##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431450_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4457863 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.02337891496441 31.0 31.0 34.0 26.0 34.0 2 29.955102927120013 31.0 30.0 34.0 25.0 34.0 3 30.07981223290173 31.0 31.0 34.0 25.0 34.0 4 33.32110497787841 37.0 35.0 37.0 26.0 37.0 5 33.43673818598732 37.0 35.0 37.0 27.0 37.0 6 33.446362079767816 37.0 35.0 37.0 27.0 37.0 7 33.270675657820796 37.0 35.0 37.0 26.0 37.0 8 33.20254480678298 37.0 35.0 37.0 26.0 37.0 9 34.49161963927559 39.0 35.0 39.0 23.0 39.0 10-11 34.464327189956265 39.0 35.0 39.0 24.5 39.0 12-13 34.44272020023944 39.0 35.0 39.0 24.0 39.0 14-15 35.63110889679652 40.0 36.0 41.0 24.0 41.0 16-17 35.65421840016169 40.0 36.0 41.0 25.0 41.0 18-19 35.65279619405082 40.0 36.0 41.0 25.0 41.0 20-21 35.75897778823621 40.0 36.0 41.0 25.0 41.0 22-23 35.73926587694597 40.0 36.0 41.0 25.0 41.0 24-25 35.79359482334921 40.0 36.0 41.0 26.0 41.0 26-27 35.79976717543809 40.0 36.0 41.0 27.0 41.0 28-29 35.6937107533363 40.0 35.5 41.0 26.5 41.0 30-31 35.68243965774632 40.0 35.0 41.0 27.0 41.0 32-33 35.624007287796864 40.0 35.0 41.0 26.5 41.0 34-35 35.52121678032726 40.0 35.0 41.0 25.5 41.0 36-37 35.39618344036145 40.0 35.0 41.0 25.0 41.0 38-39 35.2882695138904 40.0 35.0 41.0 25.0 41.0 40-41 35.199724397093405 40.0 35.0 41.0 25.0 41.0 42-43 34.98859621751498 40.0 35.0 41.0 24.0 41.0 44-45 34.87074524273177 39.0 34.5 41.0 24.0 41.0 46-47 34.74458311078649 39.0 34.0 41.0 24.0 41.0 48-49 34.62148915298653 39.0 34.0 41.0 23.5 41.0 50-51 34.471240816507816 39.0 34.0 41.0 23.0 41.0 52-53 34.3841149896262 39.0 34.0 41.0 23.0 41.0 54-55 34.31837452160374 39.0 33.5 41.0 23.0 41.0 56-57 34.172243068035066 38.5 33.0 41.0 22.0 41.0 58-59 33.979351765633 38.0 33.0 40.5 21.5 41.0 60-61 33.77604796289164 38.0 33.0 40.0 20.5 41.0 62-63 33.506899269896806 37.5 33.0 40.0 19.0 41.0 64-65 33.3657841212258 37.0 33.0 40.0 18.0 41.0 66-67 33.121296235438365 37.0 32.5 40.0 16.5 41.0 68-69 32.8573282759026 36.0 32.0 40.0 13.5 41.0 70-71 32.552789307343005 36.0 32.0 39.0 11.5 41.0 72-73 32.23758334430646 35.5 32.0 39.0 8.0 41.0 74-75 31.90036391876556 35.0 31.5 38.5 6.5 40.5 76-77 31.570931744649847 35.0 31.0 37.5 2.0 40.0 78-79 31.222823581612985 35.0 31.0 37.0 2.0 39.0 80-81 30.905129767334707 35.0 31.0 36.5 2.0 39.0 82-83 30.59112830519915 35.0 31.0 36.0 2.0 38.5 84-85 30.28413513380739 35.0 31.0 36.0 2.0 37.0 86-87 30.008657960103307 35.0 30.5 35.5 2.0 37.0 88-89 29.72682134915317 35.0 30.0 35.0 2.0 36.5 90-91 29.49356934028704 35.0 30.0 35.0 2.0 36.0 92-93 29.286976069026796 34.5 30.0 35.0 2.0 36.0 94-95 29.05053699496822 34.0 30.0 35.0 2.0 36.0 96-97 28.942330439495336 34.0 30.0 35.0 2.0 35.5 98-99 28.781816646227128 34.0 30.0 35.0 2.0 35.0 100 28.64524683688126 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3868416713956364 1101 2 0.5247150361887165 1101 3 0.5122702895834301 1101 4 0.5260949940240209 1101 5 0.32171983200890963 1101 6 0.39863253457170345 1101 7 0.3678939498813918 1101 8 0.412851953081649 1101 9 0.5152225867060025 1101 10-11 0.5437982182221646 1101 12-13 0.5640373473110429 1101 14-15 0.678333585461445 1101 16-17 0.5895889830588104 1101 18-19 0.5193463172692816 1101 20-21 0.5129931490373991 1101 22-23 0.5292199595360358 1101 24-25 0.5576216088047445 1101 26-27 0.6227788770717808 1101 28-29 0.6574374161391603 1101 30-31 0.697415586495822 1101 32-33 0.6722768433625319 1101 34-35 0.6971512571850695 1101 36-37 0.6745770691901001 1101 38-39 0.7509037749272522 1101 40-41 0.6910869237080917 1101 42-43 0.6384993180089964 1101 44-45 0.6138820009725379 1101 46-47 0.580347793739385 1101 48-49 0.6107944749639813 1101 50-51 0.6529181026859661 1101 52-53 0.5580835296131923 1101 54-55 0.601219304760555 