##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431449_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3369652
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22124836630014	33.0	31.0	34.0	30.0	34.0
2	32.35981341693445	34.0	31.0	34.0	31.0	34.0
3	32.45151457776649	34.0	31.0	34.0	30.0	34.0
4	36.01105366370177	37.0	35.0	37.0	35.0	37.0
5	35.94184800092116	37.0	35.0	37.0	35.0	37.0
6	35.990009947614766	37.0	35.0	37.0	35.0	37.0
7	35.92815697288622	37.0	35.0	37.0	35.0	37.0
8	35.91179296853206	37.0	35.0	37.0	35.0	37.0
9	37.58680362245122	39.0	37.0	39.0	35.0	39.0
10-11	37.52160816606582	39.0	37.0	39.0	34.5	39.0
12-13	37.508936085981574	39.0	37.0	39.0	35.0	39.0
14-15	38.80412294800769	40.0	38.0	41.0	34.0	41.0
16-17	38.85965998862791	40.0	38.0	41.0	35.0	41.0
18-19	38.96721976037882	40.5	38.5	41.0	35.0	41.0
20-21	38.97203034616037	40.0	38.5	41.0	35.0	41.0
22-23	38.86555985603261	40.0	38.0	41.0	35.0	41.0
24-25	38.782983969858016	40.0	38.0	41.0	35.0	41.0
26-27	38.60876286334613	40.0	38.0	41.0	34.0	41.0
28-29	38.48989776985873	40.0	38.0	41.0	34.0	41.0
30-31	38.23511641558238	40.0	37.5	41.0	34.0	41.0
32-33	37.933111045294886	40.0	37.0	41.0	33.5	41.0
34-35	37.71457720263101	40.0	36.5	41.0	33.0	41.0
36-37	37.5165341999708	40.0	36.0	41.0	33.0	41.0
38-39	37.374439259603065	40.0	36.0	41.0	33.0	41.0
40-41	37.21564407837961	40.0	36.0	41.0	32.0	41.0
42-43	37.04796504208743	40.0	36.0	41.0	31.5	41.0
44-45	36.765981472270724	40.0	36.0	41.0	31.0	41.0
46-47	36.357102899646605	40.0	35.0	41.0	30.0	41.0
48-49	36.019190260596645	40.0	35.0	41.0	28.5	41.0
50-51	35.86544367192814	40.0	35.0	41.0	28.0	41.0
52-53	35.7225050242577	40.0	35.0	41.0	27.0	41.0
54-55	35.5622712671813	39.5	35.0	41.0	27.0	41.0
56-57	35.346274778523124	39.0	35.0	41.0	26.0	41.0
58-59	35.18184340697496	39.0	35.0	41.0	26.0	41.0
60-61	34.96831571924935	39.0	35.0	41.0	26.0	41.0
62-63	34.72917663307665	38.0	35.0	41.0	26.0	41.0
64-65	34.482424446203936	38.0	35.0	40.5	25.5	41.0
66-67	34.22338508546283	37.0	34.0	40.0	25.0	41.0
68-69	33.928301201429704	37.0	34.0	40.0	24.0	41.0
70-71	33.60032059690437	36.0	34.0	39.0	23.5	41.0
72-73	33.28221816377477	36.0	34.0	39.0	23.0	41.0
74-75	32.98323343182025	35.0	34.0	39.0	22.5	41.0
76-77	32.63653205138097	35.0	33.5	37.5	22.0	40.0
78-79	32.328412251472855	35.0	33.0	37.0	20.5	39.0
80-81	32.03176203358685	35.0	33.0	37.0	20.0	39.0
82-83	31.77647142791006	35.0	33.0	36.0	20.0	39.0
84-85	31.541114483038605	35.0	33.0	36.0	19.5	37.0
86-87	31.294495841113562	35.0	33.0	36.0	18.5	37.0
88-89	31.138821753700384	35.0	33.0	35.0	18.0	37.0
90-91	30.945772738549856	35.0	33.0	35.0	13.0	36.0
92-93	30.744646628197806	35.0	33.0	35.0	7.0	36.0
94-95	30.574007939098756	35.0	33.0	35.0	2.0	36.0
96-97	30.40133625074637	35.0	33.0	35.0	2.0	36.0
98-99	30.23340110492122	35.0	33.0	35.0	2.0	35.0
100	30.117484238728508	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18834044543750394
1101	2	0.26578705263487024
1101	3	0.23620950642227
1101	4	0.210478850835095
1101	5	0.20636463070031397
1101	6	0.22544574510279602
1101	7	0.2010592204673216
1101	8	0.2751620797260159
1101	9	0.39301214674097196
1101	10-11	0.3172267604818373
1101	12-13	0.39387697502566965
1101	14-15	0.4795090063410683
1101	16-17	0.4769635089969242
