##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431447_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6224420
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25542942153646	33.0	31.0	34.0	30.0	34.0
2	32.38590021238927	34.0	31.0	34.0	31.0	34.0
3	32.4851375710508	34.0	31.0	34.0	31.0	34.0
4	36.03649480594176	37.0	35.0	37.0	35.0	37.0
5	35.94995903232751	37.0	35.0	37.0	35.0	37.0
6	35.99085810404825	37.0	35.0	37.0	35.0	37.0
7	35.92572785898124	37.0	35.0	37.0	35.0	37.0
8	35.9106856542457	37.0	35.0	37.0	35.0	37.0
9	37.59205966178375	39.0	37.0	39.0	35.0	39.0
10-11	37.52500498038371	39.0	37.0	39.0	34.5	39.0
12-13	37.502615826052875	39.0	37.0	39.0	34.0	39.0
14-15	38.80352097062858	40.0	38.0	41.0	34.0	41.0
16-17	38.87364019458841	40.0	38.0	41.0	35.0	41.0
18-19	38.97933550435221	40.0	38.5	41.0	35.0	41.0
20-21	39.01706102737283	41.0	39.0	41.0	35.0	41.0
22-23	38.958373711928175	40.0	38.5	41.0	35.0	41.0
24-25	38.90570527053123	40.5	38.0	41.0	35.0	41.0
26-27	38.765166874985944	40.0	38.0	41.0	34.5	41.0
28-29	38.66776366312042	40.0	38.0	41.0	34.0	41.0
30-31	38.459013691235484	40.0	38.0	41.0	34.0	41.0
32-33	38.23206258575097	40.0	37.5	41.0	34.0	41.0
34-35	38.077388977607555	40.0	37.0	41.0	33.5	41.0
36-37	37.923202563451696	40.0	37.0	41.0	33.0	41.0
38-39	37.80632227581044	40.0	37.0	41.0	33.0	41.0
40-41	37.664472191786544	40.0	37.0	41.0	33.0	41.0
42-43	37.50850681669939	40.0	37.0	41.0	33.0	41.0
44-45	37.242801256984585	40.0	37.0	41.0	32.0	41.0
46-47	36.85314551395953	40.0	36.0	41.0	31.0	41.0
48-49	36.54914803306975	40.0	36.0	41.0	30.0	41.0
50-51	36.39341376385269	40.0	36.0	41.0	30.0	41.0
52-53	36.248261685426115	40.0	35.5	41.0	30.0	41.0
54-55	36.09326041623155	40.0	35.0	41.0	29.5	41.0
56-57	35.86643542691528	39.0	35.0	41.0	29.0	41.0
58-59	35.69333110554879	39.0	35.0	41.0	28.0	41.0
60-61	35.490088072462974	39.0	35.0	41.0	28.0	41.0
62-63	35.272096275636926	38.5	35.0	41.0	28.0	41.0
64-65	35.033321176912864	38.0	35.0	40.5	28.0	41.0
66-67	34.77728189935769	37.0	35.0	40.0	28.0	41.0
68-69	34.49789345834632	37.0	34.5	40.0	27.0	41.0
70-71	34.17899298247869	36.0	34.0	39.5	26.0	41.0
72-73	33.87104653927595	36.0	34.0	39.0	26.0	41.0
74-75	33.56893188441654	35.5	34.0	39.0	26.0	41.0
76-77	33.226211277516626	35.0	34.0	37.5	26.0	40.0
78-79	32.921831592341135	35.0	34.0	37.0	26.0	39.0
80-81	32.625582303250745	35.0	33.5	37.0	25.0	39.0
82-83	32.376177700090935	35.0	33.0	36.0	25.0	39.0
84-85	32.154309477830864	35.0	33.0	36.0	25.0	37.0
86-87	31.916968006657648	35.0	33.0	36.0	25.0	37.0
88-89	31.769021049350783	35.0	33.0	35.0	25.0	37.0
90-91	31.583295873350448	35.0	33.0	35.0	24.0	36.0
92-93	31.391808152406167	35.0	33.0	35.0	24.0	36.0
94-95	31.233094248138784	35.0	33.0	35.0	23.0	36.0
96-97	31.070859694557885	35.0	33.0	35.0	20.0	36.0
98-99	30.90947373409892	35.0	33.0	35.0	19.5	35.5
100	30.799731541252036	35.0	33.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1646078580970851
1101	2	0.2286682342455606
1101	3	0.20322642262784996
1101	4	0.19534070790687963
1101	5	0.17979331943514154
1101	6	0.17767625663454822
1101	7	0.21523601170944318
1101	8	0.24159683668285226
1101	9	0.3246943372617537
1101	10-11	0.3054096327802185
1101	12-13	0.3406139275405309
1101	14-15	0.39085478140539465
1101	16-17	0.38735312081040973
1101	18-19	0.3508584574331479
1101	20-21	0.3327866006999116
1101	22-23	0.33618439898062036
1101	24-25	0.34968732669668867
1101	26-27	0.36469024183252685
1101	28-29	0.4204906770927863
1101	30-31	0.4144784161881816
1101	32-33	0.3878783761019875
1101	34-35	0.43184797162349753
1101	36-37	0.49140733553822
1101	38-39	0.47704613110506244
1101	40-41	0.5105993025252431
