##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431447_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6224420
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.873299841591667	31.0	30.0	34.0	26.0	34.0
2	29.943157113433863	31.0	30.0	34.0	25.0	34.0
3	30.015172016027197	31.0	31.0	34.0	25.0	34.0
4	33.3047008395963	37.0	35.0	37.0	26.0	37.0
5	33.40671387856218	37.0	35.0	37.0	27.0	37.0
6	33.45810469087883	37.0	35.0	37.0	27.0	37.0
7	33.315513573955485	37.0	35.0	37.0	26.0	37.0
8	33.24791016673039	37.0	35.0	37.0	26.0	37.0
9	34.51047647812969	39.0	35.0	39.0	24.0	39.0
10-11	34.480438498687434	39.0	35.0	39.0	25.0	39.0
12-13	34.43224027298929	39.0	35.0	39.0	24.5	39.0
14-15	35.58088584960527	40.0	36.0	41.0	24.5	41.0
16-17	35.6098866239746	40.0	36.0	41.0	25.0	41.0
18-19	35.62326875435784	40.0	36.0	41.0	25.0	41.0
20-21	35.741479366752245	40.0	36.0	41.0	25.0	41.0
22-23	35.71076236822066	40.0	36.0	41.0	25.0	41.0
24-25	35.75964507536445	40.0	36.0	41.0	26.0	41.0
26-27	35.76928621461919	40.0	36.0	41.0	27.0	41.0
28-29	35.670089662972615	40.0	35.5	41.0	26.5	41.0
30-31	35.65181575150777	40.0	35.0	41.0	27.0	41.0
32-33	35.605595541431974	40.0	35.0	41.0	26.5	41.0
34-35	35.5044113347107	40.0	35.0	41.0	25.5	41.0
36-37	35.38047834175714	40.0	35.0	41.0	25.0	41.0
38-39	35.27763100819032	40.0	35.0	41.0	25.0	41.0
40-41	35.19153254439771	40.0	35.0	41.0	25.0	41.0
42-43	34.968403240783886	39.5	34.5	41.0	24.0	41.0
44-45	34.83197382246057	39.0	34.0	41.0	24.0	41.0
46-47	34.695707873183366	39.0	34.0	41.0	24.0	41.0
48-49	34.565896581528875	39.0	34.0	41.0	23.5	41.0
50-51	34.39707980823916	39.0	33.5	41.0	23.0	41.0
52-53	34.30372580899104	39.0	33.0	40.5	23.0	41.0
54-55	34.23766037638848	38.5	33.0	41.0	23.0	41.0
56-57	34.07620702009183	38.0	33.0	40.0	22.0	41.0
58-59	33.87310608859942	38.0	33.0	40.0	21.5	41.0
60-61	33.65916880608957	38.0	33.0	40.0	20.5	41.0
62-63	33.39670820735105	37.0	33.0	40.0	19.5	41.0
64-65	33.261269724729374	37.0	33.0	40.0	18.5	41.0
66-67	33.0133362626558	36.5	32.0	40.0	18.0	41.0
68-69	32.75632123153643	36.0	32.0	39.5	15.5	41.0
70-71	32.4742429816754	36.0	32.0	39.0	13.0	41.0
72-73	32.1707830609117	35.0	32.0	39.0	10.0	41.0
74-75	31.834965828141417	35.0	31.5	38.5	7.5	40.0
76-77	31.514641926476685	35.0	31.0	37.0	6.5	40.0
78-79	31.18403513901697	35.0	31.0	37.0	2.0	39.0
80-81	30.890925821201012	35.0	31.0	36.5	2.0	39.0
82-83	30.59246676798802	35.0	31.0	36.0	2.0	38.5
84-85	30.307307990142053	35.0	31.0	36.0	2.0	37.0
86-87	30.057208302138996	35.0	30.0	35.0	2.0	37.0
88-89	29.800279945762014	35.0	30.0	35.0	2.0	36.5
90-91	29.5877451232404	34.5	30.0	35.0	2.0	36.0
92-93	29.411433113446716	34.0	30.0	35.0	2.0	36.0
94-95	29.197135636734025	34.0	30.0	35.0	2.0	36.0
96-97	29.114163327667477	34.0	30.0	35.0	2.0	35.5
98-99	28.974692501470017	34.0	30.0	35.0	2.0	35.0
100	28.846390025094706	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3295386160611997
1101	2	0.4658810795683408
1101	3	0.37586334814210787
1101	4	0.38420264776453905
1101	5	0.3169960407782284
1101	6	0.36577011243608126
1101	7	0.38879633331408314
1101	8	0.5185028621460717
1101	9	0.6436550953797067
1101	10-11	0.7112546190920881
1101	12-13	0.6110714841561062
