##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431455_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1999699
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25344164296727	33.0	31.0	34.0	30.0	34.0
2	32.33175992986944	34.0	31.0	34.0	30.0	34.0
3	32.43610463374738	34.0	31.0	34.0	30.0	34.0
4	36.00958344230807	37.0	35.0	37.0	35.0	37.0
5	35.952852404286844	37.0	35.0	37.0	35.0	37.0
6	36.00635895702303	37.0	35.0	37.0	35.0	37.0
7	35.94329246551606	37.0	35.0	37.0	35.0	37.0
8	35.92413808278146	37.0	35.0	37.0	35.0	37.0
9	37.60023083474063	39.0	37.0	39.0	35.0	39.0
10-11	37.53707407964899	39.0	37.0	39.0	34.5	39.0
12-13	37.52984249129494	39.0	37.0	39.0	35.0	39.0
14-15	38.83119434474889	40.0	38.0	41.0	35.0	41.0
16-17	38.88354447344325	40.0	38.0	41.0	35.0	41.0
18-19	38.98213206087516	40.5	38.5	41.0	35.0	41.0
20-21	38.96623616854336	40.0	38.5	41.0	35.0	41.0
22-23	38.83913904042558	40.0	38.0	41.0	35.0	41.0
24-25	38.75336263107598	40.0	38.0	41.0	35.0	41.0
26-27	38.579160663679886	40.0	38.0	41.0	34.0	41.0
28-29	38.448479496164175	40.0	38.0	41.0	34.0	41.0
30-31	38.18374965432298	40.0	37.5	41.0	34.0	41.0
32-33	37.8519422173037	40.0	37.0	41.0	33.0	41.0
34-35	37.609511731515596	40.0	36.0	41.0	33.0	41.0
36-37	37.39756058286772	40.0	36.0	41.0	33.0	41.0
38-39	37.24569672735747	40.0	36.0	41.0	32.5	41.0
40-41	37.09468500009251	40.0	36.0	41.0	32.0	41.0
42-43	36.92689324743374	40.0	36.0	41.0	31.5	41.0
44-45	36.64025035767883	40.0	35.0	41.0	30.5	41.0
46-47	36.20776101803321	40.0	35.0	41.0	29.5	41.0
48-49	35.84572903221935	40.0	35.0	41.0	27.0	41.0
50-51	35.682616233743175	40.0	35.0	41.0	27.0	41.0
52-53	35.52941292664546	40.0	35.0	41.0	26.0	41.0
54-55	35.34860396489672	39.0	35.0	41.0	26.0	41.0
56-57	35.13769147256662	39.0	35.0	41.0	25.0	41.0
58-59	34.967115300852775	39.0	35.0	41.0	25.0	41.0
60-61	34.7207669754298	38.5	35.0	41.0	25.0	41.0
62-63	34.43443038177246	38.0	35.0	40.5	23.5	41.0
64-65	34.15080319588098	37.0	34.0	40.0	23.0	41.0
66-67	33.8635327116731	37.0	34.0	40.0	22.5	41.0
68-69	33.53877783606433	36.5	34.0	39.5	22.0	41.0
70-71	33.17004934242604	36.0	34.0	39.0	21.5	41.0
72-73	32.83042872952379	35.0	33.5	39.0	20.0	41.0
74-75	32.52942292815069	35.0	33.0	37.5	20.0	40.0
76-77	32.18882941882754	35.0	33.0	37.0	18.5	39.5
78-79	31.89233204597292	35.0	33.0	37.0	18.0	39.0
80-81	31.6062427395323	35.0	33.0	36.0	17.0	39.0
82-83	31.371827460032733	35.0	33.0	36.0	15.0	38.0
84-85	31.153766891917233	35.0	33.0	36.0	11.0	37.0
86-87	30.921996010399567	35.0	33.0	35.0	7.5	37.0
88-89	30.789377301283842	35.0	33.0	35.0	4.5	36.5
90-91	30.610841431635464	35.0	33.0	35.0	2.0	36.0
92-93	30.415294751860156	35.0	33.0	35.0	2.0	36.0
94-95	30.247946565958177	35.0	32.5	35.0	2.0	36.0
96-97	30.082932731376072	35.0	32.0	35.0	2.0	35.5
98-99	29.923261700885984	35.0	32.0	35.0	2.0	35.0
100	29.810691008996855	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16604823732608764
1101	2	0.19665667746453863
1101	3	0.1947037256408919
1101	4	0.17649617954539565
1101	5	0.14853661913539185
1101	6	0.13355743806189224
1101	7	0.16910752342647584
1101	8	0.2217669600597958
1101	9	0.26285458811229745
1101	10-11	0.23056168771928043
1101	12-13	0.2550954594225914
