FastQCFastQC Report
Wed 25 May 2016
SRR2431452_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431452_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6070143
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA346490.5708102758040462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121800.044.694071
GTATCAA205600.035.5088771
TACCTGG47950.029.9987752
GTACCTG52950.028.2325271
GTACATG465750.027.4034731
ATCAACG256500.027.3617043
TCAACGC256950.027.1855014
TACATGG457600.026.3186632
ACCTGGG53550.025.8082523
CATGGGT166950.025.6506924
TATCAAC281250.025.5890032
CAACGCA284900.024.5017765
ACATGGG479400.024.327613
AACGCAG293150.023.7785956
ATGGGTG96500.022.1152635
ACGCAGA307550.022.1146767
CGCAGAG305500.022.015458
CATGGGG175500.020.8654374
ATGGGTA68400.020.6171535
GCAGAGT318950.020.2471229
Produced by FastQC (version 0.11.5)