1101	14-15	0.7008893914374923
1101	16-17	0.686930646777931
1101	18-19	0.6017782352898848
1101	20-21	0.5721273713094419
1101	22-23	0.5289785646672556
1101	24-25	0.5768741170820277
1101	26-27	0.6412736906285375
1101	28-29	0.701705011998996
1101	30-31	0.6521864157005766
1101	32-33	0.6684426554292386
1101	34-35	0.5785374385527646
1101	36-37	0.5692792754943738
1101	38-39	0.612360509404688
1101	40-41	0.5567964222010531
1101	42-43	0.552477635323676
1101	44-45	0.4642768439933107
1101	46-47	0.42536974402878514
1101	48-49	0.470393683478207
1101	50-51	0.38716878139512545
1101	52-53	0.3751572034086408
1101	54-55	0.4836951958360629
1101	56-57	0.45436950354269356
1101	58-59	0.4635632430588572
1101	60-61	0.4649767136876477
1101	62-63	0.4218913690349879
1101	64-65	0.43541146836781763
1101	66-67	0.44524159323889023
1101	68-69	0.3508905003273384
1101	70-71	0.3944386107588542
1101	72-73	0.4075152529340329
1101	74-75	0.39317660633883733
1101	76-77	0.39647263794349286
1101	78-79	0.39460819104456846
1101	80-81	0.4238541256338735
1101	82-83	0.4148508997353808
1101	84-85	0.3790717703022146
1101	86-87	0.3774615795993519
1101	88-89	0.4226309100805139
1101	90-91	0.35568238914407857
1101	92-93	0.3763330464865611
1101	94-95	0.428494568778639
1101	96-97	0.46525822986340515
1101	98-99	0.45442218673243673
1101	100	0.41774697759039014
1102	1	0.18010444623513777
1102	2	0.3100809738448689
1102	3	0.21098980979396842
1102	4	0.23678613488239364
1102	5	0.13568485829680554
1102	6	0.2523430934865232
1102	7	0.17739630496157588
1102	8	0.2136657902187835
1102	9	0.2912134036877916
1102	10-11	0.3105873412328677
1102	12-13	0.25629323241319923
1102	14-15	0.3491849582787836
1102	16-17	0.2476079832316671
1102	18-19	0.2419661833857134
1102	20-21	0.24563308392253447
1102	22-23	0.26195031253264744
1102	24-25	0.3419527693372686
1102	26-27	0.3622341302511032
1102	28-29	0.3679793356353471
1102	30-31	0.3277823978927259
1102	32-33	0.35576057496756164
1102	34-35	0.3965399448802387
1102	36-37	0.37752284773898737
1102	38-39	0.33653005225805543
1102	40-41	0.35134576494873926
1102	42-43	0.424210818872659
1102	44-45	0.3210353403858974
1102	46-47	0.2656678776839314
1102	48-49	0.19919504347138428
1102	50-51	0.19845904287947747
1102	52-53	0.2756725345560298
1102	54-55	0.27539999291376915
1102	56-57	0.2333306514906539
1102	58-59	0.26182910191840847
1102	60-61	0.22585004105756923
1102	62-63	0.25261070455788115
1102	64-65	0.22290043378180968
1102	66-67	0.2508352848743485
1102	68-69	0.2131068401873364
1102	70-71	0.19726576757893355
1102	72-73	0.2195240832624492
1102	74-75	0.16605120257999673
1102	76-77	0.13518822957735566
1102	78-79	0.08162208549534
1102	80-81	0.1356486387493021
1102	82-83	0.09109050768561033
1102	84-85	0.09777273908116513
1102	86-87	0.0987428380385964
1102	88-89	0.15963709626887024
1102	90-91	0.14685552085224884
1102	92-93	0.21095356989291858
1102	94-95	0.20740411764825595
1102	96-97	0.19761300732516318
1102	98-99	0.21899598038607593
1102	100	0.22595010927130588
1103	1	0.1613241313138829
1103	2	0.26710936386198725
1103	3	0.28349041876217385
1103	4	0.23541226389331626
1103	5	0.18982379983746966
1103	6	0.29435353063697534
1103	7	0.3278346362435087