1101	18-19	0.4655084537637606
1101	20-21	0.418903092044026
1101	22-23	0.39639124258258107
1101	24-25	0.4249088958328784
1101	26-27	0.3789337871273446
1101	28-29	0.3608654002888514
1101	30-31	0.44006511157193984
1101	32-33	0.4880391204214476
1101	34-35	0.5394543584046687
1101	36-37	0.5499195652952906
1101	38-39	0.4399683471915736
1101	40-41	0.5064757635210881
1101	42-43	0.4540919327031858
1101	44-45	0.4886753177783376
1101	46-47	0.5233124990256712
1101	48-49	0.5236889998766046
1101	50-51	0.5169966625039777
1101	52-53	0.47481597516723184
1101	54-55	0.49310601263178455
1101	56-57	0.4996036107524304
1101	58-59	0.4769351044957517
1101	60-61	0.5178442994510206
1101	62-63	0.5140833047486808
1101	64-65	0.46754047340046867
1101	66-67	0.4333444119452281
1101	68-69	0.4092518425521021
1101	70-71	0.4404834290492943
1101	72-73	0.4503535368091818
1101	74-75	0.3846354139361807
1101	76-77	0.4523852988575072
1101	78-79	0.3941651045861079
1101	80-81	0.43931089890245545
1101	82-83	0.3555395721731003
1101	84-85	0.4041224355180546
1101	86-87	0.4027318043195294
1101	88-89	0.40123334768232155
1101	90-91	0.42652785657326575
1101	92-93	0.4627077756138007
1101	94-95	0.47682037695246393
1101	96-97	0.5369879745989223
1101	98-99	0.5640291836911544
1101	100	0.6077937893359113
1102	1	0.16351027520956762
1102	2	0.2638799918180723
1102	3	0.18294029916071253
1102	4	0.18496317344090585
1102	5	0.15879479545028374
1102	6	0.14394393914773218
1102	7	0.19191794140512286
1102	8	0.20815068977379525
1102	9	0.27925084187482696
1102	10-11	0.30775726812910165
1102	12-13	0.3261776263462366
1102	14-15	0.40706728205849885
1102	16-17	0.3762970901915281
1102	18-19	0.39081881715441824
1102	20-21	0.3152559555264034
1102	22-23	0.295013448415979
1102	24-25	0.2904196007851496
1102	26-27	0.2854632069578358
1102	28-29	0.32162296813072544
1102	30-31	0.313836858397039
1102	32-33	0.31740079819537215
1102	34-35	0.325289543511893
1102	36-37	0.2545745638843684
1102	38-39	0.2080010997078432
1102	40-41	0.31642281149508733
1102	42-43	0.23828533279674957
1102	44-45	0.2521410235683703
1102	46-47	0.1677809152276808
1102	48-49	0.17601132975898537
1102	50-51	0.11110929803513869
1102	52-53	0.029058959349249847
1102	54-55	0.10269712776909756
1102	56-57	0.1265550321421074
1102	58-59	0.14172962395514332
1102	60-61	0.11490180918244874
1102	62-63	0.08999517130125412
1102	64-65	0.10609830293380185
1102	66-67	0.08943018377861733
1102	68-69	0.019378158807867862
1102	70-71	0.11969828368272317
1102	72-73	0.07195594806310623
1102	74-75	0.07873699208204954
1102	76-77	0.022445078058083823
1102	78-79	0.00994171936591215
1102	80-81	0.03507580198379401
1102	82-83	0.05918947303705124
1102	84-85	0.05213373772129515
1102	86-87	0.03360546963479649
1102	88-89	-0.021816930113345734
1102	90-91	0.023864590644329553
1102	92-93	-0.008316041892676651
1102	94-95	0.030644905039654446
1102	96-97	0.03858123561289872
1102	98-99	0.08034432187719176
1102	100	0.0979395287173972
1103	1	0.16157245463615766
1103	2	0.20206497216095443
1103	3	0.1662423010566556
1103	4	0.12340952037517638
1103	5	0.1317684305298883
1103	6	0.17581949024805255
1103	7	0.1340394767045936
1103	8	0.1344218062163378
1103	9	0.21204048083305338
1103	10-11	0.22835980005795165
1103	12-13	0.1841298083434566
