##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431457_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4573485
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.255969135134364	33.0	31.0	34.0	30.0	34.0
2	32.34850841316852	34.0	31.0	34.0	30.0	34.0
3	32.45755173571139	34.0	31.0	34.0	30.0	34.0
4	36.01937647111557	37.0	35.0	37.0	35.0	37.0
5	35.94417058326418	37.0	35.0	37.0	35.0	37.0
6	35.99020418783488	37.0	35.0	37.0	35.0	37.0
7	35.92210054258405	37.0	35.0	37.0	35.0	37.0
8	35.905536806177345	37.0	35.0	37.0	35.0	37.0
9	37.577977625377585	39.0	37.0	39.0	35.0	39.0
10-11	37.51378522067964	39.0	37.0	39.0	34.5	39.0
12-13	37.50234602278131	39.0	37.0	39.0	34.0	39.0
14-15	38.80713733618893	40.0	38.0	41.0	34.0	41.0
16-17	38.86079543280452	40.0	38.0	41.0	35.0	41.0
18-19	38.96676440395016	40.0	38.5	41.0	35.0	41.0
20-21	38.98984603644704	40.0	39.0	41.0	35.0	41.0
22-23	38.905347891159586	40.0	38.0	41.0	35.0	41.0
24-25	38.8412237057736	40.0	38.0	41.0	35.0	41.0
26-27	38.68480808398847	40.0	38.0	41.0	34.5	41.0
28-29	38.57834747462821	40.0	38.0	41.0	34.0	41.0
30-31	38.35750100853069	40.0	38.0	41.0	34.0	41.0
32-33	38.0947866889254	40.0	37.0	41.0	33.5	41.0
34-35	37.910552128191085	40.0	37.0	41.0	33.0	41.0
36-37	37.74204933436974	40.0	37.0	41.0	33.0	41.0
38-39	37.615708371187395	40.0	37.0	41.0	33.0	41.0
40-41	37.46571771854505	40.0	37.0	41.0	33.0	41.0
42-43	37.306901520394185	40.0	36.5	41.0	32.5	41.0
44-45	37.043158226166696	40.0	36.0	41.0	31.5	41.0
46-47	36.64881780524043	40.0	36.0	41.0	30.5	41.0
48-49	36.33385448951948	40.0	36.0	41.0	30.0	41.0
50-51	36.173953232600525	40.0	36.0	41.0	29.5	41.0
52-53	36.03098118830607	40.0	35.0	41.0	29.0	41.0
54-55	35.877506321765566	40.0	35.0	41.0	28.0	41.0
56-57	35.65733614519343	39.0	35.0	41.0	28.0	41.0
58-59	35.49302665254177	39.0	35.0	41.0	28.0	41.0
60-61	35.28712305823677	39.0	35.0	41.0	27.0	41.0
62-63	35.0608925141331	38.5	35.0	41.0	27.0	41.0
64-65	34.818593260937774	38.0	35.0	40.5	26.0	41.0
66-67	34.56525220920152	37.0	35.0	40.0	26.0	41.0
68-69	34.27796723942464	37.0	34.0	40.0	26.0	41.0
70-71	33.953161101435775	36.5	34.0	39.5	26.0	41.0
72-73	33.63105738840294	36.0	34.0	39.0	25.5	41.0
74-75	33.328691905625575	35.5	34.0	39.0	25.0	41.0
76-77	32.9772537791203	35.0	34.0	38.5	24.0	40.0
78-79	32.66265517433642	35.0	33.5	37.0	24.0	39.0
80-81	32.35674556711129	35.0	33.0	37.0	23.5	39.0
82-83	32.096416955560144	35.0	33.0	36.0	23.0	39.0
84-85	31.86057273610824	35.0	33.0	36.0	22.5	37.0
86-87	31.609537256599726	35.0	33.0	36.0	21.5	37.0
88-89	31.453774528614396	35.0	33.0	35.0	21.5	37.0
90-91	31.259740110659596	35.0	33.0	35.0	20.0	36.0
92-93	31.062970251350993	35.0	33.0	35.0	19.5	36.0
94-95	30.895554702814156	35.0	33.0	35.0	17.5	36.0
96-97	30.725625753664875	35.0	33.0	35.0	8.0	36.0
98-99	30.558932739475477	35.0	33.0	35.0	2.0	35.5
100	30.44248598169667	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17557474350715552
1101	2	0.2541513085477405
1101	3	0.24807652485426246
1101	4	0.19868570425867915
1101	5	0.23039720899113547
1101	6	0.25760198199437667
1101	7	0.23848016707412256
1101	8	0.28820147888595216
1101	9	0.3951072015934116
1101	10-11	0.3596130024465367
1101	12-13	0.39152385788612065
1101	14-15	0.48577797470752415
1101	16-17	0.4590912735287347