1103	8	0.35366583538728946
1103	9	0.4244521369693999
1103	10-11	0.5064385045637891
1103	12-13	0.35111328271155173
1103	14-15	0.3958445765081464
1103	16-17	0.35712850989640543
1103	18-19	0.2839917971530781
1103	20-21	0.33255904145445925
1103	22-23	0.25646248786387105
1103	24-25	0.3288132067296914
1103	26-27	0.30948561072943903
1103	28-29	0.3782769734839704
1103	30-31	0.3571674706704684
1103	32-33	0.45203140530983177
1103	34-35	0.4083841264511605
1103	36-37	0.4016184823645119
1103	38-39	0.4670347102598953
1103	40-41	0.40687041807884583
1103	42-43	0.3555270258153769
1103	44-45	0.3387524919955851
1103	46-47	0.2977400569512696
1103	48-49	0.24314362235737264
1103	50-51	0.23373937312575777
1103	52-53	0.24182722699885062
1103	54-55	0.29285690942140263
1103	56-57	0.27269105903960167
1103	58-59	0.341577403663365
1103	60-61	0.3385279944727131
1103	62-63	0.2875035213141075
1103	64-65	0.31435387554105887
1103	66-67	0.3637499728648521
1103	68-69	0.3132915987213636
1103	70-71	0.3351610315001139
1103	72-73	0.3107062429944065
1103	74-75	0.26343852733024065
1103	76-77	0.2603590681467409
1103	78-79	0.2758195396049494
1103	80-81	0.29154183459831984
1103	82-83	0.3421313609831529
1103	84-85	0.2661004881894904
1103	86-87	0.2822544439240495
1103	88-89	0.2807047764307029
1103	90-91	0.3207561211093868
1103	92-93	0.35212401485106426
1103	94-95	0.3478327086652051
1103	96-97	0.33219260632565195
1103	98-99	0.3286718608895782
1103	100	0.326796947718087
1104	1	0.19080715235750034
1104	2	0.29527730874856317
1104	3	0.27277264194791684
1104	4	0.23112812454325393
1104	5	0.1542527160468552
1104	6	0.3000613443292366
1104	7	0.24495927705982723
1104	8	0.2548973338339948
1104	9	0.3167445066570167
1104	10-11	0.3433498652580198
1104	12-13	0.3355725964667542
1104	14-15	0.3746169383632605
1104	16-17	0.44063965644023995
1104	18-19	0.31478398268378527
1104	20-21	0.3091202584752253
1104	22-23	0.34089164650767145
1104	24-25	0.3334952120352739
1104	26-27	0.4245988788852131
1104	28-29	0.4776682952643654
1104	30-31	0.42452580893796465
1104	32-33	0.4325731964960937
1104	34-35	0.3868951750887888
1104	36-37	0.3778414412143789
1104	38-39	0.3398984294835472
1104	40-41	0.36394690147441366
1104	42-43	0.30882653643976
1104	44-45	0.24093216386992822
1104	46-47	0.2866327564118478
1104	48-49	0.2678460356676382
1104	50-51	0.20270516345418343
1104	52-53	0.15489820278182975
1104	54-55	0.1728652703005764
1104	56-57	0.1791155896725911
1104	58-59	0.1883437854695984
1104	60-61	0.2034647548340729
1104	62-63	0.2927599718567109
1104	64-65	0.3430927037436433
1104	66-67	0.3321941127122017
1104	68-69	0.3613518343489659
1104	70-71	0.25445371977141207
1104	72-73	0.26877211988435334
1104	74-75	0.32055436591243236
1104	76-77	0.26219108907069355
1104	78-79	0.2635804744071848
1104	80-81	0.23430166445908895
1104	82-83	0.24345441882707064
1104	84-85	0.24451384819060706
1104	86-87	0.25629042678580305
1104	88-89	0.21046181674164188
1104	90-91	0.17596358435959303
1104	92-93	0.18023397439870692
1104	94-95	0.21472696530068092
1104	96-97	0.24811503974554583
1104	98-99	0.2585355474778268
1104	100	0.20384650846111896
1105	1	0.07860879981118885
1105	2	0.18450169352384904
