##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431448_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2145508
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23195578855917	33.0	31.0	34.0	30.0	34.0
2	32.304073440882064	33.0	31.0	34.0	30.0	34.0
3	32.422189057323486	34.0	31.0	34.0	30.0	34.0
4	35.99452763634533	37.0	35.0	37.0	35.0	37.0
5	35.93277023436874	37.0	35.0	37.0	35.0	37.0
6	35.989344248541606	37.0	35.0	37.0	35.0	37.0
7	35.930371268715845	37.0	35.0	37.0	35.0	37.0
8	35.91303784465031	37.0	35.0	37.0	35.0	37.0
9	37.579290312597294	39.0	37.0	39.0	35.0	39.0
10-11	37.518932113047356	39.0	37.0	39.0	34.5	39.0
12-13	37.502301785870756	39.0	37.0	39.0	34.5	39.0
14-15	38.78673745332108	40.0	38.0	41.0	34.0	41.0
16-17	38.83850957442247	40.0	38.0	41.0	34.5	41.0
18-19	38.94330293804544	40.0	38.5	41.0	35.0	41.0
20-21	38.929818951968485	40.0	38.5	41.0	35.0	41.0
22-23	38.79653839556879	40.0	38.0	41.0	35.0	41.0
24-25	38.701759676496195	40.0	38.0	41.0	34.0	41.0
26-27	38.51400507478881	40.0	38.0	41.0	34.0	41.0
28-29	38.3770393771545	40.0	38.0	41.0	34.0	41.0
30-31	38.093876834763606	40.0	37.5	41.0	34.0	41.0
32-33	37.73832281212654	40.0	36.5	41.0	33.0	41.0
34-35	37.48151183775591	40.0	36.0	41.0	33.0	41.0
36-37	37.25460194042623	40.0	36.0	41.0	33.0	41.0
38-39	37.100946489129846	40.0	35.5	41.0	32.0	41.0
40-41	36.93106923861389	40.0	35.0	41.0	31.0	41.0
42-43	36.742455166795	40.0	35.0	41.0	31.0	41.0
44-45	36.43391192202499	40.0	35.0	41.0	30.0	41.0
46-47	35.966317767167496	40.0	35.0	41.0	28.0	41.0
48-49	35.58787545886568	40.0	35.0	41.0	26.0	41.0
50-51	35.40932403887564	40.0	35.0	41.0	24.5	41.0
52-53	35.25929406928336	40.0	35.0	41.0	24.0	41.0
54-55	35.08521478363166	39.0	35.0	41.0	24.0	41.0
56-57	34.865655592987764	39.0	35.0	41.0	23.0	41.0
58-59	34.695218102193046	39.0	35.0	41.0	23.0	41.0
60-61	34.46117469615588	38.5	35.0	41.0	23.0	41.0
62-63	34.19842922981411	38.0	34.5	40.5	22.0	41.0
64-65	33.93274553159438	37.0	34.0	40.0	22.0	41.0
66-67	33.659757502652056	37.0	34.0	40.0	21.0	41.0
68-69	33.34866567731278	36.0	34.0	39.5	20.0	41.0
70-71	32.99663925746257	36.0	34.0	39.0	18.5	41.0
72-73	32.66544217033915	35.0	33.0	39.0	18.0	41.0
74-75	32.364022180294825	35.0	33.0	38.5	17.0	40.0
76-77	32.024440132593305	35.0	33.0	37.0	13.0	39.5
78-79	31.72566963162104	35.0	33.0	37.0	11.0	39.0
80-81	31.439837558284566	35.0	33.0	36.0	9.0	39.0
82-83	31.204590940700292	35.0	33.0	36.0	7.0	38.0
84-85	30.990347041353374	35.0	33.0	36.0	3.5	37.0
86-87	30.759879012336473	35.0	33.0	35.0	2.0	37.0
88-89	30.618030321956386	35.0	33.0	35.0	2.0	36.5
90-91	30.44174876066647	35.0	33.0	35.0	2.0	36.0
92-93	30.244647188451406	35.0	32.0	35.0	2.0	36.0
94-95	30.07751124675368	35.0	32.0	35.0	2.0	36.0
96-97	29.907013164248283	35.0	32.0	35.0	2.0	35.5
98-99	29.740701968950944	35.0	32.0	35.0	2.0	35.0
100	29.62814447673931	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10507791473532535
1101	2	0.18947931466066592
1101	3	0.19828566606835807
1101	4	0.14957305631710227
1101	5	0.11522771676729349
1101	6	0.19301349256860334
1101	7	0.1577426589818245
1101	8	0.2068328191552311
1101	9	0.25104404722862483
1101	10-11	0.27111335330766906
1101	12-13	0.2595398049375319
1101	14-15	0.38459767632777186
