##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431453_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4187303 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23878520374571 33.0 31.0 34.0 30.0 34.0 2 32.35677284400006 34.0 31.0 34.0 30.0 34.0 3 32.461558191513724 34.0 31.0 34.0 30.0 34.0 4 36.0191326015815 37.0 35.0 37.0 35.0 37.0 5 35.94536339978263 37.0 35.0 37.0 35.0 37.0 6 35.99138657985821 37.0 35.0 37.0 35.0 37.0 7 35.92940276832128 37.0 35.0 37.0 35.0 37.0 8 35.91351617019356 37.0 35.0 37.0 35.0 37.0 9 37.59392000053495 39.0 37.0 39.0 35.0 39.0 10-11 37.53254684459185 39.0 37.0 39.0 34.5 39.0 12-13 37.5130477780089 39.0 37.0 39.0 35.0 39.0 14-15 38.80910158639105 40.0 38.0 41.0 34.0 41.0 16-17 38.86494970629066 40.0 38.0 41.0 35.0 41.0 18-19 38.9735089626903 40.5 38.5 41.0 35.0 41.0 20-21 38.97686016512299 41.0 38.5 41.0 35.0 41.0 22-23 38.868169798077666 40.0 38.0 41.0 35.0 41.0 24-25 38.78771228162853 40.0 38.0 41.0 35.0 41.0 26-27 38.62150422360168 40.0 38.0 41.0 34.0 41.0 28-29 38.499869128171525 40.0 38.0 41.0 34.0 41.0 30-31 38.247242318026665 40.0 37.5 41.0 34.0 41.0 32-33 37.94190043089789 40.0 37.0 41.0 33.5 41.0 34-35 37.72499733121773 40.0 36.5 41.0 33.0 41.0 36-37 37.53337792846612 40.0 36.0 41.0 33.0 41.0 38-39 37.39912480658792 40.0 36.0 41.0 33.0 41.0 40-41 37.239833014233746 40.0 36.0 41.0 33.0 41.0 42-43 37.060418006530696 40.0 36.0 41.0 31.5 41.0 44-45 36.76975095903019 40.0 35.5 41.0 31.0 41.0 46-47 36.328671342866755 40.0 35.0 41.0 30.0 41.0 48-49 35.96175581275107 40.0 35.0 41.0 28.0 41.0 50-51 35.782591563113535 40.0 35.0 41.0 27.0 41.0 52-53 35.63101882046749 40.0 35.0 41.0 27.0 41.0 54-55 35.46796732885105 39.0 35.0 41.0 27.0 41.0 56-57 35.24161769520859 39.0 35.0 41.0 26.0 41.0 58-59 35.07305370545193 39.0 35.0 41.0 26.0 41.0 60-61 34.85265623242454 39.0 35.0 41.0 26.0 41.0 62-63 34.60883891134699 38.0 35.0 41.0 25.5 41.0 64-65 34.357457413518915 37.5 34.5 40.0 25.0 41.0 66-67 34.09325668574736 37.0 34.0 40.0 24.5 41.0 68-69 33.79915055108264 36.5 34.0 40.0 23.0 41.0 70-71 33.46343827996206 36.0 34.0 39.0 22.5 41.0 72-73 33.1404924601826 35.5 34.0 39.0 22.0 41.0 74-75 32.84023236436437 35.0 34.0 39.0 22.0 40.5 76-77 32.49763726197985 35.0 33.0 37.5 20.5 40.0 78-79 32.19116409774979 35.0 33.0 37.0 20.0 39.0 80-81 31.895421945820495 35.0 33.0 36.5 20.0 39.0 82-83 31.650286831404365 35.0 33.0 36.0 19.0 38.5 84-85 31.42272328990761 35.0 33.0 36.0 18.5 37.0 86-87 31.181229063194138 35.0 33.0 35.5 17.0 37.0 88-89 31.031621069695696 35.0 33.0 35.0 14.0 36.5 90-91 30.84369616910933 35.0 33.0 35.0 8.5 36.0 92-93 30.649090954249072 35.0 33.0 35.0 2.0 36.0 94-95 30.48666205908672 35.0 33.0 35.0 2.0 36.0 96-97 30.31911041546313 35.0 33.0 35.0 2.0 36.0 98-99 30.15523094459608 35.0 32.5 35.0 2.0 35.0 100 30.042061441457665 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16135092316627464 1101 2 0.23448294702915007 1101 3 0.21446832589704456 1101 4 0.20106947478257808 1101 5 0.20105525894508958 1101 6 0.17595958374874954 1101 7 0.20354164851574552 1101 8 0.26239811511247524 1101 9 0.34515617655626585 1101 10-11 0.3019341021307014 1101 12-13 0.34847511737496717 1101 14-15 0.46181328330693105 1101 16-17 0.4402918008214911 1101 18-19 0.43906380240691334 1101 20-21 0.37505561564724843 1101 22-23 0.385939345577043 1101 24-25 0.39493389740282225 1101 26-27 0.4224295917989309 1101 28-29 0.407303815020839 1101 30-31 0.42017726603113914 1101 32-33 0.3661824952581796 1101 34-35 0.474808458663297 1101 36-37 0.45771528373808934 1101 38-39 0.44789237039891105 1101 40-41 0.5676130784374962 1101 42-43 0.5320577721977315 1101 44-45 0.5560216004389531 1101 46-47 0.44177699570478524 1101 48-49 0.45708301933173345 1101 50-51 