##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431450_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4457863
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24282643948457	33.0	31.0	34.0	30.0	34.0
2	32.35568163489995	34.0	31.0	34.0	31.0	34.0
3	32.4776775329345	34.0	31.0	34.0	31.0	34.0
4	36.03873963825268	37.0	35.0	37.0	35.0	37.0
5	35.947986288497425	37.0	35.0	37.0	35.0	37.0
6	35.99610328984987	37.0	35.0	37.0	35.0	37.0
7	35.94061078144394	37.0	35.0	37.0	35.0	37.0
8	35.92308489516165	37.0	35.0	37.0	35.0	37.0
9	37.60346717698592	39.0	37.0	39.0	35.0	39.0
10-11	37.54644759159265	39.0	37.0	39.0	35.0	39.0
12-13	37.52449559351645	39.0	37.0	39.0	35.0	39.0
14-15	38.84536256497788	40.0	38.0	41.0	35.0	41.0
16-17	38.89954929077004	40.0	38.0	41.0	35.0	41.0
18-19	38.99642485648393	40.5	38.5	41.0	35.0	41.0
20-21	39.0085251610469	41.0	39.0	41.0	35.0	41.0
22-23	38.914047380998475	40.0	38.0	41.0	35.0	41.0
24-25	38.84610608266786	40.5	38.0	41.0	35.0	41.0
26-27	38.6856936832738	40.0	38.0	41.0	34.5	41.0
28-29	38.57057619312213	40.0	38.0	41.0	34.0	41.0
30-31	38.34429635904019	40.0	38.0	41.0	34.0	41.0
32-33	38.0768137109642	40.0	37.0	41.0	33.5	41.0
34-35	37.87616914203061	40.0	37.0	41.0	33.0	41.0
36-37	37.694326182747204	40.0	37.0	41.0	33.0	41.0
38-39	37.56730276367847	40.0	37.0	41.0	33.0	41.0
40-41	37.42151329011233	40.0	37.0	41.0	33.0	41.0
42-43	37.26396941763352	40.0	36.5	41.0	32.5	41.0
44-45	37.00492119205996	40.0	36.0	41.0	31.5	41.0
46-47	36.61492793744446	40.0	36.0	41.0	30.5	41.0
48-49	36.296029622265195	40.0	36.0	41.0	30.0	41.0
50-51	36.14308077659632	40.0	36.0	41.0	29.0	41.0
52-53	36.00528549217417	40.0	35.0	41.0	28.5	41.0
54-55	35.84971106110708	40.0	35.0	41.0	28.0	41.0
56-57	35.636258898041504	39.0	35.0	41.0	28.0	41.0
58-59	35.47821478587386	39.0	35.0	41.0	27.5	41.0
60-61	35.270623727108706	39.0	35.0	41.0	27.0	41.0
62-63	35.040188763091194	38.5	35.0	41.0	26.5	41.0
64-65	34.798144985613064	38.0	35.0	41.0	26.0	41.0
66-67	34.54547795659041	37.0	35.0	40.0	26.0	41.0
68-69	34.25267701138415	37.0	34.0	40.0	26.0	41.0
70-71	33.92294177725964	36.5	34.0	39.5	25.5	41.0
72-73	33.60398614762275	36.0	34.0	39.0	25.0	41.0
74-75	33.30213860318274	35.5	34.0	39.0	25.0	41.0
76-77	32.95286687814318	35.0	34.0	38.5	24.0	40.0
78-79	32.638529829202916	35.0	34.0	37.0	23.5	39.0
80-81	32.33370271809609	35.0	33.0	37.0	23.0	39.0
82-83	32.07626927969747	35.0	33.0	36.0	23.0	39.0
84-85	31.840458421445433	35.0	33.0	36.0	22.5	37.5
86-87	31.589733242138667	35.0	33.0	36.0	21.5	37.0
88-89	31.42787149358336	35.0	33.0	35.0	20.5	37.0
90-91	31.23545508240159	35.0	33.0	35.0	20.0	36.0
92-93	31.035377040523677	35.0	33.0	35.0	18.5	36.0
94-95	30.867677068586453	35.0	33.0	35.0	16.5	36.0
96-97	30.69701267176672	35.0	33.0	35.0	5.0	36.0
98-99	30.5306957616239	35.0	33.0	35.0	2.0	35.5
100	30.41912212196741	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11169818433319989
1101	2	0.22765084304111838
1101	3	0.20850575923192594
1101	4	0.17811905279746298
1101	5	0.17270961114403605
1101	6	0.18154534457389104
1101	7	0.1854462909982999
1101	8	0.21993106211005653
1101	9	0.32618775505217457
1101	10-11	0.28139916170547963
1101	12-13	0.26390990580974005
1101	14-15	0.39287320663657255
1101	16-17	0.41816808184046295
1101	18-19	0.3957482015413518
1101	20-21	0.37477924162792675
1101	22-23	0.29482505877120246
