##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431458_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5449301 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.236518408507806 33.0 31.0 34.0 30.0 34.0 2 32.32964503153707 34.0 31.0 34.0 30.0 34.0 3 32.43228406725927 34.0 31.0 34.0 30.0 34.0 4 36.00919475727254 37.0 35.0 37.0 35.0 37.0 5 35.946673343975675 37.0 35.0 37.0 35.0 37.0 6 36.00592699871048 37.0 35.0 37.0 35.0 37.0 7 35.942558871312116 37.0 35.0 37.0 35.0 37.0 8 35.92554090882482 37.0 35.0 37.0 35.0 37.0 9 37.595669793245044 39.0 37.0 39.0 35.0 39.0 10-11 37.526595154130774 39.0 37.0 39.0 34.5 39.0 12-13 37.50427274984443 39.0 37.0 39.0 34.5 39.0 14-15 38.76605237992909 40.0 38.0 41.0 34.0 41.0 16-17 38.84865939686576 40.0 38.0 41.0 35.0 41.0 18-19 38.966978975835616 40.0 38.5 41.0 35.0 41.0 20-21 38.965161311515004 40.0 38.5 41.0 35.0 41.0 22-23 38.85524060792384 40.0 38.0 41.0 35.0 41.0 24-25 38.778316154677455 40.0 38.0 41.0 35.0 41.0 26-27 38.61313276693653 40.0 38.0 41.0 34.0 41.0 28-29 38.49023204994549 40.0 38.0 41.0 34.0 41.0 30-31 38.2121737265018 40.0 37.5 41.0 34.0 41.0 32-33 37.89924625195048 40.0 36.5 41.0 33.5 41.0 34-35 37.68611010843409 40.0 36.0 41.0 33.0 41.0 36-37 37.49981841707771 40.0 36.0 41.0 33.0 41.0 38-39 37.36932112944395 40.0 36.0 41.0 33.0 41.0 40-41 37.20850353100333 40.0 36.0 41.0 33.0 41.0 42-43 37.01380030209379 40.0 35.5 41.0 31.5 41.0 44-45 36.68552260188967 40.0 35.0 41.0 31.0 41.0 46-47 36.195836585279466 40.0 35.0 41.0 29.5 41.0 48-49 35.817572657483964 40.0 35.0 41.0 27.0 41.0 50-51 35.63862750470198 40.0 35.0 41.0 27.0 41.0 52-53 35.49070064215576 40.0 35.0 41.0 26.0 41.0 54-55 35.3299148826611 39.0 35.0 41.0 26.0 41.0 56-57 35.09993887289397 39.0 35.0 41.0 25.0 41.0 58-59 34.922320128765136 39.0 35.0 41.0 25.0 41.0 60-61 34.70897928009482 38.5 35.0 41.0 25.0 41.0 62-63 34.472566481462486 38.0 35.0 41.0 24.5 41.0 64-65 34.22555700263208 37.5 34.5 40.0 23.5 41.0 66-67 33.955908565153585 37.0 34.0 40.0 23.0 41.0 68-69 33.661477774855896 36.5 34.0 40.0 22.5 41.0 70-71 33.329398394399576 36.0 34.0 39.0 22.0 41.0 72-73 33.0113838453776 35.5 34.0 39.0 21.5 41.0 74-75 32.7115641070295 35.0 33.5 38.5 20.0 40.5 76-77 32.372929665658035 35.0 33.0 37.0 20.0 40.0 78-79 32.07248076404662 35.0 33.0 37.0 18.5 39.0 80-81 31.782840037648867 35.0 33.0 36.5 18.0 39.0 82-83 31.540776330762423 35.0 33.0 36.0 18.0 38.5 84-85 31.31429719151135 35.0 33.0 36.0 14.5 37.0 86-87 31.07699730662703 35.0 33.0 35.5 11.0 37.0 88-89 30.92843467446559 35.0 33.0 35.0 7.5 36.5 90-91 30.736670171091667 35.0 33.0 35.0 2.0 36.0 92-93 30.543227837845624 35.0 33.0 35.0 2.0 36.0 94-95 30.379061002502887 35.0 33.0 35.0 2.0 36.0 96-97 30.20691240949986 35.0 32.5 35.0 2.0 36.0 98-99 30.043788918982454 35.0 32.0 35.0 2.0 35.0 100 29.929205782539814 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14298866885153672 1101 2 0.21046114886519973 1101 3 0.21270922949258164 1101 4 0.14805686422737097 1101 5 0.1553876859228538 1101 6 0.1715875704973442 1101 7 0.17921720729274426 1101 8 0.23599928719344376 1101 9 0.3207556654913759 1101 10-11 0.2662098796391774 1101 12-13 0.2795929238645982 1101 14-15 0.4269244001952899 1101 16-17 0.41353384876428834 1101 18-19 0.3678796032008478 1101 20-21 0.3299052029731442 1101 22-23 0.363775444074129 1101 24-25 0.34802271260156203 1101 26-27 0.34980007824130865 1101 28-29 0.3307819419606872 1101 30-31 0.3214125880263694 1101 32-33 0.3976803708835135 1101 34-35 0.422855989274872 1101 36-37 0.4621348569159167 1101 38-39 0.37713594723820165 1101 40-41 0.502688460329928 1101 42-43 0.49272197469013435 1101 44-45 0.49603810463365505 1101 46-47 