##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431459_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4074507
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.207421167763364	33.0	31.0	34.0	30.0	34.0
2	32.30745658309091	33.0	31.0	34.0	30.0	34.0
3	32.43056988244222	34.0	31.0	34.0	30.0	34.0
4	36.00411730793443	37.0	35.0	37.0	35.0	37.0
5	35.92245933066258	37.0	35.0	37.0	35.0	37.0
6	35.97395267697417	37.0	35.0	37.0	35.0	37.0
7	35.91690724791981	37.0	35.0	37.0	35.0	37.0
8	35.89955422827842	37.0	35.0	37.0	35.0	37.0
9	37.566542651663134	39.0	37.0	39.0	35.0	39.0
10-11	37.50588046602939	39.0	37.0	39.0	34.5	39.0
12-13	37.484776072295375	39.0	37.0	39.0	34.0	39.0
14-15	38.774289380285765	40.0	38.0	41.0	34.0	41.0
16-17	38.83200581076434	40.0	38.0	41.0	34.5	41.0
18-19	38.94217509013974	40.0	38.5	41.0	35.0	41.0
20-21	38.94146555644646	40.0	38.5	41.0	35.0	41.0
22-23	38.819369312655496	40.0	38.0	41.0	35.0	41.0
24-25	38.737741032227945	40.0	38.0	41.0	34.5	41.0
26-27	38.565364595029536	40.0	38.0	41.0	34.0	41.0
28-29	38.437241855272305	40.0	38.0	41.0	34.0	41.0
30-31	38.172915029965594	40.0	37.5	41.0	34.0	41.0
32-33	37.85608430664127	40.0	36.5	41.0	33.0	41.0
34-35	37.6279197704164	40.0	36.0	41.0	33.0	41.0
36-37	37.43409607591789	40.0	36.0	41.0	33.0	41.0
38-39	37.302867684360336	40.0	36.0	41.0	33.0	41.0
40-41	37.14110148786098	40.0	36.0	41.0	32.0	41.0
42-43	36.96044490781338	40.0	35.5	41.0	31.5	41.0
44-45	36.669485167162556	40.0	35.0	41.0	31.0	41.0
46-47	36.231514389348206	40.0	35.0	41.0	29.5	41.0
48-49	35.87405519244414	40.0	35.0	41.0	27.5	41.0
50-51	35.70062427184442	40.0	35.0	41.0	27.0	41.0
52-53	35.552702204217596	40.0	35.0	41.0	27.0	41.0
54-55	35.39107553380077	39.0	35.0	41.0	26.0	41.0
56-57	35.16549081888925	39.0	35.0	41.0	26.0	41.0
58-59	34.99421684635712	39.0	35.0	41.0	26.0	41.0
60-61	34.781305811966945	38.5	35.0	41.0	25.5	41.0
62-63	34.53876849395522	38.0	35.0	41.0	25.0	41.0
64-65	34.28725818853667	37.5	34.5	40.0	24.0	41.0
66-67	34.02667512904015	37.0	34.0	40.0	23.0	41.0
68-69	33.72994205188505	36.5	34.0	40.0	23.0	41.0
70-71	33.39902447093599	36.0	34.0	39.0	22.5	41.0
72-73	33.07654889290901	35.5	34.0	39.0	22.0	41.0
74-75	32.77242375580653	35.0	33.5	39.0	20.5	40.5
76-77	32.431422255502326	35.0	33.0	37.5	20.0	40.0
78-79	32.12290812115429	35.0	33.0	37.0	19.5	39.0
80-81	31.8263572746347	35.0	33.0	36.5	18.5	39.0
82-83	31.57832567228379	35.0	33.0	36.0	18.0	38.5
84-85	31.347539101049527	35.0	33.0	36.0	16.5	37.0
86-87	31.10120709082105	35.0	33.0	35.5	12.5	37.0
88-89	30.942812345149978	35.0	33.0	35.0	8.5	36.5
90-91	30.751605899805792	35.0	33.0	35.0	4.5	36.0
92-93	30.550484635319073	35.0	33.0	35.0	2.0	36.0
94-95	30.38089896519996	35.0	33.0	35.0	2.0	36.0
96-97	30.20688343399582	35.0	32.0	35.0	2.0	36.0
98-99	30.035534237639055	35.0	32.0	35.0	2.0	35.0
100	29.91982711037188	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18383469162326094
1101	2	0.3111896997636947
1101	3	0.22715320796265814
1101	4	0.22077124132308512
1101	5	0.1903778210152538
1101	6	0.1804678919817917
1101	7	0.2536851960264954
1101	8	0.25015817716568733
1101	9	0.35105275053263796
