##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431454_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4777075
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26088097842299	33.0	31.0	34.0	30.0	34.0
2	32.38578816535223	34.0	31.0	34.0	31.0	34.0
3	32.477754064987465	34.0	31.0	34.0	30.0	34.0
4	36.03583678296866	37.0	35.0	37.0	35.0	37.0
5	35.949862625141954	37.0	35.0	37.0	35.0	37.0
6	35.993528257354136	37.0	35.0	37.0	35.0	37.0
7	35.93315470240681	37.0	35.0	37.0	35.0	37.0
8	35.917419550666466	37.0	35.0	37.0	35.0	37.0
9	37.592405185181306	39.0	37.0	39.0	35.0	39.0
10-11	37.53045859652612	39.0	37.0	39.0	34.5	39.0
12-13	37.5078702972007	39.0	37.0	39.0	35.0	39.0
14-15	38.813897416306006	40.0	38.0	41.0	34.5	41.0
16-17	38.87167921793147	40.0	38.0	41.0	35.0	41.0
18-19	38.97162573332008	40.5	38.5	41.0	35.0	41.0
20-21	39.00688862954841	41.0	39.0	41.0	35.0	41.0
22-23	38.94599163714197	41.0	38.5	41.0	35.0	41.0
24-25	38.87738522003528	41.0	38.0	41.0	35.0	41.0
26-27	38.717918077484654	40.0	38.0	41.0	34.5	41.0
28-29	38.61406299880157	40.0	38.0	41.0	34.0	41.0
30-31	38.39074506889676	40.0	38.0	41.0	34.0	41.0
32-33	38.13973289094268	40.0	37.0	41.0	34.0	41.0
34-35	37.97343698811511	40.0	37.0	41.0	33.0	41.0
36-37	37.80691458685493	40.0	37.0	41.0	33.0	41.0
38-39	37.68435119817043	40.0	37.0	41.0	33.0	41.0
40-41	37.51847071691359	40.0	36.0	41.0	33.0	41.0
42-43	37.32767697806712	40.0	36.0	41.0	32.5	41.0
44-45	37.02408115844947	40.0	36.0	41.0	31.5	41.0
46-47	36.57872987131246	40.0	36.0	41.0	30.5	41.0
48-49	36.2156663858114	40.0	35.0	41.0	30.0	41.0
50-51	36.024531329317625	40.0	35.0	41.0	29.0	41.0
52-53	35.85626633452479	39.5	35.0	41.0	28.5	41.0
54-55	35.685412412407175	39.0	35.0	41.0	28.0	41.0
56-57	35.43435763516378	39.0	35.0	41.0	28.0	41.0
58-59	35.23979809820864	39.0	35.0	41.0	27.5	41.0
60-61	35.01962948038287	38.0	35.0	41.0	27.0	41.0
62-63	34.78592705787537	37.5	35.0	40.5	26.5	41.0
64-65	34.543532496349755	37.0	35.0	40.0	26.0	41.0
66-67	34.282782874457695	37.0	34.0	40.0	26.0	41.0
68-69	33.999600487746164	36.0	34.0	39.5	26.0	41.0
70-71	33.689515655500486	36.0	34.0	39.0	26.0	41.0
72-73	33.384156099705365	35.0	34.0	39.0	25.0	41.0
74-75	33.09357567130514	35.0	34.0	38.5	25.0	40.5
76-77	32.767399088354274	35.0	34.0	37.0	24.0	39.5
78-79	32.47628161584233	35.0	33.0	37.0	24.0	39.0
80-81	32.202262786328454	35.0	33.0	36.5	24.0	39.0
82-83	31.97689129854566	35.0	33.0	36.0	23.5	38.0
84-85	31.775679469131216	35.0	33.0	36.0	23.0	37.0
86-87	31.556050093414903	35.0	33.0	35.0	22.5	37.0
88-89	31.43008169224892	35.0	33.0	35.0	22.5	36.5
90-91	31.255123794372082	35.0	33.0	35.0	20.5	36.0
92-93	31.076728646713732	35.0	33.0	35.0	20.0	36.0
94-95	30.926992040108225	35.0	33.0	35.0	18.5	36.0
96-97	30.7781172579455	35.0	33.0	35.0	16.5	35.5
98-99	30.624530177985484	35.0	33.0	35.0	2.0	35.0
100	30.516155387972766	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12640412380122257
1101	2	0.20647972012944393
1101	3	0.16228398745825245
1101	4	0.14946890757349962
1101	5	0.16420278128050825
1101	6	0.15173120995093115
1101	7	0.15971763404593986
1101	8	0.20879898923386975
1101	9	0.32655036843226526
1101	10-11	0.272284084754034
1101	12-13	0.3371591013490658
