##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431453_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4187303 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.61800041697484 31.0 30.0 34.0 24.0 34.0 2 29.64103027652883 31.0 30.0 34.0 23.0 34.0 3 29.744555385650383 31.0 30.0 34.0 23.0 34.0 4 32.928594372081506 37.0 35.0 37.0 23.0 37.0 5 33.15810319912364 37.0 35.0 37.0 26.0 37.0 6 33.129187928363436 37.0 35.0 37.0 25.0 37.0 7 33.006118974432944 37.0 35.0 37.0 25.0 37.0 8 32.90466847037341 37.0 35.0 37.0 23.0 37.0 9 34.13995834550306 39.0 35.0 39.0 18.0 39.0 10-11 34.11860725149339 39.0 35.0 39.0 18.0 39.0 12-13 34.087382618358404 39.0 35.0 39.0 17.5 39.0 14-15 35.248798689753286 40.0 36.0 41.0 18.0 41.0 16-17 35.2741675250155 40.0 36.0 41.0 19.0 41.0 18-19 35.27213602645904 40.0 35.5 41.0 20.5 41.0 20-21 35.38389268701118 40.0 35.5 41.0 22.5 41.0 22-23 35.364940392419655 40.0 35.0 41.0 24.0 41.0 24-25 35.42253975888538 40.0 35.0 41.0 25.0 41.0 26-27 35.435398513076315 40.0 35.0 41.0 25.0 41.0 28-29 35.33382060003778 40.0 35.0 41.0 25.0 41.0 30-31 35.307711550847884 40.0 35.0 41.0 25.0 41.0 32-33 35.23815603981847 40.0 35.0 41.0 25.0 41.0 34-35 35.12429002630094 40.0 35.0 41.0 25.0 41.0 36-37 34.98950087920554 40.0 35.0 41.0 24.5 41.0 38-39 34.87276416347229 40.0 34.0 41.0 24.0 41.0 40-41 34.78062000767559 40.0 34.0 41.0 24.0 41.0 42-43 34.56132252669559 39.0 34.0 41.0 23.0 41.0 44-45 34.45207667083084 39.0 34.0 41.0 22.0 41.0 46-47 34.317931016695 39.0 33.0 41.0 22.0 41.0 48-49 34.18853018279308 39.0 33.0 41.0 21.0 41.0 50-51 34.035130130301056 39.0 33.0 41.0 20.0 41.0 52-53 33.93960312879197 39.0 33.0 41.0 19.0 41.0 54-55 33.87375883713216 38.5 33.0 41.0 18.5 41.0 56-57 33.71991327114374 38.0 33.0 40.5 17.5 41.0 58-59 33.514196846991965 38.0 33.0 40.0 15.0 41.0 60-61 33.29117357879284 38.0 32.5 40.0 13.0 41.0 62-63 32.99808337251926 37.0 32.0 40.0 8.5 41.0 64-65 32.83790126962391 37.0 32.0 40.0 7.5 41.0 66-67 32.569334246888744 36.5 32.0 40.0 3.5 41.0 68-69 32.288339535018125 36.0 32.0 39.5 2.0 41.0 70-71 31.973739182476166 35.5 31.0 39.0 2.0 41.0 72-73 31.65190147452907 35.0 31.0 39.0 2.0 41.0 74-75 31.31630646265627 35.0 31.0 38.0 2.0 40.0 76-77 30.995608987455647 35.0 31.0 37.0 2.0 39.5 78-79 30.663180811133085 35.0 30.5 37.0 2.0 39.0 80-81 30.37115119206802 35.0 30.0 36.0 2.0 39.0 82-83 30.0778774547722 35.0 30.0 36.0 2.0 38.0 84-85 29.794552245204134 35.0 30.0 35.5 2.0 37.0 86-87 29.539901698061975 35.0 30.0 35.0 2.0 37.0 88-89 29.278458831376664 35.0 30.0 35.0 2.0 36.5 90-91 29.05760975501415 34.0 29.0 35.0 2.0 36.0 92-93 28.86198574595629 34.0 29.0 35.0 2.0 36.0 94-95 28.63453325445997 34.0 29.0 35.0 2.0 36.0 96-97 28.533684808574876 34.0 29.0 35.0 2.0 35.0 98-99 28.377394232039094 34.0 29.0 35.0 2.0 35.0 100 28.244819875705197 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4440146972675372 1101 2 0.48668955745030473 1101 3 0.5342716307587558 1101 4 0.40517919882694287 1101 5 0.3968622042940311 1101 6 0.44968963466739353 1101 7 0.4495513289466544 1101 8 0.5216538756268392 1101 9 0.6354624763970236 1101 10-11 0.6641329361323258 1101 12-13 0.639828334991563 1101 14-15 0.7900372169486758 1101 16-17 0.7005994925929286 1101 18-19 0.7487409036754187 1101 20-21 0.6841639390077887 1101 22-23 0.6596048625682371 1101 24-25 0.7355712591452317 1101 26-27 0.7435006134361686 1101 28-29 0.7978151502138289 1101 30-31 0.7790796443419907 1101 32-33 0.7212067267314666 1101 34-35 0.7449899815401864 1101 36-37 0.7339916547268004 1101 38-39 0.7404948569458298 1101 40-41 0.7095487260059556 1101 42-43 