##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431456_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9170196
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25976762110646	33.0	31.0	34.0	30.0	34.0
2	32.40496855247151	34.0	31.0	34.0	31.0	34.0
3	32.52424615569831	34.0	31.0	34.0	31.0	34.0
4	36.06934126598821	37.0	35.0	37.0	35.0	37.0
5	35.96150104098102	37.0	35.0	37.0	35.0	37.0
6	35.99836677427614	37.0	35.0	37.0	35.0	37.0
7	35.94189698889751	37.0	35.0	37.0	35.0	37.0
8	35.9244980150915	37.0	35.0	37.0	35.0	37.0
9	37.62032403669453	39.0	37.0	39.0	35.0	39.0
10-11	37.55839308123839	39.0	37.0	39.0	34.5	39.0
12-13	37.54182331544495	39.0	37.0	39.0	35.0	39.0
14-15	38.91518834493832	40.0	38.0	41.0	35.0	41.0
16-17	38.964844153821794	40.0	38.0	41.0	35.0	41.0
18-19	39.03008250859632	40.5	38.5	41.0	35.0	41.0
20-21	39.05423815368832	41.0	39.0	41.0	35.0	41.0
22-23	39.00867484184634	41.0	39.0	41.0	35.0	41.0
24-25	38.968253132212226	41.0	38.5	41.0	35.0	41.0
26-27	38.840975263778446	40.0	38.0	41.0	35.0	41.0
28-29	38.758720478820734	40.0	38.0	41.0	34.5	41.0
30-31	38.616400510959636	40.0	38.0	41.0	34.0	41.0
32-33	38.45572373807495	40.0	38.0	41.0	34.0	41.0
34-35	38.32883522882172	40.0	38.0	41.0	34.0	41.0
36-37	38.19858146979628	40.0	38.0	41.0	34.0	41.0
38-39	38.09914875320004	40.0	38.0	41.0	33.5	41.0
40-41	37.995914427565125	40.0	38.0	41.0	33.0	41.0
42-43	37.90146442889552	40.0	38.0	41.0	33.0	41.0
44-45	37.732624962432645	40.0	38.0	41.0	33.0	41.0
46-47	37.48629336821154	40.0	37.0	41.0	32.5	41.0
48-49	37.30114132784075	40.0	37.0	41.0	32.0	41.0
50-51	37.20626609289485	40.0	37.0	41.0	31.5	41.0
52-53	37.09510707295678	40.0	37.0	41.0	31.0	41.0
54-55	36.96235729312656	40.0	37.0	41.0	31.0	41.0
56-57	36.76393699763887	40.0	36.0	41.0	31.0	41.0
58-59	36.622373611207436	40.0	36.0	41.0	31.0	41.0
60-61	36.440780382447656	39.0	35.0	41.0	30.5	41.0
62-63	36.23782665059721	39.0	35.0	41.0	30.0	41.0
64-65	36.0168961492208	39.0	35.0	41.0	30.0	41.0
66-67	35.76846830754762	38.5	35.0	41.0	29.5	41.0
68-69	35.49203217684769	38.0	35.0	40.0	29.0	41.0
70-71	35.17026626257498	37.0	35.0	40.0	29.0	41.0
72-73	34.84928888106644	37.0	35.0	39.5	29.0	41.0
74-75	34.531630676160034	36.0	34.0	39.0	29.0	41.0
76-77	34.161153098581536	36.0	34.0	39.0	28.0	40.5
78-79	33.829784717796656	35.0	34.0	37.5	28.0	39.5
80-81	33.50137957792832	35.0	34.0	37.0	28.0	39.0
82-83	33.219274211805285	35.0	34.0	37.0	28.0	39.0
84-85	32.96396440163329	35.0	34.0	36.0	28.0	38.0
86-87	32.69786976199855	35.0	34.0	36.0	27.0	37.0
88-89	32.5249754203727	35.0	34.0	36.0	27.0	37.0
90-91	32.321825345935906	35.0	34.0	35.0	27.0	36.5
92-93	32.11613225060839	35.0	34.0	35.0	27.0	36.0
94-95	31.950933000777738	35.0	34.0	35.0	26.0	36.0
96-97	31.782719366085523	35.0	33.0	35.0	26.0	36.0
98-99	31.61328160270511	35.0	33.0	35.0	25.5	36.0
100	31.506214480039468	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.170424586852306
1101	2	0.244892676622662
1101	3	0.21559271688288106
1101	4	0.16799234297025123
1101	5	0.1618208870202693
1101	6	0.1812633387684599
1101	7	0.19410867430372747
1101	8	0.2530501285721911
1101	9	0.3232312411331719
1101	10-11	0.2786970389761052
1101	12-13	0.33418008699297275
1101	14-15	0.3985994778328532
1101	16-17	0.3858375778909462
1101	18-19	0.38086133994024607
1101	20-21	0.3520405812438838
