##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431455_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1999699
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.987328092877977	31.0	30.0	34.0	20.0	34.0
2	29.042790439961212	31.0	30.0	34.0	18.0	34.0
3	29.16612000106016	31.0	30.0	34.0	20.0	34.0
4	32.3303472172562	37.0	33.0	37.0	19.0	37.0
5	32.535965662832254	37.0	33.0	37.0	22.0	37.0
6	32.50520503335752	37.0	33.0	37.0	19.0	37.0
7	32.37045575359092	37.0	33.0	37.0	18.0	37.0
8	32.2576462757645	37.0	33.0	37.0	17.0	37.0
9	33.45075083800112	39.0	34.0	39.0	15.0	39.0
10-11	33.39533399776666	39.0	34.0	39.0	14.5	39.0
12-13	33.3359695634193	39.0	34.0	39.0	13.0	39.0
14-15	34.40230304660851	40.0	34.0	41.0	13.0	41.0
16-17	34.38298163873663	40.0	34.0	41.0	13.0	41.0
18-19	34.342456289671595	40.0	34.0	41.0	13.0	41.0
20-21	34.42187299188528	40.0	34.0	41.0	16.5	41.0
22-23	34.38768384641888	39.5	34.0	41.0	17.0	41.0
24-25	34.43965016735018	39.5	34.0	41.0	18.0	41.0
26-27	34.443716029262404	39.5	34.0	41.0	18.0	41.0
28-29	34.35336493142218	39.0	33.0	41.0	18.0	41.0
30-31	34.31804436567703	39.0	33.0	41.0	18.0	41.0
32-33	34.24118404819926	39.0	33.0	41.0	18.0	41.0
34-35	34.13286124561746	39.0	33.0	41.0	16.5	41.0
36-37	33.987400853828504	39.0	33.0	41.0	16.0	41.0
38-39	33.85714450024729	39.0	33.0	41.0	15.0	41.0
40-41	33.7529265654481	39.0	33.0	41.0	12.0	41.0
42-43	33.531619508736064	39.0	32.5	41.0	9.0	41.0
44-45	33.40034800237436	38.0	32.0	41.0	8.5	41.0
46-47	33.25424701417563	38.0	32.0	40.0	7.5	41.0
48-49	33.106440519298154	38.0	32.0	40.0	4.0	41.0
50-51	32.94549779741851	38.0	32.0	40.0	2.0	41.0
52-53	32.83384324340814	38.0	31.0	40.0	2.0	41.0
54-55	32.75213794676099	38.0	31.0	40.0	2.0	41.0
56-57	32.59635450135245	38.0	31.0	40.0	2.0	41.0
58-59	32.378967534613956	37.5	31.0	40.0	2.0	41.0
60-61	32.13267446750736	37.0	31.0	40.0	2.0	41.0
62-63	31.80652038131739	36.5	30.5	40.0	2.0	41.0
64-65	31.623091275236924	36.0	30.0	40.0	2.0	41.0
66-67	31.352941117638203	36.0	30.0	39.0	2.0	41.0
68-69	31.054236412580092	35.0	30.0	39.0	2.0	41.0
70-71	30.72662310677757	35.0	30.0	39.0	2.0	41.0
72-73	30.399211581342993	35.0	30.0	38.0	2.0	40.0
74-75	30.078448306470122	35.0	30.0	37.0	2.0	40.0
76-77	29.76515265547465	35.0	29.0	37.0	2.0	39.0
78-79	29.439233604657502	35.0	29.0	36.0	2.0	39.0
80-81	29.144043178498364	34.5	29.0	36.0	2.0	38.5
82-83	28.862208012305853	34.0	29.0	35.5	2.0	37.0
84-85	28.5792186724102	34.0	29.0	35.0	2.0	37.0
86-87	28.322832086228978	34.0	28.0	35.0	2.0	36.5
88-89	28.057678930679067	34.0	28.0	35.0	2.0	36.0
90-91	27.84420255248415	34.0	27.5	35.0	2.0	36.0
92-93	27.62826055321326	34.0	27.0	35.0	2.0	36.0
94-95	27.383010893139417	34.0	27.0	35.0	2.0	35.0
96-97	27.271182813013358	34.0	27.0	35.0	2.0	35.0
98-99	27.08992428360468	34.0	27.0	35.0	2.0	35.0
100	26.935364772398245	34.0	26.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4166518516308919
1101	2	0.4465001732358296
1101	3	0.5312483360367075
1101	4	0.4197393321664862
1101	5	0.31316739636782387
1101	6	0.389459803843323
1101	7	0.3769538795496885
1101	8	0.38420443089041356
1101	9	0.5305255420221187
1101	10-11	0.5766362109780729