0.366750077036059 1101 52-53 0.38947301099373277 1101 54-55 0.34687499329182003 1101 56-57 0.3961894102735073 1101 58-59 0.37214344420728196 1101 60-61 0.3727021676997637 1101 62-63 0.36942599816008936 1101 64-65 0.3133968559842515 1101 66-67 0.34537295231888265 1101 68-69 0.29557990174428994 1101 70-71 0.31197155593049786 1101 72-73 0.3613403531250512 1101 74-75 0.3373912596020645 1101 76-77 0.2949158239412384 1101 78-79 0.2524126621249678 1101 80-81 0.2359661078159263 1101 82-83 0.2000148947004874 1101 84-85 0.19693048609885722 1101 86-87 0.18417950880338196 1101 88-89 0.22058145861365475 1101 90-91 0.2619740610357404 1101 92-93 0.28757468934443153 1101 94-95 0.31576531278233944 1101 96-97 0.3613367970295549 1101 98-99 0.4393461032247643 1101 100 0.43450890331828873 1102 1 0.09824190242655106 1102 2 0.22053902138115689 1102 3 0.17279713192511537 1102 4 0.15831917487496838 1102 5 0.14919705359304203 1102 6 0.12321959751525213 1102 7 0.15681866551714307 1102 8 0.166359083210196 1102 9 0.2911838032856906 1102 10-11 0.26268801649337803 1102 12-13 0.2939333727404616 1102 14-15 0.454766338125971 1102 16-17 0.41070552277609096 1102 18-19 0.40668900977932054 1102 20-21 0.3178786084408287 1102 22-23 0.30657009163651594 1102 24-25 0.31472377923847716 1102 26-27 0.2241194832142952 1102 28-29 0.25152938661985047 1102 30-31 0.2854432942699461 1102 32-33 0.2616009437921889 1102 34-35 0.28106030285836425 1102 36-37 0.22581550834188846 1102 38-39 0.2917787541061614 1102 40-41 0.3173881386393802 1102 42-43 0.3603497893069232 1102 44-45 0.3677082182307956 1102 46-47 0.3449052596373292 1102 48-49 0.22390009289732404 1102 50-51 0.2712345979866484 1102 52-53 0.24930345108445806 1102 54-55 0.234514229565022 1102 56-57 0.33280697666009473 1102 58-59 0.2592462984445234 1102 60-61 0.26088713880341885 1102 62-63 0.21874110451153683 1102 64-65 0.18537695428488377 1102 66-67 0.2134076259062141 1102 68-69 0.22484711713344296 1102 70-71 0.2104845430638207 1102 72-73 0.1502850475058395 1102 74-75 0.20646332246168697 1102 76-77 0.15335134491792957 1102 78-79 0.1365462295719766 1102 80-81 0.10236760603177686 1102 82-83 0.10848277518242 1102 84-85 0.1464522470261258 1102 86-87 0.09697523129191765 1102 88-89 0.12032758915312414 1102 90-91 0.11529203251324205 1102 92-93 0.11658330796636207 1102 94-95 0.2029077600313407 1102 96-97 0.20356076879162188 1102 98-99 0.21385171203597508 1102 100 0.22417819926929994 1103 1 0.16262180689804495 1103 2 0.2171888102642754 1103 3 0.19652111739545575 1103 4 0.1838154523614719 1103 5 0.13319993017610443 1103 6 0.14032041661450734 1103 7 0.19797086483988124 1103 8 0.19221487687823924 1103 9 0.2888655320388551 1103 10-11 0.26297477041011774 1103 12-13 0.2794787016043543 1103 14-15 0.36520031364972994 1103 16-17 0.3195509476583922 1103 18-19 0.32290518262650636 1103 20-21 0.3204877161160411 1103 22-23 0.3164452053373026 1103 24-25 0.36932484924209774 1103 26-27 0.3335600957679503 1103 28-29 0.3108182147927181 1103 30-31 0.2653393484203832 1103 32-33 0.285370488958435 1103 34-35 0.3056628788551663 1103 36-37 0.3195631445959961 1103 38-39 0.31452278085296115 1103 40-41 0.2741756317742414 1103 42-43 0.33263697733073627 1103 44-45 0.24577343317900358 1103 46-47 0.2641528533085733 1103 48-49 0.16465980125176571 1103 50-51 0.13511823064179396 1103 52-53 0.14119154014127133 1103 54-55 0.1312177261568621 1103 56-57 0.15423987360695435 1103 58-59 0.14776437336790593 1103 60-61 0.20901137212757703 1103 62-63 0.21035984986271927 1103 64-65 0.22948774128993676 1103 66-67 0.18551959196329904 1103 68-69 0.16942897778575627 1103 70-71 0.22990821206011702 1103 72-73 0.1974180696837422 1103 74-75 0.22983358948403065 1103 76-77 0.22930753718653563 1103 78-79 0.28094686356335785 1103 