1101	10-11	0.3075927685081581
1101	12-13	0.34201870511596155
1101	14-15	0.4592330909469382
1101	16-17	0.44850422673928136
1101	18-19	0.4547390133385534
1101	20-21	0.40496248562410386
1101	22-23	0.41670556382048574
1101	24-25	0.44574220744109994
1101	26-27	0.43878259119873775
1101	28-29	0.46728090221590435
1101	30-31	0.4230955377033183
1101	32-33	0.4339220179913639
1101	34-35	0.5327982955894655
1101	36-37	0.5157466080449353
1101	38-39	0.5235368999268815
1101	40-41	0.5902299950891816
1101	42-43	0.6393269155695265
1101	44-45	0.63124682256651
1101	46-47	0.6395292264342416
1101	48-49	0.5684812429319663
1101	50-51	0.46666719510029253
1101	52-53	0.5268126058459117
1101	54-55	0.4807849681142926
1101	56-57	0.4993356158047533
1101	58-59	0.5042194536224827
1101	60-61	0.5516772965215182
1101	62-63	0.497850501158247
1101	64-65	0.4537966866283156
1101	66-67	0.4494953010443581
1101	68-69	0.36416693518378196
1101	70-71	0.4467884594802811
1101	72-73	0.47549062487022553
1101	74-75	0.34377836291557173
1101	76-77	0.3572572686738198
1101	78-79	0.39674860872954554
1101	80-81	0.3685262623808896
1101	82-83	0.3257290048095811
1101	84-85	0.409645923477985
1101	86-87	0.3746468428119627
1101	88-89	0.30778142548723153
1101	90-91	0.33463896830923545
1101	92-93	0.3984526656883993
1101	94-95	0.39942872049183364
1101	96-97	0.3741186836064756
1101	98-99	0.4312966841816923
1101	100	0.43265001865577446
1102	1	0.12096633732870288
1102	2	0.2005109698531342
1102	3	0.1928711906776286
1102	4	0.1627948619823485
1102	5	0.09898648601114957
1102	6	0.0902772610501259
1102	7	0.15823407617834562
1102	8	0.1638785204137747
1102	9	0.23718781185795734
1102	10-11	0.25503648231140374
1102	12-13	0.27654795247888586
1102	14-15	0.35594054323172486
1102	16-17	0.3178078439241716
1102	18-19	0.2996858374762823
1102	20-21	0.21204344628955596
1102	22-23	0.2424284770862215
1102	24-25	0.25021023166978296
1102	26-27	0.22625541568901042
1102	28-29	0.23458426137715094
1102	30-31	0.19490057912761216
1102	32-33	0.22063392820935235
1102	34-35	0.20031873949231027
1102	36-37	0.1752243784141143
1102	38-39	0.22414427735926523
1102	40-41	0.18397253858915974
1102	42-43	0.1880868054113165
1102	44-45	0.15242149435277952
1102	46-47	0.08955745575339336
1102	48-49	0.05705456379841678
1102	50-51	0.00506832989338335
1102	52-53	0.001236395625625164
1102	54-55	0.02448452896316411
1102	56-57	0.08723750039921185
1102	58-59	-0.006547540810849739
1102	60-61	0.011302153139567395
1102	62-63	0.010499982428541443
1102	64-65	0.04950391410042698
1102	66-67	0.008525617544840713
1102	68-69	0.03758966987672352
1102	70-71	-0.01941721346326375
1102	72-73	-0.052637528574642545
1102	74-75	-0.009641791540957456
1102	76-77	0.03056919370901312
1102	78-79	0.023587601977503425
1102	80-81	-0.041935574389775354
1102	82-83	-0.00979752947307233
1102	84-85	-0.04765295220315835
1102	86-87	-0.012789321883762028
1102	88-89	0.022555258824393576
1102	90-91	-0.04709940849214078
1102	92-93	-0.01294527318810168
1102	94-95	0.04004981660691698
1102	96-97	0.013081466889609317
1102	98-99	0.10590881844595401
1102	100	0.08029676640828853
1103	1	0.14237446416905186
1103	2	0.20775590404409883