1101	14-15	0.41224226668854413
1101	16-17	0.3503332189137254
1101	18-19	0.34758214420240563
1101	20-21	0.31262300318834235
1101	22-23	0.34604646235447234
1101	24-25	0.30002546298523924
1101	26-27	0.2935155904264448
1101	28-29	0.3529918683710278
1101	30-31	0.3608282241056031
1101	32-33	0.389699145913994
1101	34-35	0.45069780585556884
1101	36-37	0.41017240712155
1101	38-39	0.3847258879328592
1101	40-41	0.40831221825162345
1101	42-43	0.31402807866215454
1101	44-45	0.35955793774750333
1101	46-47	0.37238982987145164
1101	48-49	0.4151728683723874
1101	50-51	0.3692951476913535
1101	52-53	0.369409547256474
1101	54-55	0.2994514467806866
1101	56-57	0.36997150562584835
1101	58-59	0.4163369837754871
1101	60-61	0.4651397052688466
1101	62-63	0.49038358605180576
1101	64-65	0.4129645256998913
1101	66-67	0.2965191409516308
1101	68-69	0.26474875262256603
1101	70-71	0.41952052543667406
1101	72-73	0.34409832617298264
1101	74-75	0.2356873175156764
1101	76-77	0.31608280417313495
1101	78-79	0.2773063116065373
1101	80-81	0.292394169401927
1101	82-83	0.25274972273976104
1101	84-85	0.2796451262498607
1101	86-87	0.26981089514929124
1101	88-89	0.20043175464028096
1101	90-91	0.23907971914690762
1101	92-93	0.2777006927322283
1101	94-95	0.2648900382290087
1101	96-97	0.28898138166669796
1101	98-99	0.33912606627572117
1101	100	0.3310740274979871
1102	1	0.23099286624499626
1102	2	0.3085461478080376
1102	3	0.25538911765546146
1102	4	0.2149247723516865
1102	5	0.2031569209935924
1102	6	0.15819240948766833
1102	7	0.19436554815835194
1102	8	0.24708640004612903
1102	9	0.2682490058884568
1102	10-11	0.2833421465424095
1102	12-13	0.30474201574577364
1102	14-15	0.5102053830454238
1102	16-17	0.42874508941431344
1102	18-19	0.4011478727102613
1102	20-21	0.4323650591196184
1102	22-23	0.439879736627546
1102	24-25	0.37824646778107507
1102	26-27	0.41227394532095474
1102	28-29	0.45409483961211095
1102	30-31	0.487123354443348
1102	32-33	0.5315774513647682
1102	34-35	0.7302553712525253
1102	36-37	0.7565261267219796
1102	38-39	0.7422493400487156
1102	40-41	0.8071729764073652
1102	42-43	0.786632257903463
1102	44-45	0.6974283971769992
1102	46-47	0.8164358601596362
1102	48-49	0.8699636754433797
1102	50-51	0.7814139795331627
1102	52-53	0.6752082821244869
1102	54-55	0.7688410047410557
1102	56-57	0.8811234366182461
1102	58-59	0.7794940060034676
1102	60-61	0.6716644641277512
1102	62-63	0.7150878165281256
1102	64-65	0.7559698344478392
1102	66-67	0.6597527653584052
1102	68-69	0.5676624169047528
1102	70-71	0.5677248246157518
1102	72-73	0.6637730144655336
1102	74-75	0.5543729643145454
1102	76-77	0.619899840119416
1102	78-79	0.6140150547689416
1102	80-81	0.6092005872960193
1102	82-83	0.5911897196467457
1102	84-85	0.6783129603997438
1102	86-87	0.6349243047584423
1102	88-89	0.5713332738333818
1102	90-91	0.5449272413139745
1102	92-93	0.6518404758540477
1102	94-95	0.6433297792390995
1102	96-97	0.651793499385942
1102	98-99	0.6763355885329112
1102	100	0.7092662717081808
1103	1	0.12264700512211846
1103	2	0.15046953033890276
1103	3	0.1778690939564811
1103	4	0.14414068499769428
1103	5	0.14443328818379797
1103	6	0.11272027101475857
1103	7	0.14605230081687637