1101 56-57 0.6415053482539435 1101 58-59 0.6051214633970758 1101 60-61 0.62360587436347 1101 62-63 0.6380535884954597 1101 64-65 0.5372419429821917 1101 66-67 0.6141772159666559 1101 68-69 0.5397479679524793 1101 70-71 0.5707409400589327 1101 72-73 0.5357133628338673 1101 74-75 0.5248364883774919 1101 76-77 0.5519168679632678 1101 78-79 0.49507192118723964 1101 80-81 0.5186345184495664 1101 82-83 0.5048738905119663 1101 84-85 0.4865604768279077 1101 86-87 0.49945982845881787 1101 88-89 0.5366195048205391 1101 90-91 0.5234543388359754 1101 92-93 0.5414033984091162 1101 94-95 0.502846542290385 1101 96-97 0.5635156177783145 1101 98-99 0.526794929301051 1101 100 0.5115124240404221 1102 1 0.3061531864212874 1102 2 0.3539576728245315 1102 3 0.3266131365673637 1102 4 0.3374960352236158 1102 5 0.1968539938337983 1102 6 0.3098873389888581 1102 7 0.3391553148943771 1102 8 0.348544111675011 1102 9 0.5320631012958543 1102 10-11 0.5067494558463181 1102 12-13 0.4510298647705753 1102 14-15 0.5439956707240228 1102 16-17 0.5203309203697231 1102 18-19 0.40083875055809415 1102 20-21 0.39231743913215666 1102 22-23 0.43572871565020677 1102 24-25 0.45187019245548754 1102 26-27 0.5440247059015917 1102 28-29 0.6079737881139309 1102 30-31 0.5646762860095151 1102 32-33 0.6165958708210582 1102 34-35 0.6198156772016574 1102 36-37 0.5701948822304175 1102 38-39 0.543484205157597 1102 40-41 0.5567738275316501 1102 42-43 0.5715653109952896 1102 44-45 0.4308204131824809 1102 46-47 0.449617970718144 1102 48-49 0.48213489751670124 1102 50-51 0.4502324275975056 1102 52-53 0.3991947482320697 1102 54-55 0.48961039336320766 1102 56-57 0.4267362198692197 1102 58-59 0.4867356688963227 1102 60-61 0.49626380458202135 1102 62-63 0.4488533990720214 1102 64-65 0.48439661886200724 1102 66-67 0.5242039932666742 1102 68-69 0.46766373588044985 1102 70-71 0.5175833976549882 1102 72-73 0.48848533257017124 1102 74-75 0.47747791484296087 1102 76-77 0.4573462330014273 1102 78-79 0.4451019709187598 1102 80-81 0.45428742359782603 1102 82-83 0.38590287355668096 1102 84-85 0.4051028917879549 1102 86-87 0.34629528493940853 1102 88-89 0.3936040221290291 1102 90-91 0.3996437418217589 1102 92-93 0.43721362412822273 1102 94-95 0.4882918918977701 1102 96-97 0.43252856276213336 1102 98-99 0.4318623209371566 1102 100 0.4454386495896152 1103 1 0.3183986872625475 1103 2 0.3605372899948307 1103 3 0.3982611278665047 1103 4 0.36675983526736644 1103 5 0.33509086332292526 1103 6 0.37665080682162966 1103 7 0.34211015260165567 1103 8 0.38284535445069423 1103 9 0.5032722674565377 1103 10-11 0.5160331169952173 1103 12-13 0.4131426856608158 1103 14-15 0.5710296196187272 1103 16-17 0.4592126369089229 1103 18-19 0.41201391875905813 1103 20-21 0.4090428815860605 1103 22-23 0.35878201046751457 1103 24-25 0.4318399663680381 1103 26-27 0.44705196560062177 1103 28-29 0.4649263504315968 1103 30-31 0.42206026260550544 1103 32-33 0.5460668682921721 1103 34-35 0.4962518507854625 1103 36-37 0.5323771607683696 1103 38-39 0.5450740646476859 1103 40-41 0.5509899834111707 1103 42-43 0.5595178078929521 1103 44-45 0.4593824814359948 1103 46-47 0.43820279286772035 1103 48-49 0.4295948124297979 1103 50-51 0.3733646352978539 1103 52-53 0.3980470359901531 1103 54-55 0.486333106576204 1103 56-57 0.445894213274066 1103 58-59 0.537817858912824 1103 60-61 0.5413813254908675 1103 62-63 0.4158505901004901 1103 64-65 0.48795015184902013 1103 66-67 0.5225165659697666 1103 68-69 0.4796147955823429 1103 70-71 0.5421350661718165 1103 72-73 0.5155960477539523 1103 74-75 0.4026382449686814 1103 76-77 0.4440627517575457 1103 78-79 0.40411240132107196 1103 80-81 0.3588013529483085 1103 82-83 0.3349033274079787 1103 84-85 0.3023257613956609 1103 86-87 0.30820627488721897 1103 88-89 0.332452926446134 