1101	14-15	0.46986030115233035
1101	16-17	0.4022326657119777
1101	18-19	0.3948651139416697
1101	20-21	0.38078809680718706
1101	22-23	0.3611719766128587
1101	24-25	0.35717979677819756
1101	26-27	0.3591468487354419
1101	28-29	0.3842652058354332
1101	30-31	0.4559662012114174
1101	32-33	0.37567450551537007
1101	34-35	0.4728134845429608
1101	36-37	0.504884814977963
1101	38-39	0.4342955590906925
1101	40-41	0.4975399016977775
1101	42-43	0.4792790643224123
1101	44-45	0.5083744817505362
1101	46-47	0.4667865087282337
1101	48-49	0.40352205652452966
1101	50-51	0.40572424266301255
1101	52-53	0.4666095957009091
1101	54-55	0.4846015744759171
1101	56-57	0.42553642988866613
1101	58-59	0.474759120021055
1101	60-61	0.50574455574111
1101	62-63	0.5008545813571033
1101	64-65	0.4233147026379811
1101	66-67	0.4240814438606719
1101	68-69	0.38305519535239796
1101	70-71	0.4672339180971221
1101	72-73	0.44631891298811155
1101	74-75	0.44179719383101457
1101	76-77	0.4782518212146414
1101	78-79	0.43009112715353837
1101	80-81	0.4249164661149365
1101	82-83	0.350343017165585
1101	84-85	0.37072344008156577
1101	86-87	0.434677160980641
1101	88-89	0.4562187159621658
1101	90-91	0.4941448653968763
1101	92-93	0.5089437780194856
1101	94-95	0.5087391506536001
1101	96-97	0.5725191573210502
1101	98-99	0.6188773374916643
1101	100	0.5983909867041035
1102	1	0.1329254600269536
1102	2	0.2055340392645988
1102	3	0.22564680035364404
1102	4	0.14392199235059877
1102	5	0.12239466031896029
1102	6	0.10107743697442118
1102	7	0.15704829941648768
1102	8	0.1991615086917662
1102	9	0.24272485166194713
1102	10-11	0.2759826112697965
1102	12-13	0.27082830332117425
1102	14-15	0.356787612747695
1102	16-17	0.3395173976199857
1102	18-19	0.3564889015571282
1102	20-21	0.31864866035808603
1102	22-23	0.28794203644036287
1102	24-25	0.27335585762882886
1102	26-27	0.30821997585588434
1102	28-29	0.27759371439238123
1102	30-31	0.31431099973345056
1102	32-33	0.3302252011296005
1102	34-35	0.3350075752687829
1102	36-37	0.36631996714361037
1102	38-39	0.3446795904389788
1102	40-41	0.3753153313354005
1102	42-43	0.38582422948354633
1102	44-45	0.3173901944435329
1102	46-47	0.253502823811786
1102	48-49	0.2850822330918632
1102	50-51	0.3102088976928741
1102	52-53	0.31423384260984477
1102	54-55	0.3171464745967256
1102	56-57	0.3628809056157323
1102	58-59	0.3545577456251223
1102	60-61	0.2855711258134761
1102	62-63	0.2735316819971061
1102	64-65	0.2941630730995968
1102	66-67	0.26840421028894923
1102	68-69	0.3028438023775877
1102	70-71	0.30011999589414273
1102	72-73	0.2673715990798158
1102	74-75	0.2913799542478799
1102	76-77	0.2344580791018842
1102	78-79	0.2552264561636406
1102	80-81	0.22594993129622765
1102	82-83	0.2058723453446234
1102	84-85	0.21925204104594442
1102	86-87	0.24088755497843906
1102	88-89	0.19430575728774357
1102	90-91	0.21271584400337673
1102	92-93	0.26715773003264687
1102	94-95	0.31663307197037227
1102	96-97	0.25845550347138513
1102	98-99	0.279645005097084
1102	100	0.31376697424336797
1103	1	0.17614866893941183
1103	2	0.22103704340698727
1103	3	0.18542322724782423
1103	4	0.1306280730189613
1103	5	0.13745898001278078
1103	6	0.12967386190603492
1103	7	0.1460649733577455