1103	14-15	0.3220824202989192
1103	16-17	0.3515963748018649
1103	18-19	0.2861228341718842
1103	20-21	0.2494968908314661
1103	22-23	0.29391042015544144
1103	24-25	0.27579548616655103
1103	26-27	0.3293587245456209
1103	28-29	0.32603450182955385
1103	30-31	0.28610588787948643
1103	32-33	0.35681803345990204
1103	34-35	0.3608209577402022
1103	36-37	0.3273983890630632
1103	38-39	0.28057675904604196
1103	40-41	0.259308748896558
1103	42-43	0.33182990904271037
1103	44-45	0.3831258260694028
1103	46-47	0.3256971531113422
1103	48-49	0.2861898039110855
1103	50-51	0.19540850699979728
1103	52-53	0.24986187784906377
1103	54-55	0.2094361648972196
1103	56-57	0.2915476173755138
1103	58-59	0.2414538896499252
1103	60-61	0.23202404383204822
1103	62-63	0.26782898353079077
1103	64-65	0.28680640279868186
1103	66-67	0.2071973102838527
1103	68-69	0.19697460581127757
1103	70-71	0.22756786787810768
1103	72-73	0.2492822969439672
1103	74-75	0.2684324445516495
1103	76-77	0.2480793472405196
1103	78-79	0.24907336321280127
1103	80-81	0.157431575710838
1103	82-83	0.15797321626680372
1103	84-85	0.17607016687762567
1103	86-87	0.21478643777703965
1103	88-89	0.17688387598492028
1103	90-91	0.2069191550170686
1103	92-93	0.24314253762707239
1103	94-95	0.204345971842411
1103	96-97	0.19545527628246617
1103	98-99	0.22931839593348613
1103	100	0.2578635221870407
1104	1	0.12991452805285775
1104	2	0.16526518730146478
1104	3	0.14952857240262318
1104	4	0.18076598131372634
1104	5	0.10160573051643951
1104	6	0.13345563385433934
1104	7	0.13541906524835667
1104	8	0.12592913444008502
1104	9	0.21500404140855522
1104	10-11	0.24660102635888137
1104	12-13	0.2912670948837288
1104	14-15	0.3481224902195805
1104	16-17	0.3291343605488066
1104	18-19	0.326305703608476
1104	20-21	0.30633970379368236
1104	22-23	0.31007554049966757
1104	24-25	0.3183005285223359
1104	26-27	0.3545812605144292
1104	28-29	0.39283294206083497
1104	30-31	0.406399016829333
1104	32-33	0.33907700885319514
1104	34-35	0.3095287700301057
1104	36-37	0.3998402338929665
1104	38-39	0.42877899112887263
1104	40-41	0.42977533488552666
1104	42-43	0.41257079918658235
1104	44-45	0.5449693113457243
1104	46-47	0.5179520946200071
1104	48-49	0.4594169116158042
1104	50-51	0.42667380214569306
1104	52-53	0.3681595785348222
1104	54-55	0.37812246313575315
1104	56-57	0.3452520680948865
1104	58-59	0.4342749196351363
1104	60-61	0.420784296622152
1104	62-63	0.38174050699456075
1104	64-65	0.3516954709713289
1104	66-67	0.37047685848877876
1104	68-69	0.40081620860810574
1104	70-71	0.31723305545374103
1104	72-73	0.3713785723238203
1104	74-75	0.3814686150505082
1104	76-77	0.34719727113456145
1104	78-79	0.29676194731929684
1104	80-81	0.3898545957457955
1104	82-83	0.309846283363747
1104	84-85	0.2715912103091185
1104	86-87	0.28204205159981655
1104	88-89	0.2718159722602991
1104	90-91	0.2886093526774154
1104	92-93	0.34713877266808524
1104	94-95	0.2901265285184884
1104	96-97	0.37521593246103535
1104	98-99	0.4358990063946955
1104	100	0.4852159492409598
1105	1	0.12323265205365175
1105	2	0.19814344651361182
1105	3	0.13972652711693456
1105	4	0.12991577573489366
1105	5	0.10562079981420425
1105	6	0.13210301462056862
1105	7	0.14877908298609555
1105	8	0.1361204375025622
1105	9	0.14690365203407652
