##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431457_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4573485
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.893241805756443	31.0	30.0	34.0	25.0	34.0
2	29.833565869353457	31.0	30.0	34.0	25.0	34.0
3	29.96497681745977	31.0	30.0	34.0	25.0	34.0
4	33.21839319468633	37.0	35.0	37.0	26.0	37.0
5	33.3371050741393	37.0	35.0	37.0	27.0	37.0
6	33.35446601442882	37.0	35.0	37.0	27.0	37.0
7	33.16576374471546	37.0	35.0	37.0	26.0	37.0
8	33.10936124202878	37.0	35.0	37.0	25.0	37.0
9	34.3701363402307	39.0	35.0	39.0	23.0	39.0
10-11	34.34538311593894	39.0	35.0	39.0	23.0	39.0
12-13	34.32269450976662	39.0	35.0	39.0	23.0	39.0
14-15	35.48816602656399	40.0	36.0	41.0	23.5	41.0
16-17	35.50608135808907	40.0	36.0	41.0	24.5	41.0
18-19	35.49523886051884	40.0	36.0	41.0	24.0	41.0
20-21	35.604565664914176	40.0	36.0	41.0	25.0	41.0
22-23	35.5699146274668	40.0	36.0	41.0	25.0	41.0
24-25	35.6179992937552	40.0	36.0	41.0	25.5	41.0
26-27	35.62407245240773	40.0	35.0	41.0	26.0	41.0
28-29	35.521011110783135	40.0	35.0	41.0	25.5	41.0
30-31	35.50018825906284	40.0	35.0	41.0	26.0	41.0
32-33	35.44028776742462	40.0	35.0	41.0	25.5	41.0
34-35	35.335074347024204	40.0	35.0	41.0	25.0	41.0
36-37	35.207574420819135	40.0	35.0	41.0	25.0	41.0
38-39	35.099618671538224	40.0	35.0	41.0	25.0	41.0
40-41	35.005388123061515	40.0	35.0	41.0	24.5	41.0
42-43	34.784581451562644	39.0	34.0	41.0	24.0	41.0
44-45	34.64928047211262	39.0	34.0	41.0	23.5	41.0
46-47	34.514444018073746	39.0	34.0	41.0	23.0	41.0
48-49	34.38375494835995	39.0	33.5	41.0	22.5	41.0
50-51	34.22090178496267	39.0	33.0	41.0	22.0	41.0
52-53	34.12011365512295	39.0	33.0	40.5	22.0	41.0
54-55	34.05103460490195	39.0	33.0	41.0	21.0	41.0
56-57	33.89382221653728	38.0	33.0	40.0	19.5	41.0
58-59	33.681079198904115	38.0	33.0	40.0	18.5	41.0
60-61	33.46138994661621	38.0	33.0	40.0	17.5	41.0
62-63	33.18046773959027	37.0	32.5	40.0	14.0	41.0
64-65	33.03881580457791	37.0	32.0	40.0	12.5	41.0
66-67	32.78716416474526	36.5	32.0	40.0	8.0	41.0
68-69	32.52355053094085	36.0	32.0	39.5	7.5	41.0
70-71	32.23046746627572	36.0	31.5	39.0	4.5	41.0
72-73	31.925599734119604	35.0	31.0	39.0	2.0	41.0
74-75	31.59634184872149	35.0	31.0	38.5	2.0	40.0
76-77	31.269528925972207	35.0	31.0	37.5	2.0	40.0
78-79	30.924109513860873	35.0	31.0	37.0	2.0	39.0
80-81	30.613803587417472	35.0	31.0	36.5	2.0	39.0
82-83	30.297051263970474	35.0	30.0	36.0	2.0	38.5
84-85	29.989062826269247	35.0	30.0	36.0	2.0	37.0
86-87	29.71173426828775	35.0	30.0	35.0	2.0	37.0
88-89	29.421913267453593	34.5	30.0	35.0	2.0	36.5
90-91	29.17507874192219	34.0	30.0	35.0	2.0	36.0
92-93	28.959157076059064	34.0	29.0	35.0	2.0	36.0
94-95	28.715634904236047	34.0	29.0	35.0	2.0	36.0
96-97	28.60214683113643	34.0	29.0	35.0	2.0	35.5
98-99	28.426605422342043	34.0	29.0	35.0	2.0	35.0
100	28.274586885055925	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6278842546614456
1101	2	0.7399062056827468
1101	3	0.7112209824241624
1101	4	0.7092603248706837
1101	5	0.5543070858641244
1101	6	0.520417205811512
1101	7	0.586482553703334
1101	8	0.7549302172419203
1101	9	0.8795511291026159
1101	10-11	0.9127828725727909
1101	12-13	0.8499803842677736
1101	14-15	0.9777406951157488
1101	16-17	0.9269398721170177
1101	18-19	0.8943650585633947
1101	20-21	0.7610124675009899