1101	18-19	0.44302813490526916
1101	20-21	0.413094768663143
1101	22-23	0.4196141899423296
1101	24-25	0.39114642952267076
1101	26-27	0.4032973930018926
1101	28-29	0.3952096638647262
1101	30-31	0.468388905189137
1101	32-33	0.4079114693663968
1101	34-35	0.4782532282187546
1101	36-37	0.5336624112322781
1101	38-39	0.5127345858838055
1101	40-41	0.6411613821182485
1101	42-43	0.6647008578664639
1101	44-45	0.6452595130125829
1101	46-47	0.6595636488136378
1101	48-49	0.7461599595574313
1101	50-51	0.6629302587201238
1101	52-53	0.6552572166110338
1101	54-55	0.6035209428468278
1101	56-57	0.6652737205595614
1101	58-59	0.6542899322112419
1101	60-61	0.6659118399819732
1101	62-63	0.6669985324299645
1101	64-65	0.6355435854032621
1101	66-67	0.5723735944407835
1101	68-69	0.587581225986213
1101	70-71	0.592931553716042
1101	72-73	0.6637041138114057
1101	74-75	0.6215717642723888
1101	76-77	0.6599845553032395
1101	78-79	0.6093403393894121
1101	80-81	0.6348194256911128
1101	82-83	0.565277764064767
1101	84-85	0.5797808469095074
1101	86-87	0.6020809594622278
1101	88-89	0.5414288967714427
1101	90-91	0.6166649600669025
1101	92-93	0.6457083808924473
1101	94-95	0.6355625936966973
1101	96-97	0.6903783926261617
1101	98-99	0.7854813999588295
1101	100	0.8167091957384969
1102	1	0.18656849886616556
1102	2	0.21292904134975288
1102	3	0.22418558963400415
1102	4	0.18870459605846435
1102	5	0.17924878382216747
1102	6	0.1458316824597432
1102	7	0.1770081716102112
1102	8	0.21841498451471608
1102	9	0.26592386190939976
1102	10-11	0.29636929486603947
1102	12-13	0.2896729650116683
1102	14-15	0.43054619355247326
1102	16-17	0.38896241598307313
1102	18-19	0.34923739210479
1102	20-21	0.31236340633665804
1102	22-23	0.32925615482404424
1102	24-25	0.30574259529252856
1102	26-27	0.35334936572995446
1102	28-29	0.3258911861849114
1102	30-31	0.33372871918730596
1102	32-33	0.34131582253246506
1102	34-35	0.4199661203437799
1102	36-37	0.37797367267587134
1102	38-39	0.4650010380121188
1102	40-41	0.4597394907778565
1102	42-43	0.4549014514278582
1102	44-45	0.4908697711668779
1102	46-47	0.46881362220408107
1102	48-49	0.3761746502118797
1102	50-51	0.3382463684456951
1102	52-53	0.2871792885799067
1102	54-55	0.29275919517036186
1102	56-57	0.3787216787851193
1102	58-59	0.3171118139365845
1102	60-61	0.2938013268111277
1102	62-63	0.2956734959382672
1102	64-65	0.3009827747394951
1102	66-67	0.2606772477259085
1102	68-69	0.3066455328892346
1102	70-71	0.293593815263705
1102	72-73	0.2424638173986864
1102	74-75	0.2244375794726352
1102	76-77	0.22086058066166459
1102	78-79	0.2500254396570014
1102	80-81	0.2357678933562326
1102	82-83	0.21532008217109677
1102	84-85	0.15747307675914612
1102	86-87	0.19408626495384596
1102	88-89	0.21836292226993237
1102	90-91	0.16090498899911054
1102	92-93	0.1622947193257147
1102	94-95	0.2529143477713234
1102	96-97	0.2339103747477047
1102	98-99	0.30177520632114607
1102	100	0.3238882379287986
1103	1	0.1454686406355421
1103	2	0.17817555400751672
1103	3	0.14680633962785805
1103	4	0.1584294527514558
1103	5	0.12324449691292472
1103	6	0.11095576142005825
1103	7	0.12151860595960784
1103	8	0.15338712267989507
1103	9	0.24173542241816648
1103	10-11	0.22673296715427682
1103	12-13	0.23779404021401973
1103	14-15	0.352689029278352
