##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431454_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4777075
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.89279883610787	31.0	30.0	34.0	25.0	34.0
2	29.9353951947583	31.0	31.0	34.0	25.0	34.0
3	29.937477012607086	31.0	31.0	34.0	25.0	34.0
4	33.17409398010289	37.0	35.0	37.0	25.0	37.0
5	33.388361706692905	37.0	35.0	37.0	27.0	37.0
6	33.29548709199667	37.0	35.0	37.0	26.0	37.0
7	33.225433973718225	37.0	35.0	37.0	26.0	37.0
8	33.12178896081807	37.0	35.0	37.0	25.0	37.0
9	34.35171229256397	39.0	35.0	39.0	22.0	39.0
10-11	34.35558390856329	39.0	35.0	39.0	23.0	39.0
12-13	34.32184788809052	39.0	35.0	39.0	22.5	39.0
14-15	35.4787734126008	40.0	36.0	41.0	23.0	41.0
16-17	35.51096225200567	40.0	36.0	41.0	23.5	41.0
18-19	35.50715908793561	40.0	36.0	41.0	24.0	41.0
20-21	35.621554193727334	40.0	36.0	41.0	25.0	41.0
22-23	35.599200033493304	40.0	36.0	41.0	25.0	41.0
24-25	35.64698021278711	40.0	36.0	41.0	25.5	41.0
26-27	35.66020797245176	40.0	35.0	41.0	26.0	41.0
28-29	35.56327794727945	40.0	35.0	41.0	26.0	41.0
30-31	35.544319379536645	40.0	35.0	41.0	26.0	41.0
32-33	35.486303962152576	40.0	35.0	41.0	26.0	41.0
34-35	35.374373021147875	40.0	35.0	41.0	25.0	41.0
36-37	35.2355243532915	40.0	35.0	41.0	25.0	41.0
38-39	35.1248328736727	40.0	35.0	41.0	25.0	41.0
40-41	35.017484757932415	40.0	35.0	41.0	24.5	41.0
42-43	34.77326146229649	39.0	34.0	41.0	24.0	41.0
44-45	34.61835820036319	39.0	34.0	41.0	23.0	41.0
46-47	34.46242826415746	39.0	34.0	41.0	23.0	41.0
48-49	34.31083079080818	39.0	33.0	41.0	22.5	41.0
50-51	34.1197313209443	38.5	33.0	40.0	22.0	41.0
52-53	33.996408995044035	38.0	33.0	40.0	22.0	41.0
54-55	33.913199081027614	38.0	33.0	40.0	21.0	41.0
56-57	33.72717845124893	38.0	33.0	40.0	20.0	41.0
58-59	33.50305040218125	37.5	33.0	40.0	19.0	41.0
60-61	33.276798563974815	37.0	33.0	40.0	18.0	41.0
62-63	32.989997121669646	37.0	32.0	40.0	15.0	41.0
64-65	32.834291171899125	36.0	32.0	40.0	13.0	41.0
66-67	32.568306128750336	36.0	32.0	39.5	9.0	41.0
68-69	32.29739150002878	35.5	32.0	39.0	7.5	41.0
70-71	32.010325879329926	35.0	31.5	39.0	4.5	41.0
72-73	31.70071979192288	35.0	31.0	38.5	2.0	40.5
74-75	31.365634619510892	35.0	31.0	37.5	2.0	40.0
76-77	31.053903382299836	35.0	31.0	37.0	2.0	39.5
78-79	30.73416264136527	35.0	31.0	36.5	2.0	39.0
80-81	30.45807727532015	35.0	30.5	36.0	2.0	39.0
82-83	30.177747156994606	35.0	30.0	36.0	2.0	37.5
84-85	29.90962293872296	35.0	30.0	35.0	2.0	37.0
86-87	29.671330468958516	35.0	30.0	35.0	2.0	37.0
88-89	29.422515242067583	34.5	30.0	35.0	2.0	36.0
90-91	29.207581731498877	34.0	30.0	35.0	2.0	36.0
92-93	29.034579737600936	34.0	30.0	35.0	2.0	36.0
94-95	28.815109245720446	34.0	29.0	35.0	2.0	35.5
96-97	28.73968746565629	34.0	29.0	35.0	2.0	35.0
98-99	28.594643793534747	34.0	29.0	35.0	2.0	35.0
100	28.461443875174663	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.41821516659802427
1101	2	0.4791926313374546
1101	3	0.49948715318173953
1101	4	0.3829061143177981
1101	5	0.37197578131547715
1101	6	0.4255470133419621
1101	7	0.436776074378848
1101	8	0.4449843622113434
1101	9	0.5648896607737157
1101	10-11	0.6281321533522686
1101	12-13	0.5675787192245707
1101	14-15	0.7166813617221877
