FastQCFastQC Report
Wed 25 May 2016
SRR2431458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5449301
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT637831.1704803973940878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491200.9014000144238683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458070.8406032259917373No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT431250.7913859043572745No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164530.30192863268151277No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117040.21477984057037772No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109720.20134692504598295No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97810.17949091085260294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA229200.052.455021
TACCTGG50250.042.7719962
GTATCAA423950.041.9887051
TATAACG7250.038.2730832
GTACCTG63100.034.7510031
ACCTGGG62750.033.352293
ATAACGC9200.033.2280583
TAACGCA9100.032.0427444
TATCAAC587750.030.1187132
ATCAACG590450.030.0287783
TCAACGC598600.029.6435034
CAACGCA622500.028.604265
AACGCAG640900.027.9067066
ACGCAGA697650.025.5355857
CGCAGAG696150.025.502838
CATGGGT140650.024.4765824
TACACGG5200.024.4196722
GCAGAGT723700.024.2591869
GTATAAC16250.023.1658041
AGTACTT510800.022.8997412-13
Produced by FastQC (version 0.11.5)