1103	16-17	0.3117741202601394
1103	18-19	0.29116278404638507
1103	20-21	0.24611745414461694
1103	22-23	0.22199106991617867
1103	24-25	0.28947813389316224
1103	26-27	0.24414062184972352
1103	28-29	0.2401658789666712
1103	30-31	0.25133583810136884
1103	32-33	0.28952021799830163
1103	34-35	0.2620137729995804
1103	36-37	0.2747916650861342
1103	38-39	0.2089620006907893
1103	40-41	0.18133785212678077
1103	42-43	0.23619979063494156
1103	44-45	0.21370515492828446
1103	46-47	0.2303883156008837
1103	48-49	0.22562444816745142
1103	50-51	0.2523265511775037
1103	52-53	0.2862996687740207
1103	54-55	0.26083629034343403
1103	56-57	0.3226393572115782
1103	58-59	0.23773739439147334
1103	60-61	0.24484668378703844
1103	62-63	0.22449867445697436
1103	64-65	0.26136038563328867
1103	66-67	0.25057915853727764
1103	68-69	0.24004323521043602
1103	70-71	0.25961894473205405
1103	72-73	0.2360078904545091
1103	74-75	0.22236956476675118
1103	76-77	0.24047524702695
1103	78-79	0.26092953467016855
1103	80-81	0.22769726391248923
1103	82-83	0.19167259973081485
1103	84-85	0.22235226910421346
1103	86-87	0.2343816549887805
1103	88-89	0.17910024221359677
1103	90-91	0.22708001806029898
1103	92-93	0.26065366571167203
1103	94-95	0.27489753918151294
1103	96-97	0.2397050251831594
1103	98-99	0.27119638131561175
1103	100	0.21323585275892043
1104	1	0.13054341443966422
1104	2	0.17157869932734116
1104	3	0.141783877558197
1104	4	0.13942490363103133
1104	5	0.06529834287290726
1104	6	0.121668955759489
1104	7	0.12818398097851258
1104	8	0.13479684595223773
1104	9	0.17971427719915312
1104	10-11	0.21413506970480967
1104	12-13	0.21704911386944303
1104	14-15	0.29517624669102815
1104	16-17	0.2979253328338629
1104	18-19	0.26396395062484146
1104	20-21	0.28816117957180154
1104	22-23	0.26255373215973776
1104	24-25	0.2798463007614558
1104	26-27	0.27353072782784693
1104	28-29	0.3147148261162229
1104	30-31	0.3237638051108007
1104	32-33	0.2864245951090396
1104	34-35	0.32171192121773373
1104	36-37	0.24860468804596536
1104	38-39	0.2917077918092872
1104	40-41	0.284387912928743
1104	42-43	0.24500366967370013
1104	44-45	0.2922564380700763
1104	46-47	0.3147183442061632
1104	48-49	0.26753881192065876
1104	50-51	0.23844330668433855
1104	52-53	0.19794328267845174
1104	54-55	0.20289525563907063
1104	56-57	0.22582011859068274
1104	58-59	0.21861411606343495
1104	60-61	0.22478755152231145
1104	62-63	0.22766537735278547
1104	64-65	0.2537475397237188
1104	66-67	0.27721801539750857
1104	68-69	0.3366976706028453
1104	70-71	0.3298046427259038
1104	72-73	0.32356782606114365
1104	74-75	0.27338472902654587
1104	76-77	0.27185618388112687
1104	78-79	0.2973555768093874
1104	80-81	0.2658000853752682
1104	82-83	0.28016208855333957
1104	84-85	0.3132407573722631
1104	86-87	0.3089800234171278
1104	88-89	0.20308728320608438
1104	90-91	0.219715853724896
1104	92-93	0.23201551138181742
1104	94-95	0.2098731160125027
1104	96-97	0.2510776293340733
1104	98-99	0.2949280462180752
1104	100	0.3868679662727068
1105	1	0.10595137736579119
1105	2	0.1767032285150023
1105	3	0.13648685078683798
1105	4	0.10305401698943228
1105	5	0.09580922422145477
1105	6	0.11372062437941821
1105	7	0.15516764959951246
1105	8	0.18896832208227465
1105	9	0.228706717138067
1105	10-11	0.1838199089156234