1101	16-17	0.33748757589178524
1101	18-19	0.3020477680538818
1101	20-21	0.30123760090845053
1101	22-23	0.3388979686124287
1101	24-25	0.3083060324118705
1101	26-27	0.27542015428687705
1101	28-29	0.283078165416768
1101	30-31	0.2430065516712574
1101	32-33	0.34374423249005304
1101	34-35	0.2679070693887624
1101	36-37	0.36477204601706603
1101	38-39	0.3534925912822686
1101	40-41	0.4581576395362532
1101	42-43	0.4232127045027525
1101	44-45	0.48607041621843194
1101	46-47	0.32773035681052676
1101	48-49	0.29800102032191944
1101	50-51	0.23560451089569767
1101	52-53	0.23286878452069004
1101	54-55	0.15707872402182232
1101	56-57	0.1347920611862392
1101	58-59	0.21651353601539824
1101	60-61	0.1934076201580126
1101	62-63	0.19854314394426353
1101	64-65	0.21700611221760369
1101	66-67	0.17542247780871634
1101	68-69	0.1383646437077104
1101	70-71	0.17932770606377346
1101	72-73	0.1863757108373747
1101	74-75	0.17465608317898074
1101	76-77	0.16209273762068932
1101	78-79	0.10153373548308764
1101	80-81	0.16408033675594424
1101	82-83	0.13860313101515231
1101	84-85	0.08215331568867867
1101	86-87	0.11407912782919283
1101	88-89	0.07269456331123081
1101	90-91	0.16707548127380534
1101	92-93	0.18154158484987448
1101	94-95	0.16140414818549687
1101	96-97	0.17634329776869606
1101	98-99	0.1976059282515763
1101	100	0.28357633158428897
1102	1	0.1201092215521058
1102	2	0.22264053587019106
1102	3	0.20513649021410885
1102	4	0.15398386866682756
1102	5	0.10668807215741083
1102	6	0.1146047805625301
1102	7	0.1638319782152493
1102	8	0.19116252369494902
1102	9	0.16100263650309188
1102	10-11	0.20543936309220356
1102	12-13	0.21147704668717182
1102	14-15	0.35771306217242227
1102	16-17	0.32350916096896754
1102	18-19	0.2976440958752278
1102	20-21	0.27408081023175157
1102	22-23	0.3121355873857752
1102	24-25	0.2589734570700344
1102	26-27	0.2608422483335886
1102	28-29	0.26383376098750944
1102	30-31	0.2322505072897627
1102	32-33	0.22712880505108046
1102	34-35	0.23632223928289164
1102	36-37	0.16231243841820486
1102	38-39	0.2118810682701806
1102	40-41	0.26513597807057465
1102	42-43	0.2091016977363651
1102	44-45	0.029832014746332902
1102	46-47	0.10082988694193062
1102	48-49	-0.04035570670526312
1102	50-51	0.03267960214821386
1102	52-53	0.14097313936769496
1102	54-55	0.1586399280949422
1102	56-57	0.20804143290785504
1102	58-59	0.04763505968710291
1102	60-61	0.011615351081914582
1102	62-63	0.020197027437980353
1102	64-65	0.08280370672796522
1102	66-67	0.05016069986984917
1102	68-69	0.04196264391580229
1102	70-71	-0.0592311898073703
1102	72-73	-0.05077730801562552
1102	74-75	-0.019658629268199945
1102	76-77	0.04777089180260674
1102	78-79	0.09823117968718265
1102	80-81	0.04419867772472941
1102	82-83	0.05701296278138557
1102	84-85	0.006139281059077462
1102	86-87	0.03617391122020308
1102	88-89	0.034708796505590556
1102	90-91	0.07777279491477174
1102	92-93	0.015312225327754447
1102	94-95	-0.04140272106709375
1102	96-97	-0.08739892482828893
1102	98-99	-0.07388712005519693
1102	100	-0.12420631888801736
1103	1	0.10407606233749789
1103	2	0.15145576195076416
1103	3	0.1656220515006126
1103	4	0.21682255149697482
1103	5	0.07666146155916209
1103	6	0.07295185722952624
1103	7	0.15005403982480203
1103	8	0.10553224674761452