1103	8	0.14359283840490633
1103	9	0.2902188164923487
1103	10-11	0.2794229086756772
1103	12-13	0.2690564670394977
1103	14-15	0.32807244832011406
1103	16-17	0.33762572833442306
1103	18-19	0.3147602528735618
1103	20-21	0.3623323023765508
1103	22-23	0.3632270520991696
1103	24-25	0.4004017722810431
1103	26-27	0.4022514616380093
1103	28-29	0.41086987382998785
1103	30-31	0.40961690824169494
1103	32-33	0.3766725684286598
1103	34-35	0.40046174489399533
1103	36-37	0.41536019634994403
1103	38-39	0.4176206884472933
1103	40-41	0.45900402160496157
1103	42-43	0.4515831343603338
1103	44-45	0.4572329683289382
1103	46-47	0.34260088787862486
1103	48-49	0.29384790300508
1103	50-51	0.2751952381221727
1103	52-53	0.3288873126020633
1103	54-55	0.24897452911449136
1103	56-57	0.30962756713606865
1103	58-59	0.33619990158798174
1103	60-61	0.29866031616298727
1103	62-63	0.303790084324163
1103	64-65	0.283672720291527
1103	66-67	0.32583674825102094
1103	68-69	0.3515828419489253
1103	70-71	0.33019381629401323
1103	72-73	0.2940013113644042
1103	74-75	0.23467896203634808
1103	76-77	0.29306787222300557
1103	78-79	0.325107687065632
1103	80-81	0.3151303619139014
1103	82-83	0.2774370813888254
1103	84-85	0.2936807074598029
1103	86-87	0.2832071508037757
1103	88-89	0.28252386780238226
1103	90-91	0.3173091140652353
1103	92-93	0.297964410601665
1103	94-95	0.30206292949048574
1103	96-97	0.3134308518265563
1103	98-99	0.3479140076910596
1103	100	0.38915453664456123
1104	1	0.12491850541892546
1104	2	0.19223100822648576
1104	3	0.14279608054371096
1104	4	0.12025634494769122
1104	5	0.10838793472575503
1104	6	0.11138701649747418
1104	7	0.1448368274432852
1104	8	0.13592559534214388
1104	9	0.2387570003348216
1104	10-11	0.2147054528203185
1104	12-13	0.2381963430942875
1104	14-15	0.31598837014857395
1104	16-17	0.27685934674478574
1104	18-19	0.2740078746178014
1104	20-21	0.22285795103901052
1104	22-23	0.2348765026406312
1104	24-25	0.26657870565679787
1104	26-27	0.22894352618728675
1104	28-29	0.22796646774959584
1104	30-31	0.22307474225297597
1104	32-33	0.2284520012717408
1104	34-35	0.23859103121721148
1104	36-37	0.2298745840919807
1104	38-39	0.26322221801801504
1104	40-41	0.3437175243131705
1104	42-43	0.32358793018221377
1104	44-45	0.382533869074166
1104	46-47	0.38045841606420083
1104	48-49	0.2764974160094127
1104	50-51	0.30103389359121735
1104	52-53	0.26681062452382065
1104	54-55	0.33771055357850344
1104	56-57	0.32075750262242053
1104	58-59	0.27579334057980276
1104	60-61	0.28269096447018427
1104	62-63	0.260197154900105
1104	64-65	0.2325881475620193
1104	66-67	0.20303982414561972
1104	68-69	0.2033191907181191
1104	70-71	0.23952751264052097
1104	72-73	0.245666880218117
1104	74-75	0.23881072791019875
1104	76-77	0.295103273151085
1104	78-79	0.34034945685946383
1104	80-81	0.3258673851035212
1104	82-83	0.32825779207003336
1104	84-85	0.28417472159053503
1104	86-87	0.31925500969432363
1104	88-89	0.35758154390887853
1104	90-91	0.31326854440895247
1104	92-93	0.3394827669258582
1104	94-95	0.36962459329218333
1104	96-97	0.34855359902273264
1104	98-99	0.38679783614718133
1104	100	0.43488686193742154
1105	1	0.09756249897237979
1105	2	0.14578673366968076