80-81 0.27259433625408036 1103 82-83 0.2514557917083131 1103 84-85 0.26793760141251965 1103 86-87 0.24192845024461462 1103 88-89 0.2799837753319814 1103 90-91 0.27432915116270706 1103 92-93 0.24584389082881586 1103 94-95 0.25082862190398103 1103 96-97 0.2548869982144879 1103 98-99 0.3203078101233032 1103 100 0.27304918067448014 1104 1 0.0806077005541539 1104 2 0.1621900267042662 1104 3 0.15713298671056464 1104 4 0.13085071778518653 1104 5 0.08042202159832357 1104 6 0.06887309718609202 1104 7 0.07257595784303561 1104 8 0.11040874253269095 1104 9 0.16781961600722184 1104 10-11 0.19504902295352622 1104 12-13 0.16330715653372607 1104 14-15 0.28474937629334107 1104 16-17 0.26055802263033456 1104 18-19 0.1948077296512878 1104 20-21 0.2248961338807831 1104 22-23 0.24994130836932982 1104 24-25 0.26980637853824874 1104 26-27 0.29155383960399917 1104 28-29 0.24798205879541513 1104 30-31 0.277737226321193 1104 32-33 0.2932776213951769 1104 34-35 0.34150436756844016 1104 36-37 0.33636625746157023 1104 38-39 0.30751200990905403 1104 40-41 0.34199090773172003 1104 42-43 0.319264312437717 1104 44-45 0.31956844908864923 1104 46-47 0.3593948180466384 1104 48-49 0.3737332445791779 1104 50-51 0.28876059641265783 1104 52-53 0.29961742537386726 1104 54-55 0.3650076277865466 1104 56-57 0.35084289953412906 1104 58-59 0.38902682443781345 1104 60-61 0.3437334635498033 1104 62-63 0.3602368106637073 1104 64-65 0.31810223358102263 1104 66-67 0.29392227109607916 1104 68-69 0.24056750021664186 1104 70-71 0.33609601121062127 1104 72-73 0.2868797099680478 1104 74-75 0.2880531050182711 1104 76-77 0.2368615641261016 1104 78-79 0.3493132980164262 1104 80-81 0.3450750846742352 1104 82-83 0.3291179095870227 1104 84-85 0.2816458773028536 1104 86-87 0.2670301690437995 1104 88-89 0.2390034816598252 1104 90-91 0.2662858232270544 1104 92-93 0.314440751280042 1104 94-95 0.2694506177550089 1104 96-97 0.2816835035001155 1104 98-99 0.3040450647534101 1104 100 0.37670850684520474 1105 1 0.03813919574163549 1105 2 0.10871030607207643 1105 3 0.10246959136016898 1105 4 0.0789975138120198 1105 5 0.03917543645977872 1105 6 0.08431846249444419 1105 7 0.10325619461956137 1105 8 0.14728570155392617 1105 9 0.17472948008224876 1105 10-11 0.15686427300570926 1105 12-13 0.06445511286187866 1105 14-15 0.19511418040134032 1105 16-17 0.14608699221155064 1105 18-19 0.15486912200008618 1105 20-21 0.15178083997083291 1105 22-23 0.11869697315354699 1105 24-25 0.15215864480373398 1105 26-27 0.18760371667645614 1105 28-29 0.17744720668692082 1105 30-31 0.21325817645488598 1105 32-33 0.1982341223553803 1105 34-35 0.12806443981329352 1105 36-37 0.14983902524075887 1105 38-39 0.19084517267254597 1105 40-41 0.1871819763325604 1105 42-43 0.28774567160641595 1105 44-45 0.27351857734650054 1105 46-47 0.28308607032987254 1105 48-49 0.27430568761471363 1105 50-51 0.20821496055921784 1105 52-53 0.2191471467416406 1105 54-55 0.2526108598504351 1105 56-57 0.32401915350494903 1105 58-59 0.2691286482344779 1105 60-61 0.24586372890743036 1105 62-63 0.2622261027157222 1105 64-65 0.2935758900290537 1105 66-67 0.2710829698439454 1105 68-69 0.28767150688081244 1105 70-71 0.2653187233947065 1105 72-73 0.2959567171326256 1105 74-75 0.32436840954620294 1105 76-77 0.30758594864083477 1105 78-79 0.28423616100556615 1105 80-81 0.3150116977244082 1105 82-83 0.294990976097953 1105 84-85 0.26759750561582507 1105 86-87 0.2825055439829569 1105 88-89 0.2844629818774109 1105 90-91 0.26124124693212636 1105 92-93 0.293035622543524 1105 94-95 0.30090124386357786 1105 96-97 0.2500320180820239 1105 98-99 0.28103776553702886 1105 100 0.3321225907114105 1106 1 0.07358909034687855 1106 2 0.11933778334614686 1106 3 0.10528603647224344 1106 4 0.14720308124331183 