0.4978654596153973 1101 48-49 0.47202663418347157 1101 50-51 0.37398947291713114 1101 52-53 0.36113767545392506 1101 54-55 0.2974778236867408 1101 56-57 0.3289814342853461 1101 58-59 0.3420168295661412 1101 60-61 0.35948282822930366 1101 62-63 0.34016666031533305 1101 64-65 0.3322489804190525 1101 66-67 0.324236526482359 1101 68-69 0.27697595469761893 1101 70-71 0.27535509253134194 1101 72-73 0.2677969580923971 1101 74-75 0.27939315516469776 1101 76-77 0.33453915780702204 1101 78-79 0.23958948806680525 1101 80-81 0.294768473272093 1101 82-83 0.2707322496828688 1101 84-85 0.23313165937663527 1101 86-87 0.2945293209433224 1101 88-89 0.27431442894939906 1101 90-91 0.30232845455807933 1101 92-93 0.32439593999650995 1101 94-95 0.36156742263058206 1101 96-97 0.4055131919454915 1101 98-99 0.4023134469188179 1101 100 0.427370411169516 1102 1 0.1748866737210335 1102 2 0.197652176053964 1102 3 0.22409251182448742 1102 4 0.1789311506162079 1102 5 0.16670832304813388 1102 6 0.14229024727404038 1102 7 0.1957265297153512 1102 8 0.19311397998767887 1102 9 0.24502751271520395 1102 10-11 0.26786456111496193 1102 12-13 0.26924232262803116 1102 14-15 0.40145005952635415 1102 16-17 0.3769273651665088 1102 18-19 0.3634222469490851 1102 20-21 0.3332532769724068 1102 22-23 0.32567707021234327 1102 24-25 0.3115590976518803 1102 26-27 0.3621291914780187 1102 28-29 0.3013393634440362 1102 30-31 0.3138507902480754 1102 32-33 0.37025337353816923 1102 34-35 0.41042463113932115 1102 36-37 0.4219965360965645 1102 38-39 0.3849710582160242 1102 40-41 0.39103273990175325 1102 42-43 0.422999789339606 1102 44-45 0.4697574841018195 1102 46-47 0.45338208182106143 1102 48-49 0.4415506128761777 1102 50-51 0.40653878650948627 1102 52-53 0.3812556224474477 1102 54-55 0.31300187631467224 1102 56-57 0.3550993059218115 1102 58-59 0.33962850856905646 1102 60-61 0.35675127040433097 1102 62-63 0.35779161245125124 1102 64-65 0.37613465591295636 1102 66-67 0.32717367433158273 1102 68-69 0.3474989018517789 1102 70-71 0.33012062054478974 1102 72-73 0.2945446078646299 1102 74-75 0.34353478837685714 1102 76-77 0.3049679881974896 1102 78-79 0.33490459311762777 1102 80-81 0.3380575551553875 1102 82-83 0.3025206296724079 1102 84-85 0.30032554106084675 1102 86-87 0.31562494616838066 1102 88-89 0.2890271068192156 1102 90-91 0.2767001414162742 1102 92-93 0.30575739992739415 1102 94-95 0.3766108285988423 1102 96-97 0.3760681590773203 1102 98-99 0.44077281357222375 1102 100 0.40593994469140426 1103 1 0.12815994774942396 1103 2 0.18028796611658038 1103 3 0.18514341280246782 1103 4 0.17582485387106317 1103 5 0.1615006578416569 1103 6 0.1305343903982532 1103 7 0.1183618181814694 1103 8 0.1652483300558032 1103 9 0.21402266104600187 1103 10-11 0.23727725906304897 1103 12-13 0.20781268027877786 1103 14-15 0.2959402511866429 1103 16-17 0.31989440274610814 1103 18-19 0.3084967264985181 1103 20-21 0.2917479675472876 1103 22-23 0.25460528291335294 1103 24-25 0.3282367555681702 1103 26-27 0.31724833734431 1103 28-29 0.24951878097525793 1103 30-31 0.2401052349594721 1103 32-33 0.26750453960269027 1103 34-35 0.25579632953262177 1103 36-37 0.2902873309691998 1103 38-39 0.24325429901921325 1103 40-41 0.25126186080019863 1103 42-43 0.32281165136337364 1103 44-45 0.32509795451159107 1103 46-47 0.23623632501639236 1103 48-49 0.17292257293783564 1103 50-51 0.18073708502343777 1103 52-53 0.180223947807292 1103 54-55 0.13050078717579794 1103 56-57 0.18106057861226077 1103 58-59 0.16597889507011843 1103 60-61 0.19172559697992142 1103 62-63 0.19077941062532489 1103 64-65 0.17536427310380986 1103 66-67 0.1852097511685642 1103 68-69 0.16707544374204986 1103 70-71 