1103	8	0.2028811602462426
1103	9	0.2767745473519767
1103	10-11	0.20297270825803793
1103	12-13	0.2192922473368526
1103	14-15	0.30402814458551575
1103	16-17	0.3275687230546893
1103	18-19	0.3029712544105507
1103	20-21	0.2699041305958474
1103	22-23	0.27327536283706166
1103	24-25	0.35371221085203075
1103	26-27	0.3747462249910498
1103	28-29	0.3995232346430768
1103	30-31	0.3845123139308342
1103	32-33	0.40451631852036485
1103	34-35	0.3489925146950199
1103	36-37	0.2550252836917224
1103	38-39	0.36620967194515686
1103	40-41	0.43295392143181743
1103	42-43	0.500634312184097
1103	44-45	0.4602816323921317
1103	46-47	0.48533344236546583
1103	48-49	0.48939185452569944
1103	50-51	0.442532150503574
1103	52-53	0.35762729446400954
1103	54-55	0.37841749624015364
1103	56-57	0.37319978276730126
1103	58-59	0.40786965696403144
1103	60-61	0.3862241088645533
1103	62-63	0.3448419396243807
1103	64-65	0.4800616735273451
1103	66-67	0.4175591701735044
1103	68-69	0.4050378657595388
1103	70-71	0.4368115003079609
1103	72-73	0.3321049773555913
1103	74-75	0.36921472656617027
1103	76-77	0.33669925982666626
1103	78-79	0.37408919713446664
1103	80-81	0.25990698851197536
1103	82-83	0.3554147824241234
1103	84-85	0.3121966859013412
1103	86-87	0.3172843465085151
1103	88-89	0.4027036894237668
1103	90-91	0.4405066619625444
1103	92-93	0.43775148840398614
1103	94-95	0.5266380569933453
1103	96-97	0.5160288058807367
1103	98-99	0.6086564549852902
1103	100	0.5574089749975215
1104	1	0.19273235075169737
1104	2	0.2446749261241976
1104	3	0.2290829434084074
1104	4	0.17373579885928336
1104	5	0.1331709009330595
1104	6	0.1717228192491973
1104	7	0.16715977159307727
1104	8	0.21550422379031176
1104	9	0.2851003977746629
1104	10-11	0.25090135769378463
1104	12-13	0.20180731427856813
1104	14-15	0.32630375838643744
1104	16-17	0.3373021696428822
1104	18-19	0.27886819248440986
1104	20-21	0.31601423050281596
1104	22-23	0.2783354283630999
1104	24-25	0.3160080487357888
1104	26-27	0.27436034949050025
1104	28-29	0.32868555313194037
1104	30-31	0.3726553386446554
1104	32-33	0.389347447428662
1104	34-35	0.38471714358310294
1104	36-37	0.3039732269024924
1104	38-39	0.40792791002306217
1104	40-41	0.38129271526752007
1104	42-43	0.3966695829845648
1104	44-45	0.42035848390691655
1104	46-47	0.44681141833925153
1104	48-49	0.39055028999065655
1104	50-51	0.39753922893256544
1104	52-53	0.41812642482845064
1104	54-55	0.4366434396936185
1104	56-57	0.37915294379144626
1104	58-59	0.3740339574129976
1104	60-61	0.27153090820120696
1104	62-63	0.3246275643463008
1104	64-65	0.3367983155206318
1104	66-67	0.2856536570885311
1104	68-69	0.31099507160025297
1104	70-71	0.30063061611106434
1104	72-73	0.3280278022554839
1104	74-75	0.3665653717144153
1104	76-77	0.25798146667859356
1104	78-79	0.26067982124966704
1104	80-81	0.20709300962591826
1104	82-83	0.27283338049069883
1104	84-85	0.3305528724482798
1104	86-87	0.23972498882538673
1104	88-89	0.14461041595372137
1104	90-91	0.06758994473566915
1104	92-93	0.0354459429604681
1104	94-95	0.0733128132931462
1104	96-97	0.16095701038666377
1104	98-99	0.14195823874939606
1104	100	0.21970562184458942
1105	1	0.13953430197121008
1105	2	0.18409606433558423