1101	24-25	0.3046116337097047
1101	26-27	0.31181731242607214
1101	28-29	0.33434181845969846
1101	30-31	0.33456704288546035
1101	32-33	0.351659092957199
1101	34-35	0.4435796708994104
1101	36-37	0.45819439900150627
1101	38-39	0.4074841547616046
1101	40-41	0.43678005934400943
1101	42-43	0.42209358141480635
1101	44-45	0.37295416902805556
1101	46-47	0.42867548194443117
1101	48-49	0.42090523363714283
1101	50-51	0.426644078750833
1101	52-53	0.46026609071562063
1101	54-55	0.3720319925948701
1101	56-57	0.3976000795242953
1101	58-59	0.45029116477754627
1101	60-61	0.4523308507514088
1101	62-63	0.47238688295026776
1101	64-65	0.4338248603496453
1101	66-67	0.45291692126480854
1101	68-69	0.4086040573966514
1101	70-71	0.4667866732349921
1101	72-73	0.4518846780797503
1101	74-75	0.41361327816596827
1101	76-77	0.45078909866025185
1101	78-79	0.409552631618773
1101	80-81	0.3590096185739071
1101	82-83	0.3473136113713906
1101	84-85	0.36878349360311447
1101	86-87	0.39066930386434606
1101	88-89	0.3897546889184298
1101	90-91	0.3882485754653189
1101	92-93	0.39462675579058626
1101	94-95	0.37327743574825334
1101	96-97	0.46225624617963135
1101	98-99	0.508057077659636
1101	100	0.5731209321824373
1102	1	0.1344731275769675
1102	2	0.25187769176825014
1102	3	0.22886177775263405
1102	4	0.17617384399902392
1102	5	0.13740967234479484
1102	6	0.11153167273268849
1102	7	0.17407758079256297
1102	8	0.18087451354984552
1102	9	0.20053794927413549
1102	10-11	0.257272727853433
1102	12-13	0.26087889480147197
1102	14-15	0.4082747953391319
1102	16-17	0.42544644933766307
1102	18-19	0.37128665811792416
1102	20-21	0.33002795428799203
1102	22-23	0.33107463201780973
1102	24-25	0.31837618069032914
1102	26-27	0.3912524010727836
1102	28-29	0.43373838523854147
1102	30-31	0.3637386457318499
1102	32-33	0.3900220984338105
1102	34-35	0.430243017936057
1102	36-37	0.4055471513838498
1102	38-39	0.40371696466323215
1102	40-41	0.48617973335948506
1102	42-43	0.41292841330150765
1102	44-45	0.42863431559522525
1102	46-47	0.44982274450828186
1102	48-49	0.4035322016192282
1102	50-51	0.29467749688419786
1102	52-53	0.22933178005803967
1102	54-55	0.29428669002869157
1102	56-57	0.35847426711958263
1102	58-59	0.3138392278284954
1102	60-61	0.3063063043686185
1102	62-63	0.33354685765184655
1102	64-65	0.3059879139687496
1102	66-67	0.3475087169014657
1102	68-69	0.281602525017675
1102	70-71	0.2731848274577686
1102	72-73	0.2807694318528817
1102	74-75	0.3306930499768015
1102	76-77	0.35142675081651475
1102	78-79	0.33659636735104925
1102	80-81	0.335018683838463
1102	82-83	0.322111051008406
1102	84-85	0.3254393518061356
1102	86-87	0.3293804378405234
1102	88-89	0.3186718130175237
1102	90-91	0.2892963828575503
1102	92-93	0.25227315383467896
1102	94-95	0.31573931900111063
1102	96-97	0.37748736952040574
1102	98-99	0.33785504434691305
1102	100	0.261644058676076
1103	1	0.0857736568657046
1103	2	0.16930926141909453
1103	3	0.1518242000919301
1103	4	0.12092378624542732
1103	5	0.10643718350197418
1103	6	0.09087356790939793
1103	7	0.11650828158393978
1103	8	0.16094522848830195
1103	9	0.23011412984971003
1103	10-11	0.21870492512746864
1103	12-13	0.23352990768717063
1103	14-15	0.3248899948599302
1103	16-17	0.2648170270985375
1103	18-19	0.22578704348264012
1103	20-21	0.2482414463466185
1103	22-23	0.20064032494856576