1101	16-17	0.6198157376624707
1101	18-19	0.5643229109168146
1101	20-21	0.5374019443218714
1101	22-23	0.47476917084956227
1101	24-25	0.498623127835522
1101	26-27	0.5531619819972633
1101	28-29	0.6293032434319557
1101	30-31	0.6107146292644643
1101	32-33	0.6045601077496272
1101	34-35	0.6253190531882566
1101	36-37	0.6627817016251498
1101	38-39	0.6327135690708516
1101	40-41	0.6300506801932855
1101	42-43	0.622409362228467
1101	44-45	0.5500081765375526
1101	46-47	0.5535953635330415
1101	48-49	0.5336480877392873
1101	50-51	0.4098331776774131
1101	52-53	0.4381432236116112
1101	54-55	0.44605777289922344
1101	56-57	0.4817668258437351
1101	58-59	0.4515129777317668
1101	60-61	0.43786663969037676
1101	62-63	0.41175365840610567
1101	64-65	0.3946712606542988
1101	66-67	0.47819197061815544
1101	68-69	0.411952146246513
1101	70-71	0.4399286190791116
1101	72-73	0.44004414045028994
1101	74-75	0.4250500940200652
1101	76-77	0.42732795231884424
1101	78-79	0.3743348790112293
1101	80-81	0.41284649861869127
1101	82-83	0.41253885271841995
1101	84-85	0.398428885734063
1101	86-87	0.36543012854738777
1101	88-89	0.4019529375909059
1101	90-91	0.3866870407174332
1101	92-93	0.3458180110859068
1101	94-95	0.42789997247903955
1101	96-97	0.44607349062607327
1101	98-99	0.4106172641323198
1101	100	0.3689877174432432
1102	1	0.26868475575946604
1102	2	0.3950629766869085
1102	3	0.3382149898367892
1102	4	0.33806169037784883
1102	5	0.36936996307688474
1102	6	0.23080452429236686
1102	7	0.3953980951960361
1102	8	0.4238205087171849
1102	9	0.5396442961326997
1102	10-11	0.48773637352942956
1102	12-13	0.5087467650748962
1102	14-15	0.5922595426484207
1102	16-17	0.5256822171547029
1102	18-19	0.5281983751660775
1102	20-21	0.5152400055107904
1102	22-23	0.5364760304963099
1102	24-25	0.5170335183488319
1102	26-27	0.5537260157792048
1102	28-29	0.627369560305759
1102	30-31	0.5730251278379441
1102	32-33	0.6798507638300464
1102	34-35	0.6517971228167525
1102	36-37	0.5930006123067031
1102	38-39	0.5043171741130408
1102	40-41	0.6112487310220374
1102	42-43	0.6699852653444367
1102	44-45	0.4474974545454984
1102	46-47	0.463931551811541
1102	48-49	0.40839451946938965
1102	50-51	0.3746873156468098
1102	52-53	0.41490675346026507
1102	54-55	0.43301300838520973
1102	56-57	0.5305890495018275
1102	58-59	0.464247019512932
1102	60-61	0.4326678299573601
1102	62-63	0.5131513688254117
1102	64-65	0.4756847709884724
1102	66-67	0.5489654178035934
1102	68-69	0.5320062874534628
1102	70-71	0.5379016916961774
1102	72-73	0.5848668961095953
1102	74-75	0.5740674693883534
1102	76-77	0.5414489355415988
1102	78-79	0.49670407605842115
1102	80-81	0.49565733075612073
1102	82-83	0.47557897353948775
1102	84-85	0.5089316296284281
1102	86-87	0.4932337495852792
1102	88-89	0.46479029982516096
1102	90-91	0.4523636404970901
1102	92-93	0.4584569566205339
1102	94-95	0.4947265893313002
1102	96-97	0.4227608831163536
1102	98-99	0.4432235680037877
1102	100	0.3730627809219058
1103	1	0.12487448110606536
1103	2	0.24273067452049446
1103	3	0.24557567217865994
1103	4	0.20275356127534394
1103	5	0.1402116606412278
1103	6	0.15245435071621216
1103	7	0.20115256241887636
1103	8	0.2670280418070661
1103	9	0.258614087081817