1105	10-11	0.16991022314275028
1105	12-13	0.13149175345171926
1105	14-15	0.2508908221331154
1105	16-17	0.1956713978019522
1105	18-19	0.24036410588949053
1105	20-21	0.20436879212839187
1105	22-23	0.21274946563552533
1105	24-25	0.2003368091987099
1105	26-27	0.22931680873112725
1105	28-29	0.23036193120424286
1105	30-31	0.2190138550697185
1105	32-33	0.230747390331274
1105	34-35	0.3287634021859773
1105	36-37	0.27377587235429957
1105	38-39	0.3298198773934047
1105	40-41	0.31979340326140004
1105	42-43	0.2941189225261098
1105	44-45	0.30889174374977557
1105	46-47	0.1795032131477754
1105	48-49	0.16303406820969712
1105	50-51	0.2144038773365864
1105	52-53	0.2030376315846567
1105	54-55	0.2680917332822901
1105	56-57	0.2779894756622099
1105	58-59	0.22095996586606503
1105	60-61	0.24190875641856024
1105	62-63	0.2457051054253867
1105	64-65	0.2867223109699282
1105	66-67	0.23507093268386825
1105	68-69	0.221253253251966
1105	70-71	0.2409840036058597
1105	72-73	0.24482988495535096
1105	74-75	0.18330025046218879
1105	76-77	0.25662198104863165
1105	78-79	0.2141255009351113
1105	80-81	0.13867204096691665
1105	82-83	0.1408061828499001
1105	84-85	0.14287005844528977
1105	86-87	0.1044626499372967
1105	88-89	0.05548346357619849
1105	90-91	0.10112560254613356
1105	92-93	0.08440673310048297
1105	94-95	0.13140673810386616
1105	96-97	0.058514315448366716
1105	98-99	0.15828480998036198
1105	100	0.20556770881681175
1106	1	0.032216395738331016
1106	2	0.12540024831098862
1106	3	0.09305419793724923
1106	4	0.13436686663269626
1106	5	0.06089724518673023
1106	6	0.13585347502628053
1106	7	0.08643668340276633
1106	8	0.144601999873899
1106	9	0.16321407067624705
1106	10-11	0.1638676807507622
1106	12-13	0.18993660393062584
1106	14-15	0.2297591795562468
1106	16-17	0.231488626228888
1106	18-19	0.20537492956811576
1106	20-21	0.1816842033468049
1106	22-23	0.1815827116600559
1106	24-25	0.1938345392704477
1106	26-27	0.21385810297904584
1106	28-29	0.18516779823485763
1106	30-31	0.1586368830111482
1106	32-33	0.13996804327247503
1106	34-35	0.14126306309830028
1106	36-37	0.20057633749216564
1106	38-39	0.11749153864572293
1106	40-41	0.14340481662102889
1106	42-43	0.14012685084809817
1106	44-45	0.1795392143365575
1106	46-47	0.14784455598320534
1106	48-49	0.16503400732462126
1106	50-51	0.13942789981462766
1106	52-53	0.1554349211739705
1106	54-55	0.09627804221798897
1106	56-57	0.1290296911592108
1106	58-59	0.1257172454002955
1106	60-61	0.07370853433873492
1106	62-63	0.09942584668009147
1106	64-65	0.10077589767359285
1106	66-67	0.1688448489144463
1106	68-69	0.21037358748088764
1106	70-71	0.19598390414014943
1106	72-73	0.10976478313560989
1106	74-75	0.17038198958108808
1106	76-77	0.1599743073106552
1106	78-79	0.13101556358645183
1106	80-81	0.12650459555400317
1106	82-83	0.11416459159131165
1106	84-85	0.10689775392105005
1106	86-87	0.09442738783307192
1106	88-89	0.020071859362950306
1106	90-91	0.12530413627676396
1106	92-93	0.11518263201437762
1106	94-95	0.13429734751525402
1106	96-97	0.13972212163850983
1106	98-99	0.18979705662158963
1106	100	0.25692728462264824
1107	1	0.16614316333329526
1107	2	0.20241142707281767
1107	3	0.1444578602643105
1107	4	0.15540987414901508
1107	5	0.09816415307135173
1107	6	0.09768023278482474