1101	22-23	0.8080435446937244
1101	24-25	0.7621105850682355
1101	26-27	0.8432130249140215
1101	28-29	0.9262858408969237
1101	30-31	0.905933180798904
1101	32-33	0.968581702738561
1101	34-35	0.923945548073597
1101	36-37	0.8964170374704423
1101	38-39	0.8888399719644227
1101	40-41	0.8854856002629319
1101	42-43	0.9105090557186628
1101	44-45	0.8829947768878128
1101	46-47	0.7941796466511235
1101	48-49	0.7482841151782864
1101	50-51	0.7768748867673025
1101	52-53	0.7276934270287896
1101	54-55	0.8443307448982438
1101	56-57	0.7538492137871771
1101	58-59	0.8113106965581593
1101	60-61	0.8188189113167184
1101	62-63	0.8661256627446932
1101	64-65	0.8324022289554023
1101	66-67	0.8708011064837748
1101	68-69	0.7981964294799511
1101	70-71	0.7809079184920051
1101	72-73	0.7642486462615459
1101	74-75	0.7297328956292475
1101	76-77	0.7907090925813165
1101	78-79	0.7664576208854861
1101	80-81	0.6991288310071653
1101	82-83	0.7360634571026168
1101	84-85	0.7559920533240216
1101	86-87	0.7196300974770722
1101	88-89	0.6786550933315745
1101	90-91	0.7211024299596289
1101	92-93	0.7158750359385273
1101	94-95	0.7448704902645957
1101	96-97	0.7811975551902108
1101	98-99	0.7507326813186914
1101	100	0.7892132713828808
1102	1	0.3327897521375931
1102	2	0.4136438441068755
1102	3	0.3874004191086371
1102	4	0.37781251146444816
1102	5	0.3246921865669705
1102	6	0.3708402088182936
1102	7	0.3314248574464429
1102	8	0.3173332011531258
1102	9	0.5263905561245537
1102	10-11	0.4603330469297262
1102	12-13	0.46323473567304774
1102	14-15	0.5396635940583749
1102	16-17	0.5146010182393255
1102	18-19	0.49348901539932655
1102	20-21	0.5401664080704549
1102	22-23	0.4645485983342539
1102	24-25	0.5432742152772292
1102	26-27	0.6306077934939296
1102	28-29	0.6466203092418397
1102	30-31	0.6076272058542216
1102	32-33	0.6817543410261777
1102	34-35	0.683563816596326
1102	36-37	0.6659891369193787
1102	38-39	0.6163076576685
1102	40-41	0.7126864929593282
1102	42-43	0.6690422179594293
1102	44-45	0.5572539973483259
1102	46-47	0.5291495471808219
1102	48-49	0.4837117694177948
1102	50-51	0.5430807529787742
1102	52-53	0.4710138334567091
1102	54-55	0.5936125092961859
1102	56-57	0.5964517753482497
1102	58-59	0.5984927122928241
1102	60-61	0.5856382812576868
1102	62-63	0.5484740416585225
1102	64-65	0.5849698043831211
1102	66-67	0.585583969490628
1102	68-69	0.5711888715729927
1102	70-71	0.5345749436458505
1102	72-73	0.536556856607266
1102	74-75	0.5788225271194563
1102	76-77	0.556249398252973
1102	78-79	0.479103632201177
1102	80-81	0.47048476031000774
1102	82-83	0.513470662932427
1102	84-85	0.43202145263641256
1102	86-87	0.44189915022019477
1102	88-89	0.4491915421005501
1102	90-91	0.4922399926899388
1102	92-93	0.4427939365224347
1102	94-95	0.4522378420928632
1102	96-97	0.4801134529493467
1102	98-99	0.5591914406523166
1102	100	0.5613737937137451
1103	1	0.40035220293346896
1103	2	0.46420569368986975
1103	3	0.45363166772477115
1103	4	0.4360125323762034
1103	5	0.3195149902179182
1103	6	0.39787383829894196
1103	7	0.46644712231223195
1103	8	0.46743537659043
1103	9	0.520122796511302
1103	10-11	0.6021277019780626
1103	12-13	0.4695531409366609
1103	14-15	0.6075687438849329
1103	16-17	0.5089058331703171
1103	18-19	0.45736367481453044
1103	20-21	0.5450230088036037
1103	22-23	0.5243785058304908
1103	24-25	0.5711281697511126