1105	3	0.11622419740804446
1105	4	0.09720221047933109
1105	5	0.08567075632995369
1105	6	0.1200461346428483
1105	7	0.11669070570272311
1105	8	0.1208473689691516
1105	9	0.21891382499699574
1105	10-11	0.14648155927100248
1105	12-13	0.09109664889205504
1105	14-15	0.17002841312555006
1105	16-17	0.22506995462114787
1105	18-19	0.21708959287062868
1105	20-21	0.21643506166341808
1105	22-23	0.2082960447704707
1105	24-25	0.1908280548034611
1105	26-27	0.23055974999818574
1105	28-29	0.2529676248222046
1105	30-31	0.215584412074044
1105	32-33	0.23409919743686913
1105	34-35	0.2744922461434456
1105	36-37	0.2665762039936297
1105	38-39	0.2710290902028163
1105	40-41	0.25374353778479986
1105	42-43	0.23191081542540815
1105	44-45	0.2669800651979699
1105	46-47	0.17335268855480024
1105	48-49	0.26670971817065947
1105	50-51	0.27597161829058336
1105	52-53	0.25744323330930996
1105	54-55	0.2447565265525924
1105	56-57	0.2709147198628017
1105	58-59	0.24379418772225137
1105	60-61	0.29373923092747845
1105	62-63	0.20097979054620652
1105	64-65	0.1790129378426073
1105	66-67	0.20337917701144193
1105	68-69	0.1711887721057863
1105	70-71	0.21075460880152264
1105	72-73	0.2471875422289429
1105	74-75	0.24140445079131467
1105	76-77	0.24575813492223375
1105	78-79	0.2249753504914409
1105	80-81	0.21303505732139172
1105	82-83	0.21221347205680274
1105	84-85	0.23968494928589124
1105	86-87	0.23793594692313746
1105	88-89	0.2534439555787813
1105	90-91	0.29897386281053784
1105	92-93	0.2837770352840536
1105	94-95	0.2661625494060722
1105	96-97	0.22869046418245276
1105	98-99	0.2955421024391782
1105	100	0.3640748449119222
1106	1	0.08469760152978267
1106	2	0.10826568981408968
1106	3	0.0980772419868643
1106	4	0.10338982725012613
1106	5	0.09083898663712375
1106	6	0.10691625538134275
1106	7	0.0854390031091512
1106	8	0.10052862738167079
1106	9	0.12597441522943598
1106	10-11	0.12880472057039327
1106	12-13	0.13179228141309096
1106	14-15	0.1831058384424722
1106	16-17	0.17899414651699885
1106	18-19	0.15613888270328147
1106	20-21	0.1425170372746436
1106	22-23	0.09906831911404623
1106	24-25	0.13352387817569422
1106	26-27	0.17498482583642527
1106	28-29	0.15803484462250594
1106	30-31	0.14474839343089485
1106	32-33	0.15162537418516564
1106	34-35	0.11330654792941175
1106	36-37	0.11738023999419056
1106	38-39	0.12657129845160142
1106	40-41	0.16454273219185467
1106	42-43	0.187954239094978
1106	44-45	0.22333620357512984
1106	46-47	0.1763105707092052
1106	48-49	0.13199604518916175
1106	50-51	0.14962684058200182
1106	52-53	0.14687257818746957
1106	54-55	0.2023558897122797
1106	56-57	0.21287077386364928
1106	58-59	0.22011342150492652
1106	60-61	0.2824376056788722
1106	62-63	0.16786355212283866
1106	64-65	0.1631809170297771
1106	66-67	0.17779555405304848
1106	68-69	0.16245988125258037
1106	70-71	0.19449682910406807
1106	72-73	0.25154868291640753
1106	74-75	0.24166046260399554
1106	76-77	0.1768260779481352
1106	78-79	0.19319308157205484
1106	80-81	0.20552111513924842
1106	82-83	0.15021293968241878
1106	84-85	0.18663603111082594
1106	86-87	0.11759562910566501
1106	88-89	0.0831692604384564
1106	90-91	0.11335984428792756
1106	92-93	0.22100565492766222
1106	94-95	0.23903644698929227