1103 90-91 0.37410591781384284 1103 92-93 0.3193173168831649 1103 94-95 0.286878513097534 1103 96-97 0.2664530782963155 1103 98-99 0.29298172880432105 1103 100 0.28395426963839654 1104 1 0.34058129166021445 1104 2 0.3751532031641496 1104 3 0.3464583504289074 1104 4 0.44438069337163455 1104 5 0.28142525518736505 1104 6 0.3866557269893178 1104 7 0.327352194798209 1104 8 0.41674103671476814 1104 9 0.40912163807937674 1104 10-11 0.5194236617172976 1104 12-13 0.43069849936364335 1104 14-15 0.5205216411840539 1104 16-17 0.4875230820646692 1104 18-19 0.42897927073460096 1104 20-21 0.4255437250923251 1104 22-23 0.45037118645102225 1104 24-25 0.4695430517713177 1104 26-27 0.5714139761890635 1104 28-29 0.5998202462225279 1104 30-31 0.6070227749609955 1104 32-33 0.5836777127881447 1104 34-35 0.5244261616756489 1104 36-37 0.5267801763436424 1104 38-39 0.5643922572564932 1104 40-41 0.5517117581095832 1104 42-43 0.49467456701981405 1104 44-45 0.48520089249593923 1104 46-47 0.47455365202238653 1104 48-49 0.4768556490599707 1104 50-51 0.4448927722253515 1104 52-53 0.48289003408542186 1104 54-55 0.43466375907404853 1104 56-57 0.47927165533311467 1104 58-59 0.502911858491295 1104 60-61 0.49175220540108455 1104 62-63 0.47086137609483103 1104 64-65 0.4576972512163451 1104 66-67 0.4354621722727501 1104 68-69 0.4394756349143947 1104 70-71 0.389699588062399 1104 72-73 0.44959283280713436 1104 74-75 0.47521912499734853 1104 76-77 0.3842184712468999 1104 78-79 0.43915252917495096 1104 80-81 0.47891065144849776 1104 82-83 0.3881507555533119 1104 84-85 0.42923105374769577 1104 86-87 0.42278507143143074 1104 88-89 0.44709761809327375 1104 90-91 0.42292993067950135 1104 92-93 0.3954803819694632 1104 94-95 0.4057553957395932 1104 96-97 0.4482204062487938 1104 98-99 0.5127574867289404 1104 100 0.4890641026414819 1105 1 0.1952957737007388 1105 2 0.29216521301112763 1105 3 0.3034365228116904 1105 4 0.17868918255423694 1105 5 0.2671532207470477 1105 6 0.35286427074344573 1105 7 0.30796640162754585 1105 8 0.28168913280091346 1105 9 0.33852212118411273 1105 10-11 0.4498155842243179 1105 12-13 0.3582416153338812 1105 14-15 0.49871213649472423 1105 16-17 0.5169086568925181 1105 18-19 0.444369071488282 1105 20-21 0.43135802396591316 1105 22-23 0.4293628155563525 1105 24-25 0.4253585132494777 1105 26-27 0.383813728128942 1105 28-29 0.543383663253465 1105 30-31 0.5044998025327274 1105 32-33 0.48095911796306723 1105 34-35 0.4485759255605899 1105 36-37 0.4482089138585863 1105 38-39 0.40465850646305057 1105 40-41 0.3567072076469415 1105 42-43 0.3557083720161458 1105 44-45 0.2904165634414113 1105 46-47 0.24165563612050533 1105 48-49 0.22058463799523764 1105 50-51 0.23210515696977296 1105 52-53 0.3214100370514785 1105 54-55 0.29168101340187746 1105 56-57 0.32925341520168416 1105 58-59 0.3466827579034586 1105 60-61 0.34876878626895547 1105 62-63 0.32575436047098094 1105 64-65 0.3611034203080976 1105 66-67 0.3680680057971344 1105 68-69 0.34027193397541566 1105 70-71 0.33894994914327725 1105 72-73 0.3554942774000267 1105 74-75 0.3589982250251502 1105 76-77 0.2963968108178925 1105 78-79 0.35768024928328757 1105 80-81 0.37139496585703924 1105 82-83 0.3704795560048595 1105 84-85 0.43272719709060325 1105 86-87 0.3903647087810391 1105 88-89 0.4304233519625953 1105 90-91 0.36975991209626 1105 92-93 0.3386143442151557 1105 94-95 0.4060559361096878 1105 96-97 0.5110606010049068 1105 98-99 0.5075983226189393 1105 100 0.5556653940683063 1106 1 0.24499891887442615 1106 2 0.27876189916997873 1106 3 0.25828629373483025 1106 4 0.2931966759020028 1106 5 0.19684423496308057 1106 6 0.26847331999385915 1106 7 0.2402544168349081 1106 8 0.3226138917378023 1106 9 0.4476861772042966 1106 10-11 0.4904898509029252 1106 12-13 0.32400753958942374 1106 14-15 