1101	12-13	0.49193469992307826
1101	14-15	0.7076951066899326
1101	16-17	0.6987289996544561
1101	18-19	0.5787014086973059
1101	20-21	0.48668952836946033
1101	22-23	0.6051361746648212
1101	24-25	0.5726095792748893
1101	26-27	0.7261812185533287
1101	28-29	0.7169057276032902
1101	30-31	0.7109187167634303
1101	32-33	0.7556519889068412
1101	34-35	0.6778482009140134
1101	36-37	0.750773627704028
1101	38-39	0.6978339109169553
1101	40-41	0.6936459453601458
1101	42-43	0.7064416441524415
1101	44-45	0.7480680477026098
1101	46-47	0.7260450133110723
1101	48-49	0.7010966811054047
1101	50-51	0.7634055191997788
1101	52-53	0.7530779316101004
1101	54-55	0.7254869464224925
1101	56-57	0.6126112261049457
1101	58-59	0.6835354828419753
1101	60-61	0.6999268310372884
1101	62-63	0.7426571493729561
1101	64-65	0.6958034558323263
1101	66-67	0.7228547526614761
1101	68-69	0.6472676413056639
1101	70-71	0.7580477770052312
1101	72-73	0.6510602901410856
1101	74-75	0.5400877686601362
1101	76-77	0.6525186052337766
1101	78-79	0.6366684420478848
1101	80-81	0.5411613352729923
1101	82-83	0.6187819061530817
1101	84-85	0.5641548684909878
1101	86-87	0.5556782571625654
1101	88-89	0.5006217626577225
1101	90-91	0.4314254787204419
1101	92-93	0.3982534800611113
1101	94-95	0.4499662484790896
1101	96-97	0.4436971044462119
1101	98-99	0.4636609720952478
1101	100	0.5200430863172443
1102	1	0.4391323937317715
1102	2	0.6220517840233306
1102	3	0.601058232459657
1102	4	0.7063131453472877
1102	5	0.5725945544826203
1102	6	0.6235921103254682
1102	7	0.6261627409253308
1102	8	0.6759202139988787
1102	9	0.7431592829176097
1102	10-11	0.7972432540393939
1102	12-13	0.8626613747821565
1102	14-15	0.9319047329747718
1102	16-17	0.8298895643855175
1102	18-19	0.6561124998249781
1102	20-21	0.7819360210522035
1102	22-23	0.680854730360501
1102	24-25	0.6518703599050468
1102	26-27	0.6890782829152968
1102	28-29	0.7151384257745761
1102	30-31	0.6800299034073944
1102	32-33	0.7707501837767694
1102	34-35	0.645832662409596
1102	36-37	0.6395050411060126
1102	38-39	0.5955611014639004
1102	40-41	0.6246844027691623
1102	42-43	0.7390552025712012
1102	44-45	0.6554399365387766
1102	46-47	0.7003746051722075
1102	48-49	0.6343545990281356
1102	50-51	0.6268558215197046
1102	52-53	0.6232762350136909
1102	54-55	0.6944739674433151
1102	56-57	0.7161246348240269
1102	58-59	0.7114446550210829
1102	60-61	0.7299035944867569
1102	62-63	0.7092908646787599
1102	64-65	0.6867044298537834
1102	66-67	0.7601805967457267
1102	68-69	0.7469479927077316
1102	70-71	0.7384281065448022
1102	72-73	0.8042138577610061
1102	74-75	0.7608242621717594
1102	76-77	0.6509874517340215
1102	78-79	0.7259458615391097
1102	80-81	0.7690426925723663
1102	82-83	0.7080300087610603
1102	84-85	0.6847691434210432
1102	86-87	0.7472262609164595
1102	88-89	0.7609776596069295
1102	90-91	0.7653378251336314
1102	92-93	0.7014367817874714
1102	94-95	0.6945029332458255
1102	96-97	0.807700978502659
1102	98-99	0.9295046891867713
1102	100	0.9399379571977917
1103	1	0.39594377367125233
1103	2	0.38398613010039995
1103	3	0.5443423682636634
1103	4	0.4820920139425411
1103	5	0.4370048347451956
1103	6	0.5290304168451812
1103	7	0.5029169775330544