1106 5 0.09523296321768271 1106 6 0.06937046899930976 1106 7 0.11265422318786733 1106 8 0.12555164597166169 1106 9 0.1551556826105127 1106 10-11 0.15423134327737387 1106 12-13 0.1463451324261129 1106 14-15 0.22871550829871268 1106 16-17 0.17717318141043137 1106 18-19 0.17272687089367622 1106 20-21 0.17430506952897673 1106 22-23 0.11292387269238446 1106 24-25 0.14388220793935602 1106 26-27 0.19543024901444284 1106 28-29 0.1278690884928153 1106 30-31 0.14895454318529033 1106 32-33 0.1498173964002163 1106 34-35 0.18934443068892648 1106 36-37 0.16171069120508008 1106 38-39 0.11530409219405868 1106 40-41 0.1380500003611118 1106 42-43 0.05531387128558407 1106 44-45 -0.03527640547201116 1106 46-47 -0.055555886453575454 1106 48-49 -0.015498165195616309 1106 50-51 -0.01671953476935073 1106 52-53 -0.04475324487552257 1106 54-55 -0.05751184253193742 1106 56-57 -0.0766363274204167 1106 58-59 -0.05134518248210895 1106 60-61 -0.04264163950057309 1106 62-63 -0.04755077695376286 1106 64-65 -0.06817914838496364 1106 66-67 -0.0762613500862841 1106 68-69 -0.08556056791296385 1106 70-71 -0.055061436266647945 1106 72-73 -0.01713879788952255 1106 74-75 -0.08243707221279806 1106 76-77 -0.029369272033612503 1106 78-79 -0.003248579438377419 1106 80-81 -0.06033466292059941 1106 82-83 -0.10282450666320742 1106 84-85 -0.08630938009834921 1106 86-87 -0.053898331694963275 1106 88-89 -0.05991139867925099 1106 90-91 -0.028773037195144013 1106 92-93 -0.03578031785292879 1106 94-95 -0.0226714237831942 1106 96-97 -0.003209947447491146 1106 98-99 0.06474370995888279 1106 100 0.1380442815546168 1107 1 0.06704856479884569 1107 2 0.15621586220908767 1107 3 0.1340410500360676 1107 4 0.11389545992947347 1107 5 0.10785891281307869 1107 6 0.12440218761035737 1107 7 0.06370242868260334 1107 8 0.06579037578877234 1107 9 0.13239316647931787 1107 10-11 0.09213573363684446 1107 12-13 0.06897420653583453 1107 14-15 0.20780149515683632 1107 16-17 0.2006444558501741 1107 18-19 0.15198384122970054 1107 20-21 0.19044258250267632 1107 22-23 0.19244635830760615 1107 24-25 0.1871974831311647 1107 26-27 0.1950608570105885 1107 28-29 0.2057551672820921 1107 30-31 0.18319746565017425 1107 32-33 0.18492835149991294 1107 34-35 0.21835642342353623 1107 36-37 0.2672139675899601 1107 38-39 0.23947122123326636 1107 40-41 0.22707342351727533 1107 42-43 0.2609495195675535 1107 44-45 0.3152848685434151 1107 46-47 0.34634658315707156 1107 48-49 0.3270433478532482 1107 50-51 0.3092678758394882 1107 52-53 0.3262041020820732 1107 54-55 0.4009083004939171 1107 56-57 0.4066655015341354 1107 58-59 0.3766017627420837 1107 60-61 0.33724458885724573 1107 62-63 0.3631702019263301 1107 64-65 0.3699181007856609 1107 66-67 0.3807867690519089 1107 68-69 0.3205492823455245 1107 70-71 0.3786114209866014 1107 72-73 0.373274604845939 1107 74-75 0.3627441888143039 1107 76-77 0.350635541037434 1107 78-79 0.33472000594055373 1107 80-81 0.35958866391193567 1107 82-83 0.3850976465166376 1107 84-85 0.42566478909005667 1107 86-87 0.4155519939401522 1107 88-89 0.3956037921718334 1107 90-91 0.4241062008862535 1107 92-93 0.45938427536479765 1107 94-95 0.4720606276489754 1107 96-97 0.45524860806626677 1107 98-99 0.4773341214702356 1107 100 0.5055776037968158 1108 1 0.07333973685406647 1108 2 0.1246171091190007 1108 3 0.08539163621860268 1108 4 0.1111139103565364 1108 5 0.07114870393562 1108 6 0.032884322059615556 1108 7 0.03507708549987143 1108 8 0.08271199555658626 1108 9 0.07556058740376415 1108 10-11 0.05517054128137033 1108 12-13 0.060620748523668055 1108 14-15 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92-93 -0.1089979486648538 2214 94-95 -0.011761689254139895 2214 96-97 -0.07912907384868717 2214 98-99 -0.09065541713259506 2214 100 -0.05409355252726655 2215 1 -0.14999285220773118 2215 2 -0.18658773705948306 2215 3 -0.23836809998567787 2215 4 -0.16930689429806733 2215 5 -0.17470295303933625 2215 6 -0.25048444507076795 2215 7 -0.24864148925249197 2215 8 -0.21646121584565492 2215 9 -0.2649711460693638 2215 10-11 -0.23729435660781206 2215 12-13 -0.27021911336543525 2215 14-15 -0.3363307942878109 2215 16-17 -0.3950971619800683 2215 18-19 -0.351062641836144 2215 20-21 -0.4205037319501628 2215 22-23 -0.3945869841192149 2215 24-25 -0.4533944262933005 2215 26-27 -0.38099385073235226 2215 28-29 -0.39874320629814974 2215 30-31 -0.3960326774024594 2215 32-33 -0.3804411235209315 2215 34-35 -0.3851546492685074 2215 36-37 -0.32417789494278537 2215 38-39 -0.32983195458550085 2215 40-41 -0.31838596748191605 2215 42-43 -0.2912681413022895 2215 44-45 -0.20332599697046305 2215 46-47 -0.1269159853042936 2215 48-49 -0.15022041043086887 2215 50-51 -0.08132162326266723 2215 52-53 -0.09287310601968102 2215 54-55 -0.008751011402885922 2215 56-57 -0.04671804319542616 2215 58-59 -0.11478757990823851 2215 60-61 -0.20412163661087135 2215 62-63 -0.08394749082550135 2215 64-65 -0.008990045117727163 2215 66-67 -0.04053861240898726 2215 68-69 -0.1575739201839923 2215 70-71 -0.08179174924460142 2215 72-73 -0.04679768291148889 2215 74-75 -0.09204851798860858 2215 76-77 -0.06280008856614216 2215 78-79 -0.005720874878861082 2215 80-81 -0.027591762956145516 2215 82-83 -0.04088184821407381 2215 84-85 -0.014423965551181084 2215 86-87 -0.014149421905088388 2215 88-89 0.0025484785615113026 2215 90-91 -0.012301005539228527 2215 92-93 -0.026426978868332895 2215 94-95 -0.09641788925779338 2215 96-97 -0.10121439732080262 2215 98-99 -0.15212883680598566 2215 100 -0.1524057671704142 2216 1 -0.18363945484266964 2216 2 -0.29816190844985613 2216 3 -0.28001248393962186 2216 4 -0.09876360320500766 2216 5 -0.2879006602979217 2216 6 -0.34239060441574765 2216 7 -0.3420621293270898 2216 8 -0.38626676027779894 2216 9 -0.7396747771827279 2216 10-11 -0.540629150011732 2216 12-13 -0.4352825794634114 2216 14-15 -0.6859973658346021 2216 16-17 -0.4679363847214759 2216 18-19 -0.5088411385584166 2216 20-21 -0.7374049885111802 2216 22-23 -0.6588258822004178 2216 24-25 -0.5462327996102587 2216 26-27 -0.7032196285330983 2216 28-29 -0.6618092919115242 2216 30-31 -0.6716782106237531 2216 32-33 -0.6651957749026494 2216 34-35 -0.7213005952631093 2216 36-37 -0.6640032384018824 2216 38-39 -0.705451887641459 2216 40-41 -0.944522718554758 2216 42-43 -0.9922528865003954 2216 44-45 -0.9533008665329206 2216 46-47 -0.9541847020979688 2216 48-49 -0.6660789691259055 2216 50-51 -0.751553719685262 2216 52-53 -0.8393875299905176 2216 54-55 -0.6946455160181557 2216 56-57 -0.9798476908095353 2216 58-59 -1.016436950805712 2216 60-61 -0.8160044583127686 2216 62-63 -0.9714121193432206 2216 64-65 -0.9943148873381134 2216 66-67 -0.902159012844109 2216 68-69 -0.8077279909038708 2216 70-71 -0.8665225815122355 2216 72-73 -0.7176536952903803 2216 74-75 -0.7550848933183474 2216 76-77 -0.668071784999853 2216 78-79 -0.6198833639038668 2216 80-81 -0.6812296090819281 2216 82-83 -0.7790231979221183 2216 84-85 -0.7711517986067875 2216 86-87 -0.71187959929388 2216 88-89 -0.7592465627569247 2216 90-91 -0.8117128867560268 2216 92-93 -0.7169168305948368 2216 94-95 -0.7884810551214194 2216 96-97 -0.9381715240897712 2216 98-99 -0.9863498774018211 2216 100 -1.0952584084694301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 361.0 3 961.0 4 661.0 5 586.0 6 1039.0 7 2338.0 8 3857.0 9 5778.0 10 7487.0 11 10065.0 12 14205.0 13 20364.0 14 20537.0 15 24761.0 16 32557.0 17 47124.0 18 53422.0 19 29934.0 20 18928.0 21 16501.0 22 16582.0 23 16979.0 24 18734.0 25 21856.0 26 26563.0 27 33125.0 28 41580.0 