FastQCFastQC Report
Wed 25 May 2016
SRR2431448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145508
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA325521.5172164354549134No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42270.19701627773002944No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24370.11358615302296704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG250.005236724456.399829
TACCTGG19700.040.803482
GGTATCA37050.040.0955851
GTACCTG21750.038.0409281
TATAACG1451.8189894E-1135.6608732
GTCTCGC7800.031.943241
TCTCGCT8950.029.4125272
CGCTCTG8350.029.2727785
TCTGTCG6950.029.0790428
GTATCAA57350.028.854061
TCGCTCT9850.028.6326874
CTCGCTC9900.025.6398683
ACCTGGG31450.024.8110223
GTACATG95600.024.3905431
ATCAACG64400.024.2331263
TCAACGC63900.024.201494
ATAACGC2253.1777745E-922.9809173
TACATGG94650.022.7463742
TATCAAC71850.022.6368852
CAACGCA68950.022.4289385
Produced by FastQC (version 0.11.5)