1103	8	0.6115320823145112
1103	9	0.7326409231305675
1103	10-11	0.8121777634622376
1103	12-13	0.7092608758854269
1103	14-15	0.7925425566554765
1103	16-17	0.701461864266193
1103	18-19	0.5863383876368928
1103	20-21	0.5423177517974693
1103	22-23	0.5587083670186175
1103	24-25	0.604206896476505
1103	26-27	0.5463982495816779
1103	28-29	0.6326659799901435
1103	30-31	0.4981585146638494
1103	32-33	0.5885393680723254
1103	34-35	0.5621716685475207
1103	36-37	0.656936802720864
1103	38-39	0.6651804504618184
1103	40-41	0.4618912455724029
1103	42-43	0.41879728237805836
1103	44-45	0.3144526082940686
1103	46-47	0.3024607243813975
1103	48-49	0.28592230307884137
1103	50-51	0.22114619491705412
1103	52-53	0.25371834832028384
1103	54-55	0.32031139134218023
1103	56-57	0.23652956620495758
1103	58-59	0.26257187922283975
1103	60-61	0.3088308834730924
1103	62-63	0.3339809922268877
1103	64-65	0.30108384053548676
1103	66-67	0.3353991063125221
1103	68-69	0.317273327635764
1103	70-71	0.3204387544737699
1103	72-73	0.36533728901873985
1103	74-75	0.3187234141580717
1103	76-77	0.3455092777735551
1103	78-79	0.3023731297611576
1103	80-81	0.3246292561374027
1103	82-83	0.2993693609705197
1103	84-85	0.1800002776774221
1103	86-87	0.2120294015848465
1103	88-89	0.1942799849307839
1103	90-91	0.24729365533155345
1103	92-93	0.24980263200217223
1103	94-95	0.21533810075829152
1103	96-97	0.14459912549723697
1103	98-99	0.10778330168908923
1103	100	0.16082849615575512
1104	1	0.24048043993343526
1104	2	0.3482073006703139
1104	3	0.3848002414991143
1104	4	0.394408480390787
1104	5	0.30618414146363193
1104	6	0.49662820028569854
1104	7	0.3187338282388765
1104	8	0.22859635444977044
1104	9	0.41345150437071254
1104	10-11	0.28540916741805944
1104	12-13	0.366723157970938
1104	14-15	0.4352517747924338
1104	16-17	0.4380723079825728
1104	18-19	0.37221793299951145
1104	20-21	0.3688315243978906
1104	22-23	0.5026156598228866
1104	24-25	0.46883902040968195
1104	26-27	0.5522583798247709
1104	28-29	0.5505473050720582
1104	30-31	0.5151944730831417
1104	32-33	0.5779168708025821
1104	34-35	0.606824016563543
1104	36-37	0.5873837549515812
1104	38-39	0.6266535726557265
1104	40-41	0.5831281504787569
1104	42-43	0.5794420261389348
1104	44-45	0.5886733743823314
1104	46-47	0.44725544994059163
1104	48-49	0.35790143182534706
1104	50-51	0.3919426192992006
1104	52-53	0.5553516452502691
1104	54-55	0.4642623681653788
1104	56-57	0.48639568277657474
1104	58-59	0.4474102888009668
1104	60-61	0.42823305958440727
1104	62-63	0.5192950722333514
1104	64-65	0.6590347364623526
1104	66-67	0.6403871741036511
1104	68-69	0.5487767859539261
1104	70-71	0.49860645256865865
1104	72-73	0.5634491686450005
1104	74-75	0.5677393102871839
1104	76-77	0.5282015076809259
1104	78-79	0.6055272777205474
1104	80-81	0.655017963009545
1104	82-83	0.548047110434581
1104	84-85	0.6273666976102774
1104	86-87	0.5550395540744368
1104	88-89	0.6003682428282886
1104	90-91	0.5430247559261439
1104	92-93	0.5360033358002525
1104	94-95	0.5784938666443118
1104	96-97	0.5805437924065942
1104	98-99	0.674233166187733
1104	100	0.6257720709646364
1105	1	0.33980791960823353
1105	2	0.36925445514185995
