##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431451_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5924803 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25228872588675 33.0 31.0 34.0 30.0 34.0 2 32.39833864518364 34.0 31.0 34.0 31.0 34.0 3 32.502104458156666 34.0 31.0 34.0 31.0 34.0 4 36.048377979824814 37.0 35.0 37.0 35.0 37.0 5 35.95658336656932 37.0 35.0 37.0 35.0 37.0 6 36.000138232444186 37.0 35.0 37.0 35.0 37.0 7 35.939716476649096 37.0 35.0 37.0 35.0 37.0 8 35.92581137296886 37.0 35.0 37.0 35.0 37.0 9 37.61283911043118 39.0 37.0 39.0 35.0 39.0 10-11 37.55213329455849 39.0 37.0 39.0 35.0 39.0 12-13 37.529231858004394 39.0 37.0 39.0 35.0 39.0 14-15 38.85849926149443 40.0 38.0 41.0 35.0 41.0 16-17 38.91369679295666 40.0 38.0 41.0 35.0 41.0 18-19 39.00139413918066 40.5 38.5 41.0 35.0 41.0 20-21 39.029876183225 41.0 39.0 41.0 35.0 41.0 22-23 38.9816470691093 41.0 39.0 41.0 35.0 41.0 24-25 38.92054073359064 41.0 38.0 41.0 35.0 41.0 26-27 38.77511589836827 40.0 38.0 41.0 34.5 41.0 28-29 38.68197086046574 40.0 38.0 41.0 34.0 41.0 30-31 38.49342172220747 40.0 38.0 41.0 34.0 41.0 32-33 38.28833051495552 40.0 38.0 41.0 34.0 41.0 34-35 38.14280027538469 40.0 37.5 41.0 34.0 41.0 36-37 37.997268769948974 40.0 37.0 41.0 33.0 41.0 38-39 37.88931640427538 40.0 37.0 41.0 33.0 41.0 40-41 37.750771375858406 40.0 37.0 41.0 33.0 41.0 42-43 37.60034105775331 40.0 37.0 41.0 33.0 41.0 44-45 37.35089563315439 40.0 37.0 41.0 32.5 41.0 46-47 36.98578720676451 40.0 36.5 41.0 31.0 41.0 48-49 36.695577726381785 40.0 36.0 41.0 31.0 41.0 50-51 36.5359223758157 40.0 36.0 41.0 30.0 41.0 52-53 36.384723340168435 40.0 36.0 41.0 30.0 41.0 54-55 36.220091199656764 40.0 35.0 41.0 30.0 41.0 56-57 35.98308154718393 39.0 35.0 41.0 29.0 41.0 58-59 35.801551629649126 39.0 35.0 41.0 29.0 41.0 60-61 35.590281060821766 39.0 35.0 41.0 28.5 41.0 62-63 35.36447895398379 38.5 35.0 41.0 28.0 41.0 64-65 35.12130555226899 38.0 35.0 40.5 28.0 41.0 66-67 34.854316421997495 37.0 35.0 40.0 28.0 41.0 68-69 34.5662007665065 37.0 34.5 40.0 28.0 41.0 70-71 34.246280846806215 36.0 34.0 39.0 27.5 41.0 72-73 33.92493058418989 36.0 34.0 39.0 26.0 41.0 74-75 33.619227255319714 35.0 34.0 39.0 26.0 40.5 76-77 33.278848343143224 35.0 34.0 37.5 26.0 40.0 78-79 32.97309800849075 35.0 34.0 37.0 26.0 39.0 80-81 32.68295545016433 35.0 34.0 37.0 26.0 39.0 82-83 32.44363913871904 35.0 33.5 36.0 26.0 38.5 84-85 32.228333077066026 35.0 33.0 36.0 25.5 37.0 86-87 32.00177643037245 35.0 33.0 35.5 25.0 37.0 88-89 31.864410597280617 35.0 33.0 35.0 25.0 36.5 90-91 31.685481694496847 35.0 33.0 35.0 25.0 36.0 92-93 31.502501686553966 35.0 33.0 35.0 24.5 36.0 94-95 31.349839479894946 35.0 33.0 35.0 24.0 36.0 96-97 31.195641019625462 35.0 33.0 35.0 23.5 36.0 98-99 31.040841357932067 35.0 33.0 35.0 21.0 35.0 100 30.936527678641806 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13879399647705526 1101 2 0.2284289178449157 1101 3 0.18226004874198765 1101 4 0.14845796439688286 1101 5 0.16841453837735543 1101 6 0.1725300122650566 1101 7 0.21094552906125585 1101 8 0.24975379688683574 1101 9 0.3661993577940166 1101 10-11 0.3072933716909816 1101 12-13 0.3206075479257464 1101 14-15 0.40553056093789763 1101 16-17 0.3792559461876692 1101 18-19 0.3592822623752525 1101 20-21 0.31082970487427275 1101 22-23 0.3260518964020278 1101 24-25 0.3556421366722944 1101 26-27 0.34815219021673016 1101 28-29 0.3599081741822161 1101 30-31 0.3994743899879367 1101 32-33 0.4415190615516664 1101 34-35 0.4517046856862237 1101 36-37 0.457613182620797 1101 38-39 0.37670382820825665 1101 40-41 0.44535545009224364 1101 42-43 0.4044274686908551 1101 44-45 0.505257654774816 1101 46-47 0.46716477211536045 