1105	12-13	0.14487434963400858
1105	14-15	0.2093765737955664
1105	16-17	0.20270734769317755
1105	18-19	0.23710580888626254
1105	20-21	0.239972042458092
1105	22-23	0.18312649961227123
1105	24-25	0.22392246131402516
1105	26-27	0.24782369763467216
1105	28-29	0.2554643288989098
1105	30-31	0.20722208177486579
1105	32-33	0.21885899980458134
1105	34-35	0.1841764157442114
1105	36-37	0.21864251648694477
1105	38-39	0.22909210677929082
1105	40-41	0.19045270690656224
1105	42-43	0.23580750528754635
1105	44-45	0.3078221957567209
1105	46-47	0.23626124030236895
1105	48-49	0.19414073278230148
1105	50-51	0.12818803169706428
1105	52-53	0.08684014578142296
1105	54-55	0.06690708140351376
1105	56-57	0.15122364458866144
1105	58-59	0.13012684149488507
1105	60-61	0.06394808025230958
1105	62-63	0.10009924970626827
1105	64-65	0.09079321458294487
1105	66-67	0.07832960769066233
1105	68-69	0.08659198373376853
1105	70-71	0.11061122624300879
1105	72-73	0.0939137318371337
1105	74-75	0.08219747315249037
1105	76-77	0.11082704342329919
1105	78-79	0.07455838993347896
1105	80-81	0.07565884591682703
1105	82-83	0.0724049604172663
1105	84-85	0.08673522972348735
1105	86-87	0.07184445777824067
1105	88-89	0.10376810939738235
1105	90-91	0.08204143466326386
1105	92-93	0.09880431378099885
1105	94-95	0.14132141510370744
1105	96-97	0.10707588725362527
1105	98-99	0.15071107851167653
1105	100	0.18267387900308663
1106	1	0.12094645599716358
1106	2	0.15022110005467937
1106	3	0.13220900604610364
1106	4	0.1231121530486945
1106	5	0.10246122182919493
1106	6	0.08015528182454545
1106	7	0.07890471407257849
1106	8	0.09037850944866932
1106	9	0.18963897221729553
1106	10-11	0.1758398557048082
1106	12-13	0.16673103454539273
1106	14-15	0.23412957854852579
1106	16-17	0.22918593877194837
1106	18-19	0.1943681650137492
1106	20-21	0.20496311492421881
1106	22-23	0.1784651876596044
1106	24-25	0.1852649558849535
1106	26-27	0.24040827072884952
1106	28-29	0.17060104041656388
1106	30-31	0.2469654450664791
1106	32-33	0.16006255764057897
1106	34-35	0.19849554269521974
1106	36-37	0.19962458563967544
1106	38-39	0.2381058065311663
1106	40-41	0.2744709912769352
1106	42-43	0.2764564275022252
1106	44-45	0.31398323921245463
1106	46-47	0.23790019841211318
1106	48-49	0.23105436287033143
1106	50-51	0.19068296216698144
1106	52-53	0.2121959590561886
1106	54-55	0.2413952857305901
1106	56-57	0.23416327488262567
1106	58-59	0.2399059940561159
1106	60-61	0.22488642521878432
1106	62-63	0.21373129889054354
1106	64-65	0.1967341146355892
1106	66-67	0.2189747688838679
1106	68-69	0.1832106254463497
1106	70-71	0.17454247339428264
1106	72-73	0.24897009942066006
1106	74-75	0.2716652625948157
1106	76-77	0.2661504779373587
1106	78-79	0.1934287958425287
1106	80-81	0.19128898882875234
1106	82-83	0.1890545465844795
1106	84-85	0.19799956644057914
1106	86-87	0.20614883567603925
1106	88-89	0.16623276246175678
1106	90-91	0.19106628630612832
1106	92-93	0.18307853684514086
1106	94-95	0.2713516684249555
1106	96-97	0.26283043073528845
1106	98-99	0.2540261340117951
1106	100	0.23341709039158687
1107	1	0.05947969076773063
1107	2	0.09219176229624537
1107	3	0.061544997126965484
1107	4	0.12301166226514937
1107	5	0.08872874728308489
1107	6	0.04782806864284339
1107	7	-0.0030142626040614573