1103	3	0.17917319647659014
1103	4	0.15438903333006238
1103	5	0.09665507615815017
1103	6	0.11179529690075896
1103	7	0.1265935761589816
1103	8	0.142829170209545
1103	9	0.24409707209995446
1103	10-11	0.22840702363289012
1103	12-13	0.24516267176753814
1103	14-15	0.36670392015461317
1103	16-17	0.3944572979472696
1103	18-19	0.37666777403941865
1103	20-21	0.36632663155603495
1103	22-23	0.28592761444060244
1103	24-25	0.34522379998379904
1103	26-27	0.322210978946849
1103	28-29	0.2598106210076452
1103	30-31	0.3280016982815539
1103	32-33	0.32321683354101083
1103	34-35	0.32010301207960623
1103	36-37	0.3919340445161481
1103	38-39	0.3501118502781324
1103	40-41	0.34737163321290865
1103	42-43	0.35036183910325747
1103	44-45	0.40157058689043623
1103	46-47	0.3600257671487199
1103	48-49	0.3638049759416333
1103	50-51	0.31809468663448826
1103	52-53	0.3227139145937912
1103	54-55	0.2762107542742669
1103	56-57	0.3077494111110042
1103	58-59	0.3648875619973566
1103	60-61	0.2823190132440203
1103	62-63	0.28777662770414736
1103	64-65	0.30199464531479236
1103	66-67	0.3422513592563021
1103	68-69	0.296190236745673
1103	70-71	0.3089755126650289
1103	72-73	0.2571103326831903
1103	74-75	0.24472176741367946
1103	76-77	0.2700848549023078
1103	78-79	0.2843885129830923
1103	80-81	0.2290714779701304
1103	82-83	0.26579861326242593
1103	84-85	0.2522346852936366
1103	86-87	0.27088394995117326
1103	88-89	0.2769242353903003
1103	90-91	0.28255387746346017
1103	92-93	0.29208401989831856
1103	94-95	0.3270053824801522
1103	96-97	0.2911549680576364
1103	98-99	0.3400204279634629
1103	100	0.3648581995669815
1104	1	0.06099883560043651
1104	2	0.1749754991547121
1104	3	0.1364988041656332
1104	4	0.12541063646599326
1104	5	0.11760705479428424
1104	6	0.09323640273693456
1104	7	0.04224532625513433
1104	8	0.09171734146656974
1104	9	0.16375212635698233
1104	10-11	0.19473636813355455
1104	12-13	0.19727338888494472
1104	14-15	0.29892377342147824
1104	16-17	0.25291678821299257
1104	18-19	0.2672045071629938
1104	20-21	0.2229060033662904
1104	22-23	0.23529991829146013
1104	24-25	0.2594433655737731
1104	26-27	0.24223958289763914
1104	28-29	0.28599311703430885
1104	30-31	0.23415878145543445
1104	32-33	0.20982457206125105
1104	34-35	0.20555729416263802
1104	36-37	0.2735757712180842
1104	38-39	0.27418191412160553
1104	40-41	0.24313635893504681
1104	42-43	0.27564304116955896
1104	44-45	0.252714873402752
1104	46-47	0.2573133906125378
1104	48-49	0.2003216785310613
1104	50-51	0.22321815882161644
1104	52-53	0.2716130216361279
1104	54-55	0.3058257871425738
1104	56-57	0.2711050287462342
1104	58-59	0.2849044533037741
1104	60-61	0.2639140566919522
1104	62-63	0.28262212298527345
1104	64-65	0.21532413956998653
1104	66-67	0.275646017645073
1104	68-69	0.2939322809319833
1104	70-71	0.26184920996201555
1104	72-73	0.2700293943665244
1104	74-75	0.2611669625164552
1104	76-77	0.2740659438818369
1104	78-79	0.2932617075924995
1104	80-81	0.26908430630363256
1104	82-83	0.26166182379864367
1104	84-85	0.2806230427578029
1104	86-87	0.233390266412961
1104	88-89	0.1934543142976679
1104	90-91	0.18662561693217938
1104	92-93	0.20728109828674235