1101	42-43	0.5482550927607832
1101	44-45	0.5994126854876143
1101	46-47	0.5876270472866736
1101	48-49	0.5310528988285199
1101	50-51	0.47165528650211996
1101	52-53	0.47323456457537105
1101	54-55	0.506087899717528
1101	56-57	0.506911559498981
1101	58-59	0.5174381949121809
1101	60-61	0.5373528899146862
1101	62-63	0.5559470159192514
1101	64-65	0.5106946374667629
1101	66-67	0.5106038007075853
1101	68-69	0.4591503380316695
1101	70-71	0.4624952512554188
1101	72-73	0.46084979623912403
1101	74-75	0.48532168232353
1101	76-77	0.4616264213379395
1101	78-79	0.47500792810455295
1101	80-81	0.47529733720737966
1101	82-83	0.45663592605814785
1101	84-85	0.4377102561682449
1101	86-87	0.5024621769917488
1101	88-89	0.48586952768835445
1101	90-91	0.5237322957300208
1101	92-93	0.535386514668275
1101	94-95	0.5798989723560766
1101	96-97	0.559290977362533
1101	98-99	0.6546609837706576
1101	100	0.6619855699062391
1102	1	0.1167690477719674
1102	2	0.21264937344603396
1102	3	0.2432871623400743
1102	4	0.15669327471876926
1102	5	0.1143652535996722
1102	6	0.12641500149506157
1102	7	0.14620850529276908
1102	8	0.19291221753072563
1102	9	0.2660966648607612
1102	10-11	0.23420052154425264
1102	12-13	0.27358234035959583
1102	14-15	0.37165804146650316
1102	16-17	0.36190675027101804
1102	18-19	0.3408580912758694
1102	20-21	0.30445016170069295
1102	22-23	0.3106756395071244
1102	24-25	0.32181575339259894
1102	26-27	0.3326876876583995
1102	28-29	0.2929926065941757
1102	30-31	0.2468286986447552
1102	32-33	0.2728632273156748
1102	34-35	0.3056185814494228
1102	36-37	0.2737849441767608
1102	38-39	0.28074172977478185
1102	40-41	0.30547893072327525
1102	42-43	0.3284353956611952
1102	44-45	0.2963191331196384
1102	46-47	0.2923803796635127
1102	48-49	0.2913673459029198
1102	50-51	0.30273750745443095
1102	52-53	0.31656552326268184
1102	54-55	0.3093381518637486
1102	56-57	0.32909352534065306
1102	58-59	0.2843778569986526
1102	60-61	0.26447854383491887
1102	62-63	0.25276462992493975
1102	64-65	0.32430132759338903
1102	66-67	0.3239943777257679
1102	68-69	0.30894817273737374
1102	70-71	0.2963336779909653
1102	72-73	0.21335596242114718
1102	74-75	0.2677766632468135
1102	76-77	0.2813909196684321
1102	78-79	0.23881898626234488
1102	80-81	0.2367534813679697
1102	82-83	0.2353704738688549
1102	84-85	0.2499721049933754
1102	86-87	0.25315918916548696
1102	88-89	0.22141359954871476
1102	90-91	0.20094539356422558
1102	92-93	0.2107832235634568
1102	94-95	0.23197091629664612
1102	96-97	0.23825236098885938
1102	98-99	0.30944794244659235
1102	100	0.273393253197284
1103	1	0.16615787598808396
1103	2	0.22368415480628556
1103	3	0.17119676080821478
1103	4	0.1800315174381666
1103	5	0.11034110135600628
1103	6	0.15845472396687654
1103	7	0.16197005431430966
1103	8	0.17569714609189901
1103	9	0.2607067848637925
1103	10-11	0.25825502702174674
1103	12-13	0.22051360841691547
1103	14-15	0.31938018419575087
1103	16-17	0.3281759448682209
1103	18-19	0.3062418283564696
1103	20-21	0.30211280138025387
1103	22-23	0.2734302148344696
1103	24-25	0.33397950857285963
1103	26-27	0.3041518020895708
1103	28-29	0.3102347791346247
1103	30-31	0.35759128815501384
1103	32-33	0.35986880401259214
1103	34-35	0.37620307800613517
1103	36-37	0.37415820377788833
1103	38-39	0.3057914264198942
1103	40-41	0.3598970136106985
1103	42-43	0.4075394009781732
1103	44-45	0.4211925241933727
1103	46-47	0.49487858502211424
1103	48-49	0.4138900713058504
1103	50-51	0.37001965734610565
1103	52-53	0.42122603518312474
1103	54-55	0.4269869119076972
1103	56-57	0.42534413309898866
1103	58-59	0.4537284533035404
1103	60-61	0.45571204079652716
1103	62-63	0.43149971826969136
1103	64-65	0.43571436290063303