1106	96-97	0.12578578933262108
1106	98-99	0.22180508534292898
1106	100	0.2663357284525141
1107	1	0.07214089618492636
1107	2	0.11082157687526006
1107	3	0.12649945873208424
1107	4	0.1561255554270602
1107	5	0.10904706295016098
1107	6	0.08776042035713516
1107	7	0.028133214413522012
1107	8	0.012185348063226797
1107	9	0.11203229189595021
1107	10-11	0.08794497390514522
1107	12-13	0.004272607080203272
1107	14-15	0.09471482427538547
1107	16-17	0.14729817761765673
1107	18-19	0.1335059691772429
1107	20-21	0.08353436479930565
1107	22-23	0.11148942456141242
1107	24-25	0.08535767152820029
1107	26-27	0.10525312978912638
1107	28-29	0.17074114398596407
1107	30-31	0.19377430754442315
1107	32-33	0.14690581764592991
1107	34-35	0.17597134022010152
1107	36-37	0.27259536775669346
1107	38-39	0.19026079751989045
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2216	72-73	-0.7363650106515607
2216	74-75	-0.7424722397884835
2216	76-77	-0.6569696922312076
2216	78-79	-0.6321557124947361
2216	80-81	-0.659298564425562
2216	82-83	-0.7064091401304893
2216	84-85	-0.7772984347298362
2216	86-87	-0.7199636179363296
2216	88-89	-0.7615110266963825
2216	90-91	-0.7980365536811185
2216	92-93	-0.6914431895105579
2216	94-95	-0.8474038893298363
2216	96-97	-1.0230404879184896
2216	98-99	-1.0193509185114138
2216	100	-1.0285543111842443
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	317.0
3	1208.0
4	823.0
5	611.0
6	693.0
7	1485.0
8	2943.0
9	5288.0
10	6923.0
11	8317.0
12	10900.0
13	15113.0
14	18299.0
15	25451.0
16	34700.0
17	51411.0
18	57694.0
19	30439.0
20	18631.0
21	17155.0
22	17025.0
23	17669.0
24	19553.0
25	23439.0
26	29027.0
27	36971.0
28	46858.0
29	59482.0
30	75072.0
31	94599.0
32	119354.0
33	151307.0
34	201256.0
35	317231.0
36	507014.0
37	943615.0
38	1226077.0
39	579544.0
40	3581.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.35385997650149	20.787996071014938	15.272253951499234	23.585890000984342
2	14.345996242470548	21.52444749140426	38.962126405802714	25.16742986032248
3	16.714038820464772	27.112760379379154	31.46063652432831	24.712564275827766
4	11.366286748632993	18.50442911849785	37.62080913764004	32.508474995229115
5	12.085615971633006	38.40911702955461	34.187562894888835	15.317704103923552
6	28.547343301078588	38.65361125793503	17.587373026381208	15.211672414605172
7	25.298283991773207	34.926225776233366	21.42417692835051	18.351313303642918
8	26.328035461030026	34.015773250367644	19.540451008200623	20.115740280401713
9	26.441849876755125	15.716709492733525	21.504142179053083	36.33729845145827
10-11	24.05542973695684	27.070588857211796	27.398517428303897	21.47546397752747
12-13	25.5686795790311	25.39370849316789	28.163886897316875	20.873725030484135
14-15	22.73471737412538	26.29807989198411	27.646666631777816	23.320536102112694
16-17	20.054301010555623	29.43512505037078	28.43236708655401	22.078206852519585
18-19	19.859882878121027	26.527236855188583	33.08029495036188	20.532585316328504
20-21	21.62350392237928	25.320850101788228	33.04535725313084	20.010288722701652
22-23	21.119304176719016	25.61371759915848	33.179843314161914	20.087134909960593
24-25	20.688802315154838	25.538536319153753	33.29892922309406	20.473732142597346