1105	3	0.17660554503442683
1105	4	0.16094360373732997
1105	5	0.13536927491679762
1105	6	0.14118753469634981
1105	7	0.12674351143047602
1105	8	0.17498650021307327
1105	9	0.17627438151449581
1105	10-11	0.1710093251734648
1105	12-13	0.04886942809969952
1105	14-15	0.1857066852157061
1105	16-17	0.21712786069979728
1105	18-19	0.23754266402912805
1105	20-21	0.16938008416135375
1105	22-23	0.2413103877126872
1105	24-25	0.23406723572765742
1105	26-27	0.22325010558805758
1105	28-29	0.2665053905303054
1105	30-31	0.21626899718124548
1105	32-33	0.16523167507094172
1105	34-35	0.21751974520911688
1105	36-37	0.22943403991062894
1105	38-39	0.19138937343769413
1105	40-41	0.2814904388447559
1105	42-43	0.3095619407081429
1105	44-45	0.353353605858139
1105	46-47	0.31010637768884664
1105	48-49	0.20620167210447704
1105	50-51	0.2665382533228069
1105	52-53	0.20783439230812206
1105	54-55	0.2796170982302044
1105	56-57	0.217352618588194
1105	58-59	0.28400338830731187
1105	60-61	0.3507309082012
1105	62-63	0.3027218732893857
1105	64-65	0.3596145756832314
1105	66-67	0.39578829123486514
1105	68-69	0.34645133176285015
1105	70-71	0.36218118521674825
1105	72-73	0.3016564201416614
1105	74-75	0.3984886237469354
1105	76-77	0.45541236098753046
1105	78-79	0.27347201637161334
1105	80-81	0.2379105706015281
1105	82-83	0.17963793333622746
1105	84-85	0.13856457976535097
1105	86-87	0.1698205985814809
1105	88-89	0.21998342408380012
1105	90-91	0.24240490408526583
1105	92-93	0.2618056177572221
1105	94-95	0.27273265069151975
1105	96-97	0.3549459534760935
1105	98-99	0.35374620622907216
1105	100	0.2730987925762953
1106	1	0.09245105045702218
1106	2	0.12095701428776806
1106	3	0.10542660974593332
1106	4	0.07806166557985961
1106	5	0.048337843613005305
1106	6	0.07849864722424371
1106	7	0.041060629696332285
1106	8	0.03628644283341487
1106	9	0.12932710704844652
1106	10-11	0.11779277432869861
1106	12-13	0.13911228433177314
1106	14-15	0.16623564919402867
1106	16-17	0.1090345741202583
1106	18-19	0.16182119765998948
1106	20-21	0.11995834364493874
1106	22-23	0.14797137591798304
1106	24-25	0.19955900493391
1106	26-27	0.16937080689503858
1106	28-29	0.17419778453731993
1106	30-31	0.17250963473305347
1106	32-33	0.2901416400055936
1106	34-35	0.28039775604749195
1106	36-37	0.35136903512898243
1106	38-39	0.2923630935524528
1106	40-41	0.3145940155103659
1106	42-43	0.35328350908557127
1106	44-45	0.2992351487334943
1106	46-47	0.3261764063786785
1106	48-49	0.26288344449848466
1106	50-51	0.2648300225290612
1106	52-53	0.24009594155899805
1106	54-55	0.23884392640999863
1106	56-57	0.3805758764778986
1106	58-59	0.37063948649028333
1106	60-61	0.3763252977453533
1106	62-63	0.2996213313703606
1106	64-65	0.3836943971687248
1106	66-67	0.3725301975147488
1106	68-69	0.37500304358943737
1106	70-71	0.3967506209834042
1106	72-73	0.4024399458031169
1106	74-75	0.3240925000618802
1106	76-77	0.32167439477778004
1106	78-79	0.3340906456352428
1106	80-81	0.3464484603050657
1106	82-83	0.31069364261260546
1106	84-85	0.2927477611488385
1106	86-87	0.27778061133251697
1106	88-89	0.28471574158459845
1106	90-91	0.38073242719651645
1106	92-93	0.3737257452675813
1106	94-95	0.31817474186142647