0.7132067142268852 1101 44-45 0.6195721775996574 1101 46-47 0.5549519629057116 1101 48-49 0.5519697936571859 1101 50-51 0.5893991582152438 1101 52-53 0.549341601713941 1101 54-55 0.6891953995789422 1101 56-57 0.751818204704712 1101 58-59 0.6979094434081858 1101 60-61 0.6747591668297019 1101 62-63 0.7091795110370427 1101 64-65 0.6709899087215021 1101 66-67 0.6457515615681473 1101 68-69 0.58961949301451 1101 70-71 0.6791180974844657 1101 72-73 0.6628879838516362 1101 74-75 0.6336107514017044 1101 76-77 0.5495557205143058 1101 78-79 0.5483095498850368 1101 80-81 0.5485798092621366 1101 82-83 0.5625929068066 1101 84-85 0.585543585860286 1101 86-87 0.6104938668302573 1101 88-89 0.5639312796402223 1101 90-91 0.5171783068293081 1101 92-93 0.550543633676476 1101 94-95 0.5701213738309221 1101 96-97 0.597023171061732 1101 98-99 0.608162790576575 1101 100 0.6042320441676949 1102 1 0.2804331000412219 1102 2 0.31313232490815324 1102 3 0.3088731820775408 1102 4 0.31503065288684695 1102 5 0.25187084973796914 1102 6 0.19421742807437425 1102 7 0.33350642238397654 1102 8 0.39796687778863316 1102 9 0.47363518995804554 1102 10-11 0.43215013468499563 1102 12-13 0.43151672908707894 1102 14-15 0.5044367647796122 1102 16-17 0.432124050747575 1102 18-19 0.39942898280140327 1102 20-21 0.4084221758596982 1102 22-23 0.4135811311395443 1102 24-25 0.4559548499742334 1102 26-27 0.510143227566374 1102 28-29 0.4780937692340146 1102 30-31 0.5310808296305112 1102 32-33 0.5643192139688225 1102 34-35 0.6013557712812769 1102 36-37 0.5637837844331557 1102 38-39 0.5071992606109319 1102 40-41 0.6063030532297731 1102 42-43 0.6052369784152489 1102 44-45 0.5230449240074577 1102 46-47 0.46841992295185264 1102 48-49 0.5083915168910877 1102 50-51 0.43979245413216717 1102 52-53 0.42104623621705883 1102 54-55 0.4586151245730363 1102 56-57 0.5119042626321644 1102 58-59 0.4538350205316064 1102 60-61 0.48846572640846375 1102 62-63 0.5118230383956188 1102 64-65 0.45874137271020743 1102 66-67 0.5005483073076746 1102 68-69 0.453658326980829 1102 70-71 0.4682924807883957 1102 72-73 0.4749279423642143 1102 74-75 0.45493724936955715 1102 76-77 0.44984051674251546 1102 78-79 0.45373772852532923 1102 80-81 0.44632076175712854 1102 82-83 0.47774989717101235 1102 84-85 0.45435743595544054 1102 86-87 0.48669361726918936 1102 88-89 0.5010574024082928 1102 90-91 0.4465680976446791 1102 92-93 0.4528836883090115 1102 94-95 0.4210154588007846 1102 96-97 0.4637705626720816 1102 98-99 0.5235216733007668 1102 100 0.5259977656552053 1103 1 0.25621978018924807 1103 2 0.34678554570932363 1103 3 0.4159843957050562 1103 4 0.3207660997818138 1103 5 0.2996891668563606 1103 6 0.32762373063316375 1103 7 0.3952676791443608 1103 8 0.390213479409546 1103 9 0.5002032968554389 1103 10-11 0.5533317530402329 1103 12-13 0.4871519928473802 1103 14-15 0.5907164524357142 1103 16-17 0.5013677717029168 1103 18-19 0.43796256276380063 1103 20-21 0.4781827881502636 1103 22-23 0.452592875278107 1103 24-25 0.5419803304036392 1103 26-27 0.4708377977464977 1103 28-29 0.5905688644954523 1103 30-31 0.53227358927343 1103 32-33 0.6702724968808766 1103 34-35 0.6365008665801426 1103 36-37 0.6468583177141838 1103 38-39 0.6403924503918148 1103 40-41 0.5658136666827147 1103 42-43 0.5821325849636452 1103 44-45 0.5103263834736183 1103 46-47 0.506983894945364 1103 48-49 0.5195225274113824 1103 50-51 0.48057290391381 1103 52-53 0.48255926776433 1103 54-55 0.5696716462663076 1103 56-57 0.5726870916108808 1103 58-59 0.5813997592434887 1103 60-61 0.6404633192008404 1103 62-63 0.5659402598926349 1103 64-65 0.651360197330149 1103 