0.19143056402742786 1103 72-73 0.1648515292611208 1103 74-75 0.1329319682651402 1103 76-77 0.1285474796847197 1103 78-79 0.11254752045311989 1103 80-81 0.04281592479389218 1103 82-83 0.013374559075252535 1103 84-85 0.0614454312020456 1103 86-87 0.02729884833178886 1103 88-89 0.03156616995613959 1103 90-91 0.01912645993568063 1103 92-93 0.07826472562637576 1103 94-95 0.09932286591290307 1103 96-97 0.07991753239928912 1103 98-99 0.12362546226693638 1103 100 0.11775982387737116 1104 1 0.09849418118629671 1104 2 0.17320948535680714 1104 3 0.12628779612514762 1104 4 0.0814832809813737 1104 5 0.07957065849082312 1104 6 0.0806863390118906 1104 7 0.0777902674035289 1104 8 0.12926275872385418 1104 9 0.21772557833324413 1104 10-11 0.20668316715438095 1104 12-13 0.19675893863772842 1104 14-15 0.32374984485446134 1104 16-17 0.24748880038036702 1104 18-19 0.27845542837962967 1104 20-21 0.205549229221198 1104 22-23 0.1940429000808237 1104 24-25 0.23208725123032536 1104 26-27 0.1606103970339916 1104 28-29 0.19160209670977935 1104 30-31 0.16653280752183264 1104 32-33 0.1765189405585943 1104 34-35 0.18756054648935816 1104 36-37 0.216920371643063 1104 38-39 0.2085841790994749 1104 40-41 0.23724478195821774 1104 42-43 0.22659358333124402 1104 44-45 0.30247757338727865 1104 46-47 0.31556955208795046 1104 48-49 0.3053208822287985 1104 50-51 0.26969843854371334 1104 52-53 0.24617960976054576 1104 54-55 0.29686991448435407 1104 56-57 0.3154149201163463 1104 58-59 0.3563359187285897 1104 60-61 0.33445359618843895 1104 62-63 0.3229595742901239 1104 64-65 0.27308494515478543 1104 66-67 0.24434457577260815 1104 68-69 0.22673331138535957 1104 70-71 0.24376820217332806 1104 72-73 0.2658546901668757 1104 74-75 0.23608530573555697 1104 76-77 0.24277394137827457 1104 78-79 0.2728368238861236 1104 80-81 0.2199828544712119 1104 82-83 0.2334923191711873 1104 84-85 0.21477933406794847 1104 86-87 0.20105775627665778 1104 88-89 0.17262349251727827 1104 90-91 0.2224219384650361 1104 92-93 0.28244527305674794 1104 94-95 0.21191094995374016 1104 96-97 0.26369701231921994 1104 98-99 0.2687690217422869 1104 100 0.3051584634844886 1105 1 0.08941770760787193 1105 2 0.1140652935306079 1105 3 0.08774748444156444 1105 4 0.08523659389251037 1105 5 0.06726460318313343 1105 6 0.09389585721112326 1105 7 0.10200659072800988 1105 8 0.11181606935654287 1105 9 0.1470947291958069 1105 10-11 0.1264587639603647 1105 12-13 0.05022060332420608 1105 14-15 0.17243178178726026 1105 16-17 0.1607519498544434 1105 18-19 0.1693298835618009 1105 20-21 0.11494755304355664 1105 22-23 0.111505100454103 1105 24-25 0.11476617722523486 1105 26-27 0.10267730618062387 1105 28-29 0.15664534068081792 1105 30-31 0.18886762328921236 1105 32-33 0.18484911128669523 1105 34-35 0.22287198063585123 1105 36-37 0.25356673504139593 1105 38-39 0.23101432683648682 1105 40-41 0.20962652196134002 1105 42-43 0.26704821792655764 1105 44-45 0.32752348202144077 1105 46-47 0.30389007131480383 1105 48-49 0.3288168169744097 1105 50-51 0.29529477506694235 1105 52-53 0.26149838690988503 1105 54-55 0.3226072615752926 1105 56-57 0.29548819115402836 1105 58-59 0.20689717239844896 1105 60-61 0.19723408609711157 1105 62-63 0.20707937133505538 1105 64-65 0.21758114511481352 1105 66-67 0.22101182582334644 1105 68-69 0.20999095501568377 1105 70-71 0.18503968715434382 1105 72-73 0.19667278681621525 1105 74-75 0.1741318879529956 1105 76-77 0.22217403600918573 1105 78-79 0.2078779609142174 1105 80-81 0.18529765058755032 1105 82-83 0.22422361678201597 1105 84-85 0.199139758268295 1105 86-87 0.17290388772348209 1105 88-89 0.1964936036788636 1105 90-91 0.17958425500810904 1105 92-93 0.20445980971113897 1105 94-95 