1103	10-11	0.3615249931915159
1103	12-13	0.32255864044715565
1103	14-15	0.43752594127973055
1103	16-17	0.31128296897563956
1103	18-19	0.31999041417218876
1103	20-21	0.36660751558354576
1103	22-23	0.2969507649185843
1103	24-25	0.3522781599280833
1103	26-27	0.35391293715059646
1103	28-29	0.4040978650313818
1103	30-31	0.292278015836537
1103	32-33	0.3624316819799347
1103	34-35	0.3733842083954855
1103	36-37	0.3459772513104937
1103	38-39	0.3734807854744062
1103	40-41	0.396565491105207
1103	42-43	0.3133275186096185
1103	44-45	0.29692364565507035
1103	46-47	0.2957991648065885
1103	48-49	0.19955335111870198
1103	50-51	0.14131614693957317
1103	52-53	0.19277660313186828
1103	54-55	0.28909820983875534
1103	56-57	0.2067765030028852
1103	58-59	0.25446743359588453
1103	60-61	0.27748829826946775
1103	62-63	0.19019941305668198
1103	64-65	0.24181693982203
1103	66-67	0.23813828885744925
1103	68-69	0.17071100678179363
1103	70-71	0.2319574621904792
1103	72-73	0.19564241810940786
1103	74-75	0.15759577849684092
1103	76-77	0.182339875424244
1103	78-79	0.19047493803558524
1103	80-81	0.201081452551076
1103	82-83	0.17771845160087096
1103	84-85	0.16541460066160596
1103	86-87	0.07094443089549785
1103	88-89	0.19320889386028028
1103	90-91	0.17973999998699952
1103	92-93	0.1706563540556978
1103	94-95	0.14097915316553866
1103	96-97	0.17589297697281836
1103	98-99	0.2236224916208691
1103	100	0.24633042690333795
1104	1	0.18353727986536228
1104	2	0.2605481129381211
1104	3	0.17099317524472113
1104	4	0.2464400680747616
1104	5	0.19525322864043915
1104	6	0.18490192027908137
1104	7	0.2186478642032057
1104	8	0.21016454792827943
1104	9	0.3343302915111934
1104	10-11	0.32060619964423154
1104	12-13	0.2785122918059457
1104	14-15	0.2852113984988094
1104	16-17	0.32132533189886914
1104	18-19	0.23559094578267548
1104	20-21	0.14188444104620146
1104	22-23	0.19467319173848097
1104	24-25	0.21743472466744862
1104	26-27	0.3015367245087788
1104	28-29	0.36579057440669516
1104	30-31	0.3187769854968039
1104	32-33	0.2923702276988749
1104	34-35	0.2811687171016146
1104	36-37	0.3128667525348945
1104	38-39	0.33728201007835423
1104	40-41	0.3085864728472103
1104	42-43	0.23116684879673244
1104	44-45	0.22309094767275184
1104	46-47	0.188864036346736
1104	48-49	0.18866081919066602
1104	50-51	0.10341615122990788
1104	52-53	0.12205102905917187
1104	54-55	0.03025310155963723
1104	56-57	0.07387428537535357
1104	58-59	0.06999546107750376
1104	60-61	0.07156803627068342
1104	62-63	0.07531930711557067
1104	64-65	0.0996548672520774
1104	66-67	0.1080330918572514
1104	68-69	0.1253633447747191
1104	70-71	0.006783976773732547
1104	72-73	-0.014976697374663672
1104	74-75	0.03440686691939021
1104	76-77	-0.011477354812154061
1104	78-79	0.02828432006093351
1104	80-81	0.011945727364579994
1104	82-83	-0.10145151803638797
1104	84-85	0.018449764707007432
1104	86-87	0.04462407996046025
1104	88-89	0.02352118993377772
1104	90-91	0.0031828684215255976
1104	92-93	-0.02560703745881554
1104	94-95	0.020099783937642002
1104	96-97	0.039407765302136966
1104	98-99	0.07820648188091184
1104	100	0.03138210079136172
1105	1	0.21990039404363415
1105	2	0.31166167134962564
1105	3	0.2785439004812993
1105	4	0.24307087926531068