1106	96-97	0.32289744853506974
1106	98-99	0.39468962988224376
1106	100	0.423983127928544
1107	1	0.16231137267828188
1107	2	0.17186727645679412
1107	3	0.12732635311523666
1107	4	0.15678673505045992
1107	5	0.12822079006831189
1107	6	0.15825490843372592
1107	7	0.13121926900623038
1107	8	0.13522983354641127
1107	9	0.1244339774740979
1107	10-11	0.15862154739568268
1107	12-13	0.12454675133201931
1107	14-15	0.24779981652883976
1107	16-17	0.18543563847757127
1107	18-19	0.21715271453417984
1107	20-21	0.19021215486842635
1107	22-23	0.20536255942986514
1107	24-25	0.20877920542462647
1107	26-27	0.21769106518402026
1107	28-29	0.26959710770202605
1107	30-31	0.30761884566609154
1107	32-33	0.30110157406083715
1107	34-35	0.3245451492495164
1107	36-37	0.4296111611227502
1107	38-39	0.2739979087912303
1107	40-41	0.3493006913700114
1107	42-43	0.2971894154556125
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2216	70-71	-1.2671660747364086
2216	72-73	-1.1138525884453827
2216	74-75	-1.0716583864013849
2216	76-77	-1.0102144930327697
2216	78-79	-1.0548665316377495
2216	80-81	-1.0499710219043266
2216	82-83	-1.0099616919565122
2216	84-85	-1.2200971532557254
2216	86-87	-1.2496116574684777
2216	88-89	-1.0909309865329035
2216	90-91	-1.1698552676555671
2216	92-93	-1.0984795344676037
2216	94-95	-1.26334547577218
2216	96-97	-1.4025560605754315
2216	98-99	-1.5133156516944233
2216	100	-1.571667733575147
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	118.0
3	313.0
4	259.0
5	243.0
6	547.0
7	1239.0
8	1968.0
9	2843.0
10	3938.0
11	5728.0
12	8255.0
13	11937.0
14	10867.0
15	12161.0
16	15703.0
17	22656.0
18	25070.0
19	13973.0
20	9140.0
21	8295.0
22	8326.0
23	8559.0
24	9268.0
25	11023.0
26	13283.0
27	16619.0
28	20210.0
29	25553.0
30	31627.0
31	39692.0
32	49360.0
33	62892.0
34	86056.0
35	133019.0
36	192083.0
37	372221.0
38	535031.0
39	228179.0
40	1445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78590347425056	19.895265169139698	13.033767902331544	23.2850634542782
2	16.137278660438394	21.656559312176483	38.79768905220236	23.408472975182764
3	16.522844949587387	27.411689165063923	31.284073895158173	24.781391990190517
4	11.570029163389037	18.693892501786433	37.44748288127078	32.28859545355375
5	10.726225524639018	36.004116796600144	33.99257756375859	19.277080115002253
6	26.63890915582795	38.29546346725182	19.28995313794726	15.775674238972965
7	24.062371386893727	33.10048162248418	23.774027991212677	19.06311899940941
8	24.71756999428414	31.650263364636377	23.3032571401996	20.32890950087988
9	24.37946911010107	15.545989671445554	24.496836773934476	35.5777044445189
10-11	22.25999753943653	26.02898563771104	29.777919143483857	21.933097679368576
12-13	25.049694979094355	24.28975560821904	29.861244117239643	20.799305295446967
14-15	22.509337655317125	25.759651827600056	29.661314027761176	22.069696489321643
16-17	19.178036294462316	28.74337587806965	29.66346435138488	22.415123476083153
18-19	18.689112711463075	25.0767740544952	35.1062834956661	21.127829738375624
20-21	20.918423222695015	25.10005255790996	34.47106289496569	19.510461324429325
22-23	20.22366866213365	25.84388950537056	34.48739035224801	19.445051480247777