1104	94-95	0.1826044776664233
1104	96-97	0.20626348451258636
1104	98-99	0.24443495649486735
1104	100	0.33047337155282364
1105	1	0.09123463063301784
1105	2	0.16512438246104466
1105	3	0.12439336937628553
1105	4	0.12994882939354113
1105	5	0.055615186741981404
1105	6	0.11837807993880745
1105	7	0.11806277375146124
1105	8	0.10926130180292404
1105	9	0.17376942077812174
1105	10-11	0.1853879742179103
1105	12-13	0.1293394259797367
1105	14-15	0.2520077921458679
1105	16-17	0.20951483025999806
1105	18-19	0.16730067273029903
1105	20-21	0.16966836709251254
1105	22-23	0.1943931945299795
1105	24-25	0.17104560984257944
1105	26-27	0.19145580138470564
1105	28-29	0.21604246645324565
1105	30-31	0.2144205778071111
1105	32-33	0.22564632164096565
1105	34-35	0.27552606617953046
1105	36-37	0.2335207979844327
1105	38-39	0.25729287940673373
1105	40-41	0.30549008178675763
1105	42-43	0.3779221610973593
1105	44-45	0.502211045834116
1105	46-47	0.5062258318520279
1105	48-49	0.4938491091335635
1105	50-51	0.41204769542479625
1105	52-53	0.4078446100851494
1105	54-55	0.46137207138448844
1105	56-57	0.4472386701889235
1105	58-59	0.38414458118171524
1105	60-61	0.4579884935242191
1105	62-63	0.4017945820070068
1105	64-65	0.4174718841097871
1105	66-67	0.4443424525172972
1105	68-69	0.4213055003251469
1105	70-71	0.4029250613854103
1105	72-73	0.39737810749492297
1105	74-75	0.4283364332660753
1105	76-77	0.4694376488222787
1105	78-79	0.4344984567050503
1105	80-81	0.35239529034437567
1105	82-83	0.36807309022960766
1105	84-85	0.3903425114528005
1105	86-87	0.36724430239389605
1105	88-89	0.356784379363809
1105	90-91	0.36428332421557386
1105	92-93	0.3875525393170953
1105	94-95	0.35887954665701116
1105	96-97	0.2974652705888481
1105	98-99	0.36256624361439904
1105	100	0.3794372042086671
1106	1	0.09581770472043871
1106	2	0.21073358970049583
1106	3	0.17597225943844563
1106	4	0.166910823790559
1106	5	0.17111889330016794
1106	6	0.12194762101547241
1106	7	0.15606183495001602
1106	8	0.14906035150345787
1106	9	0.16398244066908063
1106	10-11	0.19498464662304826
1106	12-13	0.20462522848450249
1106	14-15	0.24307297642209846
1106	16-17	0.2032537747143195
1106	18-19	0.2187943255643674
1106	20-21	0.19225383480761593
1106	22-23	0.17816293138339034
1106	24-25	0.2232949496074852
1106	26-27	0.22340335202158457
1106	28-29	0.15064860365563248
1106	30-31	0.1869259031242052
1106	32-33	0.1522534508660982
1106	34-35	0.24257242519718858
1106	36-37	0.19986507943681886
1106	38-39	0.16934585588241902
1106	40-41	0.1736841940370013
1106	42-43	0.1797681416672674
1106	44-45	0.12073977310841144
1106	46-47	0.15319112596925066
1106	48-49	0.13634457686917756
1106	50-51	0.05953656787791317
1106	52-53	0.045653610282684554
1106	54-55	0.11721169744280502
1106	56-57	0.09161318222312786
1106	58-59	0.12516659245542172
1106	60-61	0.10408874758382325
1106	62-63	0.09499563935888489
1106	64-65	0.0917408522204255
1106	66-67	0.05960760571451118
1106	68-69	0.11818667153511342
1106	70-71	0.09436590760841312
1106	72-73	0.15033698966592368
1106	74-75	0.14891901461302126
1106	76-77	0.1828528049050817
1106	78-79	0.12410801919663328
1106	80-81	0.15429493571869912
1106	82-83	0.11129377847739619