29 52311.0 30 65271.0 31 81030.0 32 102426.0 33 130058.0 34 176091.0 35 276778.0 36 404277.0 37 756765.0 38 1116609.0 39 535683.0 40 3119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.04033027078391 20.516460854711966 14.359424776234302 23.08378409826982 2 14.750974553310328 21.930583958218453 39.54927551218529 23.76916597628593 3 16.268048052035518 28.100319681398055 31.73132815608362 23.90030411048281 4 11.046414647587358 18.737707605547907 38.10567002302307 32.11020772384167 5 10.934645619456926 37.70384956634612 34.87255483813957 16.488949976057384 6 26.962534118022987 39.02141784341854 18.800574021034542 15.215474017523928 7 23.915489277943344 34.48701944903438 23.39618126512459 18.201310007897682 8 25.153446024804033 33.078642744506425 21.93299601199149 19.83491521869805 9 25.273212853237514 15.435997824852896 23.422976555553777 35.86781276635582 10-11 22.641313174739658 26.812482086557754 29.372336868252603 21.173867870449985 12-13 24.720613722006743 25.28564328877084 29.75809250011284 20.235650489109577 14-15 22.07658485664878 26.330420798303827 29.504659681900257 22.088334663147137 16-17 18.90148623111344 29.384164460990764 29.928619925522465 21.785729382373333 18-19 18.635981203175408 26.16268753419564 35.094725650376866 20.10660561225209 20-21 20.613566775559352 25.431560601179328 34.88044691296522 19.074425710296104 22-23 19.965190004162583 25.790670032715568 35.01945046728168 19.224689495840163 24-25 19.50162856375567 25.618895423363885 35.27815262347608 19.60132338940436 26-27 19.343763754378415 25.442235730254055 35.34713155460687 19.866868960760662 28-29 19.346366440306788 25.558171941962392 35.384861551100535 19.71060006663029 30-31 19.947185995619453 26.226270536955116 34.70835869865567 19.118184768769762 32-33 19.125954046249884 25.728411431049675 35.50078965022065 19.644844872479787 34-35 19.37356617483747 25.259097550502407 35.147672946241734 20.219663328418385 36-37 20.186349644350578 25.91509600014712 34.22649505186012 19.672059303642186 38-39 19.96421515138706 25.830277029274846 34.505666849841916 19.699840969496186 40-41 19.55334880331349 25.740895067275073 34.57046206585015 20.135294063561297 42-43 19.512784312938784 25.887727697233114 34.3462006965717 20.2532872932564 44-45 20.17284397293962 26.090412622446074 33.66432207847415 20.07242132614016 46-47 20.019387271007563 26.7965018370502 32.86698882461624 20.31712206732599 48-49 20.115117593542447 26.37910712279214 32.67961197277424 20.826163310891175 50-51 20.194849182820608 26.325751446926176 32.29226135554495 21.18713801470826 52-53 20.25863911581384 26.67342605420295 31.953521906792098 21.114412923191114 54-55 20.522406060287636 27.287032570224923 31.568113754579368 20.62244761490808 56-57 20.04699972808989 26.795827750140283 31.70685630237359 21.450316219396232 58-59 19.95387535837834 26.14216525958486 32.30917458100425 21.59478480103255 60-61 20.473479529314666 26.769201596463173 31.58582852350083 21.17149035072133 62-63 20.215513947267862 26.57003486816966 31.928293370271305 21.286157814291172 64-65 20.057627217815003 26.30653834878796 32.31660579747006 21.319228635926976 66-67 20.028307016858832 26.41552398549806 31.804615908911256 21.75155308873186 68-69 20.689833463151363 26.597188646592418 31.524275414192747 21.18870247606347 70-71 20.771984895220864 26.29028745124525 31.510401491754557 21.42732616177933 72-73 20.410846790881866 26.428861250308373 31.85508667512239 21.305205283687375 74-75 20.392377652146347 26.45562735347483 31.529514596842713 21.62248039753612 76-77 20.49618811917838 26.592140573538625 31.12824651094034 21.783424796342658 78-79 20.72130109267817 26.722105194529 31.238365808070416 21.31822790472241 80-81 20.299784849579787 26.548389261536602 