66-67 0.5787221539215892 1103 68-69 0.4750324867914273 1103 70-71 0.5210940278561829 1103 72-73 0.48277905169036117 1103 74-75 0.48212314801145695 1103 76-77 0.5046257392070785 1103 78-79 0.4815370528923353 1103 80-81 0.41858071886127135 1103 82-83 0.43229283107480754 1103 84-85 0.42942085947485964 1103 86-87 0.3908411344046847 1103 88-89 0.4019053570869673 1103 90-91 0.4320605239618658 1103 92-93 0.33940242403227217 1103 94-95 0.34640061258843247 1103 96-97 0.34049794706017167 1103 98-99 0.36228613630703066 1103 100 0.4310975144559954 1104 1 0.20360591661016159 1104 2 0.31899686793163085 1104 3 0.25840516796568735 1104 4 0.28675537505390025 1104 5 0.2504265082504631 1104 6 0.28558450196948115 1104 7 0.2840105080344202 1104 8 0.2868171702367164 1104 9 0.4134365725495712 1104 10-11 0.43356571499836605 1104 12-13 0.37094262477705797 1104 14-15 0.4384772981536287 1104 16-17 0.498608436724993 1104 18-19 0.4205709589029354 1104 20-21 0.4336244060067287 1104 22-23 0.4201061340379937 1104 24-25 0.4366316132464263 1104 26-27 0.5215578953407416 1104 28-29 0.520459956056321 1104 30-31 0.4639744623198183 1104 32-33 0.5644659797273235 1104 34-35 0.47851577734402184 1104 36-37 0.48012439804805496 1104 38-39 0.4520551123985115 1104 40-41 0.4641486044960388 1104 42-43 0.3989390659291985 1104 44-45 0.3898412179375015 1104 46-47 0.3463192587306878 1104 48-49 0.32350139797955535 1104 50-51 0.30539644381521924 1104 52-53 0.32475152152679243 1104 54-55 0.36482723940506645 1104 56-57 0.4346819202149206 1104 58-59 0.4712089896250191 1104 60-61 0.4626034971237232 1104 62-63 0.49155266548773113 1104 64-65 0.4573548048822005 1104 66-67 0.5377212592496008 1104 68-69 0.5065567580392312 1104 70-71 0.4488100244344544 1104 72-73 0.48323974018667215 1104 74-75 0.540526873544227 1104 76-77 0.42575105083807685 1104 78-79 0.5584267967772156 1104 80-81 0.5268279094952923 1104 82-83 0.4589699813613919 1104 84-85 0.40630784565976086 1104 86-87 0.4329190035680597 1104 88-89 0.44585595523536625 1104 90-91 0.4392647699042307 1104 92-93 0.42139941737629627 1104 94-95 0.3820873213029401 1104 96-97 0.3872263556863409 1104 98-99 0.37598078719747363 1104 100 0.3348364542260853 1105 1 0.09125785435814038 1105 2 0.21218651536591082 1105 3 0.22211006530289978 1105 4 0.14156203840725112 1105 5 0.16635724602257795 1105 6 0.2636158264805175 1105 7 0.19217751978519004 1105 8 0.2159473216323704 1105 9 0.31695510315012143 1105 10-11 0.30842259624402146 1105 12-13 0.2071927556361004 1105 14-15 0.27665789312681 1105 16-17 0.2554660904387944 1105 18-19 0.2380251998127889 1105 20-21 0.2653467472014128 1105 22-23 0.2567800110115215 1105 24-25 0.23730527348964614 1105 26-27 0.30444413314792484 1105 28-29 0.3794009124541944 1105 30-31 0.38452528402935116 1105 32-33 0.4136542058884345 1105 34-35 0.31888475668183247 1105 36-37 0.24408687183093747 1105 38-39 0.2611830799672106 1105 40-41 0.2457899756557893 1105 42-43 0.2743741389469463 1105 44-45 0.23178719537835946 1105 46-47 0.20940043814009357 1105 48-49 0.2367339074818915 1105 50-51 0.23691320038285113 1105 52-53 0.2714791098760969 1105 54-55 0.3364508014746903 1105 56-57 0.3521592600119945 1105 58-59 0.35205829575757974 1105 60-61 0.3720244770218528 1105 62-63 0.3920239392883502 1105 64-65 0.4023323866752335 1105 66-67 0.43285588255997 1105 68-69 0.3879552990488051 1105 70-71 0.3710979000532255 1105 72-73 0.36202312576768136 1105 74-75 0.34607411382020103 1105 76-77 0.25696622660709423 1105 78-79 0.2698479372242062 1105 80-81 0.26171826027429645 1105 82-83 0.2629570273284685 1105 84-85 0.2839998907729253 1105 86-87 