0.2339637132882757 1105 96-97 0.20731813615607564 1105 98-99 0.2629073731552083 1105 100 0.3162094605798984 1106 1 0.07845534894207162 1106 2 0.14886168171518932 1106 3 0.12437463244438618 1106 4 0.1270898008940904 1106 5 0.08440218770296326 1106 6 0.09817731104851646 1106 7 0.10183905491673073 1106 8 0.13761053244547128 1106 9 0.1485650239621208 1106 10-11 0.19096967647492136 1106 12-13 0.19866759592410688 1106 14-15 0.24283874448920528 1106 16-17 0.22943673625860583 1106 18-19 0.1839059553120137 1106 20-21 0.15276819222687976 1106 22-23 0.1474843966893502 1106 24-25 0.09854518906624321 1106 26-27 0.1698449188108242 1106 28-29 0.0903187675548267 1106 30-31 0.08658891121231704 1106 32-33 0.08604695736431722 1106 34-35 0.15481368206616963 1106 36-37 0.1087785554729308 1106 38-39 0.05651721861589465 1106 40-41 0.07640666127060314 1106 42-43 0.05069472472430192 1106 44-45 0.0702197539193179 1106 46-47 0.11344839837985887 1106 48-49 0.09672728929159291 1106 50-51 0.09432849881842031 1106 52-53 0.05462949163476338 1106 54-55 0.029898204769978065 1106 56-57 -0.03748088585290077 1106 58-59 -0.03568058669193164 1106 60-61 -0.016296798182381167 1106 62-63 -0.04597776947670695 1106 64-65 -0.047709713658122155 1106 66-67 -0.018260545073260914 1106 68-69 0.042431529373089916 1106 70-71 0.01248926659961569 1106 72-73 0.12239049788473011 1106 74-75 0.10728823161787204 1106 76-77 0.11773259444335338 1106 78-79 0.14606822503081673 1106 80-81 0.11922895001768552 1106 82-83 0.049877363778673356 1106 84-85 0.08516965281227229 1106 86-87 0.10265609772732986 1106 88-89 0.0534793604283017 1106 90-91 0.1504463744039377 1106 92-93 0.17666011721796693 1106 94-95 0.16666242782118346 1106 96-97 0.11205543094143522 1106 98-99 0.15832506648177258 1106 100 0.20185366390469284 1107 1 0.06515946145639617 1107 2 0.09244933141571465 1107 3 0.02543191514948262 1107 4 0.09545770232954709 1107 5 0.10749496561633975 1107 6 0.054054390634000526 1107 7 0.008111435289400504 1107 8 0.04273683684766638 1107 9 0.024081185039534603 1107 10-11 0.05924928686935971 1107 12-13 0.0307787662977006 1107 14-15 0.12314838589097832 1107 16-17 0.10369608089600035 1107 18-19 0.07147148303430129 1107 20-21 0.027522730075013158 1107 22-23 0.06451442267444207 1107 24-25 0.11245504582181098 1107 26-27 0.09986296875402445 1107 28-29 0.10207157072003525 1107 30-31 0.09490549536351978 1107 32-33 0.1081021397671762 1107 34-35 0.10702025497759138 1107 36-37 0.08585176270776884 1107 38-39 0.009743306961176756 1107 40-41 -0.02042445373776758 1107 42-43 -0.024509605253165034 1107 44-45 -0.003873153567937493 1107 46-47 -0.04347988699606731 1107 48-49 0.04035687472547522 1107 50-51 -0.0035010382010369767 1107 52-53 0.04591891247968505 1107 54-55 0.07579447937018102 1107 56-57 0.1587042681943771 1107 58-59 0.12803425634789534 1107 60-61 0.11548963392542078 1107 62-63 0.12672161610201726 1107 64-65 0.14290677677801966 1107 66-67 0.169198963088391 1107 68-69 0.09691031591869859 1107 70-71 0.10642154265024573 1107 72-73 0.16044705035562146 1107 74-75 0.23283352170768268 1107 76-77 0.20575117875326754 1107 78-79 0.19640642290922017 1107 80-81 0.17066626588968603 1107 82-83 0.16613351226029494 1107 84-85 0.1814169320118495 1107 86-87 0.1564732036896359 1107 88-89 0.14840578958302686 1107 90-91 0.1984065399772632 1107 92-93 0.15390232294713968 1107 94-95 0.13285670226268564 1107 96-97 0.10272460471206202 1107 98-99 0.14787729329432153 1107 100 0.12847717945506432 1108 1 0.05915041462383641 1108 2 0.09535019267065792 1108 3 0.08355971322050237 1108 4 0.11074415737297727 1108 5 0.051109337725904425 1108 6 0.0674037518813222 1108 7 0.0988897785910936 1108 8 