.0	2.4542846533335197E-5	0.0	0.640371951747782	0.0
50-51	0.0	2.4542846533335197E-5	0.0	0.7663749258499248	0.0
52-53	0.0	2.4542846533335197E-5	0.0	0.8940958992094012	0.0
54-55	0.0	2.4542846533335197E-5	0.0	1.0382851225927454	0.0
56-57	0.0	2.4542846533335197E-5	0.0	1.2080111777940252	0.0
58-59	0.0	2.4542846533335197E-5	0.0	1.400316651805973	0.0
60-61	0.0	2.4542846533335197E-5	0.0	1.6021324788495885	0.0
62-63	0.0	2.4542846533335197E-5	0.0	1.8051018196802706	0.0
64-65	0.0	2.4542846533335197E-5	0.0	2.033055778281888	0.0
66-67	0.0	2.4542846533335197E-5	0.0	2.302106733403575	0.0
68-69	0.0	2.4542846533335197E-5	0.0	2.5881536097495967	0.0
70-71	0.0	2.4542846533335197E-5	0.0	2.908486842702688	0.0
72-73	0.0	2.4542846533335197E-5	0.0	3.2628364609509815	0.0
74-75	0.0	3.68142698000028E-5	0.0	3.6169038364641413	0.0
76-77	0.0	4.9085693066670394E-5	0.0	3.9892924469144364	0.0
78-79	0.0	4.9085693066670394E-5	0.0	4.406005438203935	0.0
80-81	0.0	4.9085693066670394E-5	0.0	4.86883443812957	0.0
82-83	0.0	4.9085693066670394E-5	0.0	5.3493342875592065	0.0
84-85	0.0	4.9085693066670394E-5	0.0	5.83021455111011	0.0
86-87	0.0	4.9085693066670394E-5	0.0	6.341515672939082	0.0
88	0.0	4.9085693066670394E-5	0.0	6.746754883474246	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	4575	0.0	45.720688	2
GGTATCA	7570	0.0	40.05441	1
GTACCTG	5550	0.0	38.200584	1
ACCTGGG	6325	0.0	32.327534	3
GTATCAA	11750	0.0	31.16636	1
TCTGTCG	1245	0.0	27.180933	8
GTCTCGC	995	0.0	26.45766	1
ATCAACG	13325	0.0	26.209904	3
TCAACGC	13300	0.0	26.047121	4
GTACATG	21785	0.0	25.527819	1
TACATGG	21405	0.0	25.100065	2
TATCAAC	14505	0.0	24.823034	2
CATGGGT	10510	0.0	24.598227	4
CAACGCA	14560	0.0	23.889893	5
ACATGGG	22400	0.0	23.103785	3
GTACACG	435	0.0	22.694286	1
AACGCAG	15475	0.0	22.385393	6
CTGTCGC	1435	0.0	22.27195	9
ATGGGTG	5715	0.0	20.973349	5
TATAACG	405	0.0	20.8911	2
>>END_MODULE