FastQCFastQC Report
Wed 25 May 2016
SRR2431454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4777075
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492481.030923734712141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416620.8721236321389134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299500.626952685482225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100540.21046351585436696No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100250.20985644981500184No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98170.20550232098093502No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62750.1313565309315847No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58310.12206214053578812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166900.061.1764071
GTATCAA316300.044.359751
GTGGTAT54850.037.0584871
TGGTATC57150.035.220032
TATCAAC422450.033.0408132
ATCAACG423400.032.977793
TCAACGC428550.032.603434
CAACGCA459000.030.4168995
AACGCAG475400.029.3864526
CGCAGAG526200.026.3439858
ACGCAGA528500.026.2827037
GCAGAGT549950.024.9156889
CATGGGT105550.024.862124
GTACATG288700.024.7077391
TACATGG290050.024.2560962
ACATGGG294450.023.3985143
GAGTACT377400.023.06796612-13
AGTACTT383850.022.1049212-13
CAGAGTA543250.021.63638310-11
AGAGTAC529350.021.45860110-11
Produced by FastQC (version 0.11.5)