1101 48-49 0.4119477768767794 1101 50-51 0.3198874796613467 1101 52-53 0.3646523612328352 1101 54-55 0.3439880490378897 1101 56-57 0.37527253797772886 1101 58-59 0.43935881211316996 1101 60-61 0.44764057949670644 1101 62-63 0.45910524590707524 1101 64-65 0.43407387888112225 1101 66-67 0.37367271713368666 1101 68-69 0.3636141884871904 1101 70-71 0.3818813626900166 1101 72-73 0.4009695191818281 1101 74-75 0.36510687535204767 1101 76-77 0.4320270918975524 1101 78-79 0.39151171369657334 1101 80-81 0.39989955125222565 1101 82-83 0.3926355930276699 1101 84-85 0.38265061837046943 1101 86-87 0.38819165772362396 1101 88-89 0.3696389741975743 1101 90-91 0.43779204696856766 1101 92-93 0.42273796042583456 1101 94-95 0.4427909337113256 1101 96-97 0.45531196487664616 1101 98-99 0.4608158966706526 1101 100 0.4845689347508504 1102 1 0.18116003271437364 1102 2 0.27304061591385675 1102 3 0.22909044785792076 1102 4 0.18463098085611307 1102 5 0.17069987170611256 1102 6 0.13708915923462683 1102 7 0.14914907964265467 1102 8 0.1545802919228194 1102 9 0.3023471595422649 1102 10-11 0.2976753426756318 1102 12-13 0.3223971414763014 1102 14-15 0.4507549461363496 1102 16-17 0.4335542407487125 1102 18-19 0.40292055736819776 1102 20-21 0.33952631542863543 1102 22-23 0.30522873658663485 1102 24-25 0.34063465560432604 1102 26-27 0.34759936218655696 1102 28-29 0.3411866581082492 1102 30-31 0.3431708815718011 1102 32-33 0.36663326114601347 1102 34-35 0.407475804370101 1102 36-37 0.39246370002376807 1102 38-39 0.38064936091399915 1102 40-41 0.41015130659348387 1102 42-43 0.4537116070806988 1102 44-45 0.38591332258487654 1102 46-47 0.48713035467260113 1102 48-49 0.4540946083006645 1102 50-51 0.4297625949319013 1102 52-53 0.41744456830820553 1102 54-55 0.42043119760540293 1102 56-57 0.5011987981646087 1102 58-59 0.510359950491619 1102 60-61 0.5494953451364566 1102 62-63 0.5392207788067793 1102 64-65 0.5456509981384414 1102 66-67 0.5359616868697401 1102 68-69 0.5368501604017126 1102 70-71 0.5258320509311147 1102 72-73 0.5293704319429366 1102 74-75 0.5121208672955646 1102 76-77 0.5093387911583989 1102 78-79 0.4291906856963621 1102 80-81 0.4189829267969998 1102 82-83 0.4528818633021885 1102 84-85 0.45871897684616414 1102 86-87 0.5224617529752393 1102 88-89 0.4920963533973186 1102 90-91 0.4935706557366686 1102 92-93 0.46869499285183025 1102 94-95 0.5130782930133044 1102 96-97 0.5383822766238033 1102 98-99 0.5628685140064711 1102 100 0.5662549223602369 1103 1 0.14762357760927358 1103 2 0.1892402343637869 1103 3 0.15389056903059384 1103 4 0.17925912365458174 1103 5 0.1300642580198712 1103 6 0.13948238801799562 1103 7 0.13197517365371425 1103 8 0.17761641400000627 1103 9 0.23971890269489649 1103 10-11 0.2255167677868215 1103 12-13 0.257649454541216 1103 14-15 0.3242221250983306 1103 16-17 0.30969356312187557 1103 18-19 0.31681027893391445 1103 20-21 0.2764966398540807 1103 22-23 0.2754087814373136 1103 24-25 0.3213106025291168 1103 26-27 0.33865266711726605 1103 28-29 0.30213257384340153 1103 30-31 0.32368196276647865 1103 32-33 0.3200698660986916 1103 34-35 0.35987839167037805 1103 36-37 0.3687688214100717 1103 38-39 0.3603230560580428 1103 40-41 0.34392100539393766 1103 42-43 0.44327722462593755 1103 44-45 0.47955451002569305 1103 46-47 0.3913551171370955 1103 48-49 0.33882636349522954 1103 50-51 0.2732475002777335 1103 52-53 0.3422345432388525 1103 54-55 0.29618697490558077 1103 56-57 0.3650419240770759 1103 58-59 0.3231542503756586 1103 60-61 0.33828738236572065 1103 62-63 0.2742863667695161 1103 64-65 0.30627760264674464 1103 66-67 0.297442502209762 1103 68-69 0.2926505785981277 1103 70-71 0.277688342298994 1103 72-73 0.28576451232566313 1103 