FastQCFastQC Report
Wed 25 May 2016
SRR2431457_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431457_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4573485
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA462151.0104985585390571No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59500.13009772635091185No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71300.045.6236341
GTATCAA115000.033.3144841
TACCTGG43200.032.3156432
GTACCTG48950.029.2900771
ATCAACG130650.028.0621263
TCAACGC130650.028.0621264
TATCAAC139250.026.9031542
TCTGTCG12550.025.8406098
CAACGCA146150.025.0859835
GTACATG219300.024.6508871
CATGGGT104650.024.5238554
AACGCAG153350.023.9990656
TACATGG218500.023.7927042
ACCTGGG60150.022.6619723
CGCAGAG159100.022.392188
GTCTCGC11650.022.1926981
ACATGGG224600.022.1207893
ACGCAGA164250.022.0625557
CTGTCGC14650.021.1740389
GTGGTAT39450.020.733641
Produced by FastQC (version 0.11.5)