1103	24-25	0.23296407553584686
1103	26-27	0.219408814175587
1103	28-29	0.20346507271694492
1103	30-31	0.21394798975317286
1103	32-33	0.2365194703894744
1103	34-35	0.22631107109798165
1103	36-37	0.2456503821099929
1103	38-39	0.2562544431717342
1103	40-41	0.2603865668880516
1103	42-43	0.26703183211017034
1103	44-45	0.2722335301462664
1103	46-47	0.29987824929519036
1103	48-49	0.29395185051823347
1103	50-51	0.2934474474307578
1103	52-53	0.2718313251845501
1103	54-55	0.28001224901197475
1103	56-57	0.327823971809579
1103	58-59	0.30799895895226825
1103	60-61	0.325979633654093
1103	62-63	0.2684922319462899
1103	64-65	0.2812891526556882
1103	66-67	0.2786248742120492
1103	68-69	0.24127962617540533
1103	70-71	0.2869248687950332
1103	72-73	0.26390216086890916
1103	74-75	0.29953718067779533
1103	76-77	0.27483014488824153
1103	78-79	0.26984736347286997
1103	80-81	0.24473994350069006
1103	82-83	0.1839977640006154
1103	84-85	0.2114248530971672
1103	86-87	0.24161694343086637
1103	88-89	0.25054924454628846
1103	90-91	0.25064148974770717
1103	92-93	0.23298769363559657
1103	94-95	0.27712955547399076
1103	96-97	0.3354341626112358
1103	98-99	0.34045830062774485
1103	100	0.3517539847310225
1104	1	0.1256668825701297
1104	2	0.16529539919416436
1104	3	0.1797965427975612
1104	4	0.14552384151654962
1104	5	0.11736375406188415
1104	6	0.17786012085421277
1104	7	0.1193627571010154
1104	8	0.11968155595805996
1104	9	0.2068900034853911
1104	10-11	0.233564668532793
1104	12-13	0.2155461000901937
1104	14-15	0.33931108612884486
1104	16-17	0.3233103539865141
1104	18-19	0.2864863695488964
1104	20-21	0.3040153673825259
1104	22-23	0.26220984571386197
1104	24-25	0.2544076268905613
1104	26-27	0.2930886434166027
1104	28-29	0.3117401124400061
1104	30-31	0.3376853612656774
1104	32-33	0.31644741577698454
1104	34-35	0.30953202177110484
1104	36-37	0.3548915896597151
1104	38-39	0.29828760163647416
1104	40-41	0.2382552713421191
1104	42-43	0.304332877846484
1104	44-45	0.24759700924715844
1104	46-47	0.34991974913772594
1104	48-49	0.3513333673297723
1104	50-51	0.2737938934123392
1104	52-53	0.2135147219876572
1104	54-55	0.27376755916609596
1104	56-57	0.2871363705440402
1104	58-59	0.31156193225286444
1104	60-61	0.30735541376155595
1104	62-63	0.26024020717393626
1104	64-65	0.2734839909044453
1104	66-67	0.24129782530140886
1104	68-69	0.25155926223180103
1104	70-71	0.2480675116612474
1104	72-73	0.2815107518380131
1104	74-75	0.21796686256340791
1104	76-77	0.23569146400026852
1104	78-79	0.2969562661863918
1104	80-81	0.2103545327474592
1104	82-83	0.2121738125146777
1104	84-85	0.19746891884728868
1104	86-87	0.2156898085102945
1104	88-89	0.13158204217262437
1104	90-91	0.18638857599719216
1104	92-93	0.2639353746125508
1104	94-95	0.20818405382001615
1104	96-97	0.18362946644529643
1104	98-99	0.24253379691025856
1104	100	0.2750091827472154
1105	1	0.11661026386940421
1105	2	0.200667430529478
1105	3	0.1783758967268625
1105	4	0.06864668529269835
1105	5	0.08692038630269394
1105	6	0.11347075551012153
1105	7	0.1366324681753497
1105	8	0.15834411267579185
1105	9	0.2066117581846143
1105	10-11	0.17635821448948263
1105	12-13	0.11135411664568551
1105	14-15	0.23891135573968114
1105	16-17	0.21302221095099583
1105	18-19	0.22406315284606393
1105	20-21	0.2044213432267199
1105	22-23	0.21110465537918088
1105	24-25	0.20111829955796878