1103	9	0.21473326716331798
1103	10-11	0.21722303973510293
1103	12-13	0.23620398690641053
1103	14-15	0.27046282399113863
1103	16-17	0.3017834143938529
1103	18-19	0.24083386338840285
1103	20-21	0.23147362865547905
1103	22-23	0.23315303211128935
1103	24-25	0.2307526438589136
1103	26-27	0.2641082338565468
1103	28-29	0.2134749011914039
1103	30-31	0.10087212210409291
1103	32-33	0.12715826287382725
1103	34-35	0.11563156832348653
1103	36-37	0.1752610679176385
1103	38-39	0.09785991120194382
1103	40-41	0.18506446893208306
1103	42-43	0.14985741682481546
1103	44-45	0.18168457232049917
1103	46-47	0.18034618730222007
1103	48-49	0.1850074786819036
1103	50-51	0.1146321411059148
1103	52-53	0.15768941344590814
1103	54-55	0.2205488826160078
1103	56-57	0.13219553345867752
1103	58-59	0.1686755012259198
1103	60-61	0.18558087776369092
1103	62-63	0.13363395593979277
1103	64-65	0.20047938595526915
1103	66-67	0.25442507561587746
1103	68-69	0.22778116620627742
1103	70-71	0.36930489876321104
1103	72-73	0.23305012827773197
1103	74-75	0.2441980458306361
1103	76-77	0.18053850352878698
1103	78-79	0.2590332209281314
1103	80-81	0.2599167805971412
1103	82-83	0.23136253624525338
1103	84-85	0.30042774870474886
1103	86-87	0.32266952539937677
1103	88-89	0.2610438710262173
1103	90-91	0.19078778664686524
1103	92-93	0.1345751227072114
1103	94-95	0.22433761205817504
1103	96-97	0.1701182954045244
1103	98-99	0.16511503726490773
1103	100	0.23439021844895436
1104	1	0.13819059303398262
1104	2	0.15277638619944867
1104	3	0.17372324191348554
1104	4	0.1257201164164954
1104	5	0.15436616682825388
1104	6	0.18939593761164986
1104	7	0.09100126379050977
1104	8	0.12344694978268933
1104	9	0.23556751360750638
1104	10-11	0.24202703118200475
1104	12-13	0.25869076896191956
1104	14-15	0.38982368185435234
1104	16-17	0.3279404765527616
1104	18-19	0.30626635878738284
1104	20-21	0.24186745425308231
1104	22-23	0.21267027128959626
1104	24-25	0.3130057049965629
1104	26-27	0.37125634930212925
1104	28-29	0.37317650011056713
1104	30-31	0.3607735061319204
1104	32-33	0.31181353249862553
1104	34-35	0.3442682849541683
1104	36-37	0.2581188034736499
1104	38-39	0.22625439636698985
1104	40-41	0.37823975430139
1104	42-43	0.34094346910084994
1104	44-45	0.3628325388314906
1104	46-47	0.31649558996814875
1104	48-49	0.21355802601650709
1104	50-51	0.19203844529415903
1104	52-53	0.21522135459547087
1104	54-55	0.23204056887465896
1104	56-57	0.20794270636110213
1104	58-59	0.26666754386376823
1104	60-61	0.23169708929336252
1104	62-63	0.3099814416048403
1104	64-65	0.23337935510453178
1104	66-67	0.2573114278887232
1104	68-69	0.23006922697158672
1104	70-71	0.25811310293124734
1104	72-73	0.1860313731581016
1104	74-75	0.16391379353544977
1104	76-77	0.1070252430315648
1104	78-79	0.10793770066771202
1104	80-81	0.10674351648292912
1104	82-83	0.09793853011765918
1104	84-85	0.08836223876336646
1104	86-87	0.07431833764605855
1104	88-89	-0.056000309354843125
1104	90-91	-0.0327930263232048
1104	92-93	0.009901555404734808
1104	94-95	0.0025199718203055
1104	96-97	0.08355698222700525
1104	98-99	0.1382442592944635
1104	100	0.23515744432953056
1105	1	0.13716110595501618
1105	2	0.19228913040286955
1105	3	0.1438113978700386
1105	4	0.15187017255137647