1101	22-23	0.3615080918164395
1101	24-25	0.34526394628601764
1101	26-27	0.3462823256815284
1101	28-29	0.36543359702843503
1101	30-31	0.3971093273945172
1101	32-33	0.40024728315038516
1101	34-35	0.4181483203388652
1101	36-37	0.4556706810907869
1101	38-39	0.4451201664410078
1101	40-41	0.5166075527845422
1101	42-43	0.5445724758448165
1101	44-45	0.5658301525452885
1101	46-47	0.506007945262752
1101	48-49	0.4791699942648364
1101	50-51	0.4197026639942081
1101	52-53	0.4874927564838316
1101	54-55	0.43613918162040477
1101	56-57	0.4526854371258935
1101	58-59	0.44125546057873066
1101	60-61	0.5029103319102504
1101	62-63	0.5086355095727555
1101	64-65	0.4612529906297951
1101	66-67	0.4564101327996184
1101	68-69	0.385578880362516
1101	70-71	0.43188708151029687
1101	72-73	0.44428683019409476
1101	74-75	0.35644957694722734
1101	76-77	0.3878938114902226
1101	78-79	0.37802861905166196
1101	80-81	0.388527861900279
1101	82-83	0.37233478983907276
1101	84-85	0.3711767271588471
1101	86-87	0.3996593198596301
1101	88-89	0.41648754734339377
1101	90-91	0.4332049176089967
1101	92-93	0.4598221288330251
1101	94-95	0.4900141342398818
1101	96-97	0.5293555934722605
1101	98-99	0.5682097256698739
1101	100	0.6049516542875573
1102	1	0.1284549385820597
1102	2	0.21791311991188422
1102	3	0.20379803895119863
1102	4	0.12414332939730599
1102	5	0.15977011067776203
1102	6	0.10408097595169608
1102	7	0.13095341458861753
1102	8	0.1508548189010881
1102	9	0.23287753309706005
1102	10-11	0.2511198506468517
1102	12-13	0.26879846033249066
1102	14-15	0.3378310270447429
1102	16-17	0.33714343799569235
1102	18-19	0.3473527489004411
1102	20-21	0.29129148311770336
1102	22-23	0.30040554829960087
1102	24-25	0.264535392517395
1102	26-27	0.281899945035228
1102	28-29	0.30342763147630336
1102	30-31	0.28360255969673887
1102	32-33	0.2888828872556175
1102	34-35	0.3904772540059014
1102	36-37	0.3171261278177795
1102	38-39	0.30259457019727876
1102	40-41	0.369218022184846
1102	42-43	0.3558417092698676
1102	44-45	0.33489014103638226
1102	46-47	0.3664728477890975
1102	48-49	0.32210825718654945
1102	50-51	0.3231551648085187
1102	52-53	0.3223563851136362
1102	54-55	0.3220733927770212
1102	56-57	0.3843817512751144
1102	58-59	0.36247162750551354
1102	60-61	0.2987066822301401
1102	62-63	0.32390027675224786
1102	64-65	0.36056781277023475
1102	66-67	0.3597223092215813
1102	68-69	0.32358601036660417
1102	70-71	0.332836709441068
1102	72-73	0.32276159223008705
1102	74-75	0.37134847998756015
1102	76-77	0.36375494711776213
1102	78-79	0.3829493048975152
1102	80-81	0.3382000636057185
1102	82-83	0.32444659269744847
1102	84-85	0.3235298492973371
1102	86-87	0.33779065264725716
1102	88-89	0.33451922074521434
1102	90-91	0.32717766766576517
1102	92-93	0.34555814119406136
1102	94-95	0.3864270606749436
1102	96-97	0.36139543159846
1102	98-99	0.4143084603380629
1102	100	0.39805603862347994
1103	1	0.10709884483880217
1103	2	0.18619455616703107
1103	3	0.15307210831656448
1103	4	0.16454189170487155
1103	5	0.12161478033302586
1103	6	0.15659983319307003
1103	7	0.15672064249759643
1103	8	0.17421209969326412
1103	9	0.27164812768145197
1103	10-11	0.2459041132394617
1103	12-13	0.22357605218294196
1103	14-15	0.32205369654631255
1103	16-17	0.30113244853716026
1103	18-19	0.28099539467716994
1103	20-21	0.2787372113958142