26-27	20.45763359377862	25.839797365542722	33.258877032493736	20.443692008184925
28-29	20.178859094507256	25.86264156599362	33.58903123712269	20.36946810237643
30-31	20.709080164215045	25.99583789259452	33.09339076519598	20.201691177994462
32-33	20.286765887231027	25.869343483214337	33.51422185263239	20.329668776922244
34-35	20.678296498647214	25.66825200730208	33.13872011419728	20.51473137985343
36-37	20.617707789372847	25.76959908928127	32.9458843118839	20.66680880946198
38-39	20.63558153833014	25.755236904415753	32.94928565163446	20.659895905619642
40-41	20.619331457966908	25.697976689242797	32.915747509640944	20.76694434314935
42-43	20.704618403570276	25.984598692442134	32.71676745871936	20.59401544526823
44-45	20.987675512735304	25.94142859385712	32.12552660362251	20.945369289785067
46-47	21.035140067497668	26.17841456261537	31.617069851000217	21.169375518886746
48-49	21.164174177314184	26.265406600703383	30.708105198363466	21.86231402361896
50-51	21.23537627606292	26.50701340046615	30.389556369921028	21.868053953549907
52-53	21.343347366810274	26.822672960256998	30.13626983044589	21.69770984248684
54-55	21.448059792006596	26.782413704424705	30.114402756610204	21.65512374695849
56-57	21.235990162824205	26.61428128063323	30.165702714853264	21.9840258416893
58-59	21.528105906526836	26.21695262280273	30.434173214994143	21.820768255676292
60-61	21.307583831734586	26.636225122981887	30.089054389400765	21.967136655882758
62-63	21.15340432905526	26.30755641486642	30.664097805411377	21.874941450666945
64-65	21.26717500843358	26.27225608853022	30.55487027467139	21.90569862836481
66-67	21.19961608266155	26.259694750129263	30.419901738932026	22.12078742827717
68-69	21.502304302778192	26.436132070445694	30.23136809676031	21.830195530015807
70-71	21.392979420063256	26.4705528332254	30.061718981493936	22.074748765217404
72-73	21.43829644709367	26.35625356520465	30.089835307170183	22.115614680531497
74-75	21.520730506968082	26.411724267573444	29.75959511549558	22.307950109962892
76-77	21.509302240387683	26.253146538415244	29.68775872264932	22.549792498547752
78-79	21.550307433296997	26.31252220903999	29.979218356926157	22.157952000736856
80-81	21.061350303271354	26.573583207297354	30.038632426746492	22.326434062684804
82-83	21.203011957333707	26.568619621431864	30.15072242115467	22.077646000079756
84-85	21.20558554153109	26.202039418486063	29.90913007363543	22.68324496634742
86-87	21.332593689653187	26.45453546364669	29.662366615554497	22.550504231145627
88-89	21.184773109067788	26.302434020818175	29.702966773601002	22.809826096513035
90-91	21.04437338748083	26.302245621012858	29.58227576498171	23.071105226524597
92-93	20.9527809794229	26.369315591311636	29.81881316910952	22.859090260155938
94-95	20.859239178786183	26.166576827870614	30.008184924875575	22.965999068467628
96-97	20.812339316551796	26.578841296694165	29.52039179783446	23.088427588919576
98-99	20.62730268115821	26.454467620116223	29.79598244938288	23.12224724934269
100	20.844043388256317	26.397885893248414	29.64526455955167	23.1128061589436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7168.0
1	4158.5
2	2885.0
3	5196.5
4	3662.5
5	2267.0