1105	5	0.08630282866270989
1105	6	0.17221078090940978
1105	7	0.11894421640198516
1105	8	0.18891123448413794
1105	9	0.21088818308897572
1105	10-11	0.166086771877211
1105	12-13	0.10030729930895887
1105	14-15	0.19844272200622726
1105	16-17	0.22387126048934647
1105	18-19	0.317544007094412
1105	20-21	0.2624534423855138
1105	22-23	0.23277216903235853
1105	24-25	0.2246095278405349
1105	26-27	0.2803486991100357
1105	28-29	0.32179973578199395
1105	30-31	0.3322144669142375
1105	32-33	0.25776827593801244
1105	34-35	0.23646240822403541
1105	36-37	0.26784408869390575
1105	38-39	0.21670562741416433
1105	40-41	0.2247466089295571
1105	42-43	0.2758765284603655
1105	44-45	0.20493607891580012
1105	46-47	0.2478467140837921
1105	48-49	0.19514517938568332
1105	50-51	0.26138368984373983
1105	52-53	0.23994593500181338
1105	54-55	0.2607047046059776
1105	56-57	0.21699904393110359
1105	58-59	0.19831592012263144
1105	60-61	0.1859129608687553
1105	62-63	0.1870279042730587
1105	64-65	0.22074024819134763
1105	66-67	0.28129220023132717
1105	68-69	0.18368986442364843
1105	70-71	0.18191562825033714
1105	72-73	0.12763628867218557
1105	74-75	0.15706521258861272
1105	76-77	0.21565249906168305
1105	78-79	0.20861411350683312
1105	80-81	0.1659964721138465
1105	82-83	0.13285079955175405
1105	84-85	0.14004730705107704
1105	86-87	0.14330398112865694
1105	88-89	0.22102669794686136
1105	90-91	0.28529377088556274
1105	92-93	0.12454214618059822
1105	94-95	0.18202356595425329
1105	96-97	0.11310630585586878
1105	98-99	0.2098353162484301
1105	100	0.2788813474472249
1106	1	0.08929598387759796
1106	2	0.21199822642298471
1106	3	0.1134986979894066
1106	4	0.20375144396533784
1106	5	0.06566720316109809
1106	6	0.15157179435768597
1106	7	0.14027720887730766
1106	8	0.17761403210003124
1106	9	0.18854636632935495
1106	10-11	0.2025520776920331
1106	12-13	0.18033975890505616
1106	14-15	0.25414316485004207
1106	16-17	0.24995285660111932
1106	18-19	0.232341488547533
1106	20-21	0.25399785509802086
1106	22-23	0.21026846169223035
1106	24-25	0.29971473889411726
1106	26-27	0.2551017128516406
1106	28-29	0.24744414075389898
1106	30-31	0.2217040048818859
1106	32-33	0.18325267455448113
1106	34-35	0.16176001393414907
1106	36-37	0.2115931975063745
1106	38-39	0.2990409665135658
1106	40-41	0.2508128256908009
1106	42-43	0.31676864282569994
1106	44-45	0.31550780372523946
1106	46-47	0.2882137407562695
1106	48-49	0.28736725109623507
1106	50-51	0.29337574350647344
1106	52-53	0.24741714200582976
1106	54-55	0.33779655090209815
1106	56-57	0.39791505230724766
1106	58-59	0.3402454786232383
1106	60-61	0.266456147307224
1106	62-63	0.2017976825252319
1106	64-65	0.2943111907927687
1106	66-67	0.30371244149361587
1106	68-69	0.26322529600285094
1106	70-71	0.27806305001934817
1106	72-73	0.3453397544240957
1106	74-75	0.4509255925298632
1106	76-77	0.43725773659625844
1106	78-79	0.3830276611868939
1106	80-81	0.3120720896304192
1106	82-83	0.23469214576436315
1106	84-85	0.2334505332517871
1106	86-87	0.29559020815638704
1106	88-89	0.30615909276636444
1106	90-91	0.36911596726051954
1106	92-93	0.4448206346450654
1106	94-95	0.38895323648986135
1106	96-97	0.4374755997094262
1106	98-99	0.40947267931110076
1106	100	0.5080261333381486