1103	26-27	0.6049128621336806
1103	28-29	0.6032456252245666
1103	30-31	0.5632879480369226
1103	32-33	0.7098866063225131
1103	34-35	0.698931359865206
1103	36-37	0.6827999370706834
1103	38-39	0.7171367087054819
1103	40-41	0.6806319286618461
1103	42-43	0.6486721914199549
1103	44-45	0.5699959432483936
1103	46-47	0.5927814342204556
1103	48-49	0.584091544524199
1103	50-51	0.4950261994446876
1103	52-53	0.5807717030411794
1103	54-55	0.6708843174862196
1103	56-57	0.5615721063382395
1103	58-59	0.602276720798983
1103	60-61	0.5683805116190968
1103	62-63	0.46982153886004596
1103	64-65	0.5410733836755668
1103	66-67	0.541215112317893
1103	68-69	0.4468887810066491
1103	70-71	0.46881276793178017
1103	72-73	0.43559249423119795
1103	74-75	0.38984842155816324
1103	76-77	0.3903288659873674
1103	78-79	0.4525859733026074
1103	80-81	0.40443793105447057
1103	82-83	0.48892530134362744
1103	84-85	0.4474415635917097
1103	86-87	0.3931129736573453
1103	88-89	0.41510673110589025
1103	90-91	0.4164815956987731
1103	92-93	0.37914138759508376
1103	94-95	0.44201405174548114
1103	96-97	0.430669171237291
1103	98-99	0.4725840149812939
1103	100	0.47621298848826754
1104	1	0.41653757328983687
1104	2	0.44315813584925223
1104	3	0.4324151718076408
1104	4	0.4035685457841325
1104	5	0.38021298406841453
1104	6	0.46949925099615086
1104	7	0.4588232501412577
1104	8	0.4025844201410109
1104	9	0.5742845801193468
1104	10-11	0.5356080390036198
1104	12-13	0.5257092995762136
1104	14-15	0.5883665096493331
1104	16-17	0.6419546102004148
1104	18-19	0.6052437579210377
1104	20-21	0.5328988467994691
1104	22-23	0.5113438896704068
1104	24-25	0.5929763129286982
1104	26-27	0.6923660792817188
1104	28-29	0.699433413482538
1104	30-31	0.6862847126167892
1104	32-33	0.6923772970075248
1104	34-35	0.6760130786388316
1104	36-37	0.6916668233174619
1104	38-39	0.7044616274261841
1104	40-41	0.6733727107073051
1104	42-43	0.674609699295857
1104	44-45	0.6352532961895747
1104	46-47	0.5826557820958485
1104	48-49	0.5652155694876555
1104	50-51	0.5698830943234654
1104	52-53	0.6062919850614463
1104	54-55	0.5845081298003478
1104	56-57	0.5501821752051157
1104	58-59	0.6287320691658351
1104	60-61	0.6152409412022308
1104	62-63	0.6339783527745553
1104	64-65	0.6507724770018442
1104	66-67	0.7037591386044966
1104	68-69	0.6554719772993067
1104	70-71	0.6095175114294307
1104	72-73	0.6858614562504535
1104	74-75	0.7194082138233995
1104	76-77	0.6074761201332421
1104	78-79	0.6726837368617602
1104	80-81	0.6229412184555478
1104	82-83	0.6241706671634759
1104	84-85	0.5562628877511919
1104	86-87	0.5933583473530959
1104	88-89	0.5984581002525218
1104	90-91	0.5978025575668084
1104	92-93	0.5383292955667542
1104	94-95	0.5623343882669793
1104	96-97	0.5658974069724607
1104	98-99	0.5049407759697502
1104	100	0.45509454753862144
1105	1	0.26074666528131374
1105	2	0.2350202067366709
1105	3	0.3201280650876157
1105	4	0.23412116596549026
1105	5	0.1822796324008351
1105	6	0.32678138219363717
1105	7	0.23238356186147513
1105	8	0.2686763211371783
1105	9	0.33859841844938643
1105	10-11	0.35858636285676937
1105	12-13	0.3028798238322068
1105	14-15	0.48515780003307896
1105	16-17	0.4348739740445069
1105	18-19	0.40075890336519393
1105	20-21	0.3294290260835169
1105	22-23	0.40223665588319335
1105	24-25	0.4386667404806488
1105	26-27	0.5317509888492182