1105	3	0.1408910535740837
1105	4	0.0850072930347281
1105	5	0.06187591801767667
1105	6	0.10142754921653818
1105	7	0.1792382799904857
1105	8	0.07850218479070037
1105	9	0.1574570845882448
1105	10-11	0.25627083593804656
1105	12-13	0.1519798988215868
1105	14-15	0.2534848130642615
1105	16-17	0.24512400872524864
1105	18-19	0.208211217015716
1105	20-21	0.18575500140871526
1105	22-23	0.17015026192464688
1105	24-25	0.18815077621957954
1105	26-27	0.25583883646684313
1105	28-29	0.3160639812448167
1105	30-31	0.34207437103925287
1105	32-33	0.35344938430844763
1105	34-35	0.3564449228404456
1105	36-37	0.2766646316003545
1105	38-39	0.21887258247800645
1105	40-41	0.2085664972896737
1105	42-43	0.25239196010194576
1105	44-45	0.18311462222376207
1105	46-47	0.15991897850490489
1105	48-49	0.14240769310404033
1105	50-51	0.13024511884628254
1105	52-53	0.14325475875473614
1105	54-55	0.23714403032310827
1105	56-57	0.2505298058059111
1105	58-59	0.2148342639741685
1105	60-61	0.15742411893988617
1105	62-63	0.18500909579442748
1105	64-65	0.18876117037388696
1105	66-67	0.17632625731929608
1105	68-69	0.17797091473904914
1105	70-71	0.21612412923546032
1105	72-73	0.2252851694406246
1105	74-75	0.21875782915945408
1105	76-77	0.14280334474860013
1105	78-79	0.2010573005169256
1105	80-81	0.20620581238601687
1105	82-83	0.1660949724391223
1105	84-85	0.13264996743578905
1105	86-87	0.14723680618270762
1105	88-89	0.18273633967032055
1105	90-91	0.16693683456947994
1105	92-93	0.13961507060810163
1105	94-95	0.17278600289869317
1105	96-97	0.2718089426414174
1105	98-99	0.27188451676403247
1105	100	0.2612637607543604
1106	1	0.15152869309141792
1106	2	0.18162488857224446
1106	3	0.13830735041111097
1106	4	0.18803503649240838
1106	5	0.09485028294437825
1106	6	0.20464530598039232
1106	7	0.19291627799957212
1106	8	0.21112227774514025
1106	9	0.3145631044381929
1106	10-11	0.35786218135345393
1106	12-13	0.26596127134112635
1106	14-15	0.3433258311859717
1106	16-17	0.29511863637724645
1106	18-19	0.3115215273323315
1106	20-21	0.324103619713064
1106	22-23	0.33058558176814046
1106	24-25	0.30643482263013766
1106	26-27	0.3589422647707039
1106	28-29	0.44888875366586234
1106	30-31	0.34746006250704653
1106	32-33	0.36191829076642534
1106	34-35	0.30369715329715774
1106	36-37	0.2933304417212952
1106	38-39	0.326323726158833
1106	40-41	0.37638026513757694
1106	42-43	0.34283959905405936
1106	44-45	0.29067510658813234
1106	46-47	0.24955147727136762
1106	48-49	0.2598510078404317
1106	50-51	0.2565030815167404
1106	52-53	0.2652471237275833
1106	54-55	0.2962379471237284
1106	56-57	0.22579873785538496
1106	58-59	0.30444513933126416
1106	60-61	0.24306975280585874
1106	62-63	0.3295754785540552
1106	64-65	0.3565760977632806
1106	66-67	0.3529621023243692
1106	68-69	0.35785068485382965
1106	70-71	0.3208143696719148
1106	72-73	0.2561057122738575
1106	74-75	0.34782082757291377
1106	76-77	0.38326514875275564
1106	78-79	0.3499895667457835
1106	80-81	0.274288850240211
1106	82-83	0.2961873814862024
1106	84-85	0.32696007404601346
1106	86-87	0.35595093527149047
1106	88-89	0.3442540419648594
1106	90-91	0.2790696998442783
1106	92-93	0.24928065301607205
1106	94-95	0.3202219944604927