24-25	19.74445545448158	25.06267844949006	35.18034415825849	20.01252193776987
26-27	19.567669934325117	24.492061055188806	35.41855549260164	20.52171351788444
28-29	19.771404967811318	24.833505734753853	35.09170280345463	20.303386493980202
30-31	20.658239736430055	26.14820508486358	33.98484012720411	19.20871505150226
32-33	19.13936504412166	25.196129615571948	35.311206992255826	20.353298348050554
34-35	19.202719445309167	24.141353146577753	35.27990208396625	21.37602532414683
36-37	21.041270238169655	25.70126898793524	33.46820028755392	19.78926048634119
38-39	20.598534057386498	25.419431915982095	34.012329040456194	19.969704986175213
40-41	19.631991118578973	25.255390862538007	34.38032585213634	20.73229216674668
42-43	19.523604965634224	25.358660254922828	34.10795513646293	21.00977964298002
44-45	20.609451722484234	25.64273423150184	33.36939709426269	20.378416951751237
46-47	20.208102203816043	27.131098537498676	32.10679352629819	20.554005732387086
48-49	20.192934953151475	25.994835698242152	32.75345997466106	21.058769373945303
50-51	20.188966037671687	25.469008284610744	32.53739426700836	21.804631410709206
52-53	20.30194248045505	26.135959879691086	31.795949913123838	21.76614772673003
54-55	20.84230976956313	27.398983837413986	31.282905264842377	20.47580112818051
56-57	19.788120346362998	26.580726888191684	31.54069720193531	22.09045556351001
58-59	19.400418048973094	25.502337678445368	32.62599920531186	22.471245067269678
60-61	20.881597377976544	26.526984107933433	31.221717307345138	21.36970120674489
62-63	20.49124559954572	26.323236824016583	31.559524247100057	21.625993329337636
64-65	20.16882218037104	25.76534822106359	32.46472682101819	21.601102777547183
66-67	20.19719472710511	26.088332816172038	31.45001608992556	22.264456366797294
68-69	21.390663681863973	26.38764945787725	31.103516027895388	21.11817083236339
70-71	21.608566915021456	25.74295154306186	31.222617671812625	21.425863870104063
72-73	20.778327138234303	25.96970844112039	32.06392562080593	21.188038799839376
74-75	20.533657049771403	25.88390465033999	31.732828922446828	21.84960937744178
76-77	20.74562221614353	26.394347349276064	30.81851318623453	22.041517248345876
78-79	21.204307872028036	26.71619272606527	30.88036733730462	21.19913206460208
80-81	20.66751046032428	26.020766125301858	31.260979777456505	22.050743636917357
82-83	20.228186795120713	26.149041532947187	31.40851462298296	22.214257048949143
84-85	20.648281456047872	26.25373370932551	30.93869190528318	22.15929292934344
86-87	20.959079341440887	26.423276703143824	30.593304292295993	22.0243396631193
88-89	20.09142375927577	26.316835683770407	30.983012943447992	22.608727613505835
90-91	19.82708397613841	26.00083812613798	30.830690018847832	23.34138787887577
92-93	20.353414072353072	26.682057354775278	30.307587060820307	22.656941512051343
94-95	20.187638239555053	26.196217530738373	30.81391249383032	22.802231735876248
96-97	19.847391421974695	26.287486416651372	30.905249861103538	22.959872300270394
98-99	19.907185613770135	26.114997824662822	30.801324205251813	23.17649235631523
100	20.334661203164085	26.539510545113405	30.114957588511043	23.01087066321147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1988.0