0.23460346333394 1105 88-89 0.31247246104401327 1105 90-91 0.2871923783594639 1105 92-93 0.31059693106506003 1105 94-95 0.30146047821588695 1105 96-97 0.35844338853447155 1105 98-99 0.34181828723211893 1105 100 0.2995275650429612 1106 1 0.2419537349525136 1106 2 0.25613022295286214 1106 3 0.30808429749079735 1106 4 0.272807710630552 1106 5 0.2467437614003103 1106 6 0.15006251640182455 1106 7 0.24130441922352475 1106 8 0.20478959145495423 1106 9 0.3127143168451312 1106 10-11 0.3693728057360488 1106 12-13 0.2740247729748546 1106 14-15 0.3110948518752892 1106 16-17 0.3691798138101632 1106 18-19 0.34585162649704415 1106 20-21 0.3777184362162913 1106 22-23 0.4416569879314025 1106 24-25 0.5008710262283174 1106 26-27 0.4653853466109652 1106 28-29 0.5674843771524891 1106 30-31 0.5548180641865272 1106 32-33 0.5080473142138473 1106 34-35 0.42498230172453333 1106 36-37 0.43211958408266327 1106 38-39 0.4391058636308145 1106 40-41 0.493129582398673 1106 42-43 0.42823657793867653 1106 44-45 0.430226182060089 1106 46-47 0.446974392855509 1106 48-49 0.37038254506023094 1106 50-51 0.4545165763134591 1106 52-53 0.3820905947823974 1106 54-55 0.46676210814312924 1106 56-57 0.42229787848530265 1106 58-59 0.4628978381161062 1106 60-61 0.5466789912413077 1106 62-63 0.547405137835149 1106 64-65 0.5910156295882985 1106 66-67 0.5046537929403954 1106 68-69 0.4828238105276057 1106 70-71 0.5201226220222033 1106 72-73 0.42192192598190914 1106 74-75 0.4397457875204438 1106 76-77 0.48333895272934413 1106 78-79 0.429166399510887 1106 80-81 0.3763154421387256 1106 82-83 0.41531016406288046 1106 84-85 0.45615204781890384 1106 86-87 0.4636030634216688 1106 88-89 0.47229514933836114 1106 90-91 0.5172253492824481 1106 92-93 0.43214136138540127 1106 94-95 0.4566412696659583 1106 96-97 0.5233845235560501 1106 98-99 0.5498584282298609 1106 100 0.583595412160772 1107 1 0.13633569394957945 1107 2 0.06819577578595926 1107 3 0.14470318227436252 1107 4 0.2577289115241328 1107 5 0.12836015690036362 1107 6 0.22736342380508745 1107 7 0.20216825348664003 1107 8 0.25563192243969723 1107 9 0.30468893150391807 1107 10-11 0.3061045264251234 1107 12-13 0.25288644434239416 1107 14-15 0.32977340007487044 1107 16-17 0.2843076395451334 1107 18-19 0.3282185524863692 1107 20-21 0.27377266364932495 1107 22-23 0.3086004725475604 1107 24-25 0.31632944573673427 1107 26-27 0.36083097191139757 1107 28-29 0.4079388116919205 1107 30-31 0.3172065643810811 1107 32-33 0.35649201908287154 1107 34-35 0.31222943040039297 1107 36-37 0.3176631991793286 1107 38-39 0.3904142711127676 1107 40-41 0.3599290232960115 1107 42-43 0.4288396552430953 1107 44-45 0.36824360741797335 1107 46-47 0.374142063551858 1107 48-49 0.40114659271361575 1107 50-51 0.3624407602409576 1107 52-53 0.3277055423792774 1107 54-55 0.35156532160220877 1107 56-57 0.3659187612906365 1107 58-59 0.3437808624468772 1107 60-61 0.32425427355816794 1107 62-63 0.38835164293957547 1107 64-65 0.4914798271461933 1107 66-67 0.45994654724021444 1107 68-69 0.5077815997203174 1107 70-71 0.44425105237744944 1107 72-73 0.42562657215087896 1107 74-75 0.410135047752167 1107 76-77 0.3871887749580445 1107 78-79 0.34168042156217027 1107 80-81 0.32588462375115057 1107 82-83 0.374206960396684 1107 84-85 0.4085682206833141 1107 86-87 0.3832344702781043 1107 88-89 0.3977528608226244 1107 90-91 0.33631284675801254 1107 92-93 0.2908367379130219 1107 94-95 0.3697084091450975 1107 96-97 0.41339620657674914 1107 98-99 0.4059386323100469 1107 100 0.4517132089638132 1108 1 0.14926725060032098 1108 2 0.20819513179623783 1108 3 0.20607953412118363 1108 4 