1105	26-27	0.23642549433765936
1105	28-29	0.18652901818666834
1105	30-31	0.21251180354398969
1105	32-33	0.21801851280356033
1105	34-35	0.2599745842139569
1105	36-37	0.27460495375670746
1105	38-39	0.24232814732903307
1105	40-41	0.22283821318254127
1105	42-43	0.16674869357876076
1105	44-45	0.24356138902810187
1105	46-47	0.30991573106291526
1105	48-49	0.2874484589437216
1105	50-51	0.24010863116915715
1105	52-53	0.20538903762827232
1105	54-55	0.16685713685565418
1105	56-57	0.17759498827242481
1105	58-59	0.16983405516455718
1105	60-61	0.14727398040056983
1105	62-63	0.1428125732826473
1105	64-65	0.18033444692191125
1105	66-67	0.21417397714834152
1105	68-69	0.18751726044791184
1105	70-71	0.19761093416209974
1105	72-73	0.21222845174114013
1105	74-75	0.15458286660926746
1105	76-77	0.2514066168069178
1105	78-79	0.1953260889983639
1105	80-81	0.20075237603090557
1105	82-83	0.16389970608617688
1105	84-85	0.16868098656928154
1105	86-87	0.15684752021526904
1105	88-89	0.18068737542259328
1105	90-91	0.23087503725431446
1105	92-93	0.18579080218235688
1105	94-95	0.25225286356389276
1105	96-97	0.23909655062878699
1105	98-99	0.2497483962745939
1105	100	0.2954654936979999
1106	1	0.11972100045952061
1106	2	0.17571454875967163
1106	3	0.1601859275262285
1106	4	0.15971654283053738
1106	5	0.1188263001950034
1106	6	0.11304402999400054
1106	7	0.13959771399993315
1106	8	0.1744002937606055
1106	9	0.17634814240243202
1106	10-11	0.18473549204261985
1106	12-13	0.20599430014017628
1106	14-15	0.2189190462934789
1106	16-17	0.2112378621155031
1106	18-19	0.23729701030850947
1106	20-21	0.21660018623664
1106	22-23	0.20894229199637238
1106	24-25	0.24314077531268907
1106	26-27	0.2755951447805032
1106	28-29	0.26823219675167564
1106	30-31	0.2732308632149696
1106	32-33	0.3128538039096256
1106	34-35	0.3443750065408082
1106	36-37	0.3413752390199676
1106	38-39	0.3108709591564036
1106	40-41	0.36098107584911787
1106	42-43	0.43673616403513194
1106	44-45	0.46335840968728803
1106	46-47	0.43011027588390505
1106	48-49	0.3926197520745376
1106	50-51	0.4306453237613397
1106	52-53	0.3776690242405536
1106	54-55	0.36437447362609277
1106	56-57	0.4016154723814864
1106	58-59	0.39729272182058395
1106	60-61	0.4479550716601963
1106	62-63	0.42303128507384
1106	64-65	0.42481008641505724
1106	66-67	0.43383214631952427
1106	68-69	0.4782033837982951
1106	70-71	0.4972825456342349
1106	72-73	0.4759270242299607
1106	74-75	0.45769391050964003
1106	76-77	0.45476881115992285
1106	78-79	0.4146457467501463
1106	80-81	0.3735701899899624
1106	82-83	0.3714881503215821
1106	84-85	0.38853870100384214
1106	86-87	0.3617884063356769
1106	88-89	0.3064646582321018
1106	90-91	0.30757253310230226
1106	92-93	0.3694477928416404
1106	94-95	0.451595004042197
1106	96-97	0.41866318445912043
1106	98-99	0.5033928092296769
1106	100	0.559716353169101
1107	1	0.04400638022080017
1107	2	0.13614006167087922
1107	3	0.07393779246161358
1107	4	0.10540944097134286
1107	5	0.1070108177890674
1107	6	0.08884550193092622
1107	7	0.03979677170218565
1107	8	0.06747018693646822
1107	9	0.07814309838120437
1107	10-11	0.07842798870294843
1107	12-13	0.027784115450664615
1107	14-15	0.16110821966480415
1107	16-17	0.1499633715050308
1107	18-19	0.10203669976255725
1107	20-21	0.11137072193785258
1107	22-23	0.12923812743304808
1107	24-25	0.1484289514310504