6	6456.0
7	16846.5
8	27405.5
9	29186.0
10	19043.0
11	8627.0
12	6415.5
13	6522.0
14	7024.5
15	7599.0
16	8180.5
17	8927.5
18	9734.0
19	10526.0
20	11490.0
21	12909.5
22	15359.5
23	19207.5
24	23916.5
25	29156.0
26	36558.5
27	46490.5
28	59281.0
29	72494.0
30	81312.5
31	89475.0
32	98863.0
33	107296.0
34	115923.0
35	123331.5
36	128485.5
37	132684.0
38	136559.5
39	139044.5
40	140975.5
41	141192.0
42	138900.5
43	139107.5
44	140448.0
45	142264.5
46	144433.0
47	143132.0
48	141674.0
49	147687.0
50	155319.5
51	151896.5
52	145159.0
53	143091.0
54	143045.5
55	140526.0
56	125413.5
57	106113.5
58	96967.0
59	94607.5
60	82770.0
61	66139.5
62	56220.0
63	44548.0
64	33119.0
65	25563.5
66	19210.5
67	13842.5
68	10540.5
69	8085.5
70	5714.0
71	4148.5
72	3129.5
73	2196.5
74	1746.0
75	1420.0
76	902.0
77	650.5
78	515.5
79	301.0
80	148.5
81	50.0
82	25.0
83	13.0
84	8.5
85	6.0
86	5.0
87	3.0
88	0.5
89	0.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.5
95	2.5
96	3.0
97	2.0
98	1.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.048251283473673745
2	0.0
3	1.4653318191571203E-4
4	0.06962419472166545
5	0.0053589277957746114
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.012088987508046241
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.17532695216215E-4
26-27	0.0
28-29	3.8726626649152464E-4
30-31	0.003516796365977089
32-33	1.569998377668343E-4
34-35	0.001643264968626199
36-37	0.003945929255873102
38-39	0.0013397319489436529
40-41	0.0011094655202189625
42-43	0.0016327983127750766
44-45	0.0
46-47	4.395995457471361E-4
48-49	4.919328250027475E-4
50-51	0.0016118650010728322
52-53	9.524656824521281E-4
54-55	0.001015265617558862
56-57	0.00622766023141776
58-59	0.008582657797920275
60-61	0.001057132240963351
62-63	0.0025224640601204714
64-65	8.268658122386607E-4
66-67	1.0466655851122287E-4
68-69	7.012659420251933E-4
70-71	4.0819957819376916E-4
72-73	0.0
74-75	1.883998053202012E-4
76-77	0.0
78-79	4.7099951330050295E-4
80-81	0.0
82-83	0.001015265617558862
84-85	5.651994159606035E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	3.5586629893815774E-4
94-95	0.0
96-97	4.0819957819376916E-4
98-99	7.326659095785601E-4
100	4.6053285744938064E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4777075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.82927245101462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.8668639299776	28.868967246808953
2	17.730155345437623	15.541996184679974
3	7.012403795552882	9.220456694754489
4	3.3022749140501495	5.789452276642195
5	1.8150936839277179	3.9777117798491886
6	1.1063546780235511	2.9094432366329257
7	0.7242386946753978	2.2219998547946638
8	0.47765125456664304	1.674808557837627
9	0.3526857165300002	1.391216252343722
>10	1.4597109502878691	10.78018477442445
>50	0.0762401670988662	2.3253090082014007
>100	0.062306389649640234	5.695976499412188
>500	0.008978869928711542	2.7554346354705843
>1k	0.004657487604384384	3.4196492609359566
>5k	1.440460083829921E-4	0.4613645472909109
>10k+	2.4007668063832016E-4	2.9660291899207993