1106	84-85	0.06659147800338516
1106	86-87	0.08913162315802481
1106	88-89	0.0569054384032448
1106	90-91	0.11276770928205693
1106	92-93	0.08472558219965265
1106	94-95	0.12806108299276886
1106	96-97	0.10568165983120892
1106	98-99	0.1043519111532838
1106	100	0.1949628590065977
1107	1	0.08947911140904807
1107	2	0.06800848826740236
1107	3	0.06997140209959696
1107	4	0.06968367225412209
1107	5	0.08071732541106513
1107	6	0.02440375154839103
1107	7	0.0034286645346526257
1107	8	0.05272776663969836
1107	9	0.08012214198131318
1107	10-11	0.06902213045949424
1107	12-13	-5.876077338200503E-4
1107	14-15	0.11646351624486329
1107	16-17	0.12747600129631564
1107	18-19	0.06714692865234184
1107	20-21	0.0239855810188061
1107	22-23	0.00894414857620518
1107	24-25	0.028292628295815803
1107	26-27	0.11510924459392413
1107	28-29	0.11631323879266375
1107	30-31	0.10537104938507014
1107	32-33	0.08114070716254673
1107	34-35	0.13663732629472491
1107	36-37	0.20129307071934477
1107	38-39	0.16163631911073395
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2216	66-67	-1.1947252233849994
2216	68-69	-1.055972283831494
2216	70-71	-1.1195007312384533
2216	72-73	-0.9803790223661437
2216	74-75	-0.9736255231436601
2216	76-77	-0.9021200186304625
2216	78-79	-0.8933300556733919
2216	80-81	-0.9727227472399349
2216	82-83	-1.0840878222041894
2216	84-85	-1.0461005394535405
2216	86-87	-1.0430801898233923
2216	88-89	-1.0470097459898504
2216	90-91	-1.1165819334074207
2216	92-93	-1.062844636707375
2216	94-95	-1.1864009342902833
2216	96-97	-1.3711450402192185
2216	98-99	-1.452271321854905
2216	100	-1.4415559802123639
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	381.0
3	995.0
4	682.0
5	602.0
6	1170.0
7	2469.0
8	4108.0
9	5947.0
10	7864.0
11	10522.0
12	14660.0
13	20424.0
14	20681.0
15	24940.0
16	32029.0
17	45816.0
18	50836.0
19	28863.0
20	19092.0
21	16653.0
22	16520.0
23	16984.0
24	18716.0
25	22157.0
26	26774.0
27	33347.0
28	41746.0
29	52110.0
30	65002.0
31	80993.0
32	101030.0
33	127573.0
34	174771.0
35	276002.0
36	392543.0
37	726766.0
38	1065704.0
39	523479.0
40	3556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.96650770690081	20.771861346748782	14.407593924055357	22.854037022295046
2	14.552533594861906	22.085076795793945	39.66818562343861	23.694203985905535
3	16.019928825622777	28.68808442753712	31.745612958645232	23.54637378819487
4	10.865824660056163	18.909070457513835	38.12597287326037	32.09913200916963
5	10.659916417933893	37.84382126190904	35.04833568505559	16.447926635101474
6	26.339505613808	39.16213667076778	19.257544532381463	15.240813183042759
7	23.502131668935654	34.54857237943143	23.850615546862482	18.09868040477044
8	25.049509057169374	33.027676722607175	22.239696728953955	19.6831174912695
9	25.14885849993631	15.420221391201439	23.882447618816215	35.54847249004604
10-11	22.360615643517136	26.90685732877419	29.766727577117745	20.96579945059092
12-13	24.55314225745593	25.448428484722204	30.09513789030182	19.903291367520044
14-15	21.843072057551993	26.430780460065474	29.909311727774675	21.816835754607858
16-17	18.56765738775268	29.374559916083097	30.493456018114586	21.56432667804964
