FastQCFastQC Report
Wed 25 May 2016
SRR2431447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6224420
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT547640.8798249475453135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436310.7009649091802931No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352180.5658037214712375No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98210.15778176922508444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98050.1575247171624023No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79060.12701585047281513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA181100.062.2680931
GTATCAA357000.045.5561641
GTGGTAT60450.037.1225741
TGGTATC61600.035.2692072
ATCAACG493350.032.8183173
TATCAAC496900.032.669032
TCAACGC500900.032.333044
CAACGCA525650.030.8173985
AACGCAG543850.029.8710866
ACGCAGA604550.026.6463837
CGCAGAG604550.026.5764058
GCAGAGT634100.025.0487989
GTACATG337050.024.4264131
CATGGGT147700.024.1018184
TACATGG336800.023.9875282
ACATGGG346050.022.6261043
AGTACTT451500.022.41052212-13
AGAGTAC621450.022.3591210-11
GAGTACT443200.022.1091512-13
CAGAGTA631200.021.12008710-11
Produced by FastQC (version 0.11.5)