1103	22-23	0.24638244546441257
1103	24-25	0.3157068462312935
1103	26-27	0.30821270850248794
1103	28-29	0.3057061668474219
1103	30-31	0.3198231920670551
1103	32-33	0.3600026771904865
1103	34-35	0.3556795981901857
1103	36-37	0.37847300873614387
1103	38-39	0.34025375415525616
1103	40-41	0.3523966186029739
1103	42-43	0.3856408296726954
1103	44-45	0.37039928216272244
1103	46-47	0.3370992372897206
1103	48-49	0.3058577601501611
1103	50-51	0.3283376636119968
1103	52-53	0.34989114490876716
1103	54-55	0.33986005680833387
1103	56-57	0.3405116831968513
1103	58-59	0.3576711946465636
1103	60-61	0.37818674051553813
1103	62-63	0.3703213335134379
1103	64-65	0.33032690136273146
1103	66-67	0.33826427466188846
1103	68-69	0.2873803893614948
1103	70-71	0.3274073241133948
1103	72-73	0.33067018462161
1103	74-75	0.3049838930156028
1103	76-77	0.3349820463604729
1103	78-79	0.3510002069298608
1103	80-81	0.32565024782661567
1103	82-83	0.29225803675470274
1103	84-85	0.3328544702953664
1103	86-87	0.3040352127777055
1103	88-89	0.34525950841565844
1103	90-91	0.36960924942317064
1103	92-93	0.35355616690879543
1103	94-95	0.3937087832021682
1103	96-97	0.38890642834314093
1103	98-99	0.43698166194447197
1103	100	0.47082793313276383
1104	1	0.09289456187612188
1104	2	0.1612854416787428
1104	3	0.12531504349789913
1104	4	0.1319234533222442
1104	5	0.09909607695156808
1104	6	0.12287020850433095
1104	7	0.11509161195575501
1104	8	0.13906585624462764
1104	9	0.20841447647244138
1104	10-11	0.1967998332674199
1104	12-13	0.22462000935695414
1104	14-15	0.3135643619262183
1104	16-17	0.2701134059713297
1104	18-19	0.24560186613694412
1104	20-21	0.26450642133917057
1104	22-23	0.23961155681855217
1104	24-25	0.23200210456197823
1104	26-27	0.23526514300223056
1104	28-29	0.2637225266687224
1104	30-31	0.24710577818703428
1104	32-33	0.226319410730909
1104	34-35	0.2703946800639727
1104	36-37	0.2717726076721405
1104	38-39	0.23450193451364498
1104	40-41	0.2770617837045748
1104	42-43	0.2332299665574311
1104	44-45	0.2979398317502273
1104	46-47	0.31821520757691246
1104	48-49	0.24618173349212924
1104	50-51	0.2218965221849416
1104	52-53	0.19474920989297573
1104	54-55	0.23285292712110817
1104	56-57	0.24870967669911437
1104	58-59	0.2784896464956219
1104	60-61	0.2620271782479122
1104	62-63	0.27061490816296185
1104	64-65	0.2607927250832063
1104	66-67	0.29007213744193905
1104	68-69	0.3138061851726164
1104	70-71	0.3154435687187913
1104	72-73	0.2939595762662961
1104	74-75	0.31202257369847786
1104	76-77	0.3170913717106387
1104	78-79	0.2984336352389292
1104	80-81	0.32826549711742103
1104	82-83	0.32858275572885987
1104	84-85	0.3314207695675293
1104	86-87	0.32412618223999345
1104	88-89	0.2736334777764142
1104	90-91	0.3230452726806021
1104	92-93	0.35616269495366737
1104	94-95	0.3278734109342416
1104	96-97	0.3369553311159805
1104	98-99	0.3758395904102727
1104	100	0.4548916931317706
1105	1	0.0554044780084908
1105	2	0.11448448556307511
1105	3	0.09490213739891118
1105	4	0.07587446316256319
1105	5	0.06676041196026006
1105	6	0.08260569439006105
1105	7	0.12827341106028456
1105	8	0.12884803705952663
1105	9	0.15189777241170077
1105	10-11	0.11198671365209378
1105	12-13	0.07842595109312356
1105	14-15	0.16846462998358191
1105	16-17	0.1763676036017614
1105	18-19	0.18659604131590157
1105	20-21	0.1864153618366302