1106	96-97	0.42053456575803594
1106	98-99	0.4419026487298794
1106	100	0.41236476579131676
1107	1	0.10303472326512164
1107	2	0.0729078193805286
1107	3	0.12471358568405222
1107	4	0.1832896952367804
1107	5	0.06601328302560461
1107	6	0.22772151106774174
1107	7	0.21569281631102655
1107	8	0.21400762502405257
1107	9	0.3000530434411317
1107	10-11	0.24904214814822012
1107	12-13	0.1724144925903559
1107	14-15	0.25832425963298533
1107	16-17	0.21884114155902523
1107	18-19	0.2504206674679281
1107	20-21	0.2251057440960338
1107	22-23	0.2617174262900832
1107	24-25	0.30075863845110007
1107	26-27	0.2930688736977274
1107	28-29	0.36003764034960994
1107	30-31	0.2404122975274703
1107	32-33	0.25403373598004464
1107	34-35	0.2829022521363811
1107	36-37	0.22321004515838183
1107	38-39	0.29053376314391244
1107	40-41	0.21612295684460747
1107	42-43	0.18585959175729982
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2216	100	-1.6498181100119744
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	207888.0
3	42465.0
4	27271.0
5	22718.0
6	16085.0
7	14584.0
8	15896.0
9	17146.0
10	17701.0
11	18844.0
12	20190.0
13	21663.0
14	22839.0
15	23609.0
16	23648.0
17	23443.0
18	23102.0
19	22022.0
20	22058.0
21	23764.0
22	28449.0
23	41965.0
24	63076.0
25	81654.0
26	89688.0
27	76146.0
28	77033.0
29	93190.0
30	108074.0
31	123750.0
32	154546.0
33	201096.0
34	275311.0
35	410448.0
36	665555.0
37	1110513.0
38	1404440.0
39	590577.0
40	1973.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.631247746482586	21.61744224086237	14.868940890198026	21.88236912245702
2	15.16156138786462	21.909767912659966	39.68799556551708	23.240675133958337
3	17.57590695336807	28.185206346472352	29.807092254190525	24.431794445969057
4	11.889998828701348	19.138489757403	38.60746568729604	30.364045726599613
5	12.35294268870092	40.511658218402886	34.062506226135824	13.072892866760371
6	28.34487902491099	39.89309858254234	18.382650901926482	13.379371490620176
7	25.848676230734984	35.20861354215388	22.127786951372485	16.814923275738654
8	24.56849583365869	37.59125670838632	20.072250216342546	17.76799724161245
9	25.199389972724866	19.19045743048183	21.119671901470184	34.49048069532312
10-11	22.9192527523235	29.659958991094815	27.90392984334547	19.516858413236218
12-13	23.397202644511303	27.63473661691532	27.87396435933214	21.094096379241243
14-15	21.376295615411145	29.22363534454828	25.498188482202828	23.901880557837742
16-17	20.515671309547546	32.2341081358035	26.34439526092286	20.905825293726092
18-19	20.5970457638846	31.096920804965194	28.087214336720383	20.218819094429822
20-21	22.537444652222597	29.684274860769982	27.416885749526877	20.361394737480545
22-23	22.02771260026465	29.726231951398674	27.590211676425618	20.65584377191105
24-25	21.355925967342237	29.990943216522197	27.601981599160936	21.051149216974633
26-27	21.1428251010662	30.090900334502756	27.66319069468749	21.103083869743557
28-29	21.047918049383405	30.04894505926939	27.97457469634998	20.928562194997223
30-31	21.392834551963887	30.278348724940606	27.512406285090623	20.816410438004883
32-33	21.003801613044587	30.0374345173097	27.921246368030815	21.0375175016149
