FastQCFastQC Report
Wed 25 May 2016
SRR2431455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999699
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA306391.5321805931792734No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29400.14702212683008792No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22060.11031660264869862No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG24600.049.111192
GTACCTG26950.045.178761
TCTGTCG7400.037.4727758
GGTATCA35900.035.3559571
GTCTCGC7850.032.3383141
CGCTCTG7850.031.7386655
TCGCTCT9050.031.166294
ACCTGGG39050.029.975113
TATAACG1602.1318556E-929.3807242
GTATCAA52400.028.4394551
TCTCGCT9300.028.3065872
CTGTCGC10700.027.672759
GTACCCG1755.5460987E-926.8630451
GTACACG2650.026.609621
CTCGCTC9900.026.1161983
GTACATG81700.025.375221
TACATGG81400.024.8328462
ATCAACG57300.024.4480423
TCAACGC57400.024.4054494
GGCTAGA7100.023.8362241
Produced by FastQC (version 0.11.5)