1107	26-27	0.17717278133408598
1107	28-29	0.2171429236859339
1107	30-31	0.2170021412433769
1107	32-33	0.16283366052668669
1107	34-35	0.14912854957682953
1107	36-37	0.12373119951253386
1107	38-39	0.12511183866531894
1107	40-41	0.14534533363448077
1107	42-43	0.1151239765344414
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2216	72-73	-0.7885542834784545
2216	74-75	-0.8263452808864926
2216	76-77	-0.6706113532071143
2216	78-79	-0.6371730274407099
2216	80-81	-0.7431404366285221
2216	82-83	-0.8334445045471455
2216	84-85	-0.819362617162195
2216	86-87	-0.847194711621615
2216	88-89	-0.7836098159937563
2216	90-91	-0.8375451771944711
2216	92-93	-0.7990963276602443
2216	94-95	-0.9035580577241298
2216	96-97	-1.0868272218153265
2216	98-99	-1.0769471262566803
2216	100	-1.0875201631630418
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	270.0
3	720.0
4	597.0
5	480.0
6	864.0
7	1931.0
8	3294.0
9	4851.0
10	7021.0
11	9637.0
12	13689.0
13	19187.0
14	19903.0
15	23745.0
16	31023.0
17	44259.0
18	49458.0
19	27885.0
20	18095.0
21	16114.0
22	16181.0
23	16667.0
24	18812.0
25	22368.0
26	27542.0
27	34735.0
28	43675.0
29	54831.0
30	68178.0
31	85534.0
32	107511.0
33	135945.0
34	184275.0
35	283000.0
36	416984.0
37	791530.0
38	1218207.0
39	634646.0
40	4219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.077527525528076	20.98830212748576	14.411104742214548	23.523065604771613
2	14.241352863468437	22.132017067370622	39.71752833140004	23.909101737760896
3	16.063353339218075	27.964076893483526	31.973920192558978	23.99864957473942
4	11.151367648514634	18.715720679192135	38.26885964528304	31.864052027010192
5	10.956634959348499	37.8915463453132	35.06803815287262	16.083780542465686
6	27.152113019175335	39.270094213303544	18.74256790753776	14.83522485998336
7	23.841109518170477	34.30800811958555	23.62928156383451	18.22160079840946
8	24.6984261292911	34.082025400960056	21.908995408786677	19.310553060962167
9	24.824674961971688	15.852191958344166	23.397376725125916	35.925756354558224
10-11	22.502425221949313	27.18469646810508	29.540919619035993	20.771958690909614
12-13	24.40021149147024	25.367491105042934	29.976818489038358	20.255478914448467
14-15	21.663350802839837	26.692262637950066	29.236553478651096	22.407833080559
16-17	18.967126176825083	29.82440240985423	29.700755720846512	21.507715692474175
18-19	18.727560716872635	26.890104070044323	34.418453415908026	19.963881797175016
20-21	20.46337000486556	26.212514830536517	34.18779356835327	19.136321596244656
22-23	19.930569871707586	26.54641921476725	34.23440783173462	19.288603081790534
24-25	19.599902508805556	26.334377075347167	34.4690818207075	19.596638595139776
26-27	19.41719608700402	26.082699266442237	34.66083412612725	19.839270520426492
28-29	19.444837322236197	26.190157063505886	34.6276728012057	19.737332813052223
30-31	19.94281590449621	26.831782162492473	33.957708759748286	19.26769317326303
32-33	19.210519320448885	26.466476193873085	34.569140827723295	19.75386365795474
34-35	19.40093968266502	26.04615098339836	34.37845708678184	20.17445224715478
36-37	20.10136444370415	26.64141809271757	33.509804152498695	19.747413311079594
38-39	19.984530496300533	26.59773211153566	33.68696418392648	19.73077320823733
40-41	19.635980570286563	26.46762879049507	33.80800197585744	20.088388663360934