1105	22-23	0.192912729478941
1105	24-25	0.20065079903485383
1105	26-27	0.1851256401859871
1105	28-29	0.180076062417875
1105	30-31	0.17934269671370373
1105	32-33	0.13731139859277874
1105	34-35	0.15367452746797028
1105	36-37	0.1421306732477774
1105	38-39	0.12576204184009754
1105	40-41	0.13764246513715506
1105	42-43	0.1418466903410831
1105	44-45	0.17036807724914382
1105	46-47	0.21009310426536132
1105	48-49	0.1748639278729982
1105	50-51	0.20298667186909114
1105	52-53	0.21759232443349674
1105	54-55	0.2501042344946498
1105	56-57	0.2974710295208354
1105	58-59	0.25674642590144003
1105	60-61	0.2174364190632474
1105	62-63	0.24447278791180338
1105	64-65	0.23547922939024346
1105	66-67	0.255389764362846
1105	68-69	0.2724280208207901
1105	70-71	0.2630575333310077
1105	72-73	0.27959845899768254
1105	74-75	0.2353603905142947
1105	76-77	0.2686482750265853
1105	78-79	0.186192941745567
1105	80-81	0.14690024164256243
1105	82-83	0.15916560711620775
1105	84-85	0.1531888850421126
1105	86-87	0.16686755832253652
1105	88-89	0.15390820994359444
1105	90-91	0.14515397322427503
1105	92-93	0.19980712814822255
1105	94-95	0.19400580762441066
1105	96-97	0.19755270707671713
1105	98-99	0.20094012569360942
1105	100	0.21705401583212236
1106	1	0.09939856646374068
1106	2	0.168218739273577
1106	3	0.14114176978946347
1106	4	0.13203154398510009
1106	5	0.10875428790773611
1106	6	0.12014829947562333
1106	7	0.10138073589775587
1106	8	0.15748096204212203
1106	9	0.20043580174788644
1106	10-11	0.15156656863696583
1106	12-13	0.19172189768940484
1106	14-15	0.23391658139245664
1106	16-17	0.19145907169821896
1106	18-19	0.220095294428738
1106	20-21	0.20700470319562925
1106	22-23	0.1910746774191736
1106	24-25	0.2308538290399227
1106	26-27	0.2230441220232251
1106	28-29	0.2099616843139458
1106	30-31	0.18046736204377112
1106	32-33	0.19859800909152625
1106	34-35	0.24360246625567328
1106	36-37	0.22876200219837273
1106	38-39	0.217393354193554
1106	40-41	0.2341405326345054
1106	42-43	0.2920530603985867
1106	44-45	0.27362845376947575
1106	46-47	0.21648473321221928
1106	48-49	0.26164383340339725
1106	50-51	0.24170539340116903
1106	52-53	0.24239491280010128
1106	54-55	0.2493073303403932
1106	56-57	0.26883651733655967
1106	58-59	0.2564104386702155
1106	60-61	0.24110365064479566
1106	62-63	0.19515465297723011
1106	64-65	0.24165155862853283
1106	66-67	0.22620891641170715
1106	68-69	0.2683325167846746
1106	70-71	0.22477654107503753
1106	72-73	0.24035577560265864
1106	74-75	0.23044168472853954
1106	76-77	0.22291334054353484
1106	78-79	0.21964329776698577
1106	80-81	0.21685974914660733
1106	82-83	0.20748003437987705
1106	84-85	0.226696126694101
1106	86-87	0.20414903247549887
1106	88-89	0.1933788138888204
1106	90-91	0.21836588256730494
1106	92-93	0.21281487436920798
1106	94-95	0.20337568758973745
1106	96-97	0.19453878833312643
1106	98-99	0.2643532561152142
1106	100	0.2851519452413207
1107	1	0.10262013299158212
1107	2	0.12128842180590738
1107	3	0.12667990695264564
1107	4	0.10697185135344256
1107	5	0.10122490050028432
1107	6	0.12066358660968746
1107	7	0.0419525885533929
1107	8	0.06968277190084393
1107	9	0.11637676984732082
1107	10-11	0.08441453160595103
1107	12-13	0.031603496147553756
1107	14-15	0.14951533518260618
1107	16-17	0.12977499980446794
1107	18-19	0.09942445156288926