0.1142459021521205 1108 9 0.15307631181997294 1108 10-11 0.0989878050416877 1108 12-13 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-0.2092299851405528 2215 5 -0.20077827371922297 2215 6 -0.3016392328013353 2215 7 -0.31614485727693165 2215 8 -0.3292052046575833 2215 9 -0.37942786046228605 2215 10-11 -0.3599832115769459 2215 12-13 -0.36628689653169033 2215 14-15 -0.4663196627363675 2215 16-17 -0.5170654388105191 2215 18-19 -0.4533112284972418 2215 20-21 -0.5166477750022054 2215 22-23 -0.5036331048560854 2215 24-25 -0.5736766789394352 2215 26-27 -0.4874679549571397 2215 28-29 -0.49735617918856434 2215 30-31 -0.5124567413986583 2215 32-33 -0.540023419150188 2215 34-35 -0.500605724732587 2215 36-37 -0.4674551091785375 2215 38-39 -0.5685466093234481 2215 40-41 -0.5535451317398525 2215 42-43 -0.5270203904384161 2215 44-45 -0.49326387511565173 2215 46-47 -0.5463781869180551 2215 48-49 -0.5378066122138421 2215 50-51 -0.5168402720915708 2215 52-53 -0.5580453060224286 2215 54-55 -0.5532104681403425 2215 56-57 -0.5483519821949798 2215 58-59 -0.5858652998948699 2215 60-61 -0.5200852623888323 2215 62-63 -0.5329955206121468 2215 64-65 -0.5184409272142645 2215 66-67 -0.558007057027595 2215 68-69 -0.6513774751023078 2215 70-71 -0.5551036509468901 2215 72-73 -0.5543787815944157 2215 74-75 -0.5533219100213671 2215 76-77 -0.5672370958726916 2215 78-79 -0.537241006252632 2215 80-81 -0.5289763201098232 2215 82-83 -0.485119971190592 2215 84-85 -0.4153606868596782 2215 86-87 -0.5022173540978763 2215 88-89 -0.4865933744591082 2215 90-91 -0.47204038365480017 2215 92-93 -0.448610244364275 2215 94-95 -0.5308172430605893 2215 96-97 -0.5988351543163404 2215 98-99 -0.6024119113904476 2215 100 -0.6288434313733688 2216 1 -0.2178826742476616 2216 2 -0.2702985074530915 2216 3 -0.243782631870765 2216 4 -0.1359420067598407 2216 5 -0.24874420455147828 2216 6 -0.29784617558519244 2216 7 -0.29423177749345086 2216 8 -0.3545113754203797 2216 9 -0.7236138677785746 2216 10-11 -0.572686699142281 2216 12-13 -0.5124767864291186 2216 14-15 -0.6556587414657002 2216 16-17 -0.49443658156498316 2216 18-19 -0.5465790724465336 2216 20-21 -0.6841456046191681 2216 22-23 -0.6642067589892449 2216 24-25 -0.536609534897714 2216 26-27 -0.647676063632808 2216 28-29 -0.6208780314743976 2216 30-31 -0.6135173221772305 2216 32-33 -0.6113516894329081 2216 34-35 -0.6717620497715231 2216 36-37 -0.6579441109577786 2216 38-39 -0.6666926847648256 2216 40-41 -0.8570222961050078 2216 42-43 -0.8897136023549237 2216 44-45 -0.8021644505910785 2216 46-47 -0.794148119348705 2216 48-49 -0.49197516633256555 2216 50-51 -0.5528443612893597 2216 52-53 -0.6522129820318838 2216 54-55 -0.5846672723271027 2216 56-57 -0.9021279014499513 2216 58-59 -0.9770756273887002 2216 60-61 -0.7642120588367831 2216 62-63 -0.8891550788360405 2216 64-65 -0.8445697005692097 2216 66-67 -0.770536244848266 2216 68-69 -0.6817394593772335 2216 70-71 -0.7545980092385989 2216 72-73 -0.6865518782902953 2216 74-75 -0.6655606158957212 2216 76-77 -0.6310190126667905 2216 78-79 -0.5944120410357598 2216 80-81 -0.6898601693858097 2216 82-83 -0.7178449477151361 2216 84-85 -0.7188695415649278 2216 86-87 -0.7243323323310484 2216 88-89 -0.6227625810780815 2216 90-91 -0.697122073223472 2216 92-93 -0.5886757820339739 2216 94-95 -0.7154388585604323 2216 96-97 -0.8994752600978124 2216 98-99 -0.9170283308931566 2216 100 -0.9695467548134289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 251.0 3 521.0 4 466.0 5 528.0 6 1214.0 7 2824.0 8 5166.0 9 8488.0 10 11201.0 11 13764.0 12 19008.0 13 26985.0 14 27258.0 15 33677.0 16 45090.0 17 68794.0 18 73390.0 19 37988.0 20 24128.0 21 21344.0 22 21124.0 23 22021.0 24 