0.18386596347284012 1108 5 0.14607813678966153 1108 6 0.1886224601655968 1108 7 0.11149462201036897 1108 8 0.21366858003582934 1108 9 0.24739097872294735 1108 10-11 0.31763030211378407 1108 12-13 0.23635771905929914 1108 14-15 0.3681033646129066 1108 16-17 0.3043094135545559 1108 18-19 0.32209159781049124 1108 20-21 0.3017428991733766 1108 22-23 0.32187735349877045 1108 24-25 0.31192972010065745 1108 26-27 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-0.29606578437721964 2215 62-63 -0.24846252449674466 2215 64-65 -0.2659642889831346 2215 66-67 -0.24156927159557995 2215 68-69 -0.32478837357988155 2215 70-71 -0.2939923266314928 2215 72-73 -0.2794483067030349 2215 74-75 -0.19045164661129377 2215 76-77 -0.18206301717137663 2215 78-79 -0.19111026057000302 2215 80-81 -0.19193576560355297 2215 82-83 -0.23688638371736204 2215 84-85 -0.2698041215254854 2215 86-87 -0.25253925961898815 2215 88-89 -0.26997807270616647 2215 90-91 -0.2519569362362901 2215 92-93 -0.28938574579265364 2215 94-95 -0.3290692287609467 2215 96-97 -0.2605638762945155 2215 98-99 -0.21473207274209472 2215 100 -0.31378872422424564 2216 1 -0.8003194941482192 2216 2 -0.8389626136927077 2216 3 -0.9100324825041 2216 4 -0.729472509910579 2216 5 -0.8997565016345135 2216 6 -0.9826678855917663 2216 7 -1.0100953248504538 2216 8 -0.9135821942818936 2216 9 -1.2686975299874632 2216 10-11 -1.4578517458054634 2216 12-13 -1.431635864126477 2216 14-15 -1.6654570925129306 2216 16-17 -1.5583859783514669 2216 18-19 -1.5241913406745269 2216 20-21 -1.4340714525497447 2216 22-23 -1.2452804397440147 2216 24-25 -1.4215383007781952 2216 26-27 -1.5070793215185674 2216 28-29 -4.741299861332898 2216 30-31 -2.354137819362194 2216 32-33 -1.927335589407022 2216 34-35 -1.6040280858060427 2216 36-37 -1.6549508014410819 2216 38-39 -1.5959798326405874 2216 40-41 -1.5806398945267972 2216 42-43 -1.7689509388858369 2216 44-45 -1.5493475841491886 2216 46-47 -1.5176951593326393 2216 48-49 -1.5674895976508694 2216 50-51 -1.4610900560676257 2216 52-53 -1.5927557911439578 2216 54-55 -1.8429393512166925 2216 56-57 -1.676808467742866 2216 58-59 -1.792522384307368 2216 60-61 -1.8314722418036382 2216 62-63 -1.8390096634434059 2216 64-65 -1.7951228773101633 2216 66-67 -1.8986070075701917 2216 68-69 -1.9069131364162715 2216 70-71 -1.9462573345327066 2216 72-73 -1.9812128587725617 2216 74-75 -1.889228357976478 2216 76-77 -1.8154170968160734 2216 78-79 -1.8120537988964642 2216 80-81 -1.735871579647707 2216 82-83 -1.8159167559257625 2216 84-85 -1.7579749075092757 2216 86-87 -1.8578052018870892 2216 88-89 -1.7899536977475101 2216 90-91 -1.9242283950660166 2216 92-93 -1.9188927547186552 2216 94-95 -1.8002508407963091 2216 96-97 -1.8606371342600205 2216 98-99 -2.1532270883429305 2216 100 -2.1497261502176492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 151433.0 3 31474.0 4 22138.0 5 19318.0 6 13557.0 7 12631.0 8 13945.0 9 14037.0 10 13927.0 11 14164.0 12 15732.0 13 17318.0 14 18446.0 15 18851.0 16 18664.0 17 18046.0 18 16840.0 19 15467.0 20 14770.0 21 15953.0 22 20310.0 23 32577.0 24 51215.0 25 60819.0 26 63994.0 27 49807.0 28 51178.0 29 60832.0 30 69664.0 31 80117.0 32 100688.0 33 130678.0 34 177565.0 35 275417.0 36 421530.0 37 730231.0 38 942224.0 39 390654.0 40 1092.