0.38981779109055736 1106 16-17 0.3989573507987103 1106 18-19 0.37261427122183477 1106 20-21 0.3862259812347446 1106 22-23 0.4159886617890294 1106 24-25 0.4247208559370179 1106 26-27 0.4465038849493652 1106 28-29 0.5731885611459617 1106 30-31 0.5553189770047311 1106 32-33 0.4639694934452905 1106 34-35 0.4021127936865554 1106 36-37 0.44636137148520305 1106 38-39 0.4190124983384109 1106 40-41 0.5180072855156936 1106 42-43 0.39166630922311896 1106 44-45 0.41415684939865116 1106 46-47 0.3726369199627655 1106 48-49 0.37430881860174736 1106 50-51 0.45179192434154913 1106 52-53 0.5344155469408847 1106 54-55 0.4558893700300146 1106 56-57 0.43571674155264617 1106 58-59 0.4717702094579366 1106 60-61 0.5049402802522494 1106 62-63 0.5411563514102795 1106 64-65 0.5708777234006845 1106 66-67 0.49760999976642495 1106 68-69 0.5472895379452467 1106 70-71 0.548130914107567 1106 72-73 0.5413033342552183 1106 74-75 0.5175067217638336 1106 76-77 0.5592456475178516 1106 78-79 0.5404382771788896 1106 80-81 0.42100259258204176 1106 82-83 0.36442431703238043 1106 84-85 0.4552948811422688 1106 86-87 0.4648650165729009 1106 88-89 0.48269399404976454 1106 90-91 0.42179400555463076 1106 92-93 0.3821854572529979 1106 94-95 0.4193332412794568 1106 96-97 0.538023062506845 1106 98-99 0.548824601076614 1106 100 0.5813044950509614 1107 1 0.13576204454930973 1107 2 0.07647610960507834 1107 3 0.1739446539054974 1107 4 0.17371305531607817 1107 5 0.1467114873174893 1107 6 0.21612459885815127 1107 7 0.23413374152615063 1107 8 0.22808922702343892 1107 9 0.2859089477604897 1107 10-11 0.28299282520106317 1107 12-13 0.29171728057858815 1107 14-15 0.3637978899741441 1107 16-17 0.3486856489381367 1107 18-19 0.4193331595845322 1107 20-21 0.28875410220727105 1107 22-23 0.36240429860296786 1107 24-25 0.32624027513815435 1107 26-27 0.3656277549633131 1107 28-29 0.419486461083622 1107 30-31 0.34695422860056624 1107 32-33 0.331650143657491 1107 34-35 0.3193028944634335 1107 36-37 0.23964309796217975 1107 38-39 0.274690171366224 1107 40-41 0.2705065931498325 1107 42-43 0.3253428169194237 1107 44-45 0.2772980228089921 1107 46-47 0.22633478908684168 1107 48-49 0.2411042383951738 1107 50-51 0.22846263146848145 1107 52-53 0.22094179627698907 1107 54-55 0.27562287327508983 1107 56-57 0.24775612360028987 1107 58-59 0.28881448270289667 1107 60-61 0.2550123807204514 1107 62-63 0.2543185721163326 1107 64-65 0.3050302866042074 1107 66-67 0.3188696277239771 1107 68-69 0.31747970508364176 1107 70-71 0.3093521297995423 1107 72-73 0.25372149379126085 1107 74-75 0.3307644954472515 1107 76-77 0.3641084319925163 1107 78-79 0.3302288643055924 1107 80-81 0.35352260653093737 1107 82-83 0.33671581064486844 1107 84-85 0.2940708172300859 1107 86-87 0.290149763639981 1107 88-89 0.38554903387137074 1107 90-91 0.30184587672343355 1107 92-93 0.29525583012909706 1107 94-95 0.2967454410321899 1107 96-97 0.3476572422948294 1107 98-99 0.4113841826885043 1107 100 0.41068106954283934 1108 1 0.0746208851424015 1108 2 0.20352891594522404 1108 3 0.18834667299235974 1108 4 0.15156498512439498 1108 5 0.1880728813584227 1108 6 0.23050972210918275 1108 7 0.25593477900805084 1108 8 0.26363759150437716 1108 9 0.32212532273046435 1108 10-11 0.359261554849283 1108 12-13 0.25877185537828495 1108 14-15 0.30323925976438204 1108 16-17 0.25909895987414444 1108 18-19 0.2934334293877541 1108 20-21 0.35464091183081337 1108 22-23 0.27301171769378385 1108 24-25 0.28910667610056606 1108 26-27 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-0.8556249116065189 2215 44-45 -0.5291873529704887 2215 46-47 -0.4503934377815071 2215 48-49 -0.5009870122452043 2215 50-51 -0.4407567416543898 2215 52-53 -0.4414928807677043 2215 54-55 -0.41603597664060743 2215 56-57 -0.42173943711383544 2215 58-59 -0.4159816473221625 2215 60-61 -0.4367786643429028 2215 62-63 -0.4458105630475728 2215 64-65 -0.41668383932008624 2215 66-67 -0.422940951198008 2215 68-69 -0.605453757012782 2215 70-71 -0.6145216903265265 2215 72-73 -0.5504318856817534 2215 74-75 -0.4742918679140793 2215 76-77 -0.5089445811677216 2215 78-79 -0.4309663461077484 2215 80-81 -0.5085798986912451 2215 82-83 -0.5859848040077189 2215 84-85 -0.5689502027414086 2215 86-87 -0.552526290312727 2215 88-89 -0.5299512850212729 2215 90-91 -0.5997532823391438 2215 92-93 -0.5667593597072944 2215 94-95 -0.6013654135192787 2215 96-97 -0.5774287547960704 2215 98-99 -0.5694981164793731 2215 100 -0.5648668888706325 2216 1 -0.7950820816023842 2216 2 -0.8933941000535093 2216 3 -0.8554856848745302 2216 4 -0.6946448775792149 2216 5 -0.9305222777285778 2216 6 -0.9744443409353281 2216 7 -0.8416956363199688 2216 8 -0.9376965500919212 2216 9 -1.1289013201621714 2216 10-11 -1.319249142788614 2216 12-13 -1.1952595423209402 2216 14-15 -1.5006404532413384 2216 16-17 -1.4450932950170667 2216 18-19 -1.2934143924315578 2216 20-21 -1.266233156195952 2216 22-23 -1.0475790352092602 2216 24-25 -1.2238493933856844 2216 26-27 -1.3128864687246278 2216 28-29 -4.731782384445523 2216 30-31 -2.2138017962375045 2216 32-33 -1.6708305323083152 2216 34-35 -1.3917512556235607 2216 36-37 -1.4939223585926484 2216 38-39 -1.4943736974324082 2216 40-41 -1.4509203220212257 2216 42-43 -1.5928417805168706 2216 44-45 -1.3947426532865848 2216 46-47 -1.4418124046258995 2216 48-49 -1.327673523780014 2216 50-51 -1.2828212628318738 2216 52-53 -1.3532436521953954 2216 54-55 -1.631201516223534 2216 56-57 -1.3970941695892876 2216 58-59 -1.5235550387426215 2216 60-61 -1.4140027068254994 2216 62-63 -1.481772224737071 2216 64-65 -1.3857191449827724 2216 66-67 -1.4851278126795506 2216 68-69 -1.5637507858911164 2216 70-71 -1.6348862562380866 2216 72-73 -1.5758596300768986 2216 74-75 -1.5154208692964026 2216 76-77 -1.424423292853259 2216 78-79 -1.3995976681541222 2216 80-81 -1.3254438218023665 2216 82-83 -1.4155826742359139 2216 84-85 -1.3665212298384368 2216 86-87 -1.4137266176909193 2216 88-89 -1.3633473535275478 2216 90-91 -1.5232698373049587 2216 92-93 -1.48627587571551 2216 94-95 -1.3962013482800693 2216 96-97 -1.4725046314428702 2216 98-99 -1.8225161887766212 2216 100 -1.8008646199261555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 144269.0 3 30626.0 4 20580.0 5 18057.0 6 12862.0 7 11780.0 8 12886.0 9 13243.0 10 13437.0 11 13609.0 12 14542.0 13 15992.0 14 16627.0 15 17163.0 16 17018.0 17 17031.0 18 16589.0 19 15587.0 20 15169.0 21 16636.0 22 20575.0 23 30889.0 24 47549.0 25 59473.0 26 67039.0 27 55231.0 28 53392.0 29 64086.0 30 75154.0 31 85444.0 32 106227.0 33 139040.0 34 188126.0 35 285598.0 36 445398.0 37 775530.0 38 1043128.0 39 460893.0 40 1388.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15735485746901 21.60075438577598 13.408610372221085 21.833280384533925 2 15.776379289612679 23.074060148351816 38.87538608981871 22.27417447221679 3 17.476770604149376 28.885327762361435 30.069540679738438 23.568360953750748 4 12.181564957697539 20.10856494692899 37.86043239323879 29.849437702134686 5 11.725688881669823 40.10005534699786 33.37630372242774 14.79795204890458 6 27.42401885285053 40.41355479390128 18.5758191641182 13.586607189129996 7 24.79382915442751 35.737984949343186 22.132896541373153 17.33528935485615 8 23.445394383060748 37.8364820848367 20.856106845996653 17.862016686105896 9 24.179552151581536 20.351472282326444 21.407145429031964 34.061830137060056 10-11 21.99778601072402 30.32584273317862 28.05932477252993 19.617046483567428 12-13 22.90349678650905 28.49948138382745 27.74564554785935 20.85137628180415 14-15 20.991528772683257 30.34379917624238 25.508611833021668 23.156060218052694 