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49248	1.030923734712141	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41662	0.8721236321389134	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29950	0.626952685482225	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10054	0.21046351585436696	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10025	0.20985644981500184	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9817	0.20550232098093502	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6275	0.1313565309315847	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5831	0.12206214053578812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.0933311702244576E-5	0.0
8	0.0	0.0	0.0	2.0933311702244576E-5	0.0
9	0.0	0.0	0.0	6.279993510673372E-5	0.0
10-11	0.0	0.0	0.0	8.37332468089783E-5	0.0
12-13	0.0	0.0	0.0	8.37332468089783E-5	0.0
14-15	0.0	0.0	0.0	8.37332468089783E-5	0.0
16-17	0.0	0.0	0.0	1.0466655851122287E-4	0.0
18-19	0.0	0.0	0.0	1.2559987021346745E-4	0.0
20-21	0.0	0.0	0.0	1.779331494690789E-4	0.0
22-23	0.0	0.0	0.0	5.12866136704992E-4	0.0
24-25	0.0	0.0	0.0	0.0013397319489436526	0.0
26-27	0.0	0.0	0.0	0.002668997242036183	0.0
28-29	0.0	0.0	0.0	0.0062381268872688835	0.0
30-31	0.0	0.0	0.0	0.01454865163305998	0.0
32-33	0.0	0.0	0.0	0.030018368981018718	0.0
34-35	0.0	0.0	0.0	0.054018410847642126	0.0
36-37	0.0	0.0	0.0	0.0904947064888033	0.0
38-39	0.0	0.0	0.0	0.13890298980024388	0.0
40-41	0.0	0.0	0.0	0.19120486908830192	0.0
42-43	0.0	0.0	0.0	0.24211468314816073	0.0
44-45	0.0	0.0	0.0	0.30775108199054857	0.0
46-47	0.0	0.0	0.0	0.38719299990056677	0.0
48-49	0.0	0.0	0.0	0.4735219773606234	0.0
50-51	0.0	0.0	0.0	0.5594950885217418	0.0
52-53	0.0	0.0	0.0	0.6448506669876441	0.0
54-55	0.0	0.0	0.0	0.7394587692259385	0.0
56-57	0.0	0.0	0.0	0.8539953842047696	0.0
58-59	0.0	0.0	0.0	0.9861369143251886	0.0
60-61	0.0	0.0	0.0	1.1256783701323507	0.0
62-63	0.0	0.0	0.0	1.26957395477358	0.0
64-65	0.0	0.0	0.0	1.4151965376302444	0.0
66-67	0.0	1.0466655851122288E-5	0.0	1.5843376961843805	0.0
68-69	0.0	2.0933311702244576E-5	0.0	1.7798966941067493	0.0
70-71	0.0	2.0933311702244576E-5	0.0	1.993154807073366	0.0
72-73	0.0	2.0933311702244576E-5	0.0	2.2136035126097036	0.0
74-75	0.0	2.0933311702244576E-5	0.0	2.435873416264137	0.0
76-77	0.0	2.0933311702244576E-5	0.0	2.6799034974330525	0.0
78-79	0.0	2.0933311702244576E-5	0.0	2.9591748088526977	0.0
80-81	0.0	2.0933311702244576E-5	0.0	3.271102086527844	0.0
82-83	0.0	2.0933311702244576E-5	0.0	3.595589351224337	0.0
84-85	0.0	2.0933311702244576E-5	0.0	3.9220234139091392	0.0
86-87	0.0	2.0933311702244576E-5	0.0	4.269181455179163	0.0
88	0.0	2.0933311702244576E-5	0.0	4.5452290365966626	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16690	0.0	61.176407	1
GTATCAA	31630	0.0	44.35975	1
GTGGTAT	5485	0.0	37.058487	1
TGGTATC	5715	0.0	35.22003	2
TATCAAC	42245	0.0	33.040813	2
ATCAACG	42340	0.0	32.97779	3
TCAACGC	42855	0.0	32.60343	4
CAACGCA	45900	0.0	30.416899	5
AACGCAG	47540	0.0	29.386452	6
CGCAGAG	52620	0.0	26.343985	8
ACGCAGA	52850	0.0	26.282703	7
GCAGAGT	54995	0.0	24.915688	9
CATGGGT	10555	0.0	24.86212	4
GTACATG	28870	0.0	24.707739	1
TACATGG	29005	0.0	24.256096	2
ACATGGG	29445	0.0	23.398514	3
GAGTACT	37740	0.0	23.067966	12-13
AGTACTT	38385	0.0	22.10492	12-13
CAGAGTA	54325	0.0	21.636383	10-11
AGAGTAC	52935	0.0	21.458601	10-11
>>END_MODULE