FastQCFastQC Report
Wed 25 May 2016
SRR2431458_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431458_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5449301
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA595281.0923969881641702No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100340.1841337081581656No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67610.1240709588257283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG52250.042.011382
GGTATCA128600.038.8193131
GTACCTG61350.036.3185121
GTATCAA210600.034.663891
ACCTGGG69900.029.9239713
ATCAACG256100.027.6225193
TCAACGC259450.027.2837244
TATCAAC270800.026.6437952
GTACATG403400.025.7642271
CAACGCA278450.025.3882645
TACATGG400850.025.164262
CATGGGT150900.024.6388034
AACGCAG288150.024.4178646
GTACACG6900.023.8442251
CGCAGAG298300.022.908878
TACACGG6800.022.8108462
ACATGGG421550.022.80243
ACGCAGA305550.022.7039057
TATAACG5550.022.019972
ATGGGTG85000.021.566425
Produced by FastQC (version 0.11.5)