42-43	19.588275138727234	26.542857806227055	33.59985741713016	20.26900963791556
44-45	20.163116291720527	26.71133306601538	33.11485044682289	20.0107001954412
46-47	20.031277455119277	27.377206041441337	32.31578228351892	20.27573421992046
48-49	20.072380353756568	27.0743372434113	32.205623374282546	20.64765902854959
50-51	20.15367062670867	26.954352400891835	31.947509310125373	20.94446766227412
52-53	20.200826636308065	27.263912333929664	31.626263929158277	20.908997100603994
54-55	20.407234144883894	27.814412656994342	31.320264417188376	20.45808878093339
56-57	20.02638214628631	27.32492664148099	31.44456832886153	21.20412288337117
58-59	19.85268450052048	26.775694120391975	31.957805377077424	21.413816002010122
60-61	20.381271372669364	27.343848843688672	31.318279257283194	20.956600526358766
62-63	20.150410196370675	27.149411709019567	31.635661813612852	21.06451628099691
64-65	19.99974202564826	26.890988451161252	31.971210062345673	21.138059460844822
66-67	20.015193393868376	27.03858814720117	31.446040739067872	21.500177719862577
68-69	20.653429034787507	27.18900925037039	31.243646700524014	20.913915014318093
70-71	20.712718334048176	26.926063876561066	31.224056988932773	21.13716080045798
72-73	20.324940447923144	26.99514318856367	31.625994338542927	21.05392202497026
74-75	20.285853013688996	26.99425429363322	31.33355414229347	21.386338550384316
76-77	20.39812573872279	27.039581521459947	30.906322154808258	21.655970585009005
78-79	20.56371335579417	27.245939899498257	30.99202011380866	21.198326630898915
80-81	20.13286411000069	26.973944690538943	31.224613677001738	21.668577522458634
82-83	19.985856291622852	27.012452856642135	31.36286014721346	21.638830704521556
84-85	20.190334503108684	26.89154558400085	31.131887758993376	21.786232153897092
86-87	20.34615244413662	27.022468728005855	30.864377266010333	21.767001561847184
88-89	19.894711883249887	26.958892635327736	31.008927371702537	22.137468109719837
90-91	19.79468637775544	26.758426627287555	30.9180318013362	22.5288551936208
92-93	19.964527794916684	26.981846544409855	30.771504197140487	22.282121463532977
94-95	19.777480824332198	26.75636285816769	31.001322831141287	22.464833486358824
96-97	19.693982284944088	26.79726634822442	30.93794279367852	22.570808573152977
98-99	19.62067952192093	26.69275927354952	30.919761588572044	22.766799615957503
100	19.876770247195285	26.87691291680388	30.52126898784437	22.725047848156464
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4637.0
1	2953.0
2	9045.5
3	15353.5
4	8839.0
5	3981.5
6	6934.5
7	16006.0
8	24513.5
9	25352.5
10	17284.0
11	8766.0
12	6880.5
13	7039.0
14	7538.0
15	8104.0
16	8641.5
17	9310.0
18	10082.5
19	11000.5
20	12233.0
21	13838.5
22	16578.0
23	20964.5
24	26783.5
25	34059.0
26	43498.5
27	54791.0
28	68485.5
29	82278.0
30	92582.5
31	101955.5
32	110847.5
33	118517.0
34	126871.0
35	133469.5
36	138003.5
37	141953.5
38	143582.5
39	143971.0
40	144008.0
41	141835.5
42	138103.5
43	135696.0
44	133301.0
45	130559.0
46	128682.5
47	127622.5
48	128224.0
49	129810.5
50	129906.0
51	125378.5
52	116875.5
53	108783.5
54	103715.0
55	99911.0
56	92253.5
57	82827.5
58	76245.0
59	70346.0
60	60798.0
61	49568.0
62	40344.5
63	32147.0
64	24830.5
65	18651.5
66	14113.0
67	10596.5
68	7737.5
69	5592.0
70	3893.5
71	2741.0
72	2121.5
73	1732.5
74	1295.5
75	885.0
76	580.0
77	397.5
78	259.5
79	138.5
80	73.5