34-35	21.409929578471782	29.713929311123504	27.834425942679825	21.041715167724895
36-37	21.431979327075982	29.969489811714727	27.511957167644884	21.086573693564407
38-39	21.265554490956966	29.97997363933576	27.72815080992727	21.026321059780003
40-41	21.242298126132752	29.663867767203477	27.928182172598614	21.165651934065156
42-43	21.14063325420012	29.880806940243932	27.923199869410926	21.055359936145027
44-45	21.308565393525818	29.792541806903994	27.723567092962437	21.17532570660775
46-47	21.238221232364886	29.79443179541444	27.756206321415895	21.211140650804776
48-49	21.127883059341528	29.68699905569928	27.753749187142247	21.43136869781694
50-51	21.178267761369657	29.684403695666912	27.66288398391171	21.47444455905172
52-53	21.260035694429526	29.71432326345122	27.663383712064828	21.362257330054423
54-55	21.326327051249024	29.86712115599798	27.579941513096973	21.22661027965602
56-57	21.229666317867775	29.69630557546435	27.598603968981163	21.475424137686716
58-59	21.438709236892947	29.347372564455814	27.782286148074657	21.431632050576578
60-61	21.334749741854083	29.81524673158432	27.547225800999424	21.302777725562173
62-63	21.148975597561396	29.745683551368895	27.74902983734171	21.356311013728
64-65	21.40273991882555	29.542669926669223	27.652862425985592	21.401727728519635
66-67	21.080945081871143	29.52636575149439	27.713466538639537	21.679222627994932
68-69	21.408499333364983	29.757852873728314	27.719267973137946	21.114379819768754
70-71	21.44392997329888	29.539315269413986	27.663882374319236	21.3528723829679
72-73	21.35396353672335	29.517356225672103	27.834997467549083	21.293682770055465
74-75	21.421250973210874	29.57876637829956	27.586596086720277	21.413386561769286
76-77	21.409714823382586	29.4992288604993	27.40702645893336	21.684029857184754
78-79	21.492012783659124	29.47466628621592	27.690781267170895	21.342539662954056
80-81	21.23948722673899	29.69146251109287	27.681791960659076	21.387258301509064
82-83	21.271097737016422	29.678164871419376	27.84778380879472	21.20295358276948
84-85	21.28718458676572	29.30974414448168	27.744660368329573	21.658410900423032
86-87	21.589213647202136	29.501799859597217	27.510350467941002	21.39863602525965
88-89	21.350552933441136	29.50921810114467	27.623818090460738	21.516410874953458
90-91	21.27377527123569	29.40721891512795	27.582885347308363	21.736120466328007
92-93	21.318244406901353	29.523330139644816	27.62609921781007	21.532326235643765
94-95	21.372023247687483	29.310072257411505	27.76634226476176	21.551562230139254
96-97	21.437890845154147	29.515994648100392	27.500509291599485	21.54560521514598
98-99	21.20235011411335	29.509180439514942	27.615455472978578	21.673013973393125
100	21.30882290093849	29.45522292381681	27.54449784503603	21.691456330208663
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29689.0
1	22611.0
2	13428.0
3	9541.5
4	6666.0
5	4905.5
6	4022.0
7	3823.5
8	3900.0
9	3882.0
10	3424.0
11	2954.5
12	2820.5
13	2858.0
14	2974.5
15	3191.5
16	3593.5
17	4181.0
18	5143.0
19	6556.0
20	8757.5
21	11778.0
22	16342.0
23	23906.0
24	32853.0
25	42508.0
26	55370.0
27	71434.0
28	90359.5
29	108607.5
30	122647.0
31	136893.0
32	150773.5
33	162636.0
34	173665.0
35	184467.0
36	192629.0