31.385428759275364 21.766397129608247 82-83 20.123269316445896 26.61814561307415 31.487159426301886 21.771425644178063 84-85 20.30826730988531 26.498307371739376 31.16098178409586 22.03244353427945 86-87 20.487841457854852 26.64398778879866 30.917096278917477 21.951074474429007 88-89 20.07397124115451 26.556174702427793 31.067634704247578 22.30221935217012 90-91 19.877615734996965 26.420777287910617 30.94807087043856 22.753536106653854 92-93 20.031655337733557 26.705152201753524 30.741595842177066 22.521596618335856 94-95 19.905163299622693 26.42359533093258 31.05999255367954 22.611248815765183 96-97 19.796629364019278 26.57306734370108 30.908601548355602 22.72170174392404 98-99 19.70108244622099 26.499883575833927 30.87481715435456 22.92421682359052 100 19.886145850523263 26.680282483622452 30.608100185155685 22.8254714806986 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4587.0 1 2987.0 2 10877.5 3 18421.0 4 10442.5 5 4567.0 6 7873.5 7 17681.5 8 27362.5 9 28578.5 10 18976.5 11 9126.5 12 6845.0 13 6782.5 14 7346.5 15 7996.0 16 8512.5 17 9115.0 18 9857.5 19 10613.0 20 11529.5 21 13028.0 22 15389.0 23 19199.0 24 23597.0 25 28777.5 26 36683.0 27 46624.0 28 59206.5 29 71359.5 30 79809.5 31 88357.5 32 97014.0 33 105398.0 34 113780.0 35 119525.5 36 123910.0 37 127937.0 38 130072.0 39 130618.0 40 131279.0 41 130671.5 42 127151.5 43 125057.5 44 123609.0 45 121640.5 46 121053.5 47 120827.0 48 120768.0 49 121911.0 50 125021.0 51 121550.5 52 112533.5 53 107032.0 54 104130.5 55 101252.0 56 93511.5 57 84078.0 58 78206.5 59 72808.5 60 62897.5 61 51159.0 62 41791.5 63 32233.5 64 23717.5 65 17555.0 66 12774.5 67 9219.5 68 6653.5 69 4761.0 70 3393.5 71 2484.0 72 1885.5 73 1379.5 74 1035.5 75 781.0 76 473.0 77 341.0 78 275.5 79 170.5 80 83.0 81 26.0 82 15.5 83 12.5 84 6.0 85 3.0 86 2.0 87 1.5 88 2.0 89 0.5 90 0.0 91 0.0 92 0.5 93 1.0 94 1.5 95 1.5 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.048002258255492855 2 0.0 3 2.1493548472608742E-4 4 0.06698822607296391 5 0.004967397869225131 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.012012505424135775 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.851021528654602E-4 26-27 0.0 28-29 4.2987096945217484E-4 30-31 0.003725548401918849 32-33 1.4329032315072492E-4 34-35 0.0018388924804343034 36-37 0.004155419371371024 38-39 0.0013373763494067663 40-41 0.0012776720480939642 42-43 0.001946360222797347 44-45 1.1940860262560413E-5 46-47 4.656935502398561E-4 48-49 4.656935502398561E-4 50-51 0.001540370973870293 52-53 9.552688210048329E-4 54-55 0.0010269139825801953 56-57 0.006149543035218611 58-59 0.00924222584322176 60-61 0.0011224408646806788 62-63 0.002459817214087445 64-65 0.0010866182838929974 66-67 4.7763441050241654E-5 68-69 7.52274196541306E-4 70-71 4.537526899772956E-4 72-73 0.0 74-75 2.507580655137687E-4 76-77 0.0 78-79 5.373387118152186E-4 80-81 0.0 82-83 0.00115826344546836 84-85 6.686881747033831E-4 86-87 0.0 88-89 0.0 90-91 0.0 92-93 3.701666681393728E-4 94-95 0.0 96-97 5.253978515526581E-4 98-99 6.686881747033831E-4 100 3.8210752840193323E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4187303.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.10518516640185 #Duplication Level Percentage of deduplicated Percentage of total 1 74.3942353713854 42.48296586195846 2 16.10006126534013 18.38793959495324 3 4.8745522252938045 8.350866222860965 4 1.8862661326936951 4.3086230712234475 5 0.8698168043928206 2.4835524837849974 6 0.48728762652112295 1.6695990085071115 7 0.2957048358600699 1.182039558447283 8 0.19410829141484703 0.8867671938862983 9 0.14399008416656156 0.7400322376611557 >10 0.660538310482304 6.737434482658655 >50 0.05125663319516415 2.0470661432026285 >100 0.03803864349221005 