74-75 0.27993963760356877 1103 76-77 0.3435349617697341 1103 78-79 0.37957402099473114 1103 80-81 0.33251226412067325 1103 82-83 0.315622345292347 1103 84-85 0.29282507280256453 1103 86-87 0.2823829367959476 1103 88-89 0.2956425891372483 1103 90-91 0.30443012720222384 1103 92-93 0.30041620819866566 1103 94-95 0.32706698110934695 1103 96-97 0.3578299212015459 1103 98-99 0.40652834570214935 1103 100 0.40663030420356705 1104 1 0.11506852177137716 1104 2 0.18959371265351166 1104 3 0.15086615992503027 1104 4 0.1396556390638537 1104 5 0.08289956391782027 1104 6 0.11314574471799688 1104 7 0.13966281808728098 1104 8 0.17029407519298445 1104 9 0.24269485430235704 1104 10-11 0.21733103339207105 1104 12-13 0.22087950243957977 1104 14-15 0.30995403403852606 1104 16-17 0.3012110732381288 1104 18-19 0.28062701807102997 1104 20-21 0.28704090429153695 1104 22-23 0.26383457037670155 1104 24-25 0.2662532983214092 1104 26-27 0.2765961732273752 1104 28-29 0.2600274346013478 1104 30-31 0.2622539292890522 1104 32-33 0.22387873749769227 1104 34-35 0.24015155445822245 1104 36-37 0.25408192366186455 1104 38-39 0.2959371647235187 1104 40-41 0.30107622185675353 1104 42-43 0.3187693075450042 1104 44-45 0.3847377456292591 1104 46-47 0.36723746897160936 1104 48-49 0.32411855141890555 1104 50-51 0.2693009116454874 1104 52-53 0.29301836644111035 1104 54-55 0.31442658883695884 1104 56-57 0.32545476259447526 1104 58-59 0.35425601009801255 1104 60-61 0.31677923203032776 1104 62-63 0.2971027588884212 1104 64-65 0.30410234099215927 1104 66-67 0.2866237813089896 1104 68-69 0.29541720709362096 1104 70-71 0.2884570771394266 1104 72-73 0.30294683842777204 1104 74-75 0.32275485279870963 1104 76-77 0.29835736400170987 1104 78-79 0.3201874273515628 1104 80-81 0.28442696747018203 1104 82-83 0.3071461564049969 1104 84-85 0.337478242306112 1104 86-87 0.3133849526444692 1104 88-89 0.3111971693640392 1104 90-91 0.28758416087602257 1104 92-93 0.3150838557458009 1104 94-95 0.2822405860791193 1104 96-97 0.2977894141637485 1104 98-99 0.32118184747778855 1104 100 0.3593918379526855 1105 1 0.0999703375050629 1105 2 0.1175060472094529 1105 3 0.13575312566423037 1105 4 0.1171001100964375 1105 5 0.08147877557583172 1105 6 0.12669027014859324 1105 7 0.17925520146194174 1105 8 0.19966478403173227 1105 9 0.19816304022515396 1105 10-11 0.18425292599577858 1105 12-13 0.12556034434206964 1105 14-15 0.2254984062117984 1105 16-17 0.20450045049815913 1105 18-19 0.2243526145240864 1105 20-21 0.17929617792774621 1105 22-23 0.22140966814274776 1105 24-25 0.14986801194860533 1105 26-27 0.16403173668226145 1105 28-29 0.22096426874539787 1105 30-31 0.19816722585586177 1105 32-33 0.17794644798950543 1105 34-35 0.19623076725852684 1105 36-37 0.1730897378768006 1105 38-39 0.20043350384374747 1105 40-41 0.20846013904662186 1105 42-43 0.24915315154657236 1105 44-45 0.21051992171708633 1105 46-47 0.2293209807176595 1105 48-49 0.2069641630009471 1105 50-51 0.17080591759878416 1105 52-53 0.218153946890844 1105 54-55 0.2038735605279811 1105 56-57 0.26605954199196447 1105 58-59 0.2189601494244542 1105 60-61 0.20374583131520296 1105 62-63 0.22968237279985715 1105 64-65 0.2577285497246038 1105 66-67 0.20014149208437004 1105 68-69 0.20896919380651724 1105 70-71 0.21861340041087374 1105 72-73 0.22348076843433518 1105 74-75 0.2312110605412343 1105 76-77 0.26825391208622307 1105 78-79 0.25398840963367775 1105 80-81 0.225938839630885 1105 82-83 0.19228772496381907 1105 84-85 0.19604693506123283 1105 86-87 0.14997986664633345 1105 88-89 0.19452267766859777 1105 90-91 0.22270360764666464 1105 92-93 0.23871148980893153 1105 94-95 0.29909971091424836 1105 96-97 0.27377615329337246 1105 98-99 