FastQCFastQC Report
Wed 25 May 2016
SRR2431454_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431454_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4777075
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA389060.8144314250875273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89550.044.988231
GTATCAA143050.034.866731
ATCAACG172200.028.1713393
TCAACGC171750.028.1627454
TATCAAC179600.027.3773142
GTACATG302900.026.3059581
TACATGG305350.025.478012
CATGGGT111700.024.1975294
ACATGGG311800.023.9858423
CAACGCA204200.023.6873255
GTGGTAT45750.023.530331
TGGTATC46150.023.0198352
AACGCAG213200.022.6850176
ACGCAGA218450.021.9244387
CGCAGAG214250.021.9145648
AATGCGG12400.020.0891027
TAGTACG2351.2655073E-720.0031132
ATGCGGG12750.019.5368218
GCAGAGT225650.019.411939
ATGGGTA50000.018.8965455
Produced by FastQC (version 0.11.5)