1105	5	0.23852286788172705
1105	6	0.26021428855875683
1105	7	0.2762125569591021
1105	8	0.291501173408669
1105	9	0.3602007820273272
1105	10-11	0.44726595831194516
1105	12-13	0.3191214698711491
1105	14-15	0.42135510278410493
1105	16-17	0.43867972670857824
1105	18-19	0.37135531621120776
1105	20-21	0.4244693412486953
1105	22-23	0.3786168051109371
1105	24-25	0.4808288938479919
1105	26-27	0.5009143020946283
1105	28-29	0.5598092528644756
1105	30-31	0.5249693074935777
1105	32-33	0.5508161552165376
1105	34-35	0.5221083427059767
1105	36-37	0.5108036094298072
1105	38-39	0.48699966370040215
1105	40-41	0.46788720841479403
1105	42-43	0.482532669698422
1105	44-45	0.38487870749753483
1105	46-47	0.39965264331396355
1105	48-49	0.3972068128267665
1105	50-51	0.46658192254277253
1105	52-53	0.36772469326016477
1105	54-55	0.4522525850093686
1105	56-57	0.470621382362836
1105	58-59	0.41215530239325915
1105	60-61	0.3968778217990021
1105	62-63	0.4016699827633303
1105	64-65	0.4556708803104641
1105	66-67	0.4774364556326134
1105	68-69	0.35925071615370285
1105	70-71	0.4063109406978569
1105	72-73	0.3747898425650007
1105	74-75	0.3399774483483746
1105	76-77	0.33780794623334387
1105	78-79	0.27010553168124574
1105	80-81	0.29815503353425754
1105	82-83	0.3284131871167162
1105	84-85	0.27905728981134814
1105	86-87	0.26764540315563323
1105	88-89	0.3440087720652727
1105	90-91	0.30123671362174065
1105	92-93	0.28723212944890975
1105	94-95	0.33536498084660593
1105	96-97	0.3995168193952807
1105	98-99	0.39240107669888147
1105	100	0.41435862874705975
1106	1	0.05804505925045689
1106	2	0.10726544417643069
1106	3	0.06151073255233541
1106	4	0.08424276587960833
1106	5	0.06859527079873828
1106	6	0.15325059046526235
1106	7	0.14776801429884046
1106	8	0.13232936715277077
1106	9	0.19147988683477024
1106	10-11	0.18802959412768416
1106	12-13	0.15864405058111686
1106	14-15	0.20594126707685234
1106	16-17	0.1540210088268239
1106	18-19	0.06890285824869125
1106	20-21	0.1388332844353073
1106	22-23	0.15667726476097243
1106	24-25	0.19548553245618194
1106	26-27	0.23815993762911347
1106	28-29	0.36952088675161576
1106	30-31	0.3347548711072932
1106	32-33	0.29363494702801773
1106	34-35	0.20631783705075435
1106	36-37	0.19604876281389494
1106	38-39	0.2247469666716455
1106	40-41	0.2856760365884554
1106	42-43	0.232871490840715
1106	44-45	0.183396175404404
1106	46-47	0.1347282706117099
1106	48-49	0.1250579589384202
1106	50-51	0.14930656444565216
1106	52-53	0.18006953123754244
1106	54-55	0.2501340061476469
1106	56-57	0.17738938766648005
1106	58-59	0.202699168034016
1106	60-61	0.15598555227693822
1106	62-63	0.19906074556542563
1106	64-65	0.23479528515608195
1106	66-67	0.25333162810181875
1106	68-69	0.2028786668136533
1106	70-71	0.17495069399757313
1106	72-73	0.18155317703674712
1106	74-75	0.18894770569614394
1106	76-77	0.24755210518225468
1106	78-79	0.18422099665502856
1106	80-81	0.15837728477479018
1106	82-83	0.07916437742549576
1106	84-85	0.12637423337966425
1106	86-87	0.12992740086541943
1106	88-89	0.2201922101993503
1106	90-91	0.19334722650921776
1106	92-93	0.18261885424148616
1106	94-95	0.2504491060449894
1106	96-97	0.2820957815665679
1106	98-99	0.3106768868119829
1106	100	0.33029114778195634