1105	28-29	0.6372479564065969
1105	30-31	0.5560342657754731
1105	32-33	0.5432062768516772
1105	34-35	0.54488564068415
1105	36-37	0.5126501149223941
1105	38-39	0.5376517077499869
1105	40-41	0.4999627878513522
1105	42-43	0.5073272702190508
1105	44-45	0.4781444396522687
1105	46-47	0.4503906751259166
1105	48-49	0.44330987468703853
1105	50-51	0.46141199143130507
1105	52-53	0.4775400788794144
1105	54-55	0.5239841091601178
1105	56-57	0.5041862025156902
1105	58-59	0.5031698610217035
1105	60-61	0.41929461655522005
1105	62-63	0.4828558992743197
1105	64-65	0.5162929476529641
1105	66-67	0.45776333785441636
1105	68-69	0.41923512965390586
1105	70-71	0.49790789036497785
1105	72-73	0.4417180450865139
1105	74-75	0.4846278821832328
1105	76-77	0.4362805861615584
1105	78-79	0.5197076656372523
1105	80-81	0.5501812798928256
1105	82-83	0.6020592011675845
1105	84-85	0.5225493459249613
1105	86-87	0.5531383864730941
1105	88-89	0.591654862540981
1105	90-91	0.559222339737083
1105	92-93	0.47154288692044943
1105	94-95	0.462118506145373
1105	96-97	0.5340411784498045
1105	98-99	0.511821643176404
1105	100	0.4758861669740746
1106	1	0.32265490650725326
1106	2	0.35696379081251095
1106	3	0.3435719806236328
1106	4	0.3286102824854211
1106	5	0.2783414106943667
1106	6	0.34434477185585166
1106	7	0.29722989413656364
1106	8	0.395520544143686
1106	9	0.46094337168326405
1106	10-11	0.4369048611215405
1106	12-13	0.31217432071591844
1106	14-15	0.42156286217444716
1106	16-17	0.3676448658628999
1106	18-19	0.38261677012757644
1106	20-21	0.40318175395304223
1106	22-23	0.46930934513869715
1106	24-25	0.4285850955642516
1106	26-27	0.4090618344137198
1106	28-29	0.5082318886519488
1106	30-31	0.4954451668112796
1106	32-33	0.5009941330487919
1106	34-35	0.4227342650104404
1106	36-37	0.491593652377901
1106	38-39	0.4789076919458708
1106	40-41	0.42691249364012407
1106	42-43	0.4641047147733417
1106	44-45	0.3765993759058617
1106	46-47	0.3664530301524067
1106	48-49	0.3091829142142757
1106	50-51	0.4261267073083843
1106	52-53	0.4417445516607543
1106	54-55	0.526561096990072
1106	56-57	0.45871782911510905
1106	58-59	0.47428998998280747
1106	60-61	0.3953235881616308
1106	62-63	0.5074600221968026
1106	64-65	0.5162276942530113
1106	66-67	0.48383811908941254
1106	68-69	0.4971226694779389
1106	70-71	0.4328665612763345
1106	72-73	0.41062005303760074
1106	74-75	0.4333222378813275
1106	76-77	0.42625128158969616
1106	78-79	0.37605149387628245
1106	80-81	0.35833371432955374
1106	82-83	0.37720686588075836
1106	84-85	0.4231247650068326
1106	86-87	0.44452249484349693
1106	88-89	0.4391901699095442
1106	90-91	0.39047760656489316
1106	92-93	0.4392422389065551
1106	94-95	0.45860143497858985
1106	96-97	0.46862239247226967
1106	98-99	0.5088934116375121
1106	100	0.45279241995504194
1107	1	0.0944231017292978
1107	2	0.13102706115360618
1107	3	0.19328859807464482
1107	4	0.16830016909588608
1107	5	0.07497552448177203
1107	6	0.1532831641406105
1107	7	0.2032239794944246
1107	8	0.19263184309076564
1107	9	0.31991463526284747
1107	10-11	0.30132709242883493
1107	12-13	0.29697012358980857
1107	14-15	0.3322521164817047
1107	16-17	0.3438156966894468
1107	18-19	0.3831323696420057
1107	20-21	0.264871015709474
1107	22-23	0.33518022120069446
1107	24-25	0.4003929028509745
1107	26-27	0.42364044719980143
1107	28-29	0.38061136872607904