81	32.5
82	18.0
83	8.0
84	2.5
85	1.5
86	2.0
87	2.5
88	1.5
89	0.5
90	0.5
91	1.0
92	0.5
93	0.0
94	1.0
95	2.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04892478750468555
2	0.0
3	1.7945818433630643E-4
4	0.06954004643031875
5	0.004867803250122312
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.012090995169658647
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.495906895299384E-4
26-27	0.0
28-29	3.7013250519363206E-4
30-31	0.003477002321515937
32-33	1.906743208573256E-4
34-35	0.0018282302529261216
36-37	0.004161186649298106
38-39	0.0013459363825222984
40-41	0.0011552620616649726
42-43	0.0023441725328930026
44-45	1.1216136521019152E-5
46-47	4.37429324319747E-4
48-49	5.271584164879001E-4
50-51	0.001839446389447141
52-53	8.524263755974556E-4
54-55	0.0013235041094802598
56-57	0.006796978731737606
58-59	0.009488851496782202
60-61	0.0015029622938165664
62-63	0.002691872765044597
64-65	0.001244991153833126
66-67	1.0094522868917237E-4
68-69	9.309393312445896E-4
70-71	3.364840956305746E-4
72-73	0.0
74-75	2.804034130254788E-4
76-77	0.0
78-79	6.729681912611492E-4
80-81	0.0
82-83	0.0011328297886229344
84-85	6.056713721350343E-4
86-87	1.1216136521019152E-5
88-89	0.0
90-91	0.0
92-93	1.4580977477324897E-4
94-95	0.0
96-97	3.7013250519363206E-4
98-99	5.15942279966881E-4
100	2.4675500346242135E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4457863.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.820852765751454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14523231155783	43.024649403148295
2	16.878258984511206	19.85587173340311
3	5.1155416307607355	9.02701563240148
4	1.9818620115090342	4.662992543240357
5	0.936267252061001	2.7536019091437423
6	0.5301540147452266	1.87104667467006
7	0.3271385966530796	1.3469799859407743
8	0.2159348014717785	1.0161175331498602
9	0.14956598860229872	0.7917839094905886
>10	0.6597948236999434	6.691038139887535
>50	0.03580911829593022	1.4562923583150682
>100	0.021764549548354273	2.4760067036874114
>500	0.0015673227751649714	0.6421233410744843
>1k	8.027748266562269E-4	0.7147069132258529
>5k	1.1468211809364312E-4	0.5207680387069543
>10k+	1.911368634894052E-4	3.1490051805144823
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40523	0.9090230004825182	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35449	0.7952016470672159	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27250	0.6112794403955438	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24072	0.5399896766679461	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12751	0.2860339135590304	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8834	0.19816670005336637	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7900	0.1772149570321026	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6426	0.14414978656813812	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.2432273042038304E-5	0.0	0.0	0.0	0.0
9	2.2432273042038304E-5	0.0	0.0	4.486454608407661E-5	0.0
10-11	2.2432273042038304E-5	0.0	0.0	5.608068260509576E-5	0.0
12-13	2.2432273042038304E-5	0.0	0.0	6.72968191261149E-5	0.0
14-15	2.2432273042038304E-5	0.0	0.0	6.72968191261149E-5	1.1216136521019152E-5
16-17	2.2432273042038304E-5	0.0	0.0	8.972909216815322E-5	2.2432273042038304E-5
18-19	3.364840956305746E-5	0.0	0.0	1.0094522868917237E-4	2.2432273042038304E-5