1107	8	0.03252400812615264
1107	9	0.05438889403750835
1107	10-11	0.10684175904113857
1107	12-13	-0.009098697259609878
1107	14-15	0.06269763308347365
1107	16-17	0.09921038519992464
1107	18-19	0.10138249473657623
1107	20-21	0.023608507925700906
1107	22-23	0.012140744693311945
1107	24-25	0.03459063789676975
1107	26-27	0.019003704620445205
1107	28-29	0.029876989714871627
1107	30-31	0.025101221260655393
1107	32-33	0.07304966224842246
1107	34-35	0.08406735935387388
1107	36-37	0.08844153890226636
1107	38-39	0.03317457187773698
1107	40-41	0.05109838328257865
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2216	72-73	-1.0043735162830387
2216	74-75	-0.9397651055306397
2216	76-77	-0.8999515738733166
2216	78-79	-0.8558158619167564
2216	80-81	-0.8855333353016519
2216	82-83	-0.8804613723501546
2216	84-85	-0.8872853144409945
2216	86-87	-0.9482052789743918
2216	88-89	-0.8953759449950809
2216	90-91	-1.0039705388274847
2216	92-93	-0.9533803338164297
2216	94-95	-1.083640473887172
2216	96-97	-1.31959512722063
2216	98-99	-1.3477946237308096
2216	100	-1.386548903972848
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	278.0
3	898.0
4	596.0
5	528.0
6	895.0
7	1756.0
8	3082.0
9	4750.0
10	6699.0
11	9232.0
12	12762.0
13	18276.0
14	19372.0
15	24597.0
16	31875.0
17	45718.0
18	51041.0
19	28514.0
20	18220.0
21	16660.0
22	16308.0
23	16873.0
24	19135.0
25	22575.0
26	27755.0
27	35402.0
28	44683.0
29	56335.0
30	70490.0
31	89423.0
32	111700.0
33	141922.0
34	189995.0
35	292278.0
36	436217.0
37	820144.0
38	1244878.0
39	637518.0
40	4105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.82238433135307	21.1143338972176	14.655427191005385	23.407854580423948
2	14.421059651447418	22.024101970379263	39.60679875412295	23.94803962405037
3	16.270760996003045	27.714082055677515	31.827579873531754	24.18757707478769
4	11.065260366468033	18.835502917150308	38.45172780848878	31.64750890789288
5	11.136339782388132	38.16921334359584	35.12734842388833	15.567098450127698
6	27.398931012127512	39.27722513575534	18.5242763450629	14.799567507054249
7	24.077765642611705	34.601818963000866	23.229856444265153	18.090558950122283
8	24.844161509221085	34.18436925014513	21.49439650507217	19.477072735561613
9	25.051727511952045	15.848526889232172	23.118409702885216	35.98133589593056
10-11	22.626616636748288	27.177199110342737	29.37472952639155	20.821454726517423
12-13	24.43651832246088	25.54314707493301	29.684813659605314	20.335520943000798
14-15	21.750940475370534	26.849098663273192	28.995361305437754	22.404599555918516
16-17	18.98809113837697	29.893593178943412	29.627756513905695	21.49055916877392
18-19	18.68887730035192	27.06044733939217	34.29329056507237	19.957384795183543
20-21	20.519275782034928	26.31579637847287	34.00162020865926	19.163307630832943
22-23	19.996534371491325	26.557373643949855	34.14764670705163	19.298445277507195
24-25	19.61330388226687	26.42700431314396	34.321548970998336	19.638142833590834
26-27	19.444045405199756	26.261352119882318	34.4437010288653	19.850901446052628
28-29	19.35950411416097	26.410580408057598	34.49461372016708	19.735301757614344
30-31	19.887829651683507	26.9380702650508	33.906225947409666	19.26787413585603
32-33	19.260523553294988	26.575709651230493	34.50109861661112	19.6626681788634