37	197701.5
38	202192.0
39	204124.5
40	206748.0
41	206844.0
42	203006.5
43	204096.0
44	203781.0
45	198868.5
46	196762.5
47	194050.0
48	191639.0
49	191543.0
50	190148.5
51	182601.0
52	171684.5
53	161335.0
54	155119.0
55	152792.5
56	138518.5
57	118649.0
58	106592.5
59	97996.0
60	85363.0
61	70759.0
62	59122.5
63	48299.0
64	38422.0
65	30089.0
66	23717.5
67	18769.5
68	14434.0
69	10985.0
70	8482.5
71	6519.0
72	4914.0
73	3857.0
74	3227.5
75	2495.0
76	1723.5
77	1318.5
78	1036.0
79	779.0
80	581.5
81	363.0
82	235.5
83	181.5
84	162.5
85	138.0
86	109.5
87	87.0
88	69.5
89	61.0
90	47.5
91	39.5
92	37.5
93	28.0
94	18.0
95	18.5
96	20.5
97	25.5
98	33.5
99	32.0
100	47.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01310965519678942
2	0.007550904341288024
3	0.0098965044132626
4	0.008980756439957457
5	0.010539134569967966
6	0.011920789406884498
7	0.010266016753368185
8	0.007936482435311242
9	0.007004668708088465
10-11	0.0065226960905594425
12-13	0.006980570077212013
14-15	0.007960581066187694
16-17	0.005446290578077957
18-19	0.006016624842153968
20-21	0.005896131687771712
22-23	0.006297775535712565
24-25	0.005719408394677737
26-27	0.0065146632136006246
28-29	0.016282641595522153
30-31	0.005735474148595371
32-33	0.005647112502048384
34-35	0.005285633038901616
36-37	0.00696450432329438
38-39	0.006000559088236333
40-41	0.005888098810812895
42-43	0.005076778237972373
44-45	0.0047956275444137764
46-47	0.00647449882880654
48-49	0.0038156165554380974
50-51	0.004273490542090669
52-53	0.0032051179065680016
54-55	0.004683167266990338
56-57	0.0032613480452797212
58-59	0.003132822013938648
60-61	0.0036469261393029394
62-63	0.003309545307032623
64-65	0.004723331651784423
66-67	0.0035103672310030495
68-69	0.004008405602449706
70-71	0.005414159070242689
72-73	0.0034139727074972447
74-75	0.0028597041973388686
76-77	0.0030444603673916606
78-79	0.0035987288775500364
80-81	0.004265457665131852
82-83	0.0018475617005279208
84-85	0.0029641315978034904
86-87	0.0029641315978034904
88-89	0.001887726085322006
90-91	0.0028034740586271494
92-93	0.002642816519450808
94-95	0.0023295343180569435
96-97	0.0014137863447518002
98-99	0.002032317870580713
100	0.002988230228679941
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6224420.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.749216997022096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.12274612488791	36.80546422640937
2	15.370569070215565	15.90829828364603
3	5.844246327139956	9.07305514101645
4	2.825064169785421	5.847794350109514
5	1.4908124750078378	3.8574189135524093
6	0.8890099964712206	2.7603342731946627
7	0.5658897388265255	2.049904562364542
8	0.39446956866988914	1.6330793046255843
9	0.2522787235959096	1.1749703769986837
>10	1.114935528387199	9.867015108122128
>50	0.07227124709012436	2.6049867235871615
>100	0.05199353779392578	5.119304480674428
>500	0.0039963515352856	1.366446109683986
>1k	0.00168591985531441	1.3607478448672077
>5k	0.0	0.0
>10k+	3.122073800692683E-5	0.5711803011477956
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35353	0.5679725982501181	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.213150783526818E-5	0.0
5	0.0	0.0	0.0	6.426301567053637E-5	0.0
