FastQCFastQC Report
Wed 25 May 2016
SRR2431451_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431451_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924803
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA274810.46382976784207003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91350.046.881951
GTATCAA154250.034.9571
ATCAACG176550.029.260263
TCAACGC177100.029.169394
TATCAAC196200.027.0724562
CAACGCA207300.025.0330815
GTACATG312550.024.4115241
TACATGG307850.023.8958992
AACGCAG218300.023.7485436
GTGGTAT46750.022.524871
ACGCAGA226450.022.3954947
CGCAGAG223950.022.3301228
ACATGGG323550.021.9082723
CATGGGT78950.020.6597734
TGGTATC49400.020.5529882
GCAGAGT241650.019.8387349
CATGGGA144250.018.4111464
CAGAGTA235250.017.85089310-11
CATGGGG133850.017.5238844
TACCTGG40100.017.3486442
Produced by FastQC (version 0.11.5)