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54485437078722 21.870034824896216 13.234248780650926 21.35086202366564 2 16.043015498610448 23.489236571867487 38.06361948871686 22.404128440805206 3 17.461733834775387 29.488580697028656 29.462976771602957 23.586708696593007 4 12.253935362324656 20.77170073954336 37.35732908389676 29.617034814235232 5 11.869173543987586 40.522385796028445 32.71050716425247 14.897933495731493 6 27.032063547643652 40.72286232864557 18.44177669520056 13.803297428510215 7 24.769208905618736 36.448070660254444 21.470862557158878 17.31185787696794 8 23.755027835357602 37.51383169717462 20.66609011208899 18.065050355378784 9 24.280557498079766 20.919170612219943 20.961229445864717 33.839042443835574 10-11 21.953852798933465 30.824158836803644 27.506974241431454 19.715014122831434 12-13 22.869101347067645 29.169842680297165 27.089275608561632 20.871780364073565 14-15 21.117529480353316 30.860537561909755 25.060861855946857 22.961071101790065 16-17 19.956601986406685 33.55978111600078 25.504972703984517 20.978644193608016 18-19 19.908660836602156 32.080409225437016 27.581972146379584 20.428957791581244 20-21 22.2178781416947 31.20524072846966 26.58156939316366 19.995311736671976 22-23 21.50149145329612 31.533367319290186 26.708424254127067 20.256716973286633 24-25 20.96563828801417 31.293962996328673 27.07819645554238 20.662202260114775 26-27 20.80103052144772 31.0748980208175 27.119924461545498 21.00414699618928 28-29 20.742723095080052 31.1984497083171 27.269693761021855 20.78913343558099 30-31 21.262908126678916 31.89221270144738 26.522479769431527 20.322399402442176 32-33 20.438265290647145 31.35675617194241 27.463349520533892 20.741629016876548 34-35 20.720823783597623 30.656075650027866 27.413796830027103 21.209303736347408 36-37 21.403375945640946 31.24078839735131 26.715026958603765 20.64080869840398 38-39 21.07655823216871 31.185708432603214 27.104436953319045 20.63329638190903 40-41 20.678273992877305 30.93183142886519 27.39025987216027 20.999634706097233 42-43 20.551864635894606 31.051407574853823 27.338949422359633 21.057778366891945 44-45 21.103400263069748 31.128483241095722 26.999315158871795 20.768801336962735 46-47 20.781153160425745 31.703731656084344 26.83051051883024 20.684604664659666 48-49 20.594514335827004 30.957490712019585 27.292738800687438 21.155256151465966 50-51 20.62413529487206 30.736978940899967 27.288201129129057 21.35068463509891 52-53 20.650689172292296 30.934352767227335 27.091880046155385 21.32307801432498 54-55 20.88418420474412 31.411425145925083 26.842274329604404 20.862116319726397 56-57 20.50360584199781 31.124483197276632 26.835219116712306 21.536691844013255 58-59 20.474514954094413 30.52029515800726 27.350417858697774 21.654772029200554 60-61 21.04440399514274 31.190662731278394 26.605772993439786 21.159160280139076 62-63 20.791075074931637 31.101787612098917 26.85955841085225 21.24757890211719 64-65 20.722008356888903 30.710283014350438 27.26492622730685 21.302782401453804 66-67 20.57866260026199 30.815947821372745 26.800811051895618 21.80457852646964 68-69 21.285417850600286 31.143001905956492 26.67248846556591 20.899091777877317 70-71 21.297274175890312 30.752419834041227 26.861411719524707 21.08889427054375 72-73 20.90441092969884 30.872006053718515 27.311171472627688 20.912411543954956 74-75 20.91528687930466 30.902106167422026 27.023707369567912 21.158899583705402 76-77 20.96943469743676 30.964795539721518 26.655955406768157 21.409814356073564 78-79 21.246496269713166 31.05222425450089 26.827865625368315 20.87341385041763 80-81 20.839945380085464 30.878928045367726 27.054292123399932 21.226834451146882 82-83 20.748600893025916 30.896910351952705 27.146860647190042 21.207628107831333 84-85 20.912350748420746 30.654507976543183 26.970635511913915 21.462505763122156 86-87 21.33589495855868 30.758441439960087 26.7346287858515 21.171034815629735 88-89 20.97518028389033 