FastQCFastQC Report
Wed 25 May 2016
SRR2431451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431451_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924803
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436560.7368346255563265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378680.6391436137201524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267610.451677465056644No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94950.16025849298280465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84020.14181062222659555No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73440.12395348841134465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170000.062.548241
GTATCAA324150.042.9055981
GTGGTAT57050.036.864421
TGGTATC60900.033.6678732
ATCAACG429600.032.1503833
TCAACGC435150.031.772754
TATCAAC437400.031.6953242
CAACGCA462450.029.8443245
AACGCAG478350.028.8810716
ACGCAGA528450.026.0451377
CGCAGAG529200.025.8927528
GCAGAGT561350.024.2004599
GTACATG296150.023.7193971
TACATGG293600.023.2251452
GAGTACT388200.022.34146712-13
CATGGGT74250.022.294244
ACATGGG301750.022.2082373
CAGAGTA553600.021.4569810-11
AGTACTT389550.021.4376612-13
AGAGTAC539800.021.20873310-11
Produced by FastQC (version 0.11.5)