1107	1	0.07740525055120884
1107	2	0.11598071607642169
1107	3	0.10093270799657716
1107	4	0.11695266781307367
1107	5	0.03099366762216249
1107	6	0.07394405732759424
1107	7	0.08249384712728869
1107	8	0.03222176212246808
1107	9	0.09646653377033232
1107	10-11	0.11112971388320148
1107	12-13	0.1153544901340453
1107	14-15	0.14439265014886615
1107	16-17	0.18415892179187665
1107	18-19	0.1593598184087952
1107	20-21	0.12571716959864432
1107	22-23	0.14009029420923014
1107	24-25	0.14891059313680444
1107	26-27	0.14418633397635006
1107	28-29	0.18945357910044436
1107	30-31	0.18856677843828606
1107	32-33	0.19261420075769564
1107	34-35	0.22921335608325677
1107	36-37	0.0795976718514595
1107	38-39	-0.011742713366487578
1107	40-41	0.17321377411904848
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2216	62-63	-1.347609840119766
2216	64-65	-1.3423962344387803
2216	66-67	-1.209866869467163
2216	68-69	-1.0482294046636653
2216	70-71	-1.0837851470009205
2216	72-73	-1.0655657752953118
2216	74-75	-1.0297582956170572
2216	76-77	-0.9912866903779225
2216	78-79	-0.8877061383740141
2216	80-81	-1.020384661080449
2216	82-83	-1.0236364969068887
2216	84-85	-1.0901908190712852
2216	86-87	-1.0512004443060547
2216	88-89	-1.0371300756467505
2216	90-91	-1.077087296609477
2216	92-93	-0.9553872776537453
2216	94-95	-1.0928323168247935
2216	96-97	-1.2557148051691378
2216	98-99	-1.3638171329862558
2216	100	-1.359753691483796
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	155.0
3	408.0
4	312.0
5	337.0
6	671.0
7	1382.0
8	2387.0
9	3687.0
10	4955.0
11	6768.0
12	9836.0
13	14023.0
14	12889.0
15	14677.0
16	18354.0
17	26407.0
18	29236.0
19	15792.0
20	9867.0
21	8853.0
22	8571.0
23	9101.0
24	10097.0
25	11698.0
26	14163.0
27	17709.0
28	21764.0
29	27271.0
30	33699.0
31	41954.0
32	52569.0
33	67203.0
34	92188.0
35	145635.0
36	205122.0
37	391226.0
38	565317.0
39	247312.0
40	1913.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31630200155842	20.171892566912522	13.496191848929273	23.015613582599784
2	15.47237297646991	21.94557186456541	39.08244574245354	23.49960941651115
3	16.519148880635974	27.98992684236431	31.343591062985666	24.14733321401405
4	11.17198289392001	18.83007397740613	37.93570341501273	32.06223971366112
5	10.65785658951945	36.979810964929044	34.74649742568129	17.615835019870214
6	26.469628638066133	38.791419095151355	19.161709021826066	15.57724324495644
7	23.447686981358263	33.90917209350886	24.070336722118956	18.572804203013927
8	24.611607134534104	32.195871560488236	22.99520672959504	20.197314575382613
9	24.54309189245624	15.492181804961808	24.500071777872652	35.464654524709296
10-11	22.176180318729408	26.501187730390797	29.779053246494147	21.543578704385652
12-13	24.673014502858997	24.966884299662365	30.03333010177543	20.326771095703208
14-15	22.108213998735966	26.128054521353448	29.947709353682207	21.81602212622838
16-17	18.687602190250512	29.052816395930474	30.27369275714656	21.985888656672454
18-19	18.29002735016602	25.533393490026608	35.82132996008405	20.35524919972333
20-21	20.328751978552397	25.211208720731875	35.39413509527813	19.065904205437594
22-23	19.668558681673524	25.70421550513911	35.58597777309616	19.041248040091205