30.64737174382355 26.929292270722634 21.448155701563483 90-91 20.817254043132717 30.44504163589326 26.854342139751157 21.88336218122286 92-93 21.077237956720378 30.771519802062958 26.6324074140414 21.518834827175265 94-95 21.00744445852171 30.506959469536802 26.911879358288466 21.57371671365302 96-97 20.996648146091633 30.567415012532134 26.85211123808432 21.58382560329191 98-99 20.85757676279973 30.622117267865505 26.81089850370783 21.70940746562693 100 21.062615695491257 30.91569809444406 26.534918394899478 21.486767815165205 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 48565.0 1 36075.5 2 20014.0 3 13756.0 4 9187.0 5 6277.5 6 4902.5 7 4544.0 8 4599.0 9 4614.5 10 3960.0 11 3187.5 12 2966.5 13 2849.5 14 2912.0 15 3109.0 16 3335.5 17 3754.5 18 4420.5 19 5273.0 20 6364.0 21 8164.0 22 10998.5 23 15260.0 24 20398.0 25 26551.5 26 35085.5 27 45383.0 28 58829.0 29 71814.5 30 80567.5 31 89398.5 32 98965.0 33 107476.0 34 115357.0 35 121896.0 36 127230.0 37 131517.5 38 133716.5 39 133453.5 40 133719.5 41 133072.0 42 129249.5 43 126516.5 44 124185.0 45 122194.0 46 122177.0 47 121612.5 48 122123.5 49 124356.5 50 125502.0 51 121839.5 52 114857.0 53 110120.5 54 107661.0 55 105807.0 56 98249.5 57 87767.5 58 81142.0 59 74997.5 60 64854.0 61 53171.5 62 43359.0 63 33703.5 64 25455.5 65 19436.0 66 14498.0 67 10686.5 68 7840.5 69 5941.0 70 4433.0 71 3226.0 72 2561.0 73 2028.0 74 1590.0 75 1194.0 76 789.5 77 623.0 78 528.0 79 374.0 80 260.0 81 181.0 82 147.0 83 125.5 84 118.0 85 96.5 86 66.0 87 59.5 88 46.5 89 31.5 90 34.0 91 34.0 92 23.0 93 19.5 94 23.5 95 23.0 96 17.0 97 15.0 98 19.5 99 22.5 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015355946297652691 2 0.008286957022216926 3 0.010627365633678767 4 0.009433279607422726 5 0.011319935528907272 6 0.013994688227720802 7 0.012131914026761378 8 0.008645182830093738 9 0.007427215083312576 10-11 0.006889876371497358 12-13 0.007630209707776103 14-15 0.008645182830093738 16-17 0.0058510215286546015 18-19 0.006471946262307743 20-21 0.006006252712067887 22-23 0.00667494088677127 24-25 0.006185365616006294 26-27 0.007080930135698324 28-29 0.017576946306488928 30-31 0.006221188196793974 32-33 0.005970430131280206 34-35 0.005552500022090592 36-37 0.00783320433223963 38-39 0.0065555322841456655 40-41 0.006292833358369337 42-43 0.005385327978414746 44-45 0.004788284965286725 46-47 0.0068779355112347975 48-49 0.004083774209795661 50-51 0.004346473135571989 52-53 0.0034031451748297178 54-55 0.005325623677101944 56-57 0.003331500013254355 58-59 0.0029135699040647406 60-61 0.004024069908482859 62-63 0.003653903240343486 64-65 0.0052181559347388995 66-67 0.003534494637717882 68-69 0.003976306467432617 70-71 0.005707731205503877 72-73 0.0029732742053775424 74-75 0.002961333345114982 76-77 0.002877747323277059 78-79 0.0037852527032316507 80-81 0.0044300591574099124 82-83 0.0018747150612219848 84-85 0.0028538656027519383 86-87 0.0028061021617016967 88-89 0.002089650545948072 90-91 0.002638930118025851 92-93 0.002698634419338653 94-95 0.0022568225896239177 96-97 0.001623956995708216 98-99 0.0020538279651603907 100 0.003009096786165224 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4187303.