34-35	19.500906107655386	26.2072616307139	34.22537416298743	20.066458098643285
36-37	20.064951045642083	26.757167514287577	33.459005064296704	19.718876375773636
38-39	19.899606792907797	26.68116640140184	33.67377171161082	19.74545509407955
40-41	19.651069939069906	26.540351044444026	33.77213159990362	20.036447416582448
42-43	19.664604197859255	26.67099604725366	33.50989771838363	20.15450203650346
44-45	20.181305940655758	26.785984867119932	32.96743074482588	20.065278447398427
46-47	20.090089262755757	27.36541711053154	32.28909203177285	20.255401594939855
48-49	20.135982251529448	27.087572233259927	32.000543570415815	20.775901944794807
50-51	20.25214808993744	27.090242134803393	31.68975057743942	20.967859197819752
52-53	20.28989737716374	27.410545571107654	31.369398479833364	20.93015857189524
54-55	20.51428072897489	27.879038867057503	31.084549996982574	20.52213040698503
56-57	20.192396630025	27.392668911479884	31.20252103063735	21.21241342785777
58-59	20.073941446002014	26.9139297427247	31.73817503222119	21.273953779052096
60-61	20.4015648199542	27.400015764960834	31.18656912798666	21.011850287098305
62-63	20.20234730394373	27.19548770825569	31.510922623983667	21.091242363816914
64-65	20.093304017668114	26.94424641076792	31.7672826549576	21.195166916606365
66-67	20.048201806939137	27.063581780176143	31.384492771368848	21.50372364151587
68-69	20.657180243748236	27.25201781544058	31.1094243217126	20.981377619098588
70-71	20.63094481012193	27.0040862805729	31.09657657433096	21.26839233497421
72-73	20.33427462864752	27.05190899281401	31.400463760130403	21.213352618408063
74-75	20.392141857199316	27.043236840278368	31.054761999771507	21.509859302750808
76-77	20.474277274332376	27.106386049150704	30.75488385771463	21.66445281880229
78-79	20.635353081924677	27.203459002675658	30.887892900272156	21.273295015127506
80-81	20.235291030800365	27.039270927968495	31.066921614479988	21.658516426751152
82-83	20.118447088287805	27.100880093505218	31.220158504511215	21.56051431369576
84-85	20.22483653499673	26.9347075821873	30.929356267995146	21.911099614820827
86-87	20.405817445558476	27.04773274647233	30.695574600113478	21.850875207855715
88-89	20.065672020352096	26.921614479986268	30.845438434804095	22.16727506485754
90-91	19.865102870130766	26.822773005705713	30.751155847236845	22.560968276926676
92-93	19.97773904236557	26.99097929409522	30.661500940859447	22.369780722679764
94-95	19.845424222447434	26.75306686257854	30.879077989760546	22.522430925213484
96-97	19.744495805917044	26.85884690979513	30.7613567562639	22.635300528023926
98-99	19.724967830736652	26.754845614867502	30.729020556623915	22.79116599777193
100	19.87596734472905	26.858429031941373	30.420404799591033	22.845198823738546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3965.0
1	2523.5
2	6978.5
3	12133.0
4	7272.0
5	3391.0
6	6555.0
7	15477.5
8	24291.5
9	25586.5
10	17213.5
11	8444.5
12	6395.0
13	6510.0
14	6972.0
15	7532.0
16	8200.0
17	9019.5
18	9936.5
19	10744.0
20	11844.5
21	13500.5
22	16329.5
23	21062.0
24	27022.5
25	33993.5
26	43632.0
27	56257.0
28	71636.5
29	85445.5
30	95388.5
31	105349.0
32	115546.0
33	124825.0
34	133412.0
35	141231.5
36	145746.0
37	148141.0
38	150128.0
39	149974.0
40	149154.0
41	147040.5
42	141574.0
