FastQCFastQC Report
Wed 25 May 2016
SRR2431456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170196
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364230.39718889323630596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324070.35339484564997303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222980.24315728911355874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178950.056.7923361
GTATCAA331750.040.1084751
ATCAACG416950.031.548383
TCAACGC418050.031.5326964
TATCAAC430500.030.620942
CAACGCA436000.030.2218065
GTGGTAT74800.029.0608331
AACGCAG456300.028.9059896
TGGTATC76000.027.6606562
ACGCAGA513450.025.551267
CGCAGAG511700.025.519228
GCAGAGT554250.023.1445359
GTACATG275350.022.1456071
TACATGG276950.021.3622912
GAGTACT391950.021.18662612-13
ACATGGG278550.020.6993123
AGAGTAC526750.020.64949410-11
CAGAGTA550900.020.18796710-11
AGTACTT417150.019.51807212-13
CATGGGT104850.019.0179464
Produced by FastQC (version 0.11.5)