24490.0 25 28820.0 26 35028.0 27 43796.0 28 54444.0 29 67832.0 30 84619.0 31 106322.0 32 134106.0 33 169815.0 34 231109.0 35 361153.0 36 535367.0 37 999939.0 38 1413469.0 39 686486.0 40 7276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.08430677894155 21.133280162860277 15.563397710732064 22.219015347466105 2 13.967773114386597 22.373823725281465 40.211689535960666 23.446713624371274 3 15.277804029918753 30.23378637698005 31.62262760421493 22.865781988886262 4 10.278440305310315 18.967598057543547 38.81594556655837 31.93801607058777 5 10.626516778710702 39.29578157505678 34.80494207428527 15.272759571947248 6 25.929527475175256 40.35794682657464 19.10256012651898 14.609965571731125 7 23.617744734599906 34.813547645835676 23.947603555024763 17.621104064539654 8 25.922370593953243 33.456456158321956 21.260286411046113 19.360886836678688 9 26.03062301018057 15.222025724033228 22.880053790385226 35.867297475400974 10-11 22.935215250984296 27.645184927223433 28.970749431400804 20.448850390391467 12-13 24.98953535508499 26.12392855524039 29.536623137536356 19.349912952138265 14-15 21.921362024230266 26.516208225605446 29.684412734770937 21.87801701539335 16-17 18.649373561856834 29.45690649130962 30.125102283760796 21.76861766307275 18-19 18.771416737669657 26.257872339956993 35.51118758167332 19.459523340700027 20-21 20.849398849503817 25.220922830285943 35.17266709987207 18.757011220338168 22-23 19.94178702919879 25.59531029759597 35.499167691415835 18.963734981789408 24-25 19.173585173655276 25.779659048096875 35.600464502329025 19.446291275918824 26-27 19.001840786552258 25.8745479466082 35.56112976691873 19.562481499920818 28-29 19.04509796383248 25.669897948931432 35.961796064306824 19.32320802292926 30-31 19.606195988278078 26.061786459853554 35.29185014093595 19.040167410932415 32-33 18.91316166272961 25.85642081059147 35.71303063654594 19.517386890132986 34-35 19.20341105574337 25.527322982987293 35.56616889530285 19.70309706596648 36-37 19.629493215765383 25.96917768499188 34.78991224979372 19.61141684944901 38-39 19.490405300195945 25.97478635752833 34.940902227974306 19.59390611430142 40-41 19.424532384160866 25.854156122378946 34.78294579902732 19.93836569443287 42-43 19.346836053045234 26.04391269651858 34.728116437674274 19.881134812761914 44-45 19.803062814845426 26.07941825933271 34.07713209455672 20.04038683126515 46-47 19.84638694696443 26.492773857226737 33.33304889190335 20.327790303905484 48-49 19.895363908577064 26.5422487097806 32.72714477011312 20.83524261152922 50-51 20.15491243451456 26.62447704551533 32.157254059420566 21.06335646054954 52-53 20.16411980288667 26.979707853427932 31.821189737819015 21.034982605866386 54-55 20.271762792500787 27.365608491096232 31.64660193283923 20.716026783563755 56-57 20.228943926172697 26.900379110954304 31.66561523748 21.205061725393 58-59 20.14467907125187 26.3214217907974 32.20463298603175 21.32926615191899 60-61 20.358387887622033 26.858483176218023 31.77787049210848 21.005258444051464 62-63 20.101019443586512 26.75868286956367 32.063162523175635 21.077135163674182 64-65 20.164138379942095 26.644808586733188 32.09239961473551 21.098653418589205 66-67 20.124997763473182 26.643828860509082 31.986086279928745 21.24508709608899 68-69 20.594119256020015 26.827067053789598 31.762600037674844 20.81621365251554 70-71 20.624131823617482 26.653651495155827 31.604460147635976 21.11775653359071 72-73 20.383476706461984 26.652886305968416 31.766881660601975 21.196755326967622 74-75 20.401835724882766 26.71905145834096 31.44829011257702 21.43082270419925 76-77 20.428840322823056 26.68271581988222 31.310538727811142 21.57790512948358 