FastQCFastQC Report
Wed 25 May 2016
SRR2431448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145508
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235421.0972692714266272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206500.9624760196652726No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204320.9523152558741332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152230.7095289320757602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98990.46138257233251984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64020.2983908706003427No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53430.24903193089934877No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48500.22605368984874447No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84800.058.711691
TATAACG3000.054.8729932
ATAACGC3600.044.4209983
TACCTGG19800.042.2830852
GTATCAA152150.040.578561
GTACCTG22100.038.540831
GTGGTAT30800.034.9880331
TGGTATC31700.034.4223562
GCGTATC1309.085852E-932.5787121
GTATAAC5800.032.453891
ATCAACG199050.030.9779283
TATCAAC199500.030.8844782
TCAACGC201850.030.5715144
CAACGCA207950.029.6821175
ACCTGGG28250.028.8031183
AACGCAG217950.028.3829616
TAACGCA6500.026.0495954
CGCAGAG238600.025.7295068
ACGCAGA240600.025.5547037
TATCACG2251.1641532E-1025.0847992
Produced by FastQC (version 0.11.5)