0.3146423937033198 1105 100 0.35791990112286953 1106 1 0.05398508926428747 1106 2 0.11908960604685603 1106 3 0.07421381206543032 1106 4 0.07589484932346835 1106 5 0.08245290320883925 1106 6 0.09398206554917721 1106 7 0.09070806594725411 1106 8 0.10769379339919993 1106 9 0.11298995461091721 1106 10-11 0.1303439992701243 1106 12-13 0.10050463987224134 1106 14-15 0.19900473538859842 1106 16-17 0.16988958256279574 1106 18-19 0.18882931630658817 1106 20-21 0.15442416108145807 1106 22-23 0.135706891687839 1106 24-25 0.1374357649247102 1106 26-27 0.19019116638209255 1106 28-29 0.1399522139842091 1106 30-31 0.15490898882063675 1106 32-33 0.12424535076366539 1106 34-35 0.19426282397707695 1106 36-37 0.2043624013638521 1106 38-39 0.14770143019142523 1106 40-41 0.20632873582194833 1106 42-43 0.231457500852855 1106 44-45 0.22722388941396332 1106 46-47 0.22128341343542957 1106 48-49 0.24447369458820845 1106 50-51 0.21736576590171808 1106 52-53 0.25508561441866817 1106 54-55 0.24453773247774535 1106 56-57 0.24711080243197614 1106 58-59 0.23308460348189897 1106 60-61 0.22870256254495303 1106 62-63 0.19990946404485754 1106 64-65 0.26150503358392285 1106 66-67 0.303701135990849 1106 68-69 0.2761889810058591 1106 70-71 0.2498607126521648 1106 72-73 0.22142069200270242 1106 74-75 0.2950532737633793 1106 76-77 0.2903475975466847 1106 78-79 0.19434292967302014 1106 80-81 0.15835588396978295 1106 82-83 0.08330333709191251 1106 84-85 0.11692057995294647 1106 86-87 0.15755646908628762 1106 88-89 0.1618194340849577 1106 90-91 0.21586553335512093 1106 92-93 0.2636723042862634 1106 94-95 0.29659887781470573 1106 96-97 0.2551656990924194 1106 98-99 0.29055789097252216 1106 100 0.3483648156811725 1107 1 0.05568288948991551 1107 2 0.10001904268479933 1107 3 0.05252715256029461 1107 4 0.10193344119763736 1107 5 0.05846912698411444 1107 6 0.05600331502837719 1107 7 -0.0019539849099246 1107 8 0.009705552953164442 1107 9 0.04624612198225719 1107 10-11 0.048751321403571524 1107 12-13 0.027602120645994432 1107 14-15 0.14898712336879072 1107 16-17 0.09578637127588507 1107 18-19 0.05109646202222251 1107 20-21 0.07648324242224191 1107 22-23 0.1062926268916371 1107 24-25 0.07545976577987545 1107 26-27 0.11565597931735994 1107 28-29 0.0780741534346916 1107 30-31 0.09912527831949802 1107 32-33 0.15747685852814897 1107 34-35 0.1504264599286671 1107 36-37 0.16625435875959482 1107 38-39 0.10989593864186986 1107 40-41 0.13980657563887178 1107 42-43 0.15721515688326093 1107 44-45 0.17060105623748711 1107 46-47 0.26886836363985367 1107 48-49 0.2647178473079421 1107 50-51 0.23746352233857237 1107 52-53 0.22635971153864176 1107 54-55 0.2224031741199255 1107 56-57 0.24702445401609907 1107 58-59 0.2937845207122223 1107 60-61 0.2649246776785503 1107 62-63 0.2827035350674052 1107 64-65 0.2891372088113684 1107 66-67 0.30695483727517825 1107 68-69 0.2711134740501464 1107 70-71 0.2717109168528111 1107 72-73 0.2916375550606958 1107 74-75 0.2813970737803757 1107 76-77 0.2870076236405765 1107 78-79 0.2789726577367162 1107 80-81 0.32596982516059114 1107 82-83 0.3470710933415617 1107 84-85 0.2756704971794832 1107 86-87 0.2634313939838222 1107 88-89 0.2949401548928101 1107 90-91 0.31241685051364243 1107 92-93 0.3356392528589396 1107 94-95 0.3558864732555662 1107 96-97 0.31010915632306535 1107 98-99 0.3677964573899857 1107 100 0.42697863426241156 1108 1 -0.026496973791310552 1108 2 0.07244349849871412 1108 3 0.08494968880249587 1108 4 0.06294254505308317 1108 5 0.0017655519214372362 1108 6 0.013889881274693039 1108 7 0.014527507758444358 1108 8 0.02661637708879283 1108 9 0.07639846336735445 1108 10-11 0.07536428709629206 1108 12-13 0.09117213305560057 1108 14-15 0.12120235174565153 