78-79 20.575311289059318 26.716897804405576 31.524637401372217 21.18315350516289 80-81 20.15014402764685 26.912387478687634 31.478817191415924 21.45865130224959 82-83 20.073784411591017 26.960589467842755 31.555768918094106 21.409857202472118 84-85 20.118234066161637 26.64793695969308 31.34352447403329 21.890304500111988 86-87 20.322606514119883 26.827275645078146 31.13112122086851 21.718996619933456 88-89 19.952494824565573 26.779957649614143 31.378795188593912 21.88875233722637 90-91 19.884596207843906 26.73616304182867 31.241896896501036 22.13734385382639 92-93 19.84560002649887 26.920165816142788 31.235967778178747 21.998266379179594 94-95 19.811430860581936 26.571316211014956 31.4020825790317 22.215170349371416 96-97 19.873693382188726 26.889750001789224 31.156433722699827 22.08012289332222 98-99 19.670653435548765 26.800244069165164 31.116157653999842 22.412944841286226 100 19.63452052381232 26.819781695930473 31.237080862058843 22.308616918198368 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7956.0 1 4859.0 2 15504.0 3 24029.0 4 12258.5 5 5739.0 6 9060.0 7 24065.5 8 39005.0 9 38330.5 10 24490.5 11 11970.5 12 9349.0 13 9511.5 14 10113.5 15 10772.0 16 11361.5 17 12356.0 18 13420.0 19 14386.5 20 15902.0 21 18100.5 22 21768.0 23 27547.0 24 33978.5 25 41219.5 26 51133.0 27 63300.5 28 77475.0 29 90465.5 30 100454.5 31 110430.5 32 121169.5 33 130754.0 34 140301.5 35 150499.0 36 155954.5 37 160713.0 38 167664.0 39 170552.0 40 173811.5 41 176326.5 42 175235.5 43 181344.0 44 183839.0 45 178400.0 46 176587.0 47 177752.5 48 178253.5 49 177035.0 50 181699.0 51 172632.5 52 149209.0 53 131186.5 54 120766.5 55 117324.5 56 104960.0 57 88943.5 58 81157.0 59 76482.5 60 66457.0 61 53028.0 62 42783.0 63 33270.0 64 25283.0 65 19149.5 66 14307.0 67 10563.0 68 7601.0 69 5565.5 70 3919.0 71 2839.5 72 2138.0 73 1534.0 74 1213.0 75 958.5 76 582.0 77 434.5 78 371.5 79 218.5 80 93.0 81 24.5 82 16.0 83 12.5 84 8.5 85 6.5 86 3.5 87 3.0 88 3.0 89 3.0 90 3.0 91 2.5 92 3.0 93 4.0 94 4.5 95 4.5 96 2.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.048978758927062396 2 0.0 3 1.8350977492342596E-4 4 0.07019248890821043 5 0.004642797305562677 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011074814916628756 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.138273697855927E-4 26-27 0.0 28-29 3.578440611006806E-4 30-31 0.0035692651222606346 32-33 1.5598330868491208E-4 34-35 0.001449727221895065 36-37 0.0036151425659914915 38-39 0.0012570419582254677 40-41 0.0011561115820175837 42-43 0.0018809751929651161 44-45 0.0 46-47 4.495989485623936E-4 48-49 6.239332347396483E-4 50-51 0.0017158163955340326 52-53 9.175488746171297E-4 54-55 0.0011652870707637548 56-57 0.006762335205928247 58-59 0.008854346640055303 60-61 0.00120198902574844 62-63 0.0027893485788360746 64-65 0.0010551812058096992 66-67 6.422842122319908E-5 68-69 7.615655659322177E-4 70-71 3.578440611006806E-4 72-73 0.0 74-75 1.7433428617725467E-4 76-77 0.0 78-79 4.5877443730856486E-4 80-81 0.0 82-83 8.62495942140102E-4 84-85 4.679499260547362E-4 86-87 0.0 88-89 0.0 90-91 0.0 92-93 2.2938721865428243E-4 94-95 0.0 96-97 4.037215048315371E-4 98-99 4.771254148009075E-4 100 3.853705273391945E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5449301.