16-17 19.895116711905665 33.21489947049116 25.953369975445423 20.93661384215775 18-19 19.812919046596715 31.776381806398035 28.042120976159985 20.368578170845268 20-21 21.912147265028057 31.02803618811575 27.077711842885716 19.982104703970478 22-23 21.307773845644775 31.36644924650735 27.100688154433744 20.225088753414134 24-25 20.880890667224218 31.101172783034464 27.40642838907556 20.611508160665757 26-27 20.702157367637287 30.791876815173858 27.614310244030253 20.8916555731586 28-29 20.713607262477645 30.882749697308892 27.67941228172614 20.724230758487327 30-31 21.123162184510864 31.514242411054195 26.988434588821526 20.37416081561342 32-33 20.392463674480272 31.143314466720955 27.713685136205275 20.750536722593495 34-35 20.584330228135624 30.442756229286232 27.839110146673118 21.133803395905023 36-37 21.174567533180028 31.03633316907975 27.143534486578186 20.645564811162036 38-39 21.0273966072379 30.968199655530388 27.421890028832923 20.58251370839879 40-41 20.612943316904424 30.719626524646728 27.729479382356836 20.937950776092013 42-43 20.48621073730801 30.75943858122076 27.737163316422276 21.017187365048954 44-45 21.006030520959662 30.802374251232372 27.537778806718684 20.65381642108928 46-47 20.730140373645494 31.26392365342757 27.293473402253582 20.712462570673353 48-49 20.493013473914445 30.72579672756334 27.72178399674719 21.059405801775025 50-51 20.57341454892761 30.450093742656637 27.76105072534888 21.215440983066873 52-53 20.5763088435014 30.598396397794925 27.622734432940295 21.202560325763383 54-55 20.82713936345301 31.098116408758603 27.358829924925328 20.71591430286306 56-57 20.431215667729916 30.7816120472268 27.390710459129018 21.396461825914262 58-59 20.346889132385716 30.27855735271866 27.821188583306476 21.553364931589144 60-61 20.87067185275441 30.84336962899328 27.26379583351981 21.022162684732503 62-63 20.661083978339544 30.7182792775392 27.434215647544512 21.186421096576737 64-65 20.62509393963187 30.371002658351376 27.769306697475127 21.23459670454163 66-67 20.515855016010477 30.45031457884392 27.390175574611696 21.64365483053391 68-69 21.127104801124258 30.738427512601525 27.294742316966243 20.839725369307978 70-71 21.16241337170207 30.356832594056588 27.49163932118523 20.98911471305612 72-73 20.746256167934725 30.46308077739981 27.966160818999438 20.824502235666028 74-75 20.727234142689827 30.37163010216614 27.740240063404343 21.160895691739693 76-77 20.821022335333975 30.410533600584156 27.33290561237938 21.43553845170249 78-79 21.079246661172057 30.590559889377978 27.442502886016158 20.887690563433807 80-81 20.663466718592076 30.350351803232016 27.727382191148237 21.258799287027667 82-83 20.52135586308029 30.406409647132694 27.87971502858878 21.192519461198238 84-85 20.71841336641437 30.194571688792976 27.73109243697479 21.355922507817862 86-87 21.08752356153367 30.329701637095457 27.460738254845364 21.12203654652551 88-89 20.723934659823794 30.17975547458846 27.645039748502505 21.45127011708524 90-91 20.67775298945992 29.965902042400362 27.590698884750026 21.765646083389694 92-93 20.887982103068868 30.24920096977229 27.41240951848439 21.450407408674447 94-95 20.78014809650298 30.02194595547683 27.640855773370042 21.557050174650144 96-97 20.734907106114978 30.009658348403356 27.65637949558768 21.59905504989399 98-99 20.668079553095687 30.05691737642805 27.627029388523766 21.647973681952497 100 20.928311670948 30.288328558478344 27.293820370785383 21.489539399788278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 35279.0 1 26740.5 2 16391.0 3 12205.5 4 8227.0 5 5793.5 6 4643.5 7 4387.5 8 4500.0 9 4462.0 10 3901.5 11 3242.0 12 2979.5 13 2993.5 14 3119.5 15 3225.0 16 3503.5 17 3996.5 18 4608.5 19 5629.5 20 7182.0 21 9246.0 22 12385.5 23 17375.0 24 