1107	7	0.07676725174140842
1107	8	0.10327168068517523
1107	9	0.19974884299268325
1107	10-11	0.13128381942183154
1107	12-13	0.13257778741040482
1107	14-15	0.21984582541525555
1107	16-17	0.22687199020040083
1107	18-19	0.2018411090868213
1107	20-21	0.13946949030801647
1107	22-23	0.13193296460907078
1107	24-25	0.16814500362581697
1107	26-27	0.22234591318689922
1107	28-29	0.21413861765535813
1107	30-31	0.19630703973999175
1107	32-33	0.19975577696831692
1107	34-35	0.2211902677363824
1107	36-37	0.28200123940779775
1107	38-39	0.21199474961680664
1107	40-41	0.23021994149343783
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2216	80-81	-1.1188653190709417
2216	82-83	-1.1986519525719146
2216	84-85	-1.0696695062336836
2216	86-87	-1.1194076787572698
2216	88-89	-1.1222013680022478
2216	90-91	-1.197906068485544
2216	92-93	-1.1774303962029897
2216	94-95	-1.287260933651293
2216	96-97	-1.5614904296905543
2216	98-99	-1.6151869617304868
2216	100	-1.6684568897667695
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	363.0
3	814.0
4	516.0
5	486.0
6	885.0
7	1844.0
8	2999.0
9	4457.0
10	6117.0
11	8434.0
12	11661.0
13	16519.0
14	16840.0
15	20480.0
16	25492.0
17	35889.0
18	39812.0
19	22227.0
20	14484.0
21	12905.0
22	13098.0
23	13456.0
24	14925.0
25	17342.0
26	21387.0
27	26971.0
28	33683.0
29	42374.0
30	52564.0
31	65787.0
32	82291.0
33	104510.0
34	141003.0
35	222476.0
36	319410.0
37	600940.0
38	900368.0
39	450840.0
40	3003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.344775238881894	21.115042455851054	14.46999439147504	23.07018791379201
2	14.597471786404057	22.324827608310887	39.496185362761494	23.581515242523558
3	16.273000541005903	28.12174205681363	31.87293394653271	23.732323455647755
4	10.984282204019522	19.01524857258084	38.42993098852303	31.570538234876604
5	10.82541400096216	37.835676957124456	35.17851477535074	16.160394266562637
6	26.66818413296091	39.48654044987435	18.883077540351348	14.962197876813391
7	23.51201251642603	34.47807073252668	23.815575020803333	18.194341730243956
8	24.725461264249248	33.488027843824824	22.17638497981394	19.610125912111993
9	24.77154910952229	15.677612999799386	23.770911654972085	35.77992623570624
10-11	22.363414519770085	26.978522427033415	29.75295626783392	20.90510678536258
12-13	24.452614097835625	25.412920978190034	30.095674568174992	20.038790355799353
14-15	21.7429722713206	26.559953372039608	29.693526215763526	22.00354814087627
16-17	18.718639194789255	29.567370755199647	30.155888501245826	21.558101548765272
18-19	18.369834629807468	26.425340064790078	35.24739349938807	19.957431806014387
20-21	20.238944555698925	25.837623588429903	35.01828972249953	18.905142133371637
22-23	19.656673745538114	26.195598239818235	35.09236265347282	19.05536536117083
24-25	19.311509080891806	25.90399748577774	35.393346540518316	19.391146892812134
26-27	19.147882333249843	25.724392311134796	35.48885760309967	19.63886775251569
28-29	19.15230732294627	25.845869040648545	35.492134028521136	19.509689607884045
30-31	19.74660181468037	26.497639574075706	34.76887947508235	18.986879136161573
32-33	18.93366543547898	26.088215201240722	35.45151330436491	19.526606058915387
34-35	19.15801010267981	25.585566362308178	35.20090360846878	20.05551992654323