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.62347512423329 #Duplication Level Percentage of deduplicated Percentage of total 1 77.86941459065253 49.54322762146989 2 14.142660904127341 17.996104684484248 3 4.196149547673786 8.009208490919576 4 1.5394877788283177 3.9179024960137845 5 0.7399280726753549 2.3538397662791155 6 0.4091562938117399 1.5619167168752828 7 0.23883768240201964 1.06369783415241 8 0.1566643861265837 0.797402613886238 9 0.11301998273297634 0.6471651653957516 >10 0.5250999913163505 5.944351767274966 >50 0.0379722389883196 1.678790330686249 >100 0.028938533526740817 3.5114358320769457 >500 0.0020683077429700268 0.904641509234889 >1k 5.26478220594985E-4 0.5612795978548138 >5k 3.7605587185342975E-5 0.1336983841089576 >10k+ 3.7605587185342975E-5 1.3753371892869022 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57483 1.3727929409455202 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5588 0.13345105429437518 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4377 0.10453029073845385 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.1940860262560412E-5 0.0 0.0 0.0 0.0 14-15 2.3881720525120824E-5 0.0 0.0 0.0 0.0 16-17 2.3881720525120824E-5 0.0 0.0 2.3881720525120824E-5 0.0 18-19 2.3881720525120824E-5 0.0 0.0 8.358602183792288E-5 0.0 20-21 2.3881720525120824E-5 0.0 0.0 2.5075806551376863E-4 0.0 22-23 2.3881720525120824E-5 0.0 0.0 8.836236594294705E-4 0.0 24-25 2.3881720525120824E-5 0.0 0.0 0.0024001129127746426 0.0 26-27 4.776344105024165E-5 0.0 0.0 0.004382295716359671 0.0 28-29 4.776344105024165E-5 0.0 0.0 0.009027290358495671 0.0 30-31 4.776344105024165E-5 0.0 0.0 0.020406930188715745 0.0 32-33 4.776344105024165E-5 0.0 0.0 0.04074221521585612 0.0 34-35 4.776344105024165E-5 0.0 0.0 0.07135858092906103 0.0 36-37 4.776344105024165E-5 0.0 0.0 0.117211484337293 0.0 38-39 4.776344105024165E-5 0.0 0.0 0.18153689857170593 0.0 40-41 4.776344105024165E-5 0.0 0.0 0.25522394725196623 0.0 42-43 4.776344105024165E-5 0.0 0.0 0.33250519487125724 0.0 44-45 4.776344105024165E-5 0.0 0.0 0.427232039334149 0.0 46-47 4.776344105024165E-5 0.0 0.0 0.5381029268720223 0.0 48-49 4.776344105024165E-5 0.0 0.0 0.661463954244534 0.0 50-51 4.776344105024165E-5 0.0 0.0 0.791416336481979 0.0 52-53 4.776344105024165E-5 0.0 0.0 0.9203298638765811 0.0 54-55 4.776344105024165E-5 0.0 0.0 1.0647068053111992 0.0 56-57 4.776344105024165E-5 0.0 0.0 1.2402493920310997 0.0 58-59 4.776344105024165E-5 0.0 0.0 1.4363541401231292 0.0 60-61 4.776344105024165E-5 0.0 0.0 1.6395637000713825 0.0 62-63 4.776344105024165E-5 0.0 0.0 1.8495317869282446 0.0 64-65 4.776344105024165E-5 0.0 0.0 2.085519008297226 0.0 66-67 4.776344105024165E-5 0.0 0.0 2.358845299707234 0.0 68-69 4.776344105024165E-5 0.0 0.0 2.658071317026735 0.0 70-71 4.776344105024165E-5 0.0 0.0 2.990433221574842 0.0 72-73 4.776344105024165E-5 0.0 0.0 3.3490291961197935 0.0 74-75 4.776344105024165E-5 0.0 0.0 3.7124015147697698 0.0 76-77 4.776344105024165E-5 0.0 0.0 4.094795623818004 0.0 78-79 4.776344105024165E-5 0.0 0.0 4.523305335200247 0.0 80-81 4.776344105024165E-5 0.0 0.0 4.992748315562547 0.0 82-83 4.776344105024165E-5 0.0 0.0 5.487505919681475 0.0 84-85 4.776344105024165E-5 0.0 0.0 5.983541195848497 0.0 86-87 4.776344105024165E-5 0.0 0.0 6.510730176440539 0.0 88 4.776344105024165E-5 0.0 0.0 6.927227382398646 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7810 0.0 41.23005 1 TACCTGG 4355 0.0 39.07182 2 GTATCAA 12255 0.0 32.64304 1 GTACCTG 5490 0.0 32.109478 1 GTCTCGC 1245 0.0 27.18551 1 ATCAACG 13885 0.0 27.150118 3 TCAACGC 13900 0.0 26.951735 4 ACCTGGG 6100 0.0 26.73886 3 TATCAAC 14820 0.0 26.420439 2 GTACACG 525 0.0 25.966476 1 CCGGTCG 260 1.8189894E-12 25.307919 9 CAACGCA 15130 0.0 24.791752 5 TCTGTCG 1310 0.0 24.755945 8 GTACATG 22605 0.0 24.330761 1 CATGGGT 10550 0.0 23.747503 4 TACATGG 22575 0.0 23.590946 2 AACGCAG 16100 0.0 23.23803 6 ACATGGG 23255 0.0 21.688354 3 CGCAGAG 16965 0.0 21.443161 8 ACGCAGA 17575 0.0 21.073807 7 >>END_MODULE