1107	30-31	0.34756143990608024
1107	32-33	0.35705269755840163
1107	34-35	0.3646277333870316
1107	36-37	0.30520355153803536
1107	38-39	0.3357025559320306
1107	40-41	0.28014585032880035
1107	42-43	0.31893197506009585
1107	44-45	0.32039274815892327
1107	46-47	0.29823833802008437
1107	48-49	0.33643614616877215
1107	50-51	0.2857663514483164
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2216	3	-1.1020773951243363
2216	4	-0.9674624185078855
2216	5	-1.171540616700689
2216	6	-1.068006432031197
2216	7	-1.0711718152431402
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2216	9	-1.2883409106808088
2216	10-11	-1.5657327642860395
2216	12-13	-1.519704889755964
2216	14-15	-1.8976135869059334
2216	16-17	-1.6981064867379985
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2216	36-37	-1.8898394594295667
2216	38-39	-1.9227309070539889
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2216	54-55	-2.0381772676095053
2216	56-57	-1.800923409427007
2216	58-59	-1.951986101102861
2216	60-61	-1.8310415939127083
2216	62-63	-1.8580733807728436
2216	64-65	-1.8012353411404902
2216	66-67	-1.8883209469486388
2216	68-69	-1.8596387901151168
2216	70-71	-1.9322645017531102
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2216	74-75	-1.9009979912947976
2216	76-77	-1.6841266455885773
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2216	80-81	-1.6276639230712675
2216	82-83	-1.736841472703908
2216	84-85	-1.7712472822806298
2216	86-87	-1.7855225970984598
2216	88-89	-1.7400612140191143
2216	90-91	-1.8813933585560143
2216	92-93	-1.7720076298458842
2216	94-95	-1.7001686630644102
2216	96-97	-1.77413747698683
2216	98-99	-1.9899821651007557
2216	100	-1.9632268493329121
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	150961.0
3	32737.0
4	21763.0
5	19347.0
6	14128.0
7	12864.0
8	14261.0
9	14965.0
10	15156.0
11	15674.0
12	17124.0
13	18392.0
14	19153.0
15	19069.0
16	18261.0
17	18058.0
18	17472.0
19	16736.0
20	16595.0
21	17999.0
22	22308.0
23	34400.0
24	51429.0
25	63637.0
26	67012.0
27	55888.0
28	57927.0
29	68337.0
30	79058.0
31	92410.0
32	115100.0
33	149036.0
34	200811.0
35	299893.0
36	469842.0
37	796912.0
38	1031466.0
39	426154.0
40	1150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49962496266028	21.8812091641194	13.73979362668324	21.87937224653708
2	15.68872357686244	22.98585007773601	38.82803394581876	22.497392399582782
3	17.599398918192698	28.61412244855118	30.02483223589837	23.761646397357758
4	12.126066588863981	20.056626709149818	37.99637751751708	29.820929184469115
5	11.86973533470703	40.42714078257026	33.61528902207472	14.087834860647982
6	27.625844972848906	40.374154152438	18.509956859150197	13.490044015562896
7	24.90934317949974	35.74254621950726	22.070275299774874	17.277835301218126
8	23.52014155647838	37.867801171050985	20.73396999053389	17.878087281936743
9	24.233435770766977	20.16595389047227	21.425794378463387	34.174815960297366
10-11	22.000394250157736	30.24732910824572	28.224222287991324	19.528054353605217
12-13	22.78867273898122	28.520799918656785	27.7380792287264	20.952448113635594
14-15	20.8985182827636	30.37816081108666	25.543498404335637	23.17982250181411
16-17	19.9071411672045	33.08062299187062	26.203838990170308	20.80839685075458
18-19	19.769043947073975	31.779471540048863	28.188240706665784	20.26324380621138