1	1294.5
2	6396.0
3	11485.5
4	6891.0
5	2818.0
6	3836.5
7	7925.5
8	12004.0
9	12598.0
10	8704.5
11	4482.5
12	3524.0
13	3622.0
14	3886.5
15	4102.0
16	4269.5
17	4647.0
18	5013.0
19	5229.0
20	5728.0
21	6297.5
22	7295.5
23	9162.5
24	11120.0
25	13268.5
26	16532.5
27	20870.5
28	26438.0
29	31394.0
30	34471.5
31	38021.5
32	42039.0
33	45532.0
34	49040.5
35	51909.5
36	53723.5
37	55232.0
38	56285.0
39	56455.0
40	57128.0
41	57298.0
42	55732.0
43	55466.5
44	55675.5
45	55352.5
46	56421.5
47	58741.5
48	61442.0
49	63975.5
50	66105.5
51	64849.0
52	60326.0
53	56081.5
54	53417.0
55	52259.0
56	49802.0
57	46127.0
58	43285.5
59	40144.5
60	34888.5
61	28760.5
62	22998.0
63	17627.5
64	12995.0
65	9026.5
66	6259.0
67	4294.0
68	2849.0
69	1913.5
70	1214.0
71	810.0
72	570.0
73	393.0
74	305.5
75	230.5
76	154.5
77	105.0
78	61.5
79	35.5
80	19.0
81	5.0
82	3.0
83	2.0
84	3.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	1.0
95	1.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.046857051986323936
2	0.0
3	5.000752613268297E-5
4	0.06515980655088591
5	0.0043006472474107355
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.007851181602831226
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.250639721278052E-4
26-27	0.0
28-29	2.7504139372975633E-4
30-31	0.0039005870383492713
32-33	5.000752613268297E-5
34-35	0.0013251994425160986
36-37	0.0037005569338185397
38-39	0.001500225783980489
40-41	9.501429965209764E-4
42-43	0.0017502634146439038
44-45	0.0
46-47	4.250639721278052E-4
48-49	4.750714982604882E-4
50-51	0.0013752069686487818
52-53	6.751016027912202E-4
54-55	9.501429965209764E-4
56-57	0.006025906898988298
58-59	0.008626298257887813
60-61	0.0011001655749190253
62-63	0.0023753574913024413
64-65	0.0010501580487863424
66-67	2.5003763066341485E-5
68-69	6.751016027912202E-4
70-71	3.000451567960978E-4
72-73	0.0
74-75	3.250489198624393E-4
76-77	0.0
78-79	5.500827874595127E-4
80-81	0.0
82-83	0.0010751618118526839
84-85	6.000903135921956E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.500376306634149E-4
94-95	0.0
96-97	4.0006020906146377E-4
98-99	4.5006773519414673E-4
100	2.500376306634149E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1999699.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.0030451539709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.17410274732774	56.56092264767544
2	11.812999104003184	16.066398229466927
3	2.5706977005016682	5.244458154132723
4	0.853279629730984	2.321024527582387
5	0.4086764320232515	1.3895620930120445
6	0.25346057387041315	1.0341654509796647
7	0.15853291729119834	0.7546504793060866
8	0.11792096830501861	0.6415187945796922
9	0.0916194426515897	0.5607360986055931
>10	0.5034761203885095	6.232933095345886
>50	0.034985267390848424	1.6484315765095847
>100	0.018114743170292543	2.223773324195845
>500	0.001251172650795009	0.5789184858490116
>1k	5.151887385626456E-4	0.7835694488157737
>5k	0.0	0.0
>10k+	3.6799195611617546E-4	3.958937593943314