36-37	19.944731024865103	26.268640814430178	34.290195210210115	19.4964329504946
38-39	19.779149286422616	26.217942658883164	34.46715514111218	19.535752913582048
40-41	19.41706039405433	26.05441309777106	34.61216523032403	19.916361277850577
42-43	19.339410841044725	26.270238780288242	34.32260015556821	20.067750223098823
44-45	19.997584320279955	26.409655952602822	33.72547076077886	19.867288966338364
46-47	19.86637439001246	27.113279362163034	32.907239159334154	20.113107088490352
48-49	19.906823694624357	26.705248508480224	32.769023693526314	20.618904103369104
50-51	19.97853450593751	26.55597380574633	32.5203422133349	20.94514947498126
52-53	20.06108099894825	26.95147885936235	32.11595715130235	20.871482990387058
54-55	20.302691264999904	27.56869128577385	31.78976467568157	20.338852773544673
56-57	19.843134170663372	27.025989481931717	31.937681038985705	21.193195308419202
58-59	19.732874253620768	26.420737890206997	32.57306788743643	21.273319968735805
60-61	20.27539356139902	27.0009265156187	31.833171395892823	20.89050852708945
62-63	20.052662584042746	26.793473341514407	32.12476981450128	21.029094259941566
64-65	19.912678373652515	26.52029373114768	32.506428039786186	21.06059985541362
66-67	19.905512357822644	26.65577432888371	31.97327494541713	21.465438367876516
68-69	20.580411508515855	26.866761049036146	31.720548256609447	20.832279185838548
70-71	20.61612228845252	26.540509748891534	31.75100778584771	21.09236017680823
72-73	20.22147984420943	26.668673797769028	32.11407290723196	20.995773450789574
74-75	20.209488098514903	26.597524811608352	31.81019944994165	21.382787639935096
76-77	20.249464929909678	26.759128242322944	31.411463260894596	21.57994356687278
78-79	20.47647325391222	26.836023388897246	31.54214420383466	21.14535915335587
80-81	20.028670616431608	26.611472045184488	31.741111545049755	21.61874579333415
82-83	19.92699752968591	26.643245572487277	31.8271065036568	21.602650394170013
84-85	20.056371132460292	26.578120907843804	31.56540067796711	21.800107281728796
86-87	20.2342408058755	26.668436384528725	31.335223934103578	21.762098875492185
88-89	19.80458219424439	26.57688390373843	31.45407893752827	22.164454964488915
90-91	19.585316228500744	26.43962937419057	31.365390253949073	22.609664143359613
92-93	19.842731184254006	26.653716734806892	31.180796668609993	22.322755412329105
94-95	19.62573583266165	26.33789780072245	31.47158816400032	22.56477820261558
96-97	19.46521571005084	26.497972037954217	31.377381968810308	22.659430283184637
98-99	19.4734650706872	26.348751822898276	31.373481511641916	22.804301594772607
100	19.605649385201566	26.498371785006174	31.06345750185257	22.832521327939684
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4357.0
1	2681.5
2	8534.0
3	14845.5
4	8696.5
5	3903.0
6	6789.5
7	14897.5
8	22604.5
9	23473.5
10	15475.0
11	7246.0
12	5422.5
13	5435.0
14	5814.5
15	6197.5
16	6618.5
17	7164.5
18	7803.0
19	8549.5
20	9500.0
21	10807.5
22	12779.0
23	16026.5
24	19911.5
25	24726.5
26	31536.0
27	39905.5
28	50461.5
29	60986.5
30	68628.0
31	75881.5
32	82688.0
33	89063.5
34	95555.5
35	100410.5
36	103099.0
37	105003.5
38	106207.5
39	106349.5
40	106985.5
41	105839.0
42	102035.0
43	99440.0
44	98168.0
45	97228.0
46	96676.0
47	96622.5
48	97350.5
49	98297.5
