FastQCFastQC Report
Wed 25 May 2016
SRR2431456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170196
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210600.22965703241239338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97000.041.3413051
GTATCAA162350.031.3894631
TCAACGC164050.029.6572384
ATCAACG167350.029.1289183
CAACGCA179050.027.2774055
TATCAAC192950.025.653992
AACGCAG195450.025.009916
GTACATG279950.023.8459571
TACATGG283450.022.5876982
CGCAGAG209500.022.3221998
ACGCAGA215550.022.154177
ACATGGG287000.021.456593
CATGGGT103100.019.6510244
GTGGTAT64600.019.6488931
TGGTATC58200.019.2231852
TCTGTCG21500.019.0186068
GCAGAGT239350.018.3994189
AGAGTAC208450.016.22274810-11
CTGTCGC24700.016.1740919
CAGAGTA234650.016.08386810-11
Produced by FastQC (version 0.11.5)