1105	3	0.43578170491374735
1105	4	0.5840175860818491
1105	5	0.32468430406525783
1105	6	0.4272168181718783
1105	7	0.5991839095396898
1105	8	0.48684936257985356
1105	9	0.6339965450211125
1105	10-11	0.5975405495318853
1105	12-13	0.43089616610102155
1105	14-15	0.5795366528412131
1105	16-17	0.653693771397208
1105	18-19	0.6372650874710573
1105	20-21	0.5627619309019494
1105	22-23	0.5579704565708496
1105	24-25	0.5391232480519577
1105	26-27	0.5819832578735529
1105	28-29	0.6734572237712371
1105	30-31	0.6810780503189093
1105	32-33	0.7347897163310364
1105	34-35	0.6921378377017433
1105	36-37	0.7399431045450768
1105	38-39	0.7346379628996331
1105	40-41	0.6992959553568099
1105	42-43	0.6141737334560062
1105	44-45	0.5279202036506234
1105	46-47	0.6588170759568541
1105	48-49	0.6397459846708742
1105	50-51	0.6810496111691222
1105	52-53	0.5777048159819742
1105	54-55	0.6923456201978979
1105	56-57	0.6770480405001535
1105	58-59	0.680553703435109
1105	60-61	0.6577524091779026
1105	62-63	0.5065275925585588
1105	64-65	0.6394405901208913
1105	66-67	0.6460509139410462
1105	68-69	0.6218122331083968
1105	70-71	0.591133281836953
1105	72-73	0.6388911198645104
1105	74-75	0.6426414241083194
1105	76-77	0.5970327271931204
1105	78-79	0.5877165460132332
1105	80-81	0.6039571500014169
1105	82-83	0.5258773543370232
1105	84-85	0.5495026325696273
1105	86-87	0.5343397166760617
1105	88-89	0.5987032021778838
1105	90-91	0.48377922747085833
1105	92-93	0.5716287016539141
1105	94-95	0.6858044357500006
1105	96-97	0.6814166379350475
1105	98-99	0.7855255239113106
1105	100	0.7435867051109781
1106	1	0.17221585075263945
1106	2	0.16695059795346268
1106	3	0.25885608170686325
1106	4	0.3606996134965712
1106	5	0.20447171879271053
1106	6	0.23279361128631848
1106	7	0.2595511903812593
1106	8	0.296983477976724
1106	9	0.3710326432040958
1106	10-11	0.4347458284607981
1106	12-13	0.4044631664197951
1106	14-15	0.4008698501635024
1106	16-17	0.4427880143044405
1106	18-19	0.4144741407066235
1106	20-21	0.4413276433660869
1106	22-23	0.42249447314720356
1106	24-25	0.37483563568345346
1106	26-27	0.4072867197798331
1106	28-29	0.5700487188302077
1106	30-31	0.5136831124802157
1106	32-33	0.4280674211445472
1106	34-35	0.3397634672841505
1106	36-37	0.4389003248192225
1106	38-39	0.4286680808467125
1106	40-41	0.5230166566637848
1106	42-43	0.39716767671389874
1106	44-45	0.27895117591712193
1106	46-47	0.27503399658164795
1106	48-49	0.34281301686724674
1106	50-51	0.26454370106392844
1106	52-53	0.3267059763262523
1106	54-55	0.2808586708832692
1106	56-57	0.29887827320655447
1106	58-59	0.3536043377991547
1106	60-61	0.40781465973576303
1106	62-63	0.3596348925266888
1106	64-65	0.3283725378416449
1106	66-67	0.3828503528954599
1106	68-69	0.409577665049742
1106	70-71	0.3651290739950639
1106	72-73	0.4351155891026366
1106	74-75	0.49618231030532556
1106	76-77	0.49927609346663004
1106	78-79	0.3452287168771164
1106	80-81	0.38025205596252576
1106	82-83	0.3666547595011913
1106	84-85	0.3130334964523165
1106	86-87	0.3650325824714038
1106	88-89	0.3476006200930861
1106	90-91	0.4878394123608807
1106	92-93	0.4353046723265308
1106	94-95	0.4658156566617002
1106	96-97	0.4288709445974632