1107	1	0.008112937597640979
1107	2	0.09618780375380709
1107	3	0.0625857123279907
1107	4	0.09116495592036244
1107	5	0.1155054462789451
1107	6	0.16512021190370518
1107	7	0.17062370678488747
1107	8	0.13489838595221926
1107	9	0.23455618272419088
1107	10-11	0.28558128932240123
1107	12-13	0.17055178345999877
1107	14-15	0.23052060801059326
1107	16-17	0.19001422148209457
1107	18-19	0.1753413789289766
1107	20-21	0.15685261651002236
1107	22-23	0.22461157863010328
1107	24-25	0.2039247126633299
1107	26-27	0.21184113136292382
1107	28-29	0.2677395664533293
1107	30-31	0.21248150261553178
1107	32-33	0.2214816604430183
1107	34-35	0.17031740193942824
1107	36-37	0.19891510768764675
1107	38-39	0.1788202211916854
1107	40-41	0.18638720841479994
1107	42-43	0.20218597980294817
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2216	66-67	-1.4043837086791449
2216	68-69	-1.3625113426154698
2216	70-71	-1.462830824393457
2216	72-73	-1.498662039963854
2216	74-75	-1.3971144603897159
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2216	80-81	-1.2614145469499434
2216	82-83	-1.3776541504616198
2216	84-85	-1.3345316703875731
2216	86-87	-1.313131079094326
2216	88-89	-1.2267333357622192
2216	90-91	-1.304772846029472
2216	92-93	-1.2542902744085502
2216	94-95	-1.2273624178866136
2216	96-97	-1.3909704204024393
2216	98-99	-1.6306480152182559
2216	100	-1.663724249564794
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	164432.0
3	33487.0
4	21972.0
5	18588.0
6	13606.0
7	12897.0
8	14581.0
9	15115.0
10	15669.0
11	15880.0
12	16892.0
13	18610.0
14	19693.0
15	20493.0
16	20553.0
17	20439.0
18	19850.0
19	17819.0
20	16573.0
21	18128.0
22	22202.0
23	34731.0
24	53423.0
25	64161.0
26	69131.0
27	57281.0
28	60337.0
29	71694.0
30	82333.0
31	95012.0
32	119287.0
33	154856.0
34	212330.0
35	326653.0
36	541926.0
37	882506.0
38	1015223.0
39	397660.0
40	1052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.31673695811657	21.266736790623042	14.179059791839046	22.23746645942134
2	15.527337862009366	22.095132343187892	38.65961489505664	23.7179148997461
3	18.273306348860082	28.255502793424743	29.165946136289318	24.305244721425858
4	12.549098587409407	19.269989159685082	37.067177712078056	31.11373454082746
5	12.856300641011414	40.44843163863855	33.0414398450418	13.653827875308227
6	28.49316737625238	39.401854413779475	18.04188677222801	14.063091437740132
7	26.044299961499597	35.71217272299617	20.740392746012677	17.503134569491557
8	25.1540862774292	36.82696049810047	19.64116702154853	18.377786202921804
9	25.621298232362776	19.38168704723608	20.505458497914493	34.49155622248665
10-11	23.403802998398398	29.582113152082357	26.84009019877078	20.17399365074846
12-13	23.808970534359137	27.717496205579106	26.82637776731041	21.647155492751345
14-15	21.879235067571095	29.161141157945654	24.732757979106747	24.226865795376504
16-17	21.03070778678933	32.14715018357347	25.576511555561698	21.245630474075508
18-19	20.993365955594324	30.866461948005146	27.320646386770054	20.819525709630476
20-21	23.133808356416154	29.361151418605747	26.540446726017862	20.96459349896024
22-23	22.702570475781854	29.682231862101006	26.577203716169436	21.03799394594771