23885.0 25 31755.0 26 41774.5 27 53897.0 28 68181.0 29 82558.0 30 93623.0 31 103303.5 32 112675.0 33 121181.0 34 130005.5 35 137214.5 36 141835.5 37 144657.0 38 146480.0 39 147573.0 40 147343.0 41 144466.5 42 140122.5 43 137244.5 44 134503.5 45 131707.0 46 129703.5 47 128278.0 48 129267.5 49 130990.5 50 130528.0 51 125993.5 52 118867.0 53 111828.0 54 106543.0 55 103312.5 56 95953.0 57 86410.5 58 78678.5 59 71636.0 60 62593.0 61 52157.0 62 42973.0 63 34179.5 64 26844.5 65 20908.5 66 16166.5 67 12225.0 68 9065.0 69 6764.5 70 5008.0 71 3808.5 72 3021.5 73 2436.5 74 1895.0 75 1406.0 76 974.5 77 735.0 78 554.5 79 371.0 80 271.5 81 198.0 82 145.0 83 132.5 84 100.5 85 85.5 86 79.5 87 60.0 88 48.0 89 39.5 90 34.5 91 22.5 92 20.0 93 28.5 94 24.5 95 17.0 96 16.0 97 15.5 98 18.5 99 19.0 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016308262501561848 2 0.007873727837755445 3 0.010588032875842078 4 0.009847767865454816 5 0.011889104712280302 6 0.013593957463475212 7 0.011575052889691764 8 0.008569128302058632 9 0.007357785557788564 10-11 0.006696033503048433 12-13 0.007346569421267545 14-15 0.008131698977738886 16-17 0.0054173939396522505 18-19 0.006146442813518496 20-21 0.005989416902224228 22-23 0.006460494636107031 24-25 0.005854823263971997 26-27 0.00675211418565353 28-29 0.01708217592151217 30-31 0.005854823263971997 32-33 0.005675365079635691 34-35 0.005148206663147791 36-37 0.007279272602141429 38-39 0.0064044139535019366 40-41 0.006124010540476456 42-43 0.005114558253584733 44-45 0.0045986159736178525 46-47 0.0066287366839223195 48-49 0.003936863918877723 50-51 0.004049025284087914 52-53 0.003342408683263707 54-55 0.005260368028357982 56-57 0.0031405182258853626 58-59 0.0028601148128598835 60-61 0.003757405734541416 62-63 0.003454570048473899 64-65 0.004778074157954159 66-67 0.0035218668676000138 68-69 0.003757405734541416 70-71 0.0053949616666102125 72-73 0.002938627768507018 74-75 0.00278160185721275 76-77 0.002871330949380903 78-79 0.0036115959597681673 80-81 0.0041836189223401436 82-83 0.0018506625259681602 84-85 0.0027591695841707115 86-87 0.0027030889015656158 88-89 0.001906743208573256 90-91 0.0026470082189605196 92-93 0.002590927536355424 94-95 0.0021086336659516002 96-97 0.0013795847920853557 98-99 0.0019179593450942749 100 0.0030507891337172095 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4457863.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.6453696265308 #Duplication Level Percentage of deduplicated Percentage of total 1 76.53143181041662 49.47402697432019 2 14.940106678041898 19.316174369236393 3 4.430571084012098 8.59247716147744 4 1.7021225683867653 4.401373703320895 5 0.8079492408957809 2.6115088658591366 6 0.4337809400425779 1.6825157523597867 7 0.2716605293415991 1.2293116732558684 8 0.16999621766969836 0.8791574661095869 9 0.129680681195511 0.7544930012373698 >10 0.5370124493729709 5.955721456160411 >50 0.028247585555375807 1.2436581120764558 >100 0.015910893122213925 1.9069369229031345 >500 0.001146991751664632 0.5154387750655478 >1k 3.1281561433498293E-4 0.3383154153580997 >5k 3.47572904816588E-5 0.1465651493910225 >10k+ 3.47572904816588E-5 0.9523252018686337 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42384 0.9507694606137514 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6523 0.14632571705321587 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.486454608407661E-5 0.0 5 0.0 0.0 0.0 1.1216136521019151E-4 0.0 6 0.0 0.0 0.0 1.1216136521019151E-4 0.0 7 0.0 0.0 0.0 1.1216136521019151E-4 0.0 8 0.0 0.0 0.0 1.1216136521019151E-4 0.0 9 2.2432273042038304E-5 0.0 0.0 1.1216136521019151E-4 0.0 10-11 4.486454608407661E-5 0.0 0.0 1.4580977477324897E-4 0.0 12-13 4.486454608407661E-5 0.0 0.0 1.5702591129426814E-4 0.0 14-15 6.72968191261149E-5 0.0 