20-21	4.486454608407661E-5	0.0	0.0	2.4675500346242135E-4	2.2432273042038304E-5
22-23	4.486454608407661E-5	0.0	0.0	8.075618295133789E-4	2.2432273042038304E-5
24-25	4.486454608407661E-5	0.0	0.0	0.001929175481615294	2.2432273042038304E-5
26-27	4.486454608407661E-5	0.0	0.0	0.0035218668676000138	2.2432273042038304E-5
28-29	4.486454608407661E-5	0.0	0.0	0.007357785557788564	2.2432273042038304E-5
30-31	4.486454608407661E-5	0.0	0.0	0.017373795471058665	2.2432273042038304E-5
32-33	4.486454608407661E-5	0.0	0.0	0.03539812686033644	2.2432273042038304E-5
34-35	4.486454608407661E-5	0.0	0.0	0.06410022021762446	2.2432273042038304E-5
36-37	4.486454608407661E-5	0.0	0.0	0.10797774628785137	2.2432273042038304E-5
38-39	8.972909216815322E-5	0.0	0.0	0.16802894122138792	2.2432273042038304E-5
40-41	8.972909216815322E-5	0.0	0.0	0.2367165612761092	2.2432273042038304E-5
42-43	8.972909216815322E-5	0.0	0.0	0.30614444634121774	2.2432273042038304E-5
44-45	8.972909216815322E-5	0.0	0.0	0.3914207323105264	2.2432273042038304E-5
46-47	8.972909216815322E-5	0.0	0.0	0.49270244509532934	2.2432273042038304E-5
48-49	1.1216136521019151E-4	0.0	0.0	0.6036300352882087	2.2432273042038304E-5
50-51	1.1216136521019151E-4	0.0	0.0	0.7204460971546232	2.2432273042038304E-5
52-53	1.2337750173121065E-4	0.0	0.0	0.8384622856287867	2.2432273042038304E-5
54-55	1.345936382522298E-4	0.0	0.0	0.9717436359080573	2.2432273042038304E-5
56-57	1.345936382522298E-4	0.0	0.0	1.1275582044580554	2.2432273042038304E-5
58-59	1.345936382522298E-4	0.0	0.0	1.299411848233111	2.2432273042038304E-5
60-61	1.345936382522298E-4	0.0	0.0	1.4791728682554848	2.2432273042038304E-5
62-63	1.5702591129426814E-4	0.0	0.0	1.6588329430491695	2.2432273042038304E-5
64-65	1.5702591129426814E-4	0.0	0.0	1.8630002761412812	2.2432273042038304E-5
66-67	1.682420478152873E-4	0.0	0.0	2.1033284333771585	2.2432273042038304E-5
68-69	2.0189045737834473E-4	0.0	0.0	2.3659879184263852	2.2432273042038304E-5
70-71	2.0189045737834473E-4	0.0	0.0	2.654343572245266	2.2432273042038304E-5
72-73	2.0189045737834473E-4	0.0	0.0	2.9729042817152527	2.2432273042038304E-5
74-75	2.0189045737834473E-4	0.0	0.0	3.296456171937092	2.2432273042038304E-5
76-77	2.0189045737834473E-4	0.0	0.0	3.630214746393059	2.2432273042038304E-5
78-79	2.0189045737834473E-4	0.0	0.0	4.013672021773662	2.2432273042038304E-5
80-81	2.0189045737834473E-4	0.0	0.0	4.426190306880224	2.2432273042038304E-5
82-83	2.0189045737834473E-4	0.0	0.0	4.8626550434591636	2.2432273042038304E-5
84-85	2.0189045737834473E-4	0.0	0.0	5.3016209784822905	2.2432273042038304E-5
86-87	2.0189045737834473E-4	0.0	0.0	5.773988568065013	2.2432273042038304E-5
88	2.0189045737834473E-4	0.0	0.0	6.152073314052047	2.2432273042038304E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15260	0.0	58.304314	1
GTATCAA	27465	0.0	40.6006	1
TATAACG	530	0.0	39.930107	2
TACCTGG	4210	0.0	38.762447	2
ATAACGC	550	0.0	35.91289	3
GTACCTG	5140	0.0	32.495926	1
TAACGCA	700	0.0	31.576473	4
TATCAAC	36205	0.0	30.616451	2
ATCAACG	36200	0.0	30.607689	3
TCAACGC	36585	0.0	30.272736	4
GTGGTAT	5760	0.0	30.141691	1
ACCTGGG	5505	0.0	29.302225	3
CAACGCA	38125	0.0	29.037857	5
TGGTATC	5965	0.0	28.777048	2
AACGCAG	39430	0.0	28.134834	6
TATCACG	435	0.0	25.946918	2
ACGCAGA	44200	0.0	24.99221	7
CGCAGAG	44200	0.0	24.907131	8
GCAGAGT	46175	0.0	23.516039	9
TCTGTCG	1205	0.0	23.40579	8
>>END_MODULE