24-25	21.923849969236773	29.723066056468266	26.817725731853937	21.535358242441028
26-27	21.742876348785035	29.850041837640646	26.895615662287653	21.51146615128667
28-29	21.437809026591363	29.90536793426192	27.222625603816187	21.434197435330532
30-31	21.88127050917712	30.1232931916074	26.66065849178063	21.334777807434854
32-33	21.507470235453514	29.85309904656316	27.149074364054442	21.490356353928885
34-35	21.999014621266113	29.453079214338924	26.998885351801878	21.549020812593085
36-37	21.790159084452657	29.59906388646221	26.998865970338542	21.61191105874659
38-39	21.68381982355407	29.65270287173416	27.018407129851624	21.645070174860145
40-41	21.810588713584053	29.342740843179836	27.131867170265085	21.714803272971025
42-43	21.71283962963378	29.612246758552384	27.200945311225134	21.473968300588705
44-45	21.87490023568714	29.526855741824143	26.88494496464065	21.713299057848072
46-47	21.829429386680175	29.5555488099749	27.016814639119573	21.598207164225347
48-49	21.6341727004629	29.443903358079094	26.862298608149093	22.05962533330891
50-51	21.556230563996067	29.519164645653717	26.9084520384057	22.016152751944514
52-53	21.709747423010068	29.522741495888738	26.859100895039596	21.9084101860616
54-55	21.813889166848448	29.456669780278904	26.87574551893142	21.853695533941234
56-57	21.62779033113653	29.449907493120076	26.80997592183451	22.112326253908883
58-59	21.963250070154427	29.227062014211334	26.94222048890283	21.86746742673141
60-61	21.82276084467424	29.662364974407662	26.554273429617858	21.960600751300234
62-63	21.586026243655436	29.543465818813186	27.013491521534977	21.857016415996398
64-65	22.012227078623777	29.342361446277483	26.768733789679466	21.87667768541927
66-67	21.589154443319593	29.431177887695092	26.73447389994063	22.245193769044683
68-69	21.807828221955685	29.660396467715472	26.90531962251318	21.626455687815664
70-71	21.82977924336654	29.528251288926327	26.728913483463142	21.91305598424399
72-73	21.86317137945757	29.607239687528423	26.83079033855623	21.698798594457777
74-75	21.9319341028466	29.544078146787527	26.62725386590088	21.896733884464993
76-77	21.88258033169425	29.395026271418047	26.3516887677193	22.370704629168404
78-79	21.979281236510676	29.358011826015307	26.824541556641236	21.838165380832777
80-81	21.601397562012103	29.71876053248034	26.788663754032438	21.891178151475124
82-83	21.671312358482243	29.745846064894405	26.93612621209538	21.646715364527974
84-85	21.75488268421898	29.19420991289958	26.841781615180793	22.209125787700646
86-87	22.147160749374052	29.471128529392093	26.518296574180283	21.863414147053568
88-89	21.927740654885707	29.372086234429695	26.600180050184203	22.0999930605004
90-91	21.836569487829724	29.19231579207388	26.563544726925	22.407569993171396
92-93	21.784007022868387	29.378838930279066	26.6916937122531	22.14546033459944
94-95	21.867993441487044	29.15808517798052	26.848663831203595	22.125257549328843
96-97	21.86256679364944	29.549692633031515	26.42398220147849	22.163758371840554
98-99	21.641338314989852	29.533051342769678	26.629288891419538	22.196321450820935
100	21.845970594176823	29.47218358176617	26.41190133024349	22.269944493813515
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	33753.0