1107	20-21	0.14225785650986467
1107	22-23	0.13805180964168073
1107	24-25	0.11647741654363841
1107	26-27	0.14044728801498962
1107	28-29	0.15382467883555506
1107	30-31	0.16943397207045052
1107	32-33	0.16787940758418785
1107	34-35	0.1518269406979158
1107	36-37	0.1903480523263923
1107	38-39	0.15435130142325448
1107	40-41	0.12726384521813117
1107	42-43	0.11599739633230399
1107	44-45	0.14655568913934758
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2216	76-77	-0.7828047717912838
2216	78-79	-0.7150632801290513
2216	80-81	-0.7360429190571764
2216	82-83	-0.8000249530940948
2216	84-85	-0.8161618988467865
2216	86-87	-0.858880927427041
2216	88-89	-0.834466812431863
2216	90-91	-0.8549212354342082
2216	92-93	-0.8228769402060401
2216	94-95	-0.8928740129504718
2216	96-97	-1.0736333389757249
2216	98-99	-1.1613987438161608
2216	100	-1.1652176692533907
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	246.0
3	728.0
4	503.0
5	459.0
6	706.0
7	1635.0
8	3276.0
9	5750.0
10	8198.0
11	11033.0
12	15145.0
13	21345.0
14	25664.0
15	33545.0
16	41277.0
17	56410.0
18	62692.0
19	36731.0
20	25423.0
21	23970.0
22	24595.0
23	26664.0
24	31079.0
25	38482.0
26	49292.0
27	64763.0
28	82826.0
29	106419.0
30	134799.0
31	170642.0
32	213482.0
33	272047.0
34	361083.0
35	522652.0
36	818707.0
37	1586808.0
38	2725700.0
39	1554867.0
40	10553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.060015776398465	22.107873033814517	14.942191391934397	24.88991979785262
2	13.298221761017976	21.966586101322154	40.02798849664718	24.70720364101269
3	15.504348008151641	26.97244851134809	32.19612142667709	25.327082053823176
4	10.961086177078869	18.85513724969816	39.51720128122416	30.66657529199881
5	11.40727563763168	38.6021302863883	36.11228132317195	13.878312752808064
6	28.209247458750873	40.24293921775927	18.08808863933646	13.45972468415339
7	24.468844504523133	34.24667259020418	23.53396808530592	17.75051481996677
8	24.00486314578227	36.392853544242676	21.15081291610343	18.451470393871624
9	24.14901491745651	16.665118171956195	22.763602871737966	36.422264038849335
10-11	22.35597256715885	27.794188472744928	29.90609495574075	19.94374400435547
12-13	23.57814380412371	25.601006783279225	29.984708069489464	20.836141343107606
14-15	20.922464470770308	27.602245360949755	27.94931536904991	23.525974799230028
16-17	19.372704792787417	30.798943664890043	28.74409663653863	21.08425490578391
18-19	19.208444399661687	28.845457610720644	32.09169684050374	19.854401149113933
20-21	20.5166988797186	28.139545763253043	31.774560761841954	19.569194595186406
22-23	20.119493629143808	28.15883651777999	31.775275032289386	19.94639482078682
24-25	20.00083641021243	28.13180690832306	31.8095527751671	20.057803906297412
26-27	19.787406943101324	28.005213847119514	31.935189825822697	20.27218938395646
28-29	19.81869361608206	28.04324849882794	32.015989238147	20.122068646943
30-31	20.215391581351813	28.44079247909755	31.59529641520037	19.74851952435027
32-33	19.751710651951736	28.21811831992975	31.973363032469997	20.056807995648505
34-35	19.94831757304479	27.93461185535529	31.81216791657922	20.304902655020697
36-37	20.30834651108226	28.301218994257628	31.245510064081454	20.14492443057866
38-39	20.1778918009173	28.304085187586114	31.412649752834454	20.105373258662134
40-41	20.060246863067203	28.118955902790198	31.53854634582116	20.28225088832144