1106	98-99	0.471446390981761
1106	100	0.49721985780463385
1107	1	0.08558175799207035
1107	2	0.05246758645499128
1107	3	0.1134523109743526
1107	4	0.11217455577881452
1107	5	0.12291147578243056
1107	6	0.04366934342440487
1107	7	0.26239421256999407
1107	8	0.18039421106470144
1107	9	0.2838423025968737
1107	10-11	0.1935384788248129
1107	12-13	0.2710273277171851
1107	14-15	0.27709705688161534
1107	16-17	0.18529129664973
1107	18-19	0.3316044814104444
1107	20-21	0.20133799150801224
1107	22-23	0.2589255575809588
1107	24-25	0.3081436520923617
1107	26-27	0.3766226013078935
1107	28-29	0.3659715167005331
1107	30-31	0.3440733533492164
1107	32-33	0.4049002718865893
1107	34-35	0.35180399931791584
1107	36-37	0.34616254898951837
1107	38-39	0.39658074067740046
1107	40-41	0.3151816119224691
1107	42-43	0.42684040012267843
1107	44-45	0.35219995112537106
1107	46-47	0.3613532880780639
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2216	2	-1.0627936322898357
2216	3	-1.04152027791217
2216	4	-0.9757406028330635
2216	5	-1.306392542148636
2216	6	-1.143566007745374
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2216	12-13	-1.70239357628774
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2216	62-63	-1.93787615451404
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2216	84-85	-1.9962958373757296
2216	86-87	-1.9905894324262015
2216	88-89	-1.9277717392741103
2216	90-91	-1.9498441917328897
2216	92-93	-2.0232973248878494
2216	94-95	-1.9539788585513271
2216	96-97	-2.02227260484403
2216	98-99	-2.4292840935750277
2216	100	-2.4037875337648984
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92384.0
3	18265.0
4	13528.0
5	12493.0
6	8230.0
7	7143.0
8	7684.0
9	7957.0
10	8100.0
11	8252.0
12	9301.0
13	9950.0
14	10238.0
15	10322.0
16	9918.0
17	9723.0
18	9193.0
19	8854.0
20	8882.0
21	9111.0
22	11455.0
23	17966.0
24	27500.0
25	32959.0
26	33588.0
27	26605.0
28	27341.0
29	32051.0
30	37021.0
31	42456.0
32	53400.0
33	68368.0
34	91818.0
35	137826.0
36	208010.0
37	349512.0
38	388213.0
39	133764.0
40	318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.83924094378397	21.41793653659471	11.868668050691102	21.874154468930218
2	17.447342454956587	23.46323286344921	36.6653147100053	22.42410997158891
3	17.547394674472468	28.702565941449503	29.078302548737483	24.671736835340546
4	12.618125987717299	21.053431754986097	36.57334613614995	29.75509612114665
5	11.648970034503057	38.84191958822385	31.50955748167197	17.999552895601123
6	26.813966898708124	40.397419553598176	18.360399280019646	14.428214267674052
7	24.80523039776109	35.603653236591185	21.083928219562278	18.507188146085454
8	23.561807159382223	36.40764608978584	21.178680488387933	18.851866262444002
9	23.520536353706987	21.55677003524747	21.19689374147319	33.72579986957235
10-11	21.592730491577004	30.61280215644746	27.04306143524781	20.751405916727723
12-13	23.112186224967573	28.88844490709681	26.4675633376284	21.531805530307217
14-15	21.443196059937126	30.94710241542898	24.46705828576458	23.14264323886931
16-17	20.058371484589593	33.56827955900323	24.46491347172556	21.90843548468161
18-19	19.84209778794874	31.493549684135562	26.96925172735238	21.695100800563317