1108 16-17 0.05972377736421919 1108 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-0.22662831779921078 2215 4 -0.17800026285919301 2215 5 -0.18529112268620906 2215 6 -0.22638162765377956 2215 7 -0.2428228491675526 2215 8 -0.22024956804987283 2215 9 -0.23889524049844368 2215 10-11 -0.3155344914877247 2215 12-13 -0.31314558494941735 2215 14-15 -0.4271357512538003 2215 16-17 -0.3943244780797883 2215 18-19 -0.3855560768832831 2215 20-21 -0.4475833752852054 2215 22-23 -0.4024062412074585 2215 24-25 -0.4234680628664975 2215 26-27 -0.3946624435931625 2215 28-29 -0.4001162997602208 2215 30-31 -0.3916639696832718 2215 32-33 -0.4196941050503611 2215 34-35 -0.38348124130304484 2215 36-37 -0.36109751474241847 2215 38-39 -0.4203314643093208 2215 40-41 -0.4937920938406677 2215 42-43 -0.41468285773304814 2215 44-45 -0.43507680259056514 2215 46-47 -0.4329670797195888 2215 48-49 -0.3875946250472211 2215 50-51 -0.36952339224807673 2215 52-53 -0.377028724143905 2215 54-55 -0.3510199866653565 2215 56-57 -0.34869969076792984 2215 58-59 -0.3654300710111471 2215 60-61 -0.4058723969406799 2215 62-63 -0.36422625550754617 2215 64-65 -0.3087299980476814 2215 66-67 -0.38302944003388006 2215 68-69 -0.46987427194899567 2215 70-71 -0.3458151452069771 2215 72-73 -0.3430307462136426 2215 74-75 -0.43595751596431853 2215 76-77 -0.4150530156414902 2215 78-79 -0.36905151990529106 2215 80-81 -0.4100944868010714 2215 82-83 -0.38669164032118175 2215 84-85 -0.3994529004185807 2215 86-87 -0.3680447578005257 2215 88-89 -0.3918876352781595 2215 90-91 -0.41541512333089514 2215 92-93 -0.37899637015366494 2215 94-95 -0.4633967290994754 2215 96-97 -0.48456568833081803 2215 98-99 -0.5266299901921947 2215 100 -0.5495928284674783 2216 1 -0.21266409193000868 2216 2 -0.27530547287991425 2216 3 -0.2678769761960993 2216 4 -0.1449118859205143 2216 5 -0.27148659272964437 2216 6 -0.33449675779763766 2216 7 -0.30327142432139453 2216 8 -0.3781105643363034 2216 9 -0.7230389956142957 2216 10-11 -0.5594829142220945 2216 12-13 -0.5224845526590869 2216 14-15 -0.7195383141241081 2216 16-17 -0.5065628950440626 2216 18-19 -0.5088041634536395 2216 20-21 -0.6994728286155905 2216 22-23 -0.6582767475833506 2216 24-25 -0.5602736633267753 2216 26-27 -0.6468285726422565 2216 28-29 -0.5987462123407568 2216 30-31 -0.604038766222601 2216 32-33 -0.5849507882447256 2216 34-35 -0.6898497602175127 2216 36-37 -0.7111250339878694 2216 38-39 -0.8062239184897848 2216 40-41 -0.8965732199153535 2216 42-43 -0.9970885501031503 2216 44-45 -0.9116326754269792 2216 46-47 -1.0153103529510474 2216 48-49 -0.7701217685949828 2216 50-51 -0.8252280340089726 2216 52-53 -0.8964109134223932 2216 54-55 -0.7413836376192648 2216 56-57 -1.0520489934121855 2216 58-59 -1.074275017852841 2216 60-61 -0.8993641808208963 2216 62-63 -1.1059002802913511 2216 64-65 -1.1914949520360736 2216 66-67 -1.0210443139072254 2216 68-69 -0.8824090995565115 2216 70-71 -1.0003055643338925 2216 72-73 -0.8527483612419076 2216 74-75 -0.8515999792884443 2216 76-77 -0.834155784223654 2216 78-79 -0.7904789766058045 2216 80-81 -0.839137679230312 2216 82-83 -0.8760860851173575 2216 84-85 -0.8858424677913312 2216 86-87 -0.8216646716780787 2216 88-89 -0.8034978823283971 2216 90-91 -0.8198539473930069 2216 92-93 -0.8195494583586225 2216 94-95 -0.9013653420266152 2216 96-97 -1.1754093648975044 2216 98-99 -1.211424211124367 2216 100 -1.2514079758923025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 355.0 3 950.0 4 572.0 5 486.0 6 701.0 7 1424.0 8 3116.0 9 5860.0 10 7281.0 11 8683.0 12 11495.0 13 16042.0 14 19729.0 15 26824.0 16 36166.0 17 52624.0 18 58759.0 19 31981.0 20 20285.0 21 18575.0 22 18704.0 23 19665.0 24 22392.0 25 27175.0 26 34473.0 27 44047.0 28 56619.0 29 71740.0 30 90671.0 31 114449.0 32 144402.0 33 182750.0 34 243735.0 35 367299.0 36 596070.0 37 1151196.0 38 1623409.0 39 789045.0 40 5054.