42-43	20.027644447691742	28.143263669688658	31.378590983766113	20.45050089885349
44-45	20.306659748602975	28.309198625634615	31.04223726515769	20.341904360604723
46-47	20.35880609306575	28.589374975054966	30.570463204466837	20.48135572741245
48-49	20.327306264914206	28.430705797583084	30.474945495756206	20.767042441746504
50-51	20.368925551916963	28.385971021023142	30.293918966628524	20.95118446043137
52-53	20.38379530916844	28.579694546065227	30.143463187105734	20.893046957660598
54-55	20.58088034714131	28.903713520634483	29.904351627193538	20.611054505030673
56-57	20.375799221135466	28.55101413301223	30.009437130536504	21.063749515315802
58-59	20.237351042143345	28.212068394651237	30.407393221695006	21.143187341510412
60-61	20.477799900415416	28.613158163918495	29.93986212136975	20.969179814296343
62-63	20.329284395073664	28.500505344834053	30.126567397777265	21.043642862315014
64-65	20.279999081798604	28.33054792250664	30.28149688419479	21.107956111499966
66-67	20.240421422043678	28.40914574338949	30.052056176156444	21.298376658410383
68-69	20.6487303121614	28.481685315624823	29.87635505196874	20.99322932024504
70-71	20.61779998917141	28.280903453409827	29.872231900646103	21.229064656772664
72-73	20.385954673160747	28.39567987423606	30.085147580269822	21.133217872333372
74-75	20.437082218941537	28.374630623148484	29.901613644408492	21.28667351350149
76-77	20.487468315835343	28.390538217503746	29.687958687033518	21.434034779627392
78-79	20.587254810230856	28.417766049860514	29.773808300896544	21.22117083901209
80-81	20.388871731858295	28.28308140851079	29.872769349749994	21.455277509880926
82-83	20.231914819602117	28.300509776838638	30.027172031335347	21.440403372223894
84-85	20.313503341251604	28.20013736974506	29.83927238200196	21.64708690700138
86-87	20.399711194831603	28.319787276084398	29.70173156604286	21.57876996304114
88-89	20.2014547998756	28.17086461401697	29.819924241532025	21.807756344575406
90-91	20.090438638958133	28.137137316210982	29.72977511766243	22.042648927168457
92-93	20.179849544691944	28.226313266991383	29.65784148922075	21.93599569909593
94-95	20.006622541110353	28.01900853591352	29.853145996007065	22.121222926969065
96-97	20.012692267155266	28.063056326065748	29.735498888486074	22.18875251829291
98-99	19.95977050727045	28.00976931862966	29.736733514806428	22.29372665929346
100	20.042766993276885	28.036764041963952	29.584589400660143	22.335879564099017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4708.0
1	2791.0
2	2325.0
3	3973.0
4	2722.5
5	1559.0
6	3825.0
7	10050.0
8	16442.0
9	17951.5
10	12742.0
11	7176.0
12	6312.5
13	6781.0
14	7616.0
15	8460.5
16	9247.0
17	10402.5
18	11924.0
19	13866.5
20	16584.5
21	20693.0
22	27374.0
23	37528.5
24	50285.0
25	66954.5
26	90378.5
27	118813.0
28	152099.0
29	184973.0
30	212983.5
31	238961.5
32	261996.5
33	283283.0
34	301954.0
35	315467.5
36	324738.5
37	332254.0
38	336702.5
39	335868.0
40	333401.5
41	328205.0
42	321004.5
43	314769.0
44	307127.5
45	297825.0
46	288882.0
47	281027.0
48	270893.5
49	260820.0
50	254164.5
51	240721.0
52	221473.0
53	205842.5
54	194368.0
55	183660.5
56	166059.5
57	145527.0
58	130396.0
59	117273.0
60	98954.0
61	79311.5
62	64232.0
63	50819.0
64	38757.0
65	29114.5
66	21568.0
67	15892.0
68	11720.0