4.283832943773573 >500 0.002678804617153696 1.040746535755252 >1k 0.0011301205973763447 0.9790789728030097 >5k 1.2556895526403023E-4 0.6041262886967184 >10k+ 2.0928159210671708E-4 3.815329399827351 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46498 1.110452240977068 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39940 0.9538359177733257 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29169 0.6966059059972494 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29067 0.694169970503687 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14949 0.3570078401300312 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9848 0.23518718373138986 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8899 0.2125234309530502 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6528 0.15589987158798874 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1940860262560412E-5 0.0 12-13 0.0 0.0 0.0 2.3881720525120824E-5 0.0 14-15 0.0 0.0 0.0 2.3881720525120824E-5 0.0 16-17 0.0 0.0 0.0 4.776344105024165E-5 0.0 18-19 0.0 0.0 0.0 1.0746774236304371E-4 0.0 20-21 0.0 0.0 0.0 2.746397860388895E-4 0.0 22-23 0.0 0.0 0.0 9.075053799545913E-4 0.0 24-25 4.776344105024165E-5 0.0 0.0 0.0024836989346125656 0.0 26-27 5.970430131280206E-5 0.0 0.0 0.004465881738197594 0.0 28-29 7.164516157536247E-5 0.0 0.0 0.009182521541908956 0.0 30-31 7.164516157536247E-5 0.0 0.0 0.02063380653370439 0.0 32-33 7.164516157536247E-5 0.0 0.0 0.04116014532504574 0.0 34-35 7.164516157536247E-5 0.0 0.0 0.07215861856665257 0.0 36-37 7.164516157536247E-5 0.0 0.0 0.11875185531116329 0.0 38-39 7.164516157536247E-5 0.0 0.0 0.184116124388419 0.0 40-41 7.164516157536247E-5 0.0 0.0 0.25904502253598555 0.0 42-43 8.358602183792288E-5 0.0 0.0 0.3379382862907222 0.0 44-45 1.0746774236304371E-4 0.0 0.0 0.43486224904192505 0.0 46-47 1.1940860262560411E-4 0.0 0.0 0.5481571312130983 0.0 48-49 1.1940860262560411E-4 0.0 0.0 0.6737869220354964 0.0 50-51 1.1940860262560411E-4 0.0 0.0 0.8066170515962183 0.0 52-53 1.1940860262560411E-4 0.0 0.0 0.9384799714756731 0.0 54-55 1.1940860262560411E-4 0.0 0.0 1.0857227193733054 0.0 56-57 1.1940860262560411E-4 0.0 0.0 1.264990854495125 0.0 58-59 1.1940860262560411E-4 0.0 0.0 1.4648211509890734 0.0 60-61 1.1940860262560411E-4 0.0 0.0 1.6721622485881724 0.0 62-63 1.1940860262560411E-4 0.0 0.0 1.8863454591177184 0.0 64-65 1.1940860262560411E-4 0.0 0.0 2.126273164373345 0.0 66-67 1.1940860262560411E-4 0.0 0.0 2.404698203115466 0.0 68-69 1.1940860262560411E-4 0.0 0.0 2.7081274032473885 0.0 70-71 1.1940860262560411E-4 0.0 0.0 3.0449910121144805 0.0 72-73 1.1940860262560411E-4 0.0 0.0 3.408829024314696 0.0 74-75 1.1940860262560411E-4 0.0 0.0 3.7780881870741143 0.0 76-77 1.1940860262560411E-4 0.0 0.0 4.166297495070216 0.0 78-79 1.1940860262560411E-4 0.0 0.0 4.601219448413454 0.0 80-81 1.1940860262560411E-4 0.0 0.0 5.079462842789261 0.0 82-83 1.1940860262560411E-4 0.0 0.0 5.583844780279812 0.0 84-85 1.1940860262560411E-4 0.0 0.0 6.089337217774783 0.0 86-87 1.1940860262560411E-4 0.0 0.0 6.628478999489648 0.0 88 1.1940860262560411E-4 0.0 0.0 7.054254253871764 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15510 0.0 60.245987 1 GTATCAA 28910 0.0 42.883797 1 TACCTGG 4295 0.0 40.952187 2 ATAACGC 530 0.0 32.831783 3 TATAACG 510 0.0 32.275017 2 TATCAAC 38375 0.0 32.169888 2 GTGGTAT 5465 0.0 32.11289 1 ATCAACG 38565 0.0 32.09676 3 TGGTATC 5510 0.0 32.092598 2 TCAACGC 39140 0.0 31.649261 4 GTACCTG 5780 0.0 30.606995 1 CAACGCA 40640 0.0 30.46727 5 ACCTGGG 5640 0.0 29.851938 3 AACGCAG 42325 0.0 29.319576 6 TATCACG 390 0.0 26.529358 2 CGCAGAG 46525 0.0 26.501022 8 ACGCAGA 46800 0.0 26.425653 7 TCTGTCG 1170 0.0 26.11429 8 CATGGGT 9810 0.0 25.024773 4 GCAGAGT 48325 0.0 24.998386 9 >>END_MODULE