24-25	19.23256817137875	25.256590150471208	35.9888995235828	19.52194215456724
26-27	18.96189620360306	24.932463547094674	36.17182970187014	19.933810547432124
28-29	19.194342396324963	25.102866368803202	36.06221294596789	19.64057828890395
30-31	19.92873646475254	26.095835914416636	35.113243719053216	18.862183901777605
32-33	18.73056112145055	25.31849115383916	36.202794957089395	19.748152767620894
34-35	18.968411073206905	24.612520257827317	35.91814742431368	20.500921244652094
36-37	20.298380499810058	25.795661911564892	34.45242528834748	19.45353230027757
38-39	19.98315530030082	25.632585125393735	34.80224956443354	19.582010009871908
40-41	19.26540015968447	25.418216241486174	35.04712929175323	20.269254307076128
42-43	19.194187767277644	25.615679511530082	34.75237418287326	20.43775853831902
44-45	20.056904938131204	25.870096965380696	34.03748669312815	20.03551140335995
46-47	19.90257278143686	26.90122437717286	32.922970367266544	20.273232474123734
48-49	19.861211903420227	26.153448541855724	33.11988668380952	20.865452870914535
50-51	20.00812872469445	25.941165558444318	32.68481429313188	21.365891423729348
52-53	20.124707992385897	26.44761667720038	32.146938790729585	21.280736539684135
54-55	20.52035629132066	27.35522373311663	31.696233706821957	20.428186268740752
56-57	19.867049441964703	26.63928695282523	31.871124236966438	21.622539368243636
58-59	19.63724360516174	25.76095731926739	32.72414796086745	21.87765111470342
60-61	20.602211941744788	26.622047499161617	31.693172260608936	21.08256829848466
62-63	20.238689792993352	26.42856726696458	32.00845971439725	21.32428322564482
64-65	20.03833156101134	26.097785098559044	32.52025585757662	21.343627482852987
66-67	20.06373783358949	26.263436506281145	31.867215565548012	21.80561009458135
68-69	20.981646905506487	26.599647214525866	31.539040031060413	20.879665848907234
70-71	21.131626617422615	26.07242259338072	31.613893142615172	21.18205764658149
72-73	20.47645592558965	26.250822648995015	32.24723468754253	21.025486737872804
74-75	20.428221360730177	26.16436009745969	31.94508046483283	21.462338076977304
76-77	20.603488777483	26.52346204255589	31.27753427160374	21.59551490835737
78-79	20.909528824460363	26.678033116359366	31.41826678266163	20.994171276518642
80-81	20.445973634216234	26.28284303763957	31.68687788626283	21.584305441881362
82-83	20.061212309486418	26.348396526936703	31.939164295600502	21.651226867976376
84-85	20.337684704094748	26.3483559136189	31.5181013217245	21.79585806056185
86-87	20.631948385172272	26.506898717436318	31.2457309051588	21.615421992232605
88-89	19.98002337907852	26.401020177971834	31.551152454337156	22.067803988612486
90-91	19.755577700013237	26.198993431858565	31.408901761261205	22.636527106867
92-93	20.090622084746073	26.68781477168413	31.078392554474977	22.143170589094822
94-95	19.96419962078911	26.226958836788306	31.525750544859303	22.28309099756328
96-97	19.75093002731529	26.37807524254819	31.523865166255998	22.347129563880518
98-99	19.781057722289987	26.222152722655867	31.414888951138124	22.581900603916015
100	20.10986695899891	26.47736848777723	30.949231509239787	22.46353304398407
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4816.0
1	2936.0
2	7606.0
3	12520.0
4	6958.5
5	2964.0