0.0 2.0189045737834473E-4 0.0 16-17 6.72968191261149E-5 0.0 0.0 2.691872765044597E-4 0.0 18-19 7.851295564713405E-5 0.0 0.0 3.0283568606751714E-4 0.0 20-21 8.972909216815322E-5 0.0 0.0 4.4864546084076606E-4 0.0 22-23 8.972909216815322E-5 0.0 0.0 9.870200138496854E-4 0.0 24-25 8.972909216815322E-5 1.1216136521019152E-5 0.0 0.002074985256388543 0.0 26-27 1.1216136521019151E-4 2.2432273042038304E-5 0.0 0.0036003798232471474 0.0 28-29 1.2337750173121065E-4 2.2432273042038304E-5 0.0 0.007357785557788564 0.0 30-31 1.345936382522298E-4 2.2432273042038304E-5 0.0 0.01708217592151217 0.0 32-33 1.345936382522298E-4 2.2432273042038304E-5 0.0 0.03460178116734408 0.0 34-35 1.7945818433630643E-4 2.2432273042038304E-5 0.0 0.06279914838118623 0.0 36-37 1.7945818433630643E-4 2.2432273042038304E-5 0.0 0.10589154489494182 0.0 38-39 1.7945818433630643E-4 2.2432273042038304E-5 0.0 0.16472018094768726 0.0 40-41 1.7945818433630643E-4 2.2432273042038304E-5 0.0 0.23206186461988626 0.0 42-43 1.7945818433630643E-4 2.2432273042038304E-5 0.0 0.299840977616405 0.0 44-45 1.9067432085732557E-4 2.2432273042038304E-5 0.0 0.3834011946979977 0.0 46-47 2.0189045737834473E-4 2.2432273042038304E-5 0.0 0.4827537320011853 0.0 48-49 2.0189045737834473E-4 2.2432273042038304E-5 0.0 0.5918418758046176 0.0 50-51 2.2432273042038303E-4 2.2432273042038304E-5 0.0 0.7059324164964245 0.0 52-53 2.2432273042038303E-4 2.2432273042038304E-5 0.0 0.8209763287925178 0.0 54-55 2.2432273042038303E-4 2.2432273042038304E-5 0.0 0.9511395931189451 2.2432273042038304E-5 56-57 2.355388669414022E-4 2.2432273042038304E-5 0.0 1.1037351304874106 2.2432273042038304E-5 58-59 2.4675500346242135E-4 2.2432273042038304E-5 0.0 1.2712481294288316 2.2432273042038304E-5 60-61 2.4675500346242135E-4 2.2432273042038304E-5 0.0 1.4472068791705803 2.2432273042038304E-5 62-63 2.691872765044596E-4 2.2432273042038304E-5 0.0 1.6231319805027655 2.2432273042038304E-5 64-65 2.691872765044596E-4 2.2432273042038304E-5 0.0 1.8234970433142517 2.2432273042038304E-5 66-67 2.691872765044596E-4 2.2432273042038304E-5 0.0 2.05899104570957 2.2432273042038304E-5 68-69 2.691872765044596E-4 2.2432273042038304E-5 0.0 2.316917321146926 2.2432273042038304E-5 70-71 2.691872765044596E-4 2.2432273042038304E-5 0.0 2.5995751776131297 2.2432273042038304E-5 72-73 2.691872765044596E-4 2.2432273042038304E-5 0.0 2.9124156574573963 2.2432273042038304E-5 74-75 2.691872765044596E-4 2.2432273042038304E-5 0.0 3.2295519175892125 2.2432273042038304E-5 76-77 2.691872765044596E-4 2.2432273042038304E-5 0.0 3.557478101054249 2.2432273042038304E-5 78-79 2.691872765044596E-4 2.2432273042038304E-5 0.0 3.9333532681466434 2.2432273042038304E-5 80-81 2.691872765044596E-4 4.486454608407661E-5 0.0 4.336573376077282 2.2432273042038304E-5 82-83 2.691872765044596E-4 4.486454608407661E-5 0.0 4.76488398140544 2.2432273042038304E-5 84-85 2.691872765044596E-4 4.486454608407661E-5 0.0 5.19520227517086 2.2432273042038304E-5 86-87 2.691872765044596E-4 4.486454608407661E-5 0.0 5.656925751195136 2.2432273042038304E-5 88 2.9161954954649795E-4 4.486454608407661E-5 0.0 6.026048804101876 2.2432273042038304E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4345 0.0 41.866783 2 GGTATCA 7765 0.0 40.07645 1 GTACCTG 5130 0.0 36.56186 1 GTATCAA 11750 0.0 29.96517 1 ACCTGGG 6275 0.0 28.465462 3 TCAACGC 12785 0.0 25.919815 4 ATCAACG 12830 0.0 25.829193 3 GTACATG 19960 0.0 24.964224 1 TATCAAC 13750 0.0 24.921446 2 TACATGG 19920 0.0 24.069065 2 CAACGCA 13995 0.0 23.947498 5 CATGGGT 8190 0.0 23.875525 4 TCTGTCG 1145 0.0 23.807959 8 GTCTCGC 1230 0.0 22.930784 1 GTACACG 475 0.0 22.761822 1 AACGCAG 14610 0.0 22.745382 6 ACATGGG 20780 0.0 22.100262 3 TATAACG 355 0.0 21.185564 2 ACGCAGA 15785 0.0 20.664703 7 TATACCG 275 1.4370016E-9 20.511248 5 >>END_MODULE