50	99181.5
51	96175.0
52	89702.5
53	84110.0
54	80262.5
55	77416.5
56	71265.5
57	63478.5
58	58643.5
59	54580.0
60	47099.5
61	37935.0
62	30595.0
63	23611.5
64	17623.5
65	13025.0
66	9346.0
67	6763.5
68	4825.5
69	3301.5
70	2281.5
71	1634.5
72	1198.5
73	888.5
74	694.0
75	507.5
76	296.0
77	250.5
78	206.5
79	127.0
80	60.5
81	23.0
82	14.0
83	6.0
84	3.0
85	4.0
86	3.5
87	1.5
88	0.5
89	0.0
90	0.5
91	0.5
92	1.0
93	1.5
94	2.5
95	2.0
96	1.5
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04644396513349153
2	0.0
3	8.902996511212434E-5
4	0.07080849891917622
5	0.00459988153079309
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.010980362363828669
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.380480833035577E-4
26-27	0.0
28-29	3.2644320541112256E-4
30-31	0.003946995119970846
32-33	1.7805993022424867E-4
34-35	0.0015135094069061137
36-37	0.0038728034823774086
38-39	0.0013502878042005524
40-41	0.0012019045290136785
42-43	0.0020476891975788596
44-45	0.0
46-47	4.006348430045596E-4
48-49	5.490181181914335E-4
50-51	0.0017212459921677372
52-53	8.309463410464938E-4
54-55	0.0013948027867566146
56-57	0.006291450867923453
58-59	0.008977188148805871
60-61	0.0011277128914202418
62-63	0.0025818689882516057
64-65	0.0011870662014949911
66-67	1.483832751868739E-4
68-69	7.567547034530569E-4
70-71	3.7095818796718474E-4
72-73	0.0
74-75	2.8192822285506044E-4
76-77	0.0
78-79	5.638564457101209E-4
80-81	0.0
82-83	0.0011870662014949911
84-85	5.786947732288082E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.2257491278031085E-4
94-95	0.0
96-97	3.857965154858721E-4
98-99	5.34179790672746E-4
100	3.2644320541112256E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3369652.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.52429283121238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.04559114962899	47.40175511243638
2	15.12345428670388	18.609196603092474
3	4.140253538617449	7.641785133160894
4	1.5174860879496184	3.734490337692129
5	0.674227351318553	2.074068049866768
6	0.36281697936867296	1.3393234849688507
7	0.24242982619007122	1.0440726532276503
8	0.1570412871197808	0.7729483308278315
9	0.11804043037237531	0.6536118603737119
>10	0.5475699857218481	5.981574586497191
>50	0.040859818070472155	1.7313171803675604
>100	0.027285988670504177	3.2227861217711444
>500	0.0017852622964593464	0.7327018484391795
>1k	7.720053141900971E-4	0.6665027893778392
>5k	1.447509964106432E-4	0.6261599561886141
>10k+	2.4125166068440532E-4	3.76770595171189
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36004	1.0684782879656416	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31099	0.9229142950073183	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23741	0.7045534672423147	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23475	0.696659477002373	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12601	0.3739555301259596	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8306	0.24649429674043494	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6909	0.20503600965322233	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5878	0.17443937830968895	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.935331007474956E-5	0.0
5	0.0	0.0	0.0	5.935331007474956E-5	0.0