6	0.0	0.0	0.0	8.032876958817046E-5	0.0
7	0.0	0.0	0.0	9.639452350580456E-5	0.0
8	0.0	0.0	0.0	9.639452350580456E-5	0.0
9	0.0	0.0	0.0	1.927890470116091E-4	0.0
10-11	0.0	0.0	0.0	2.3295343180569433E-4	0.0
12-13	0.0	1.606575391763409E-5	0.0	2.5705206268214546E-4	0.0
14-15	0.0	1.606575391763409E-5	0.0	2.891835705174137E-4	0.0
16-17	0.0	1.606575391763409E-5	0.0	3.052493244350478E-4	0.0
18-19	0.0	1.606575391763409E-5	0.0	3.614794631467671E-4	0.0
20-21	0.0	1.606575391763409E-5	0.0	4.498411096937546E-4	0.0
22-23	0.0	1.606575391763409E-5	0.0	6.747616645406319E-4	0.0
24-25	0.0	1.606575391763409E-5	0.0	0.0011567342820696547	0.0
26-27	0.0	1.606575391763409E-5	0.0	0.0016708384074339455	0.0
28-29	0.0	1.606575391763409E-5	0.0	0.0031569206448150994	0.0
30-31	0.0	1.606575391763409E-5	0.0	0.0066913865066946	0.0
32-33	0.0	1.606575391763409E-5	0.0	0.013455068906018552	0.0
34-35	0.0	1.606575391763409E-5	0.0	0.023833545936810176	0.0
36-37	0.0	1.606575391763409E-5	0.0	0.04044553548764383	0.0
38-39	0.0	1.606575391763409E-5	0.0	0.0655000787221942	0.0
40-41	0.0	1.606575391763409E-5	0.0	0.09358301657021859	0.0
42-43	0.0	1.606575391763409E-5	0.0	0.12222825580536018	0.0
44-45	0.0	1.606575391763409E-5	0.0	0.15648044315775606	0.0
46-47	0.0	1.606575391763409E-5	0.0	0.199247480086498	0.0
48-49	0.0	1.606575391763409E-5	0.0	0.24855327885971706	0.0
50-51	0.0	1.606575391763409E-5	0.0	0.30006811879661077	0.0
52-53	0.0	1.606575391763409E-5	0.0	0.35063507925236403	0.0
54-55	0.0	1.606575391763409E-5	0.0	0.4065599686396484	0.0
56-57	0.0	1.606575391763409E-5	0.0	0.4737871159079882	0.0
58-59	0.0	1.606575391763409E-5	0.0	0.5508143730660848	0.0
60-61	0.0	1.606575391763409E-5	0.0	0.6359227044447515	0.0
62-63	0.0	1.606575391763409E-5	0.0	0.7232802413718868	0.0
64-65	0.0	1.606575391763409E-5	0.0	0.812838786585738	0.0
66-67	0.0	1.606575391763409E-5	0.0	0.9172661870503598	0.0
68-69	0.0	1.606575391763409E-5	0.0	1.0381529524035975	0.0
70-71	0.0	1.606575391763409E-5	0.0	1.1719726496605305	0.0
72-73	0.0	1.606575391763409E-5	0.0	1.314267032109016	0.0
74-75	0.0	1.606575391763409E-5	0.0	1.4569791241593595	0.0
76-77	0.0	1.606575391763409E-5	0.0	1.6116521700013817	0.0
78-79	0.0	1.606575391763409E-5	0.0	1.7931791235167291	0.0
80-81	0.0	1.606575391763409E-5	0.0	1.9944348228429316	0.0
82-83	0.0	1.606575391763409E-5	0.0	2.2066313005870426	0.0
84-85	0.0	1.606575391763409E-5	0.0	2.422161422269063	0.0
86-87	0.0	1.606575391763409E-5	0.0	2.647877231934863	0.0
88	0.0	1.606575391763409E-5	0.0	2.8335330842070428	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8935	0.0	47.139732	1
GTATCAA	15960	0.0	38.260437	1
ATCAACG	18970	0.0	31.023424	3
TCAACGC	19035	0.0	30.892792	4
TATCAAC	20535	0.0	29.09471	2
CAACGCA	21505	0.0	27.365955	5
GTACATG	35380	0.0	26.001995	1
AACGCAG	22785	0.0	25.764858	6
TACATGG	35140	0.0	24.975016	2
CATGGGT	15865	0.0	24.473309	4
CGCAGAG	23700	0.0	24.035774	8
ACGCAGA	24055	0.0	23.89637	7
ACATGGG	36525	0.0	23.30671	3
GCAGAGT	25310	0.0	21.374062	9
ATGGGTG	9710	0.0	20.81589	5
GTGGTAT	4910	0.0	20.296822	1
TGGTATC	4660	0.0	19.771214	2
ATGGGTA	6780	0.0	18.44156	5
AGAGTAC	23770	0.0	18.250202	10-11
CAGAGTA	25130	0.0	17.7862	10-11
>>END_MODULE