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.918597650226054 #Duplication Level Percentage of deduplicated Percentage of total 1 69.44249805090121 31.887021278259393 2 17.102383627203178 15.706349452747126 3 6.144712484175912 8.464697407115843 4 2.778452409701599 5.1033055316555505 5 1.3826047103202443 3.174363470125132 6 0.8117107367516 2.236357123755917 7 0.5127621027157705 1.6481721679422543 8 0.3353947921021546 1.2320686810016044 9 0.22259195350001026 0.9198999317650318 >10 1.0641261844536205 8.783549734617662 >50 0.10200432241322727 3.284931543358442 >100 0.0897658119053004 8.155561085283058 >500 0.007341919674578591 2.2508161910061393 >1k 0.0032898167349316794 2.4412273840845464 >5k 8.023943255879456E-5 0.2725411497653 >10k+ 2.808380139557809E-4 4.439137867516949 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63783 1.1704803973940878 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49120 0.9014000144238683 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45807 0.8406032259917373 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43125 0.7913859043572745 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16453 0.30192863268151277 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11704 0.21477984057037772 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10972 0.20134692504598295 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9781 0.17949091085260294 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 7.340390996937038E-5 0.0 6 0.0 0.0 0.0 7.340390996937038E-5 0.0 7 0.0 0.0 0.0 7.340390996937038E-5 0.0 8 0.0 0.0 0.0 7.340390996937038E-5 0.0 9 0.0 0.0 0.0 9.175488746171298E-5 0.0 10-11 0.0 0.0 0.0 1.2845684244639817E-4 0.0 12-13 0.0 0.0 0.0 1.6515879743108337E-4 0.0 14-15 0.0 0.0 0.0 2.5691368489279634E-4 0.0 16-17 0.0 0.0 0.0 3.3949308360833804E-4 0.0 18-19 0.0 0.0 0.0 4.037215048315371E-4 0.0 20-21 0.0 0.0 0.0 5.413538360241066E-4 0.0 22-23 0.0 0.0 0.0 9.634263183479863E-4 0.0 24-25 0.0 0.0 0.0 0.0017800448167572319 0.0 26-27 0.0 0.0 0.0 0.0027893485788360746 0.0 28-29 0.0 0.0 0.0 0.004596919861831821 0.0 30-31 0.0 0.0 0.0 0.010524285591858478 0.0 32-33 0.0 0.0 0.0 0.020213601707815367 0.0 34-35 0.0 0.0 0.0 0.03231607136401531 0.0 36-37 0.0 0.0 0.0 0.051006541939966246 0.0 38-39 0.0 0.0 0.0 0.08628629616899489 0.0 40-41 0.0 0.0 0.0 0.13672395780669852 0.0 42-43 0.0 0.0 0.0 0.18694140771449402 0.0 44-45 0.0 0.0 0.0 0.23996655717861795 0.0 46-47 0.0 0.0 0.0 0.3038830117844472 0.0 48-49 0.0 0.0 0.0 0.3800028664226843 0.0 50-51 0.0 0.0 0.0 0.46754620454990464 0.0 52-53 0.0 0.0 0.0 0.5579155932109457 0.0 54-55 0.0 0.0 0.0 0.6536526427884971 0.0 56-57 0.0 0.0 0.0 0.7617307247296488 0.0 58-59 0.0 0.0 0.0 0.8882698900280972 0.0 60-61 0.0 0.0 0.0 1.026617909342868 0.0 62-63 0.0 0.0 0.0 1.1711593835613046 0.0 64-65 0.0 0.0 0.0 1.3203253041078113 0.0 66-67 0.0 0.0 0.0 1.4915949770438446 0.0 68-69 0.0 0.0 0.0 1.6859226531990066 0.0 70-71 0.0 0.0 0.0 1.9035377197919514 0.0 72-73 0.0 0.0 0.0 2.149688556385489 0.0 74-75 0.0 0.0 0.0 2.390480907551262 0.0 76-77 0.0 0.0 0.0 2.635035209102966 0.0 78-79 1.8350977492342595E-5 0.0 0.0 2.916796117520394 0.0 80-81 1.8350977492342595E-5 0.0 0.0 3.2305886571507063 0.0 82-83 1.8350977492342595E-5 0.0 0.0 3.571421362115985 0.0 84-85 1.8350977492342595E-5 0.0 0.0 3.911235587830439 0.0 86-87 1.8350977492342595E-5 0.0 0.0 4.26198699613033 0.0 88 1.8350977492342595E-5 0.0 0.0 4.5423624057470855 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22920 0.0 52.45502 1 TACCTGG 5025 0.0 42.771996 2 GTATCAA 42395 0.0 41.988705 1 TATAACG 725 0.0 38.273083 2 GTACCTG 6310 0.0 34.751003 1 ACCTGGG 6275 0.0 33.35229 3 ATAACGC 920 0.0 33.228058 3 TAACGCA 910 0.0 32.042744 4 TATCAAC 58775 0.0 30.118713 2 ATCAACG 59045 0.0 30.028778 3 TCAACGC 59860 0.0 29.643503 4 CAACGCA 62250 0.0 28.60426 5 AACGCAG 64090 0.0 27.906706 6 ACGCAGA 69765 0.0 25.535585 7 CGCAGAG 69615 0.0 25.50283 8 CATGGGT 14065 0.0 24.476582 4 TACACGG 520 0.0 24.419672 2 GCAGAGT 72370 0.0 24.259186 9 GTATAAC 1625 0.0 23.165804 1 AGTACTT 51080 0.0 22.89974 12-13 >>END_MODULE