43	137229.5
44	134676.0
45	132678.5
46	131365.0
47	130105.0
48	129696.5
49	130349.5
50	131495.5
51	128187.0
52	121077.5
53	115496.5
54	111063.5
55	106708.5
56	97392.5
57	85868.0
58	79538.5
59	73726.5
60	62793.0
61	50316.0
62	40753.5
63	31849.0
64	23886.5
65	17590.0
66	12749.0
67	9285.5
68	6656.5
69	4664.0
70	3170.5
71	2252.5
72	1679.0
73	1198.0
74	874.0
75	616.0
76	390.0
77	293.5
78	188.5
79	112.0
80	67.0
81	23.0
82	11.5
83	6.5
84	2.0
85	0.5
86	1.0
87	1.0
88	1.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.5
94	1.0
95	1.0
96	2.5
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04845320362917994
2	0.0
3	1.0932582046295112E-4
4	0.0677820086870297
5	0.004482358638980996
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.01067020007718403
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.028987741295752E-4
26-27	0.0
28-29	2.842471332036729E-4
30-31	0.0037826733880181084
32-33	1.3119098455554134E-4
34-35	0.0016836176351294472
36-37	0.0042527744160087986
38-39	0.0014868311582961352
40-41	0.0010276627123517405
42-43	0.0018038760376386934
44-45	0.0
46-47	4.591684459443947E-4
48-49	4.482358638980996E-4
50-51	0.0013447075916942987
52-53	8.527413996110186E-4
54-55	0.001071393040536921
56-57	0.006034785289554902
58-59	0.008899121785684222
60-61	0.0011260559507683965
62-63	0.00241610063223122
64-65	0.0010604604584906257
66-67	1.0932582046295112E-4
68-69	7.980784893795432E-4
70-71	2.2958422297219736E-4
72-73	0.0
74-75	2.733145511573778E-4
76-77	0.0
78-79	6.122245945925263E-4
80-81	0.0
82-83	0.0011041907866758064
84-85	6.340897586851165E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	1.5305614864813158E-4
94-95	0.0
96-97	3.9357295366662404E-4
98-99	4.373032818518045E-4
100	2.623819691110827E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4573485.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.34474066425478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.92564960541006	43.87098504269629
2	16.81460303712802	19.957165132215067
3	4.908935729798081	8.739585534670843
4	1.8349622372004006	4.355814323814342
5	0.8590536021376777	2.5490156617777195
6	0.474637353854236	1.6900338404428659
7	0.2658819764825803	1.104508785916075
8	0.17197929601988465	0.8164853377536928
9	0.12286346988788724	0.6562170681846431
>10	0.5504495466688968	5.654939005232515
>50	0.03669506443355908	1.5101502140824583
>100	0.03030692004608725	3.652294557937124
>500	0.002654774369687257	1.0636821516672121
>1k	0.001032411771120967	0.9174914270177779
>5k	1.106155469057043E-4	0.5013490001387171
>10k+	1.8435924484284052E-4	2.960282916452803
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42137	0.9213324193694743	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36984	0.8086612288003568	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26607	0.5817664210115481	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18740	0.40975317509514075	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10819	0.2365592103177336	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8931	0.1952777805109233	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8480	0.1854165915051651	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5501	0.12028026767333883	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	4.373032818518045E-5	0.0	0.0	0.0	0.0