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.43060271066302 21.351444271866036 15.23766005296232 23.980292964508624 2 14.089700535190792 21.538471405715935 39.2603095832891 25.111518475804175 3 16.463244616857438 27.139602537945635 31.27331727426043 25.1238355709365 4 11.256007519374805 18.481059265746225 38.24441514988502 32.01851806499395 5 11.854707394209948 38.7877305600552 34.93995568601855 14.417606359716306 6 28.698017469947946 39.17814651390097 17.5617653447718 14.562070671379285 7 25.287355545829964 34.43414067944538 22.110608572133117 18.16789520259155 8 25.593374159444625 34.99966159212382 19.814329691637006 19.592634556794547 9 25.70504031948404 16.07528554113951 21.732536929919863 36.487137209456584 10-11 23.676151629542066 27.491965512683763 28.006596390468907 20.82528646730526 12-13 24.961167485231154 25.599560019126372 28.47540247329742 20.963870022345045 14-15 22.082354468157 26.816925389755575 27.352310279346 23.74840986274143 16-17 19.900720749702565 29.996777951942033 28.263235081402705 21.839266216952698 18-19 19.7949872763702 27.501808920904207 32.33463627398244 20.36856752874315 20-21 21.288007381848814 26.498214371009464 32.192538722384526 20.021239524757195 22-23 20.877639307163463 26.6416874957699 32.27718288692468 20.20349031014196 24-25 20.520337190949157 26.672091346614344 32.32893206803602 20.478639394400478 26-27 20.26715487417894 26.839744713874875 32.374966728851575 20.51813368309461 28-29 20.21145241653937 26.711039025976387 32.73103013282593 20.346478424658315 30-31 20.60973098515 26.994521130611794 32.179445631589076 20.216302252649132 32-33 20.180738880134243 26.88154121230109 32.48916565066731 20.448554256897353 34-35 20.58989087391003 26.665770419663016 32.32045606988769 20.423882636539265 36-37 20.509752235797247 26.829718876383236 32.00344092802096 20.657087959798563 38-39 20.495065723264787 26.898135501061354 31.993516664940845 20.613282110733007 40-41 20.549019065086586 26.683458429037405 31.99594479445099 20.771577711425017 42-43 20.528903800503926 26.89379592646904 31.912541217360307 20.664759055666728 44-45 20.74079437591332 26.89154000420605 31.488714728357163 20.878950891523463 46-47 20.87543761162873 27.00580646620445 30.937720156076836 21.18103576608998 48-49 20.9321818056305 27.18106045351657 30.338745810594748 21.548011930258184 50-51 21.058374254467665 27.305178422672032 30.00732778543826 21.629119537422046 52-53 21.114826974104727 27.54753830077865 29.801380615448856 21.53625410966777 54-55 21.148125515476597 27.735575706291023 29.724061099436422 21.392237678795954 56-57 21.17057606437872 27.395035684373315 29.751122552976295 21.68326569827167 58-59 21.195391088375224 27.014588483763884 30.08888702086437 21.70113340699652 60-61 21.06079273323786 27.36884443416362 29.907010944550294 21.663351888048222 62-63 20.8937539105679 27.196648376419137 30.13475392896561 21.774843784047366 64-65 20.99075317292672 27.1803549019492 30.038752788099384 21.790139137024696 66-67 20.922850884651233 27.17191361554012 29.964289888633232 21.940945611175415 68-69 21.28623470475632 27.29844600417047 29.802539630940235 21.612779660132983 70-71 21.23235799472723 27.250783995355103 29.683996705362897 21.83286130455477 72-73 21.176425612800966 27.18904746706346 29.80926960778274 21.825257312352832 74-75 21.224535918268177 27.243996687478365 29.499881978969217 22.031585415284233 76-77 21.300936081756642 27.140666111598982 29.33984471719988 22.21855308944449 78-79 21.309143023128836 27.15876348934448 