FastQCFastQC Report
Wed 25 May 2016
SRR2431453_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431453_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4187303
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA574831.3727929409455202No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55880.13345105429437518No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43770.10453029073845385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78100.041.230051
TACCTGG43550.039.071822
GTATCAA122550.032.643041
GTACCTG54900.032.1094781
GTCTCGC12450.027.185511
ATCAACG138850.027.1501183
TCAACGC139000.026.9517354
ACCTGGG61000.026.738863
TATCAAC148200.026.4204392
GTACACG5250.025.9664761
CCGGTCG2601.8189894E-1225.3079199
CAACGCA151300.024.7917525
TCTGTCG13100.024.7559458
GTACATG226050.024.3307611
CATGGGT105500.023.7475034
TACATGG225750.023.5909462
AACGCAG161000.023.238036
ACATGGG232550.021.6883543
CGCAGAG169650.021.4431618
ACGCAGA175750.021.0738077
Produced by FastQC (version 0.11.5)