20-21	21.893218314134614	30.88118325953304	27.258225003555992	19.96737342277635
22-23	21.30932641365405	31.145499347617456	27.319631586991882	20.225542651736614
24-25	20.847047134074394	30.978174830514476	27.598009031647504	20.576769003763623
26-27	20.658312313336026	30.749069939601696	27.73885253173117	20.85376521533111
28-29	20.52569098261091	30.877060275020796	27.871985573769486	20.725263168598808
30-31	21.029826837024935	31.430057394456124	27.236203726703728	20.303912041815213
32-33	20.386795528537682	30.988588250549004	27.940877739752462	20.683738481160848
34-35	20.6353111011839	30.485020948827398	27.890103988662858	20.989563961325846
36-37	21.06891696600126	30.96400353450615	27.346732560730462	20.620346938762122
38-39	20.878119232754024	30.840924596128783	27.691232036805413	20.58972413431178
40-41	20.60851274537126	30.553445761803673	27.93588627427528	20.90215521854979
42-43	20.517942225400095	30.669179081142893	27.869717424436235	20.94316126902077
44-45	20.934473542484408	30.74228682795164	27.61876346534777	20.70447616421618
46-47	20.723760347317153	31.10966622799024	27.50092303541056	20.665650389282053
48-49	20.50161818312616	30.598518937695196	27.82953548818489	21.070327390993757
50-51	20.58807097810991	30.421692048816556	27.79799950823531	21.19223746483822
52-53	20.591031226483985	30.559477738998726	27.706664628771215	21.142826405746078
54-55	20.848910244846415	30.926097818303077	27.447660593338476	20.777331343512035
56-57	20.475503267287873	30.64755382524684	27.490972183501643	21.385970723963645
58-59	20.483916303796313	30.181637253113045	27.90385478028418	21.43059166280646
60-61	20.82001610640108	30.6894943451576	27.338680705139378	21.151808843301946
62-63	20.583255354364532	30.57950864667322	27.56421252393077	21.273023475031476
64-65	20.629929093754665	30.20420189611751	27.787223318867092	21.37864569126073
66-67	20.411234518055306	30.275411806855434	27.49549209152194	21.817861583567325
68-69	20.934780911714615	30.499357252982996	27.420162817582515	21.145699017719878
70-71	20.955326736249784	30.143593765835856	27.523495973953942	21.37758352396041
72-73	20.58998358866869	30.243423632645065	27.93609606231911	21.23049671636713
74-75	20.637647948886706	30.215289819104953	27.634774794079643	21.512287437928705
76-77	20.628900975756416	30.222406865826663	27.389324076857903	21.759368081559025
78-79	20.83233342491492	30.28283186308986	27.58520559009939	21.299629121895826
80-81	20.492068896074038	30.16963813839521	27.752752211029048	21.585540754501707
82-83	20.398687237606865	30.20869878369737	27.927173370326884	21.465440608368876
84-85	20.520893441230356	29.920170517471462	27.74783513175068	21.811100909547505
86-87	20.89413359099851	30.09661132244291	27.4869860936459	21.52226899291268
88-89	20.622469953762067	29.90362004539502	27.669969199128136	21.803940801714784
90-91	20.457328602629275	29.755702859511846	27.61500205919663	22.171966478662252
92-93	20.638143393617387	29.943084720884013	27.50124552395806	21.91752636154054
94-95	20.528177409989688	29.738456443790863	27.744002200547886	21.98936394567157
96-97	20.525950694582242	29.800745938635803	27.64060691832666	22.03269644845529
98-99	20.43318809183723	29.755308803536963	27.67673189688018	22.13477120774563