20-21	22.422208991828136	31.403810103447956	25.56843115726135	20.60554974746256
22-23	21.590824698418746	32.07967351233543	25.704027159628833	20.62547462961699
24-25	21.12983020290384	31.196331252492694	26.46399203837782	21.209846506225645
26-27	20.96781130475139	30.57878538736057	26.660995487061307	21.792407820826732
28-29	21.217067340995218	30.80046673511982	26.47021312670018	21.512252797184786
30-31	21.85084201924587	32.214625139653066	25.329316192554703	20.605216648546357
32-33	20.361337538510856	31.160680506611705	26.88514719329905	21.592834761578388
34-35	20.463451184020457	29.84474695396319	27.204952574419018	22.486849287597334
36-37	22.06533336700715	31.32479868852164	25.689202533237683	20.920665411233536
38-39	21.558395510305427	31.098296335544855	26.317743810454232	21.025564343695482
40-41	20.631231404088666	30.58712507179563	26.90966366014794	21.87197986396776
42-43	20.491387417555533	30.717464737384187	26.82299265829451	21.968155186765774
44-45	21.496137770038537	30.93889745161836	26.44444800067213	21.120516777670982
46-47	20.8565495219651	32.16115283489011	25.880973248299345	21.10132439484545
48-49	20.510944524737077	30.835629591474422	27.056005360991787	21.597420522796718
50-51	20.628534107213316	30.038042208998604	27.17442455345288	22.1589991303352
52-53	20.623244677020573	30.500837154036343	26.716190779103353	22.15972738983973
54-55	21.091157502923693	31.759385140256065	26.232958868739686	20.916498488080553
56-57	20.22991103912048	31.12310547432717	26.250810769722392	22.396172716829955
58-59	19.891916031263488	29.9995649236441	27.341223384704016	22.7672956603884
60-61	21.30609325986965	31.05420884639764	26.030423166314726	21.609274727417983
62-63	20.94252318863591	30.87437316606926	26.197938871267596	21.985164774027226
64-65	20.619396556574227	30.174996843130465	27.134550155468013	22.0710564448273
66-67	20.564797228298325	30.328589474694923	26.262928590074804	22.843684706931956
68-69	21.737737784793527	30.80832264593148	26.106574296175516	21.347365273099477
70-71	21.85498357678105	30.05006481270329	26.4665374413385	21.62841416917716
72-73	20.87125160747644	30.2243828328142	27.46271142237817	21.441654137331184
74-75	20.744746820187174	30.124382015616764	27.172784582871166	21.958086581324896
76-77	20.937230889867507	30.43308665633817	26.43142836282022	22.1982540909741
78-79	21.37411852486784	30.698263829676577	26.590105874533855	21.337511770921736
80-81	20.761155000027507	30.071028530935145	27.00944529932952	22.15837116970783
82-83	20.413644802560434	30.147112325204876	27.20426571984022	22.234977152394467
84-85	20.80092036129332	30.06356864040423	26.88926234322565	22.2462486550768
86-87	21.35732515658338	30.19903198229093	26.613035708436488	21.8306071526892
88-89	20.611898104830665	29.98575259931299	27.05358352882461	22.348765767031743
90-91	20.397007789079986	29.690894720273487	26.918446324709944	22.993651165936583
92-93	20.904522839235444	30.391903387141056	26.383303886528196	22.320269887095296
94-95	20.765724024498127	29.961323483006886	26.84589853390296	22.427053958592023
96-97	20.520220446208288	29.8723565345608	27.121879768752017	22.48554325047889
