FastQCFastQC Report
Wed 25 May 2016
SRR2431455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999699
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196610.9831979712946798No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185220.9262393990295539No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177010.8851832200746211No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129530.6477474859966424No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102640.513277248225858No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41040.2052308872485309No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40950.20478081951333676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27040.13522035066277474No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76650.056.7263031
TATAACG3750.055.1901132
TACCTGG24550.050.5815662
ATAACGC4150.049.8705833
GTACCTG28900.043.3120231
GTATCAA134200.039.3077131
GTATAAC6700.035.8195461
ACCTGGG34500.034.76653
TAACGCA6400.034.5428164
GTCTCGC7350.030.7311331
GTACACG1701.200533E-1030.4486771
TATCAAC176850.029.8153822
ATCAACG176600.029.7776873
TCTGTCG6850.029.508438
TCAACGC178450.029.4426214
CGACTCA2101.8189894E-1229.100685
CAACGCA183750.028.6018125
GCGGTAT1704.0290615E-927.6806161
CGGTATC1704.0472514E-927.668842
GTGGTAT30900.027.5641611
Produced by FastQC (version 0.11.5)