FastQCFastQC Report
Wed 25 May 2016
SRR2431447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6224420
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA353530.5679725982501181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89350.047.1397321
GTATCAA159600.038.2604371
ATCAACG189700.031.0234243
TCAACGC190350.030.8927924
TATCAAC205350.029.094712
CAACGCA215050.027.3659555
GTACATG353800.026.0019951
AACGCAG227850.025.7648586
TACATGG351400.024.9750162
CATGGGT158650.024.4733094
CGCAGAG237000.024.0357748
ACGCAGA240550.023.896377
ACATGGG365250.023.306713
GCAGAGT253100.021.3740629
ATGGGTG97100.020.815895
GTGGTAT49100.020.2968221
TGGTATC46600.019.7712142
ATGGGTA67800.018.441565
AGAGTAC237700.018.25020210-11
CAGAGTA251300.017.786210-11
Produced by FastQC (version 0.11.5)