18-19	18.34096738574753	26.151458323669587	35.655479300931376	19.85209498965151
20-21	20.243921534556204	25.425174137631867	35.51791664611203	18.812987681699898
22-23	19.61995647571596	25.784984539233825	35.68051300439538	18.914545980654836
24-25	19.10849504513581	25.622711211562898	35.97890136191579	19.289892381385506
26-27	18.954685806160107	25.447360870898	36.040286591727536	19.557666731214354
28-29	18.988917024635583	25.532652226013425	36.06408650893332	19.414344240417673
30-31	19.634124850774402	26.197228625396622	35.33427761066256	18.83436891316641
32-33	18.745856087144915	25.772962845384363	36.05207558570676	19.429105481763962
34-35	18.965581984844036	25.279449720157587	35.83448114045723	19.920487154541146
36-37	19.717392923704754	25.97944776215197	34.863724871909	19.439434442234276
38-39	19.563433208547607	25.89701041931784	35.07456587402818	19.46499049810637
40-41	19.258870659468165	25.71560753793603	35.158668362597965	19.86685343999784
42-43	19.19121772496499	25.93039916709904	34.9038173154848	19.97456579245117
44-45	19.812261949727905	26.065042961025714	34.295817874407874	19.826877214838508
46-47	19.698514045333336	26.77413219093947	33.47436547195535	20.052988291771847
48-49	19.760328110017287	26.381087975001517	33.27976012685769	20.57882378812351
50-51	19.842241051785226	26.31471925985484	32.950986686772474	20.892053001587456
52-53	19.874076827443872	26.770522339294068	32.50882906568059	20.846571767581466
54-55	20.134866802741808	27.282174136012227	32.27026520908453	20.31269385216143
56-57	19.78747021692936	26.778519495293924	32.30678510966122	21.127225178115495
58-59	19.58956691548428	26.140699051369303	32.97808823491511	21.291645798231308
60-61	20.125483928446084	26.747762774312022	32.25730684708131	20.869446450160588
62-63	19.850112992027665	26.523447303009462	32.607848635474774	21.018591069488103
64-65	19.72939229478073	26.264848903866746	32.958473750748254	21.047285050604263
66-67	19.736907660594515	26.447459455771394	32.440352818664174	21.37528006496992
68-69	20.3701456198985	26.727427013647763	32.13828030710687	20.764147059346875
70-71	20.45314901567375	26.361634438687076	32.08390012240803	21.101316423231143
72-73	20.085325660257794	26.470674857105415	32.45899442558326	20.985005057053527
74-75	20.072291123015784	26.49902227207159	32.1451540956891	21.28353250922353
76-77	20.180735976156132	26.585915793002684	31.71181200572241	21.52153622511877
78-79	20.374771290113966	26.726739698391334	31.859228835055227	21.039260176439477
80-81	19.930926612716583	26.55954450440262	32.0164255454709	21.4931033374099
82-83	19.813199808907218	26.65606894458788	32.13189819854678	21.398833047958128
84-85	19.920954349768717	26.55606934969141	31.796479109854726	21.726497190685144
86-87	20.1660593539292	26.67049044215656	31.552909345842334	21.610540858071907
88-89	19.749739048184235	26.610605896615226	31.729261969607613	21.910393085592933
90-91	19.562452586288355	26.495193160792212	31.572371823143264	22.369982429776165
92-93	19.74444959436122	26.80196672008427	31.35831775166496	22.09526593388955
94-95	19.60514486783309	26.511685953662617	31.636085052743805	22.24708412576049