69	8485.0
70	5938.0
71	4231.5
72	3104.0
73	2178.5
74	1445.0
75	975.0
76	633.5
77	400.0
78	249.0
79	144.5
80	83.5
81	47.5
82	20.5
83	7.0
84	4.5
85	3.0
86	2.5
87	3.0
88	2.0
89	1.0
90	2.0
91	2.0
92	0.5
93	1.5
94	4.0
95	3.5
96	1.0
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05022793405942468
2	0.0
3	2.180978465454828E-4
4	0.07044560443419094
5	0.004830867300982444
6	1.090489232727414E-5
7	0.0
8	0.0
9	0.0
10-11	0.011341088020365105
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.943166318364406E-4
26-27	0.0
28-29	3.053369851636759E-4
30-31	0.0036967584989459333
32-33	8.723913861819312E-5
34-35	0.0016848058645638545
36-37	0.0041711213151823575
38-39	0.0013903737717274526
40-41	0.0010032500941092209
42-43	0.001973785511236619
44-45	0.0
46-47	3.707663391273207E-4
48-49	4.1983835460005436E-4
50-51	0.0014667080180183717
52-53	8.015095860546492E-4
54-55	0.0010632270019092286
56-57	0.006166716611073525
58-59	0.00898563127767389
60-61	0.0011122990173819622
62-63	0.0023990763120003106
64-65	0.001030512324927406
66-67	7.08818001272819E-5
68-69	7.415326782546414E-4
70-71	2.998845390000388E-4
72-73	0.0
74-75	2.3990763120003105E-4
76-77	0.0
78-79	5.888641856728036E-4
80-81	0.0
82-83	0.0011450136943637844
84-85	5.943166318364406E-4
86-87	0.0
88-89	0.0
90-91	5.45244616363707E-6
92-93	3.380516621454983E-4
94-95	0.0
96-97	4.961726008909734E-4
98-99	6.324837549819001E-4
100	4.361956930909656E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9170196.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.29156872427531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.38057807534199	37.12488853983103
2	18.264268832389334	19.8319161302461
3	6.472952857375996	10.542802947156694
4	2.7507765982776995	5.973759069220879
5	1.3705441518792116	3.720449600570192
6	0.7514626594472188	2.4478851971463222
7	0.45951392400158175	1.7463412249285308
8	0.3126703251369121	1.3580289956169733
9	0.2195803069947113	1.0729223394930754
>10	0.9312487757181135	8.638833659236846
>50	0.05359961407804984	2.0065463680751026
>100	0.030368850302253583	3.0568093783536776
>500	0.001570090926980518	0.5925138114261195
>1k	7.241343930352364E-4	0.5962134657940866
>5k	8.045927813138809E-5	0.2949121821572213
>10k+	6.034445859854108E-5	0.9951770907470194
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36423	0.39718889323630596	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32407	0.35339484564997303	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22298	0.24315728911355874	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.0904892327274138E-5	0.0	1.0904892327274138E-5	0.0
4	0.0	1.0904892327274138E-5	0.0	2.1809784654548276E-5	0.0
5	0.0	1.0904892327274138E-5	0.0	3.271467698182242E-5	0.0
6	0.0	1.0904892327274138E-5	0.0	3.271467698182242E-5	0.0
7	0.0	1.0904892327274138E-5	0.0	4.361956930909655E-5	0.0
8	0.0	1.0904892327274138E-5	0.0	6.542935396364484E-5	0.0
9	0.0	1.0904892327274138E-5	0.0	1.0904892327274139E-4	0.0
10-11	0.0	2.180978465454828E-5	0.0	1.254062617636526E-4	0.0
12-13	0.0	4.361956930909656E-5	0.0	1.4176360025456382E-4	0.0
14-15	0.0	5.452446163637069E-5	0.0	2.0174050805457156E-4	0.0
16-17	0.0	5.452446163637069E-5	0.0	2.780747543454906E-4	0.0
18-19	0.0	6.542935396364484E-5	0.0	2.889796466727647E-4	0.0
20-21	0.0	8.178669245455605E-5	0.0	3.1624187749095E-4	0.0
