FastQCFastQC Report
Wed 25 May 2016
SRR2431450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4457863
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA423840.9507694606137514No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65230.14632571705321587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG43450.041.8667832
GGTATCA77650.040.076451
GTACCTG51300.036.561861
GTATCAA117500.029.965171
ACCTGGG62750.028.4654623
TCAACGC127850.025.9198154
ATCAACG128300.025.8291933
GTACATG199600.024.9642241
TATCAAC137500.024.9214462
TACATGG199200.024.0690652
CAACGCA139950.023.9474985
CATGGGT81900.023.8755254
TCTGTCG11450.023.8079598
GTCTCGC12300.022.9307841
GTACACG4750.022.7618221
AACGCAG146100.022.7453826
ACATGGG207800.022.1002623
TATAACG3550.021.1855642
ACGCAGA157850.020.6647037
TATACCG2751.4370016E-920.5112485
Produced by FastQC (version 0.11.5)