10-11	4.373032818518045E-5	0.0	0.0	0.0	0.0
12-13	4.373032818518045E-5	0.0	0.0	2.1865164092590225E-5	0.0
14-15	4.373032818518045E-5	0.0	0.0	7.652807432406578E-5	0.0
16-17	4.373032818518045E-5	0.0	0.0	9.839323841665601E-5	0.0
18-19	4.373032818518045E-5	0.0	0.0	1.2025840250924623E-4	0.0
20-21	4.373032818518045E-5	0.0	0.0	2.4051680501849246E-4	0.0
22-23	4.373032818518045E-5	0.0	0.0	5.90359430499936E-4	0.0
24-25	4.373032818518045E-5	0.0	0.0	0.0016945502171757424	0.0
26-27	4.373032818518045E-5	0.0	0.0	0.003454695926629255	0.0
28-29	4.373032818518045E-5	0.0	0.0	0.007882391655378776	0.0
30-31	4.373032818518045E-5	0.0	0.0	0.017995030048201754	0.0
32-33	4.373032818518045E-5	0.0	0.0	0.03726917219582004	0.0
34-35	4.373032818518045E-5	0.0	0.0	0.06854728943027036	0.0
36-37	4.373032818518045E-5	0.0	0.0	0.11549179673706156	0.0
38-39	4.373032818518045E-5	0.0	0.0	0.17904289617217506	0.0
40-41	4.373032818518045E-5	0.0	0.0	0.24807121921248237	0.0
42-43	4.373032818518045E-5	0.0	0.0	0.3206744965819282	0.0
44-45	4.373032818518045E-5	0.0	0.0	0.4103653996897333	0.0
46-47	4.373032818518045E-5	0.0	0.0	0.5173079172665921	0.0
48-49	4.373032818518045E-5	0.0	0.0	0.6327231859293296	0.0
50-51	4.373032818518045E-5	0.0	0.0	0.7526645435592333	0.0
52-53	4.373032818518045E-5	0.0	0.0	0.8718187552818037	0.0
54-55	4.373032818518045E-5	0.0	0.0	1.007677952371113	0.0
56-57	6.559549227777068E-5	0.0	0.0	1.172978592911095	0.0
58-59	8.74606563703609E-5	0.0	0.0	1.3558806905456122	0.0
60-61	8.74606563703609E-5	0.0	0.0	1.5462934720459343	0.0
62-63	8.74606563703609E-5	0.0	0.0	1.7377885791688397	0.0
64-65	8.74606563703609E-5	0.0	0.0	1.9480002667550018	0.0
66-67	8.74606563703609E-5	0.0	0.0	2.1945518570630496	2.1865164092590225E-5
68-69	8.74606563703609E-5	0.0	0.0	2.4670901948951403	2.1865164092590225E-5
70-71	8.74606563703609E-5	0.0	0.0	2.766107246443358	2.1865164092590225E-5
72-73	8.74606563703609E-5	0.0	0.0	3.090859596128554	2.1865164092590225E-5
74-75	8.74606563703609E-5	0.0	0.0	3.41924156305312	2.1865164092590225E-5
76-77	8.74606563703609E-5	0.0	0.0	3.7692153795191192	2.1865164092590225E-5
78-79	8.74606563703609E-5	0.0	0.0	4.168571669088234	2.1865164092590225E-5
80-81	8.74606563703609E-5	0.0	0.0	4.603458850307806	2.1865164092590225E-5
82-83	1.0932582046295112E-4	0.0	0.0	5.059445914876729	2.1865164092590225E-5
84-85	1.0932582046295112E-4	0.0	0.0	5.508698508905135	2.1865164092590225E-5
86-87	1.0932582046295112E-4	0.0	0.0	5.994968825742295	2.1865164092590225E-5
88	1.0932582046295112E-4	0.0	0.0	6.384474858887697	2.1865164092590225E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14760	0.0	60.573135	1
GTATCAA	27300	0.0	42.005455	1
TGGTATC	5180	0.0	35.409092	2
GTGGTAT	5260	0.0	35.247093	1
ATAACGC	425	0.0	34.304626	3
TACCTGG	4325	0.0	33.166042	2
ATCAACG	36105	0.0	31.601185	3
TATCAAC	36225	0.0	31.561415	2
TCAACGC	36570	0.0	31.237946	4
CAACGCA	38085	0.0	29.968542	5
TATAACG	465	0.0	29.330872	2
AACGCAG	39540	0.0	28.958649	6
GTACCTG	5195	0.0	27.898317	1
GCGGTAT	320	0.0	26.468866	1
CGCAGAG	43790	0.0	25.79387	8
ACGCAGA	44120	0.0	25.728785	7
ACCTGGG	5500	0.0	24.968939	3
GCAGAGT	45895	0.0	24.293325	9
GTACATG	20180	0.0	23.854334	1
TCTGTCG	1245	0.0	23.785313	8
>>END_MODULE