6	4645.5
7	9755.0
8	14398.0
9	14863.0
10	10014.0
11	4822.0
12	3667.5
13	3695.5
14	3926.0
15	4267.5
16	4481.5
17	4829.0
18	5319.5
19	5680.5
20	6155.5
21	6963.0
22	8064.5
23	10129.0
24	12510.0
25	14669.5
26	18158.5
27	22897.0
28	28991.0
29	34457.0
30	37625.5
31	41218.0
32	45808.5
33	50154.5
34	54335.0
35	58061.0
36	60614.5
37	62877.0
38	65114.0
39	65553.0
40	66654.5
41	67124.5
42	65156.0
43	65414.0
44	65475.5
45	63880.0
46	64467.5
47	66143.5
48	67582.0
49	68527.5
50	69762.5
51	67419.0
52	60237.0
53	54582.0
54	51414.5
55	49267.5
56	45886.5
57	42109.5
58	39338.5
59	36447.0
60	31283.0
61	25361.0
62	20650.5
63	15780.5
64	11572.0
65	8613.0
66	6338.0
67	4510.5
68	3099.0
69	2086.0
70	1421.5
71	968.0
72	721.5
73	540.5
74	446.5
75	376.5
76	250.0
77	190.0
78	137.5
79	76.5
80	40.0
81	12.5
82	8.5
83	5.0
84	3.0
85	2.5
86	2.0
87	2.0
88	1.5
89	0.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.5
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04796066945450681
2	0.0
3	1.8643603286494387E-4
4	0.06874828711894806
5	0.004008374706596293
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.011186161971896633
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.758306191354215E-4
26-27	0.0
28-29	2.796540492974158E-4
30-31	0.004241419747677473
32-33	4.660900821623597E-5
34-35	0.0019109693368656747
36-37	0.004334637764109945
38-39	0.0012118342136221351
40-41	0.0012584432218383712
42-43	0.001538097271135787
44-45	0.0
46-47	4.194810739461238E-4
48-49	3.262630575136518E-4
50-51	0.001398270246487079
52-53	7.457441314597755E-4
54-55	7.690486355678935E-4
56-57	0.00577951701881326
58-59	0.00857605751178742
60-61	8.156576437841295E-4
62-63	0.0021207098738387363
64-65	8.156576437841295E-4
66-67	2.3304504108117984E-5
68-69	5.360035944867137E-4
70-71	1.631315287568259E-4
72-73	0.0
74-75	2.5634954518929785E-4
76-77	0.0
78-79	5.826126027029495E-4
80-81	0.0
82-83	0.0011419207012977812
84-85	7.224396273516575E-4
86-87	2.3304504108117984E-5
88-89	0.0
90-91	0.0
92-93	2.097405369730619E-4
94-95	0.0
96-97	3.029585534055338E-4
98-99	5.126990903785957E-4
100	1.8643603286494387E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2145508.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.29203746294525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.14368891481395	52.811851250557396
2	12.651527021381808	16.267848984442868
3	2.7411637541669536	5.287050083249082
4	0.8812765272731313	2.2663625402663383
5	0.3933970474422078	1.264614885598324
6	0.22155733806187125	0.8546623607318553
7	0.15125677114522734	0.6807224197895164
8	0.10406073625152243	0.5352221402803635
9	0.0787141770940693	0.4554625340337129
>10	0.5354353749817367	6.81974498233542
>50	0.058888683130791354	2.64761316060527
>100	0.03649116268364746	4.123474700337563
>500	0.0016707801766219412	0.7500568952287106
>1k	3.6321308187432295E-4	0.49533645272699317
>5k	2.1792784912459377E-4	1.0108487820877843