1	24869.5
2	14519.5
3	11238.0
4	7855.5
5	5452.0
6	4403.0
7	4483.0
8	5024.5
9	5135.5
10	4050.5
11	2847.0
12	2537.5
13	2488.0
14	2552.0
15	2694.5
16	2926.0
17	3361.5
18	3948.5
19	4818.0
20	6011.0
21	7640.0
22	10608.5
23	15019.5
24	20178.5
25	26271.0
26	34678.0
27	45565.5
28	59294.0
29	72750.0
30	82141.5
31	91295.5
32	100919.5
33	109350.0
34	117809.0
35	125363.5
36	131047.0
37	135569.0
38	139354.0
39	141495.0
40	142328.5
41	142244.0
42	140018.5
43	138151.5
44	137484.5
45	139449.0
46	144017.0
47	142636.0
48	142486.5
49	150199.0
50	155173.0
51	150922.0
52	147437.0
53	146671.0
54	147269.0
55	148657.0
56	134534.0
57	113947.0
58	102249.5
59	95786.0
60	84168.0
61	69459.5
62	58625.0
63	46647.0
64	35358.0
65	27806.5
66	21403.0
67	15838.0
68	12083.5
69	9285.0
70	6922.5
71	5232.0
72	4140.5
73	3064.5
74	2439.0
75	2036.5
76	1455.5
77	1141.0
78	899.0
79	618.5
80	449.0
81	293.0
82	220.5
83	191.0
84	153.0
85	131.5
86	110.5
87	96.5
88	80.0
89	62.5
90	58.5
91	48.5
92	42.0
93	36.5
94	41.5
95	43.5
96	32.0
97	30.0
98	42.5
99	55.0
100	82.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016139583322430564
2	0.008436124616004563
3	0.010487589162824532
4	0.009650256694734748
5	0.011743587864959206
6	0.012120387475599608
7	0.010508522474526777
8	0.0077034587064260035
9	0.006342793445780106
10-11	0.006102060361204293
12-13	0.006803326303229487
14-15	0.007651125427170393
16-17	0.005044928120240943
18-19	0.00576712737396838
20-21	0.005327527828221244
22-23	0.005746194062266135
24-25	0.005610127536201546
26-27	0.005777594029819503
28-29	0.01647451630966648
30-31	0.005233327925561144
32-33	0.005254261237263388
34-35	0.004846061659069619
36-37	0.00636372675748235
38-39	0.0053903277633279775
40-41	0.0052961278606678775
42-43	0.004929794905878597
44-45	0.004008729190979836
46-47	0.005965993835139704
48-49	0.0034016631516147434
50-51	0.0037679961064040236
52-53	0.00283646373565414
54-55	0.004207595652151159
56-57	0.0027841304563985285
58-59	0.0023759308782047594
60-61	0.0032760632814012756
62-63	0.0031190634436344413
64-65	0.004573928606940439
66-67	0.0031609300670389306
68-69	0.0034853963984237215
70-71	0.0048355950032184965
72-73	0.0026899305537384276
74-75	0.0024282641574603706
76-77	0.0024177975016092482
78-79	0.0031190634436344413
80-81	0.0038726626649152464
82-83	0.0014339318516037533
84-85	0.0024596641250137375
86-87	0.0021247311377778242
88-89	0.0015385984101149762
90-91	0.0022817309755446586
92-93	0.002292197631395781
94-95	0.0016223316569239545
96-97	0.001025732273409984
98-99	0.0017374648712862996
100	0.0027213305212917946
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4777075.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.76675492221696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.54787847954478	36.32253611975611
2	15.053806622031694	15.284658228542595
3	5.740854409255092	8.743336465163457
4	2.7598290150343625	5.604302529338916
5	1.5415650533980003	3.913012763125529
6	0.8976335903540393	2.734196669487193
7	0.5708039566582599	2.028450520341114
8	0.38947979907668223	1.5818100405504187
9	0.26464430423094015	1.2091619281007489
>10	1.1183217515245354	9.5856286623784
>50	0.05829387702437519	2.054986879347515