98-99	20.500538339633547	29.79538593175764	27.083559412642206	22.620516315966608
100	20.995597714191984	30.348267165689073	26.474375676058177	22.181759444060763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23913.0
1	17594.5
2	9373.0
3	6219.5
4	4135.5
5	2924.0
6	2417.5
7	2256.0
8	2256.0
9	2255.5
10	2012.5
11	1721.0
12	1610.0
13	1614.0
14	1727.5
15	1779.5
16	1874.0
17	2070.5
18	2278.5
19	2653.0
20	3193.0
21	3937.5
22	5126.0
23	7184.5
24	9579.5
25	11964.5
26	15374.5
27	19920.5
28	25775.5
29	31336.5
30	34736.5
31	38391.0
32	42580.5
33	46366.0
34	49780.5
35	52660.5
36	54953.5
37	56575.0
38	57859.5
39	58130.5
40	58877.5
41	58738.5
42	56888.5
43	56813.5
44	57099.5
45	56941.0
46	57876.5
47	59481.5
48	61605.5
49	63971.0
50	66034.0
51	65119.0
52	61348.0
53	57736.5
54	55235.0
55	53578.0
56	51142.0
57	47774.5
58	44611.5
59	41202.0
60	35812.0
61	29770.0
62	24290.0
63	19027.5
64	14416.0
65	10499.0
66	7537.5
67	5350.5
68	3778.5
69	2766.5
70	1983.0
71	1386.0
72	1058.0
73	839.5
74	642.5
75	490.0
76	357.0
77	276.0
78	221.5
79	174.0
80	142.0
81	106.0
82	70.5
83	58.5
84	58.5
85	54.5
86	38.0
87	35.0
88	36.0
89	28.5
90	18.0
91	15.0
92	16.0
93	12.5
94	10.0
95	9.5
96	11.0
97	11.0
98	12.5
99	14.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010801625644659522
2	0.00640096334498342
3	0.008001204181229276
4	0.006951046132442933
5	0.008301249338025372
6	0.010001505226536594
7	0.007851181602831226
8	0.007051061184708298
9	0.006150925714320006
10-11	0.005700857979125859
12-13	0.005875884320590249
14-15	0.0068510310801775665
16-17	0.004625696167273175
18-19	0.005350805296197077
20-21	0.004725711219538541
22-23	0.005675854216059517
24-25	0.005325801533130736
26-27	0.005750865505258542
28-29	0.013902092264885866
30-31	0.00505076013940098
32-33	0.005025756376334638
34-35	0.004625696167273175
36-37	0.006175929477386347
38-39	0.004875733797936589
40-41	0.004975748850201955
42-43	0.0047757187456712235
44-45	0.003850579512216589
46-47	0.005325801533130736
48-49	0.003450519303155125
50-51	0.0039005870383492713
52-53	0.0033505042508897593
54-55	0.004425666062742442
56-57	0.0028004214634302464
58-59	0.0025003763066341485
60-61	0.0035755381184868324
62-63	0.0033255004878234174
64-65	0.004575688641140492
66-67	0.003050459094093661
68-69	0.003525530592354149
70-71	0.00455068487807415
72-73	0.0029254402787619537
74-75	0.0026503988850321973
76-77	0.0026503988850321973
78-79	0.0034005117770224418
80-81	0.004000602090614638
82-83	0.0018252747038429282
84-85	0.002475372543567807
86-87	0.002275342439037075
88-89	0.001800270940776587
90-91	0.00202530480837366
92-93	0.0024003612543687823
94-95	0.001800270940776587
96-97	0.0013752069686487818
98-99	0.001600240836245855
100	0.0028004214634302464
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1999699.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.36361655623676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.65267191932271	63.69442451625663
2	10.193442823876005	15.16042547085277
3	2.1711622278948113	4.843664261896635
4	0.7124493743569248	2.1192124836163657
5	0.32958734986558363	1.2254653653595258