29.604512851049552 21.927580636477135 80-81 20.953219879209488 27.2965531512187 29.60511092098758 22.14511604858423 82-83 21.01417597832547 27.293387170848483 29.80386821972471 21.888568631101343 84-85 21.027533558264707 27.023757884272303 29.604060579566806 22.344647977896187 86-87 21.106988704940907 27.245445966726656 29.416049782583485 22.231515545748948 88-89 20.969659244366436 27.18454942721302 29.45249825184061 22.39329307657993 90-91 20.816818719542233 27.142328614132825 29.36203110888244 22.6788215574425 92-93 20.825494794242356 27.213894307743775 29.497968365439696 22.462642532574172 94-95 20.69189473472789 27.00588525896979 29.651669431034243 22.65055057526807 96-97 20.714885857326635 27.297655709631652 29.325942676670753 22.661515756370957 98-99 20.52816463107171 27.199031184794325 29.501207854323276 22.771596329810688 100 20.618612207917327 27.126383469557847 29.411549259735974 22.843455062788852 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4994.0 1 2924.0 2 1962.0 3 3460.0 4 2501.5 5 1673.0 6 4769.0 7 13246.5 8 22061.5 9 23669.5 10 16055.5 11 8037.5 12 6276.5 13 6420.5 14 6955.5 15 7608.5 16 8216.0 17 9056.0 18 10046.5 19 10963.5 20 12602.0 21 14837.5 22 17860.5 23 22962.5 24 29632.5 25 37148.5 26 47653.5 27 61390.5 28 77605.0 29 94086.5 30 106686.0 31 118709.5 32 132388.0 33 145217.5 34 155514.5 35 164364.0 36 172263.5 37 178649.0 38 184932.5 39 189039.0 40 193746.0 41 196431.5 42 195100.0 43 198208.5 44 199013.5 45 196344.5 46 196600.5 47 194390.5 48 190552.5 49 192400.0 50 197770.5 51 191327.0 52 176292.0 53 163252.0 54 154518.0 55 147808.0 56 130465.0 57 111000.0 58 100197.0 59 93439.5 60 79675.0 61 61907.5 62 51259.0 63 42232.0 64 33057.0 65 26168.0 66 20752.5 67 16223.0 68 12568.0 69 9180.0 70 6347.5 71 4461.0 72 3149.5 73 2211.5 74 1633.5 75 1169.5 76 746.5 77 533.0 78 347.0 79 185.0 80 91.0 81 37.5 82 20.0 83 10.5 84 8.0 85 7.5 86 3.0 87 1.0 88 1.5 89 2.0 90 1.5 91 1.0 92 1.0 93 0.5 94 1.5 95 2.0 96 3.5 97 3.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.048221012580502674 2 0.0 3 1.856601814440075E-4 4 0.06916685668704935 5 0.004422087958029996 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0110383416967619 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.979068502362019E-4 26-27 0.0 28-29 3.1224666879219444E-4 30-31 0.003763838223819425 32-33 2.109774789136449E-4 34-35 0.0016371852363698844 36-37 0.004025450297672345 38-39 0.0012574257743253236 40-41 0.0012236693776991403 42-43 0.0019409928060055328 44-45 1.6878198313091592E-5 46-47 4.894677510796562E-4 48-49 6.076151392712973E-4 50-51 0.0015781115422740636 52-53 9.114227089069459E-4 54-55 0.0011392783861336824 56-57 0.006093029591026065 58-59 0.00913110528738255 60-61 0.001240547576012232 62-63 0.0025654861435899217 64-65 0.0011477174852902283 66-67 1.772210822874617E-4 68-69 7.510798249325758E-4 70-71 3.881985612011066E-4 72-73 0.0 74-75 1.940992806005533E-4 76-77 0.0 78-79 5.738587426451141E-4 80-81 0.0 82-83 0.0010464482954116788 84-85 5.569805443320225E-4 86-87 0.0 88-89 0.0 90-91 0.0 92-93 2.8692937132255707E-4 94-95 0.0 96-97 4.219549578272898E-4 98-99 6.835670316802094E-4 100 4.219549578272898E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5924803.