FastQCFastQC Report
Wed 25 May 2016
SRR2431452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6070143
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT664611.0948835966467347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT512960.8450542268938309No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488860.8053517025875667No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148310.24432702821004382No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109220.17992986326681265No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95910.15800286747775133No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90850.1496669847810834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA218750.059.8571851
GTATCAA437150.045.3647231
TAACGCA5900.031.8824354
ATCAACG631100.031.259163
TATCAAC634800.031.0695532
TCAACGC639850.030.8537394
CAACGCA665000.029.705115
AACGCAG680650.029.0899756
TATAACG6950.027.0656642
ACGCAGA745700.026.3569347
CGCAGAG747350.026.216978
TACCTGG44100.025.5927032
CATGGGT168100.025.4296074
GCAGAGT776800.025.0051789
GTACATG449900.024.6696571
GGGTACG2900.024.314887
TACATGG446700.024.287072
AGTACTT530900.024.13957412-13
GTACCTG47200.023.9232621
AGAGTAC765750.023.7698910-11
Produced by FastQC (version 0.11.5)