100	20.637446644936524	29.849207510171382	27.391217550467694	22.1221282944244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37278.0
1	27595.5
2	15385.5
3	10596.0
4	6980.5
5	4958.0
6	4082.0
7	3926.0
8	4115.5
9	4061.5
10	3460.0
11	2927.0
12	2751.0
13	2796.5
14	2949.5
15	3042.0
16	3247.5
17	3759.5
18	4530.5
19	5484.5
20	6822.5
21	8910.5
22	12187.0
23	17443.5
24	23948.5
25	31372.0
26	41873.5
27	55447.5
28	71245.0
29	85428.0
30	96079.0
31	106710.0
32	117071.5
33	126733.0
34	135775.5
35	143262.5
36	147796.5
37	150441.0
38	152424.5
39	152231.0
40	151149.5
41	148714.5
42	142980.0
43	137928.5
44	135343.0
45	133610.0
46	133057.5
47	131437.0
48	130531.5
49	132112.0
50	132812.0
51	129089.0
52	123038.0
53	117896.5
54	114268.0
55	111375.0
56	102192.5
57	90396.5
58	83010.0
59	76069.0
60	65121.5
61	53231.0
62	43483.0
63	33940.0
64	25875.5
65	19545.5
66	14439.0
67	10777.5
68	8019.0
69	5872.0
70	4229.0
71	3193.5
72	2467.5
73	1865.0
74	1424.5
75	1063.5
76	714.5
77	539.0
78	444.0
79	341.0
80	255.5
81	170.0
82	136.0
83	135.5
84	116.0
85	84.0
86	70.5
87	61.5
88	52.0
89	39.5
90	31.5
91	29.5
92	32.5
93	26.5
94	20.5
95	15.0
96	11.5
97	15.5
98	20.0
99	22.5
100	32.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013272154604202266
2	0.006887526689165921
3	0.008680470144758319
4	0.008265032026999105
5	0.009314559903443435
6	0.012178896399572755
7	0.010276627123517405
8	0.007171773822369594
9	0.006275302094573394
10-11	0.0053897629488234905
12-13	0.005903594304999361
14-15	0.006810998614841855
16-17	0.004351167654425455
18-19	0.005116448397666112
20-21	0.004799403518323554
22-23	0.005422560694962376
24-25	0.005094583233573522
26-27	0.005695875246119753
28-29	0.015382142939137224
30-31	0.00483220126446244
32-33	0.004722875443999488
34-35	0.004329302490332864
36-37	0.006253436930480805
38-39	0.005356965202684605
40-41	0.005171111307897588
42-43	0.004263706998055093
44-45	0.0037608082239255184
46-47	0.0056521449179345725
48-49	0.003192313957518173
50-51	0.003487493672768141
52-53	0.003050190390916336
54-55	0.004591684459443947
56-57	0.002623819691110827
58-59	0.00243796579632381
60-61	0.003236044285703353
62-63	0.0029845948986385656
64-65	0.004209044087823618
66-67	0.002918999406360795
68-69	0.003192313957518173
70-71	0.00440583056465693
72-73	0.002634752273157122
74-75	0.002514493870647876
76-77	0.002405168050184925
78-79	0.003268842031842238
80-81	0.0035421565829996163
82-83	0.001650819888990562
84-85	0.0024488983783701053
86-87	0.002361437721999744
88-89	0.001650819888990562
90-91	0.0021755838272127276
92-93	0.002372370304046039
94-95	0.0017164153812683327
96-97	0.0011479211148609867
98-99	0.0014758985762498402
100	0.0024926287065552854
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4573485.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.25899142258915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.33966068977017	50.471082535796675
2	14.752525383070292	19.25469854870626
3	4.271453987116354	8.36252337321631
4	1.5624898727475105	4.078660528140487
5	0.7599174790210964	2.4795724122656653
6	0.3761928304700108	1.4729978818129181
7	0.2156699402087174	0.9852081944733703
8	0.14602474756392256	0.7623542199007814
9	0.0997791594096371	0.5860338577259996
>10	0.4218205706164011	4.786491724479116
>50	0.02978915163856685	1.3330501273893502
>100	0.022229468675883244	2.95193242369659