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19661	0.9831979712946798	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18522	0.9262393990295539	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17701	0.8851832200746211	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12953	0.6477474859966424	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10264	0.513277248225858	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4104	0.2052308872485309	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4095	0.20478081951333676	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2704	0.13522035066277474	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.000752613268297E-5	0.0
5	0.0	0.0	0.0	1.0001505226536594E-4	0.0
6	0.0	0.0	0.0	1.0001505226536594E-4	0.0
7	0.0	0.0	0.0	1.0001505226536594E-4	0.0
8	0.0	0.0	0.0	1.0001505226536594E-4	0.0
9	0.0	0.0	0.0	1.0001505226536594E-4	0.0
10-11	0.0	0.0	0.0	1.0001505226536594E-4	0.0
12-13	0.0	0.0	0.0	1.0001505226536594E-4	0.0
14-15	0.0	0.0	0.0	1.750263414643904E-4	0.0
16-17	0.0	0.0	0.0	2.0003010453073188E-4	0.0
18-19	0.0	0.0	0.0	2.2503386759707337E-4	0.0
20-21	0.0	0.0	0.0	3.000451567960978E-4	0.0
22-23	0.0	0.0	0.0	9.751467595873178E-4	0.0
24-25	0.0	0.0	0.0	0.0027254101742312216	0.0
26-27	0.0	0.0	0.0	0.005550835400727809	0.0
28-29	0.0	0.0	0.0	0.011076667038389276	0.0
30-31	0.0	0.0	0.0	0.021828285156916115	0.0
32-33	0.0	0.0	0.0	0.04475673588875126	0.0
34-35	0.0	0.0	0.0	0.07701159024433177	0.0
36-37	0.0	0.0	0.0	0.12301851428640011	0.0
38-39	0.0	0.0	0.0	0.18940350522753674	0.0
40-41	0.0	0.0	0.0	0.27999213881689194	0.0
42-43	0.0	0.0	0.0	0.37800689003695054	0.0
44-45	0.0	0.0	0.0	0.4844979169364989	0.0
46-47	0.0	0.0	0.0	0.6056161452298572	0.0
48-49	0.0	0.0	0.0	0.7434118834884651	0.0
50-51	0.0	0.0	0.0	0.8904590140816193	0.0
52-53	0.0	0.0	0.0	1.038781336591157	0.0
54-55	0.0	0.0	0.0	1.205006353456195	0.0
56-57	0.0	0.0	0.0	1.3958600769415797	0.0
58-59	0.0	0.0	0.0	1.6000408061413243	0.0
60-61	0.0	0.0	0.0	1.8164233717174434	0.0
62-63	0.0	0.0	0.0	2.033331016317956	0.0
64-65	0.0	0.0	0.0	2.2908197683751403	0.0
66-67	0.0	0.0	0.0	2.5945404783419903	0.0
68-69	0.0	0.0	0.0	2.89573580823914	0.0
70-71	0.0	0.0	0.0	3.237312215488431	0.0
72-73	0.0	0.0	0.0	3.6422731621108975	0.0
74-75	0.0	0.0	0.0	4.038507795423211	0.0
76-77	0.0	2.5003763066341485E-5	0.0	4.435642564205914	0.0
78-79	0.0	5.000752613268297E-5	0.0	4.884235077379145	0.0
80-81	0.0	5.000752613268297E-5	0.0	5.381759954873209	0.0
82-83	0.0	5.000752613268297E-5	0.0	5.90683897926638	0.0
84-85	0.0	5.000752613268297E-5	0.0	6.433718274600327	0.0
86-87	0.0	5.000752613268297E-5	0.0	6.981475712094671	0.0
88	0.0	5.000752613268297E-5	0.0	7.424317359762644	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7665	0.0	56.726303	1
TATAACG	375	0.0	55.190113	2
TACCTGG	2455	0.0	50.581566	2
ATAACGC	415	0.0	49.870583	3
GTACCTG	2890	0.0	43.312023	1
GTATCAA	13420	0.0	39.307713	1
GTATAAC	670	0.0	35.819546	1
ACCTGGG	3450	0.0	34.7665	3
TAACGCA	640	0.0	34.542816	4
GTCTCGC	735	0.0	30.731133	1
GTACACG	170	1.200533E-10	30.448677	1
TATCAAC	17685	0.0	29.815382	2
ATCAACG	17660	0.0	29.777687	3
TCTGTCG	685	0.0	29.50843	8
TCAACGC	17845	0.0	29.442621	4
CGACTCA	210	1.8189894E-12	29.10068	5
CAACGCA	18375	0.0	28.601812	5
GCGGTAT	170	4.0290615E-9	27.680616	1
CGGTATC	170	4.0472514E-9	27.66884	2
GTGGTAT	3090	0.0	27.564161	1
>>END_MODULE