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.63888577753548 #Duplication Level Percentage of deduplicated Percentage of total 1 65.26478855275286 29.1334744150134 2 17.765425657566343 15.860576134348046 3 7.178990242684563 9.61386376323714 4 3.429398870428171 6.123381778506093 5 1.9228276709954792 4.291644238772588 6 1.1266430146396893 3.0175253305535596 7 0.7367048738498106 2.301997930087477 8 0.5171869995414652 1.846932111852621 9 0.35219025826071576 1.4149242639414734 >10 1.541274927119962 11.80782262922217 >50 0.09660421550852918 3.024172667161792 >100 0.058889143838366416 5.097540678357368 >500 0.005923816887561132 1.861755560281689 >1k 0.002923918148587338 2.341028320426744 >5k 1.1391888889540164E-4 0.42785280963180794 >10k+ 1.1391888889540164E-4 1.8355073686058525 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43656 0.7368346255563265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37868 0.6391436137201524 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26761 0.451677465056644 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9495 0.16025849298280465 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8402 0.14181062222659555 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7344 0.12395348841134465 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.6878198313091592E-5 0.0 9 0.0 0.0 0.0 1.6878198313091592E-5 0.0 10-11 0.0 0.0 0.0 3.3756396626183184E-5 0.0 12-13 0.0 0.0 0.0 3.3756396626183184E-5 0.0 14-15 0.0 0.0 0.0 3.3756396626183184E-5 0.0 16-17 0.0 0.0 0.0 3.3756396626183184E-5 0.0 18-19 0.0 0.0 0.0 4.219549578272898E-5 0.0 20-21 0.0 0.0 0.0 6.751279325236637E-5 0.0 22-23 0.0 0.0 0.0 2.7005117300946547E-4 0.0 24-25 0.0 0.0 0.0 8.101535190283963E-4 0.0 26-27 0.0 0.0 0.0 0.0018312845169704377 0.0 28-29 0.0 0.0 0.0 0.004430527057186543 0.0 30-31 0.0 0.0 0.0 0.011468735753745737 0.0 32-33 0.0 0.0 0.0 0.024296166471695347 0.0 34-35 0.0 0.0 0.0 0.04538547526390329 0.0 36-37 0.0 0.0 0.0 0.0799942209048976 0.0 38-39 0.0 0.0 0.0 0.12368343723833519 0.0 40-41 0.0 0.0 0.0 0.16873134853597666 0.0 42-43 8.439099156545796E-6 0.0 0.0 0.21434467947710667 0.0 44-45 1.6878198313091592E-5 0.0 0.0 0.2723972425749852 0.0 46-47 1.6878198313091592E-5 0.0 0.0 0.34601150451753415 0.0 48-49 1.6878198313091592E-5 0.0 0.0 0.422545694768248 0.0 50-51 1.6878198313091592E-5 0.0 0.0 0.5012993680971334 0.0 52-53 1.6878198313091592E-5 0.0 0.0 0.5780445358267609 0.0 54-55 1.6878198313091592E-5 0.0 0.0 0.6655411158818276 0.0 56-57 1.6878198313091592E-5 0.0 0.0 0.7719497171467136 0.0 58-59 1.6878198313091592E-5 0.0 0.0 0.8913798484101496 0.0 60-61 1.6878198313091592E-5 0.0 0.0 1.0183629734186943 0.0 62-63 1.6878198313091592E-5 0.0 0.0 1.1445528231065234 0.0 64-65 1.6878198313091592E-5 0.0 0.0 1.2786247914065667 0.0 66-67 1.6878198313091592E-5 0.0 0.0 1.434967542380734 0.0 68-69 1.6878198313091592E-5 0.0 0.0 1.619412493546199 0.0 70-71 1.6878198313091592E-5 0.0 0.0 1.8160181865962461 0.0 72-73 1.6878198313091592E-5 0.0 0.0 2.019358280773217 0.0 74-75 1.6878198313091592E-5 0.0 0.0 2.2259474281254583 0.0 76-77 1.6878198313091592E-5 0.0 0.0 2.450182731814037 0.0 78-79 1.6878198313091592E-5 0.0 0.0 2.7087651690697565 0.0 80-81 1.6878198313091592E-5 0.0 0.0 2.991787237482833 0.0 82-83 1.6878198313091592E-5 0.0 0.0 3.290919546185755 0.0 84-85 1.6878198313091592E-5 0.0 0.0 3.5891657494772398 0.0 86-87 1.6878198313091592E-5 0.0 0.0 3.9125098336602924 0.0 88 1.6878198313091592E-5 0.0 0.0 4.169488842076269 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17000 0.0 62.54824 1 GTATCAA 32415 0.0 42.905598 1 GTGGTAT 5705 0.0 36.86442 1 TGGTATC 6090 0.0 33.667873 2 ATCAACG 42960 0.0 32.150383 3 TCAACGC 43515 0.0 31.77275 4 TATCAAC 43740 0.0 31.695324 2 CAACGCA 46245 0.0 29.844324 5 AACGCAG 47835 0.0 28.881071 6 ACGCAGA 52845 0.0 26.045137 7 CGCAGAG 52920 0.0 25.892752 8 GCAGAGT 56135 0.0 24.200459 9 GTACATG 29615 0.0 23.719397 1 TACATGG 29360 0.0 23.225145 2 GAGTACT 38820 0.0 22.341467 12-13 CATGGGT 7425 0.0 22.29424 4 ACATGGG 30175 0.0 22.208237 3 CAGAGTA 55360 0.0 21.45698 10-11 AGTACTT 38955 0.0 21.43766 12-13 AGAGTAC 53980 0.0 21.208733 10-11 >>END_MODULE