>10k+	2.9057046549945835E-4	3.7291278277288535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23542	1.0972692714266272	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20650	0.9624760196652726	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20432	0.9523152558741332	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15223	0.7095289320757602	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9899	0.46138257233251984	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6402	0.2983908706003427	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5343	0.24903193089934877	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4850	0.22605368984874447	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	2.3304504108117984E-5	0.0
16-17	0.0	0.0	0.0	4.660900821623597E-5	0.0
18-19	0.0	0.0	0.0	6.991351232435395E-5	0.0
20-21	0.0	0.0	0.0	2.796540492974158E-4	0.0
22-23	2.3304504108117984E-5	0.0	0.0	0.0011419207012977812	0.0
24-25	4.660900821623597E-5	0.0	0.0	0.0031694125587040456	0.0
26-27	4.660900821623597E-5	0.0	0.0	0.005080381895569721	0.0
28-29	4.660900821623597E-5	0.0	0.0	0.009415019659679665	0.0
30-31	4.660900821623597E-5	0.0	0.0	0.0194359564261704	0.0
32-33	4.660900821623597E-5	0.0	0.0	0.03819608223320538	0.0
34-35	4.660900821623597E-5	0.0	0.0	0.0650428709657573	0.0
36-37	4.660900821623597E-5	0.0	0.0	0.10200381448123241	0.0
38-39	4.660900821623597E-5	0.0	0.0	0.15672279012709345	0.0
40-41	4.660900821623597E-5	0.0	0.0	0.22360671691739206	0.0
42-43	4.660900821623597E-5	0.0	0.0	0.2922384815157995	0.0
44-45	4.660900821623597E-5	0.0	0.0	0.3709144873848058	0.0
46-47	4.660900821623597E-5	0.0	0.0	0.4596114300203029	0.0
48-49	4.660900821623597E-5	0.0	0.0	0.556022163515587	0.0
50-51	4.660900821623597E-5	0.0	0.0	0.6580492825009274	0.0
52-53	4.660900821623597E-5	0.0	0.0	0.7558349817385905	0.0
54-55	4.660900821623597E-5	0.0	0.0	0.8679529510027462	0.0
56-57	4.660900821623597E-5	0.0	0.0	1.0007886244190187	0.0
58-59	4.660900821623597E-5	0.0	0.0	1.1477468273248106	0.0
60-61	4.660900821623597E-5	0.0	0.0	1.2943088536607648	0.0
62-63	4.660900821623597E-5	0.0	0.0	1.4411971430542323	0.0
64-65	4.660900821623597E-5	0.0	0.0	1.6100848843257634	0.0
66-67	4.660900821623597E-5	0.0	0.0	1.8082896917653069	0.0
68-69	4.660900821623597E-5	0.0	0.0	2.022411475510695	0.0
70-71	4.660900821623597E-5	0.0	0.0	2.25650521927674	0.0
72-73	4.660900821623597E-5	0.0	0.0	2.51679322566031	0.0
74-75	4.660900821623597E-5	0.0	0.0	2.7779434986958798	0.0
76-77	4.660900821623597E-5	0.0	0.0	3.047623220235021	0.0
78-79	4.660900821623597E-5	0.0	0.0	3.351164386243258	0.0
80-81	4.660900821623597E-5	0.0	0.0	3.6812027734224246	0.0
82-83	4.660900821623597E-5	0.0	0.0	4.0364566340465755	0.0
84-85	4.660900821623597E-5	0.0	0.0	4.394343903634944	0.0
86-87	4.660900821623597E-5	0.0	0.0	4.7757687223725105	0.0
88	4.660900821623597E-5	0.0	0.0	5.084763142342046	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8480	0.0	58.71169	1
TATAACG	300	0.0	54.872993	2
ATAACGC	360	0.0	44.420998	3
TACCTGG	1980	0.0	42.283085	2
GTATCAA	15215	0.0	40.57856	1
GTACCTG	2210	0.0	38.54083	1
GTGGTAT	3080	0.0	34.988033	1
TGGTATC	3170	0.0	34.422356	2
GCGTATC	130	9.085852E-9	32.578712	1
GTATAAC	580	0.0	32.45389	1
ATCAACG	19905	0.0	30.977928	3
TATCAAC	19950	0.0	30.884478	2
TCAACGC	20185	0.0	30.571514	4
CAACGCA	20795	0.0	29.682117	5
ACCTGGG	2825	0.0	28.803118	3
AACGCAG	21795	0.0	28.382961	6
TAACGCA	650	0.0	26.049595	4
CGCAGAG	23860	0.0	25.729506	8
ACGCAGA	24060	0.0	25.554703	7
TATCACG	225	1.1641532E-10	25.084799	2
>>END_MODULE