>100	0.04714826527179974	4.970898706737216
>500	0.006549186237002932	2.323304930213677
>1k	0.0031502398345317137	2.8250132528696468
>5k	0.0	0.0
>10k+	4.1450524138524064E-5	0.8187023040474593
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38906	0.8144314250875273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.0933311702244576E-5	0.0
5	0.0	0.0	0.0	2.0933311702244576E-5	0.0
6	0.0	0.0	0.0	2.0933311702244576E-5	0.0
7	0.0	0.0	0.0	2.0933311702244576E-5	0.0
8	0.0	0.0	0.0	2.0933311702244576E-5	0.0
9	0.0	0.0	0.0	8.37332468089783E-5	0.0
10-11	0.0	0.0	0.0	8.37332468089783E-5	0.0
12-13	0.0	0.0	0.0	8.37332468089783E-5	0.0
14-15	0.0	0.0	0.0	9.419990266010058E-5	0.0
16-17	0.0	0.0	0.0	1.2559987021346745E-4	0.0
18-19	0.0	0.0	0.0	1.5699983776683432E-4	0.0
20-21	0.0	0.0	0.0	2.1979977287356803E-4	0.0
22-23	0.0	0.0	0.0	5.547327601094813E-4	0.0
24-25	0.0	0.0	0.0	0.0014025318840503865	0.0
26-27	0.0	0.0	0.0	0.0026794638978873053	0.0
28-29	0.0	0.0	0.0	0.006164860296311027	0.0
30-31	0.0	0.0	0.0	0.0142765185809308	0.0
32-33	0.0	0.0	0.0	0.029495036188462605	0.0
34-35	0.0	0.0	0.0	0.052867078704018675	0.0
36-37	0.0	0.0	0.0	0.08865257505900578	0.0
38-39	0.0	0.0	0.0	0.1363700590842723	0.0
40-41	0.0	0.0	0.0	0.18783460590424056	0.0
42-43	0.0	0.0	0.0	0.23764542109973152	0.0
44-45	0.0	0.0	0.0	0.3019106880256224	0.0
46-47	0.0	0.0	0.0	0.37947907453828966	0.0
48-49	0.0	0.0	0.0	0.4642903868999335	0.0
50-51	0.0	0.0	0.0	0.5483585666961477	0.0
52-53	0.0	0.0	0.0	0.6318824803881036	0.0
54-55	0.0	0.0	0.0	0.7244181847678757	0.0
56-57	0.0	0.0	0.0	0.8364637356541398	2.0933311702244576E-5
58-59	0.0	0.0	0.0	0.9651722026553906	2.0933311702244576E-5
60-61	0.0	0.0	0.0	1.1015736617072163	2.0933311702244576E-5
62-63	0.0	0.0	0.0	1.2419733832941706	2.0933311702244576E-5
64-65	0.0	0.0	0.0	1.3846548358566695	2.0933311702244576E-5
66-67	0.0	0.0	0.0	1.5513572635974944	2.0933311702244576E-5
68-69	0.0	0.0	0.0	1.7439437312581445	2.0933311702244576E-5
70-71	0.0	0.0	0.0	1.9537687811055928	2.0933311702244576E-5
72-73	0.0	0.0	0.0	2.170250624074355	2.0933311702244576E-5
74-75	0.0	0.0	0.0	2.3881873322064235	2.0933311702244576E-5
76-77	0.0	0.0	0.0	2.6275388182098878	2.0933311702244576E-5
78-79	0.0	0.0	0.0	2.9015663350481207	2.0933311702244576E-5
80-81	0.0	0.0	0.0	3.2075590188556804	2.0933311702244576E-5
82-83	0.0	0.0	0.0	3.5257139567622446	2.0933311702244576E-5
84-85	0.0	0.0	0.0	3.84462249389009	2.0933311702244576E-5
86-87	0.0	0.0	0.0	4.182569878011126	2.0933311702244576E-5
88	0.0	0.0	0.0	4.452096732833376	2.0933311702244576E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8955	0.0	44.98823	1
GTATCAA	14305	0.0	34.86673	1
ATCAACG	17220	0.0	28.171339	3
TCAACGC	17175	0.0	28.162745	4
TATCAAC	17960	0.0	27.377314	2
GTACATG	30290	0.0	26.305958	1
TACATGG	30535	0.0	25.47801	2
CATGGGT	11170	0.0	24.197529	4
ACATGGG	31180	0.0	23.985842	3
CAACGCA	20420	0.0	23.687325	5
GTGGTAT	4575	0.0	23.53033	1
TGGTATC	4615	0.0	23.019835	2
AACGCAG	21320	0.0	22.685017	6
ACGCAGA	21845	0.0	21.924438	7
CGCAGAG	21425	0.0	21.914564	8
AATGCGG	1240	0.0	20.089102	7
TAGTACG	235	1.2655073E-7	20.003113	2
ATGCGGG	1275	0.0	19.536821	8
GCAGAGT	22565	0.0	19.41193	9
ATGGGTA	5000	0.0	18.896545	5
>>END_MODULE