>500	0.0017763859527500506	0.7812479231020661
>1k	6.033003648661412E-4	0.553159365370607
>5k	3.3516686936816026E-5	0.13014227852673532
>10k+	3.3516686936816026E-5	1.0108446053971551
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46215	1.0104985585390571	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5950	0.13009772635091185	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.1865164092590225E-5	0.0
10-11	0.0	0.0	0.0	2.1865164092590225E-5	0.0
12-13	0.0	0.0	0.0	2.1865164092590225E-5	0.0
14-15	0.0	0.0	0.0	7.652807432406578E-5	0.0
16-17	0.0	0.0	0.0	1.4212356660183643E-4	0.0
18-19	0.0	0.0	0.0	1.749213127407218E-4	0.0
20-21	0.0	0.0	0.0	3.2797746138885336E-4	0.0
22-23	0.0	0.0	0.0	6.88752668916592E-4	0.0
24-25	0.0	0.0	0.0	0.001760145709453513	0.0
26-27	0.0	0.0	0.0	0.0034546959266292553	0.0
28-29	0.0	0.0	0.0	0.0078277287451473	0.0
30-31	0.0	0.0	0.0	0.01751399643816477	0.0
32-33	2.1865164092590225E-5	0.0	0.0	0.036416430796209015	0.0
34-35	2.1865164092590225E-5	0.0	0.0	0.06707139085402052	0.0
36-37	2.1865164092590225E-5	0.0	0.0	0.11297730286641368	0.0
38-39	2.1865164092590225E-5	0.0	0.0	0.17527115536620325	0.0
40-41	2.1865164092590225E-5	0.0	0.0	0.24249560236887188	0.0
42-43	2.1865164092590225E-5	0.0	0.0	0.3134371272672809	0.0
44-45	2.1865164092590225E-5	0.0	0.0	0.4010617723683362	0.0
46-47	2.1865164092590225E-5	0.0	0.0	0.5056537848052416	0.0
48-49	2.1865164092590225E-5	0.0	0.0	0.6187404134921182	0.0
50-51	2.1865164092590225E-5	0.0	0.0	0.7362110075795592	0.0
52-53	2.1865164092590225E-5	0.0	0.0	0.8526320737905557	0.0
54-55	2.1865164092590225E-5	0.0	0.0	0.9850584401173286	0.0
56-57	2.1865164092590225E-5	0.0	0.0	1.1456471377953572	0.0
58-59	2.1865164092590225E-5	0.0	0.0	1.32387009031406	0.0
60-61	2.1865164092590225E-5	0.0	0.0	1.5096037266985678	0.0
62-63	2.1865164092590225E-5	0.0	0.0	1.6965618122722605	0.0
64-65	2.1865164092590225E-5	0.0	0.0	1.9014056020736922	0.0
66-67	2.1865164092590225E-5	0.0	0.0	2.1423925081201753	0.0
68-69	2.1865164092590225E-5	0.0	0.0	2.408688341603832	0.0
70-71	2.1865164092590225E-5	0.0	0.0	2.7031027761105593	0.0
72-73	2.1865164092590225E-5	0.0	0.0	3.0208254755399877	0.0
74-75	2.1865164092590225E-5	0.0	0.0	3.343161724592953	0.0
76-77	2.1865164092590225E-5	0.0	0.0	3.686138688549323	0.0
78-79	2.1865164092590225E-5	0.0	0.0	4.076792642809586	0.0
80-81	2.1865164092590225E-5	0.0	0.0	4.502900960645984	0.0
82-83	2.1865164092590225E-5	0.0	0.0	4.948720723911853	0.0
84-85	2.1865164092590225E-5	0.0	0.0	5.387620162742417	0.0
86-87	2.1865164092590225E-5	0.0	0.0	5.859350145458004	2.1865164092590225E-5
88	2.1865164092590225E-5	0.0	0.0	6.238043854959621	2.1865164092590225E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7130	0.0	45.623634	1
GTATCAA	11500	0.0	33.314484	1
TACCTGG	4320	0.0	32.315643	2
GTACCTG	4895	0.0	29.290077	1
ATCAACG	13065	0.0	28.062126	3
TCAACGC	13065	0.0	28.062126	4
TATCAAC	13925	0.0	26.903154	2
TCTGTCG	1255	0.0	25.840609	8
CAACGCA	14615	0.0	25.085983	5
GTACATG	21930	0.0	24.650887	1
CATGGGT	10465	0.0	24.523855	4
AACGCAG	15335	0.0	23.999065	6
TACATGG	21850	0.0	23.792704	2
ACCTGGG	6015	0.0	22.661972	3
CGCAGAG	15910	0.0	22.39218	8
GTCTCGC	1165	0.0	22.192698	1
ACATGGG	22460	0.0	22.120789	3
ACGCAGA	16425	0.0	22.062555	7
CTGTCGC	1465	0.0	21.174038	9
GTGGTAT	3945	0.0	20.73364	1
>>END_MODULE