96-97	19.522315793555563	26.649979101684114	31.505775813469704	22.32192929129062
98-99	19.43879512581165	26.586731790667457	31.407134180320035	22.56733890320086
100	19.601982674667003	26.765371717139985	31.105417700741395	22.52722790745162
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5181.0
1	3374.5
2	13968.5
3	23029.0
4	12494.0
5	5308.0
6	8704.5
7	19748.5
8	30490.5
9	31788.5
10	20854.5
11	9438.5
12	7085.0
13	7091.0
14	7534.0
15	8059.0
16	8513.5
17	9091.0
18	9813.0
19	10687.0
20	11724.0
21	13131.5
22	15544.0
23	19677.0
24	24747.5
25	30698.0
26	38806.0
27	48924.0
28	62010.5
29	73249.5
30	79952.0
31	87049.5
32	95294.0
33	103500.0
34	111026.5
35	117380.5
36	121114.0
37	123665.5
38	126134.5
39	126370.5
40	126578.0
41	125838.5
42	121948.0
43	120349.0
44	119881.5
45	118333.5
46	118338.5
47	119237.0
48	120608.0
49	122330.5
50	125075.5
51	120627.5
52	109794.5
53	101585.0
54	96602.5
55	93702.0
56	85583.5
57	76125.5
58	70273.0
59	64717.0
60	55751.5
61	44747.0
62	35986.0
63	27379.5
64	20005.0
65	14892.5
66	10948.0
67	7934.0
68	5711.0
69	4028.0
70	2728.5
71	1955.0
72	1465.5
73	1091.5
74	854.0
75	663.0
76	424.5
77	319.5
78	221.0
79	100.5
80	54.0
81	21.0
82	7.0
83	2.5
84	4.5
85	4.0
86	1.0
87	1.5
88	0.5
89	0.0
90	0.0
91	1.0
92	2.5
93	3.0
94	2.5
95	2.0
96	2.5
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04879117890827037
2	0.0
3	1.7179992573334638E-4
4	0.06884268452600523
5	0.004614055148267017
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.012111894764200921
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.381140098667151E-4
26-27	0.0
28-29	3.5587127473336036E-4
30-31	0.003411455668133593
32-33	1.595285024666788E-4
34-35	0.001742542103866799
36-37	0.0038654983290002942
38-39	0.001374399405866771
40-41	0.0012148709034000922
42-43	0.0017916277969334696
44-45	0.0
46-47	5.399426237333744E-4
48-49	4.90856930666704E-4
50-51	0.0015461993316001174
52-53	9.448995915334052E-4
54-55	0.001043070977666746
56-57	0.006246154442733807
58-59	0.009117667487134025
60-61	0.0010062567078667432
62-63	0.0025647274627335283
64-65	0.0011780566336000895
66-67	1.472570792000112E-4
68-69	7.240139727333883E-4
70-71	4.785855074000364E-4
72-73	0.0
74-75	2.3315704206668437E-4
76-77	0.0
78-79	5.399426237333744E-4
80-81	0.0
82-83	0.0011412423638000868
84-85	6.381140098667151E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.8407134900001398E-4
94-95	0.0
96-97	3.9268554453336316E-4
98-99	5.767568935333772E-4
100	2.699713118666872E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4074507.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.45167360311436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30370854219316	41.94568702124828
2	16.344875133957483	18.45391112291656
3	4.881706240967305	8.267414620241178
4	1.8345515110228865	4.142540124334569
5	0.8188141778229518	2.3111715354031857
6	0.46295514055739573	1.568075549257802
7	0.2799794454991699	1.106371579103006
8	0.1774434971989004	0.801358590949397
9	0.1256000667846789	0.6381300577192253
>10	0.6680422656370459	6.91379923732011
>50	0.058591680309932954	2.3003278563799294