6	0.20977199194459534	0.9359642383923527
7	0.13367038063907768	0.6958149051539413
8	0.10063397211804989	0.5986804892094147
9	0.07237744144452382	0.4844023472611844
>10	0.38569627507136517	5.16674845887342
>50	0.02556947901820796	1.311210263601303
>100	0.012022040566502992	1.560097758386295
>500	6.073224953527374E-4	0.2798581801325001
>1k	2.699211090456429E-4	0.3865425070038603
>5k	0.0	0.0
>10k+	6.748027726141073E-5	1.5374887540038005
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30639	1.5321805931792734	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2940	0.14702212683008792	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2206	0.11031660264869862	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.5003763066341485E-5	0.0
14-15	0.0	0.0	0.0	7.501128919902446E-5	0.0
16-17	0.0	0.0	0.0	1.0001505226536594E-4	0.0
18-19	0.0	0.0	0.0	1.0001505226536594E-4	0.0
20-21	0.0	0.0	0.0	1.2501881533170744E-4	0.0
22-23	0.0	0.0	0.0	7.75116655056586E-4	0.0
24-25	0.0	0.0	0.0	0.00252538006970049	0.0
26-27	0.0	0.0	0.0	0.005250790243931712	0.0
28-29	0.0	0.0	0.0	0.010426569198664398	0.0
30-31	0.0	0.0	0.0	0.020628104529731725	0.0
32-33	0.0	0.0	0.0	0.04278143860651028	0.0
34-35	0.0	0.0	0.0	0.0738361123349064	0.0
36-37	0.0	0.0	0.0	0.11871786703898937	0.0
38-39	0.0	0.0	0.0	0.18322757575015042	0.0
40-41	0.0	0.0	0.0	0.27029067874715146	0.0
42-43	0.0	0.0	0.0	0.3645548655072588	0.0
44-45	0.0	0.0	0.0	0.4678454107343155	0.0
46-47	0.0	0.0	0.0	0.5855631272506512	0.0
48-49	0.0	0.0	0.0	0.7186581580527869	0.0
50-51	0.0	0.0	0.0	0.8611296000048008	0.0
52-53	0.0	0.0	0.0	1.0047762188209326	0.0
54-55	0.0	0.0	0.0	1.166025486835769	0.0
56-57	0.0	0.0	0.0	1.349528103979649	0.0
58-59	0.0	0.0	0.0	1.546507749416287	0.0
60-61	0.0	0.0	0.0	1.7553641823094375	0.0
62-63	0.0	0.0	0.0	1.965620825934303	0.0
64-65	0.0	0.0	0.0	2.214358260918268	0.0
66-67	0.0	0.0	0.0	2.508827578550572	0.0
68-69	0.0	0.0	0.0	2.8021467230818238	0.0
70-71	0.0	0.0	0.0	3.1341966966028387	2.5003763066341485E-5
72-73	0.0	0.0	0.0	3.5275809009255896	5.000752613268297E-5
74-75	0.0	0.0	0.0	3.913939047826698	5.000752613268297E-5
76-77	0.0	0.0	0.0	4.301322349013526	5.000752613268297E-5
78-79	0.0	0.0	0.0	4.737638014521186	5.000752613268297E-5
80-81	0.0	0.0	0.0	5.219510536335719	5.000752613268297E-5
82-83	0.0	0.0	0.0	5.727737024422176	5.000752613268297E-5
84-85	0.0	0.0	0.0	6.235813489930235	5.000752613268297E-5
86-87	0.0	0.0	0.0	6.765193161570817	5.000752613268297E-5
88	0.0	0.0	0.0	7.1925824836637915	5.000752613268297E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	2460	0.0	49.11119	2
GTACCTG	2695	0.0	45.17876	1
TCTGTCG	740	0.0	37.472775	8
GGTATCA	3590	0.0	35.355957	1
GTCTCGC	785	0.0	32.338314	1
CGCTCTG	785	0.0	31.738665	5
TCGCTCT	905	0.0	31.16629	4
ACCTGGG	3905	0.0	29.97511	3
TATAACG	160	2.1318556E-9	29.380724	2
GTATCAA	5240	0.0	28.439455	1
TCTCGCT	930	0.0	28.306587	2
CTGTCGC	1070	0.0	27.67275	9
GTACCCG	175	5.5460987E-9	26.863045	1
GTACACG	265	0.0	26.60962	1
CTCGCTC	990	0.0	26.116198	3
GTACATG	8170	0.0	25.37522	1
TACATGG	8140	0.0	24.832846	2
ATCAACG	5730	0.0	24.448042	3
TCAACGC	5740	0.0	24.405449	4
GGCTAGA	710	0.0	23.836224	1
>>END_MODULE