22-23	0.0	9.814403094546725E-5	0.0	4.907201547273362E-4	0.0
24-25	0.0	9.814403094546725E-5	0.0	8.832962785092053E-4	0.0
26-27	0.0	9.814403094546725E-5	0.0	0.0014721604641820087	0.0
28-29	0.0	9.814403094546725E-5	0.0	0.003756735406745941	0.0
30-31	0.0	9.814403094546725E-5	0.0	0.009650829709637612	0.0
32-33	0.0	1.0359647710910432E-4	0.0	0.023216515764766642	0.0
34-35	0.0	1.0904892327274139E-4	0.0	0.046967371253569715	0.0
36-37	0.0	1.0904892327274139E-4	0.0	0.08376047796579267	0.0
38-39	0.0	1.0904892327274139E-4	0.0	0.1302316766184714	0.0
40-41	0.0	1.0904892327274139E-4	0.0	0.18179545998798718	0.0
42-43	0.0	1.0904892327274139E-4	0.0	0.2356928903155396	0.0
44-45	0.0	1.0904892327274139E-4	0.0	0.30366853663760296	0.0
46-47	0.0	1.0904892327274139E-4	0.0	0.388715791898014	0.0
48-49	0.0	1.0904892327274139E-4	0.0	0.47767790350391637	0.0
50-51	0.0	1.3085870792728968E-4	0.0	0.5684011552206736	0.0
52-53	5.452446163637069E-6	1.3631115409092675E-4	0.0	0.6590099055679943	0.0
54-55	1.0904892327274138E-5	1.6902583107274914E-4	0.0	0.7656052280670991	0.0
56-57	1.0904892327274138E-5	1.962880618909345E-4	0.0	0.8963766968557706	0.0
58-59	1.0904892327274138E-5	1.962880618909345E-4	0.0	1.0399177945596803	0.0
60-61	1.0904892327274138E-5	1.962880618909345E-4	0.0	1.1915612272627543	0.0
62-63	1.0904892327274138E-5	1.962880618909345E-4	0.0	1.343106515934883	1.0904892327274138E-5
64-65	1.0904892327274138E-5	1.962880618909345E-4	0.0	1.5118542722532866	1.0904892327274138E-5
66-67	1.0904892327274138E-5	1.962880618909345E-4	0.0	1.709707186193185	1.0904892327274138E-5
68-69	1.0904892327274138E-5	1.962880618909345E-4	0.0	1.934620590443214	1.0904892327274138E-5
70-71	1.0904892327274138E-5	1.962880618909345E-4	0.0	2.174244694442736	1.0904892327274138E-5
72-73	1.0904892327274138E-5	2.0719295421820863E-4	0.0	2.4280615158062053	1.0904892327274138E-5
74-75	1.0904892327274138E-5	2.0719295421820863E-4	0.0	2.6885630361662933	1.0904892327274138E-5
76-77	1.0904892327274138E-5	2.1809784654548277E-4	0.0	2.9756016120048034	1.0904892327274138E-5
78-79	1.0904892327274138E-5	2.1809784654548277E-4	0.0	3.302650237792082	1.0904892327274138E-5
80-81	1.0904892327274138E-5	2.1809784654548277E-4	0.0	3.660128965618619	1.0904892327274138E-5
82-83	1.0904892327274138E-5	2.1809784654548277E-4	0.0	4.029025115711812	1.0904892327274138E-5
84-85	1.0904892327274138E-5	2.1809784654548277E-4	0.0	4.403177423906752	1.0904892327274138E-5
86-87	1.0904892327274138E-5	2.1809784654548277E-4	0.0	4.805693357044931	1.0904892327274138E-5
88	1.0904892327274138E-5	2.1809784654548277E-4	0.0	5.133576207095246	1.0904892327274138E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17895	0.0	56.792336	1
GTATCAA	33175	0.0	40.108475	1
ATCAACG	41695	0.0	31.54838	3
TCAACGC	41805	0.0	31.532696	4
TATCAAC	43050	0.0	30.62094	2
CAACGCA	43600	0.0	30.221806	5
GTGGTAT	7480	0.0	29.060833	1
AACGCAG	45630	0.0	28.905989	6
TGGTATC	7600	0.0	27.660656	2
ACGCAGA	51345	0.0	25.55126	7
CGCAGAG	51170	0.0	25.51922	8
GCAGAGT	55425	0.0	23.144535	9
GTACATG	27535	0.0	22.145607	1
TACATGG	27695	0.0	21.362291	2
GAGTACT	39195	0.0	21.186626	12-13
ACATGGG	27855	0.0	20.699312	3
AGAGTAC	52675	0.0	20.649494	10-11
CAGAGTA	55090	0.0	20.187967	10-11
AGTACTT	41715	0.0	19.518072	12-13
CATGGGT	10485	0.0	19.017946	4
>>END_MODULE