>100	0.03920829156350148	4.368432830254862
>500	0.003045004426810876	1.2018694323817858
>1k	0.0011310015744657664	1.0114581811063914
>5k	8.700012111275058E-5	0.3922411694096071
>10k+	2.610003633382517E-4	4.577211091974189
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48173	1.1823025460503565	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41807	1.0260627850191446	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37149	0.9117422058668693	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31696	0.7779100637205925	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17265	0.4237322453980322	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10305	0.2529140335260192	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8882	0.21798956290908322	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7091	0.1740333247678799	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.4542846533335197E-5	0.0
14-15	0.0	0.0	0.0	4.9085693066670394E-5	0.0
16-17	0.0	0.0	0.0	7.36285396000056E-5	0.0
18-19	0.0	0.0	0.0	1.84071349000014E-4	0.0
20-21	0.0	0.0	0.0	3.804141212666956E-4	0.0
22-23	0.0	0.0	0.0	0.0011166995172667514	0.0
24-25	0.0	0.0	0.0	0.0027487988117335426	0.0
26-27	0.0	0.0	0.0	0.005203083465067062	0.0
28-29	0.0	0.0	0.0	0.00975578149700074	0.0
30-31	0.0	0.0	0.0	0.019842891422201508	0.0
32-33	0.0	0.0	0.0	0.039293097299869656	0.0
34-35	0.0	0.0	0.0	0.06908811299133859	0.0
36-37	0.0	0.0	0.0	0.11252895135534188	0.0
38-39	0.0	0.0	0.0	0.17442601031241325	0.0
40-41	0.0	0.0	0.0	0.249968891942019	0.0
42-43	0.0	0.0	0.0	0.3300767430268251	0.0
44-45	0.0	0.0	0.0	0.42184244621496536	0.0
46-47	0.0	0.0	0.0	0.5340768834119073	0.0
48-49	0.0	0.0	0.0	0.6569015588879832	0.0
50-51	0.0	0.0	0.0	0.7859110316904597	0.0
52-53	0.0	0.0	0.0	0.916478975247803	0.0
54-55	0.0	0.0	0.0	1.0637237830245474	0.0
56-57	0.0	0.0	0.0	1.2376712078295609	0.0
58-59	0.0	0.0	0.0	1.4347011797991756	0.0
60-61	0.0	0.0	0.0	1.6415360189588581	0.0
62-63	0.0	0.0	0.0	1.8498802431803405	0.0
64-65	0.0	0.0	0.0	2.0835281421776917	0.0
66-67	0.0	0.0	0.0	2.358088966346113	0.0
68-69	0.0	0.0	0.0	2.6492530261943346	0.0
70-71	0.0	0.0	0.0	2.9754642708921595	0.0
72-73	0.0	0.0	0.0	3.336133672122787	0.0
74-75	0.0	0.0	0.0	3.697146673204881	0.0
76-77	0.0	0.0	0.0	4.076738609112709	0.0
78-79	0.0	0.0	0.0	4.502311568000742	0.0
80-81	0.0	0.0	0.0	4.974798178037245	0.0
82-83	0.0	0.0	0.0	5.466428208369749	0.0
84-85	0.0	0.0	0.0	5.958352752860653	0.0
86-87	0.0	0.0	0.0	6.483373325901759	0.0
88	0.0	0.0	0.0	6.8993132175254575	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15545	0.0	56.757755	1
TACCTGG	4490	0.0	42.95181	2
GTATCAA	28820	0.0	42.223007	1
TATAACG	480	0.0	40.177837	2
ATAACGC	545	0.0	36.249054	3
GTACCTG	5515	0.0	35.323967	1
ACCTGGG	5925	0.0	32.23158	3
ATCAACG	38570	0.0	31.451807	3
TATCAAC	38670	0.0	31.346148	2
TCAACGC	38945	0.0	31.221428	4
GTGGTAT	5830	0.0	30.671124	1
CAACGCA	40710	0.0	29.859192	5
TGGTATC	5990	0.0	29.60455	2
AACGCAG	42270	0.0	28.878126	6
TCTGTCG	1075	0.0	26.234459	8
CGCAGAG	46250	0.0	26.088161	8
ACGCAGA	46590	0.0	26.039019	7
GCAGAGT	48365	0.0	24.60718	9
CATGGGT	10030	0.0	24.245634	4
GTATAAC	1205	0.0	24.211367	1
>>END_MODULE