6	0.0	0.0	0.0	5.935331007474956E-5	0.0
7	0.0	0.0	0.0	5.935331007474956E-5	0.0
8	0.0	0.0	0.0	5.935331007474956E-5	0.0
9	0.0	0.0	0.0	5.935331007474956E-5	0.0
10-11	0.0	0.0	0.0	7.419163759343695E-5	0.0
12-13	0.0	0.0	0.0	8.902996511212434E-5	0.0
14-15	0.0	0.0	0.0	1.7805993022424867E-4	0.0
16-17	0.0	0.0	0.0	1.9289825774293607E-4	0.0
18-19	0.0	0.0	0.0	2.2257491278031085E-4	0.0
20-21	0.0	0.0	0.0	4.0063484300455947E-4	0.0
22-23	0.0	0.0	0.0	0.001216742856532366	0.0
24-25	0.0	0.0	0.0	0.002433485713064732	0.0
26-27	0.0	0.0	0.0	0.004421821600568842	0.0
28-29	0.0	0.0	0.0	0.009452014629403867	0.0
30-31	0.0	0.0	0.0	0.019913035530078477	0.0
32-33	0.0	0.0	0.0	0.03771902855250335	0.0
34-35	0.0	1.483832751868739E-5	0.0	0.06662409055890639	0.0
36-37	0.0	2.967665503737478E-5	0.0	0.11105004314985642	0.0
38-39	0.0	2.967665503737478E-5	0.0	0.17126397622068984	0.0
40-41	0.0	2.967665503737478E-5	0.0	0.24174603193445493	0.0
42-43	0.0	2.967665503737478E-5	0.0	0.3186085684812556	0.0
44-45	0.0	2.967665503737478E-5	0.0	0.4118377802811685	0.0
46-47	0.0	2.967665503737478E-5	0.0	0.5212556074039694	0.0
48-49	0.0	2.967665503737478E-5	0.0	0.6399622275534684	0.0
50-51	0.0	2.967665503737478E-5	0.0	0.762155854669859	0.0
52-53	2.967665503737478E-5	2.967665503737478E-5	0.0	0.8871242490322443	0.0
54-55	5.935331007474956E-5	2.967665503737478E-5	0.0	1.0328663019207918	0.0
56-57	5.935331007474956E-5	2.967665503737478E-5	0.0	1.204916709499972	0.0
58-59	5.935331007474956E-5	2.967665503737478E-5	0.0	1.3944466668961661	0.0
60-61	5.935331007474956E-5	2.967665503737478E-5	0.0	1.59347315390432	0.0
62-63	5.935331007474956E-5	2.967665503737478E-5	0.0	1.7984052952649117	0.0
64-65	5.935331007474956E-5	2.967665503737478E-5	0.0	2.0240220651865535	0.0
66-67	5.935331007474956E-5	2.967665503737478E-5	0.0	2.289242331255572	0.0
68-69	5.935331007474956E-5	2.967665503737478E-5	0.0	2.5824476830248346	0.0
70-71	5.935331007474956E-5	2.967665503737478E-5	0.0	2.899898861959633	0.0
72-73	5.935331007474956E-5	2.967665503737478E-5	0.0	3.2432280840870216	0.0
74-75	5.935331007474956E-5	2.967665503737478E-5	0.0	3.5901926964564885	0.0
76-77	5.935331007474956E-5	2.967665503737478E-5	0.0	3.96017155480744	0.0
78-79	5.935331007474956E-5	2.967665503737478E-5	0.0	4.382589062609433	0.0
80-81	8.902996511212434E-5	2.967665503737478E-5	0.0	4.841152736246948	0.0
82-83	8.902996511212434E-5	2.967665503737478E-5	0.0	5.325668644714647	0.0
84-85	8.902996511212434E-5	2.967665503737478E-5	0.0	5.808834265378145	0.0
86-87	8.902996511212434E-5	2.967665503737478E-5	0.0	6.323946805189379	0.0
88	8.902996511212434E-5	2.967665503737478E-5	0.0	6.733959471185749	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11625	0.0	63.389	1
TATAACG	315	0.0	49.27847	2
GTATCAA	21330	0.0	41.253983	1
ATAACGC	425	0.0	39.84441	3
GTGGTAT	3840	0.0	38.84572	1
TGGTATC	3855	0.0	37.826065	2
TACCTGG	2905	0.0	34.32761	2
GTACACG	325	0.0	31.8533	1
TAACGCA	555	0.0	31.359026	4
TATCAAC	28525	0.0	30.737894	2
ATCAACG	28510	0.0	30.737566	3
TCAACGC	28960	0.0	30.308674	4
CAACGCA	30065	0.0	29.226896	5
AACGCAG	31055	0.0	28.367922	6
GTACCTG	3580	0.0	28.259895	1
TCTGTCG	890	0.0	27.994667	8
TACACGG	365	0.0	27.06327	2
CGCAGAG	34575	0.0	25.43906	8
ACGCAGA	34735	0.0	25.34895	7
GCAGAGT	36365	0.0	23.928324	9
>>END_MODULE