FastQCFastQC Report
Wed 25 May 2016
SRR2431449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369652
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA469421.393081540764447No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65900.19556915669629982No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36380.10796367102596945No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54750.042.586361
TACCTGG30600.037.4813842
GTACCTG35500.032.4422341
GTATCAA84250.031.6363351
TCTGTCG9550.031.4976928
GTCTCGC8800.029.9142671
ATCAACG94850.027.6031513
TCAACGC95350.027.4091074
GTACATG151700.026.3394281
TATCAAC101300.025.9851862
TACATGG153300.024.8058222
CATGGGT67300.024.445214
CAACGCA107450.024.4100445
ACCTGGG46450.024.185383
CTGTCGC13050.024.1305059
ACATGGG157950.023.3907263
AACGCAG112750.023.2198876
TCTCGCT10800.022.6322042
TCGCTCT11150.022.3430164
CGCTCTG9800.022.0634065
Produced by FastQC (version 0.11.5)