1103	66-67	0.43253869689930724
1103	68-69	0.43382266721310714
1103	70-71	0.47129391841644974
1103	72-73	0.4362269627905633
1103	74-75	0.45161795403356564
1103	76-77	0.43661245988211306
1103	78-79	0.4242010456557068
1103	80-81	0.4200302691131057
1103	82-83	0.3889145010602917
1103	84-85	0.37703083211270183
1103	86-87	0.36095185283274844
1103	88-89	0.32865196180333456
1103	90-91	0.35473078187622775
1103	92-93	0.3723597720768481
1103	94-95	0.40618863815868167
1103	96-97	0.40290394890369896
1103	98-99	0.45665502873940156
1103	100	0.4445323479882255
1104	1	0.12735557659090802
1104	2	0.1621433349117396
1104	3	0.1415622244718051
1104	4	0.13415515588143023
1104	5	0.10880451473931885
1104	6	0.111333107364473
1104	7	0.12991929227767685
1104	8	0.11651474061412159
1104	9	0.23102941844748415
1104	10-11	0.25840347769488403
1104	12-13	0.24533637487331816
1104	14-15	0.3230348954268294
1104	16-17	0.254378722607143
1104	18-19	0.2748902223061762
1104	20-21	0.20156769070738534
1104	22-23	0.23891741942580325
1104	24-25	0.2464164686317858
1104	26-27	0.2658032653211322
1104	28-29	0.2741002093857787
1104	30-31	0.27833804831433184
1104	32-33	0.22576385788431708
1104	34-35	0.29288458601035927
1104	36-37	0.2366619420854832
1104	38-39	0.2457972448458392
1104	40-41	0.28278507262794506
1104	42-43	0.30864947708082724
1104	44-45	0.32194947903063564
1104	46-47	0.35794071816523143
1104	48-49	0.30881405413030194
1104	50-51	0.2615741499200013
1104	52-53	0.2415874365162125
1104	54-55	0.2838683474924082
1104	56-57	0.3121062754110966
1104	58-59	0.33823659583633514
1104	60-61	0.3059532661470854
1104	62-63	0.30000565402767876
1104	64-65	0.287853153494801
1104	66-67	0.30202764826168504
1104	68-69	0.27892890787899205
1104	70-71	0.3044024653174091
1104	72-73	0.2927687477605616
1104	74-75	0.2502300170105656
1104	76-77	0.2694730088095696
1104	78-79	0.2540811618377319
1104	80-81	0.23846760525619004
1104	82-83	0.2396748157898969
1104	84-85	0.24269475895967219
1104	86-87	0.2310708166432036
1104	88-89	0.19860553561295902
1104	90-91	0.22086662906795596
1104	92-93	0.21996072503058528
1104	94-95	0.19786284981946878
1104	96-97	0.1876435852210001
1104	98-99	0.19474138201296753
1104	100	0.2638040066281384
1105	1	0.10573704094744585
1105	2	0.14818718514637652
1105	3	0.15108035416164967
1105	4	0.10804977898312274
1105	5	0.11551064750830164
1105	6	0.1398814117531728
1105	7	0.12600318929440135
1105	8	0.15180039170540027
1105	9	0.2063936476820416
1105	10-11	0.20109317444733676
1105	12-13	0.14837594963749012
1105	14-15	0.2740056451682733
1105	16-17	0.24823814175775993
1105	18-19	0.24981101245803927
1105	20-21	0.2149138691852599
1105	22-23	0.21869901914972445
1105	24-25	0.20636251787487225
1105	26-27	0.24438338612264232
1105	28-29	0.24207447995352283
1105	30-31	0.2027027166041293
1105	32-33	0.24824665776122146
1105	34-35	0.22198475897339165
1105	36-37	0.23805286658758007
1105	38-39	0.19087217840903037
1105	40-41	0.15864749828164548
1105	42-43	0.23551056535043102
1105	44-45	0.2356143438652083
1105	46-47	0.21233120346835221
1105	48-49	0.19912745304308288
1105	50-51	0.16075564775665896
1105	52-53	0.1851028951269882
1105	54-55	0.17458852768136524
1105	56-57	0.20737186789683193
1105	58-59	0.24194680903060828
1105	60-61	0.22194901889612595
1105	62-63	0.18255220339320033
1105	64-65	0.17336732627358487
1105	66-67	0.17767981106148056
1105	68-69	0.19714171829772908
1105	70-71	0.25306500695375433
1105	72-73	0.2424114679981031
1105	74-75	0.18557748630317406
1105	76-77	0.21180162526957247
1105	78-79	0.21984489660333395
1105	80-81	0.15585107846771962
1105	82-83	0.15596789742633632
1105	84-85	0.15575383273039023
1105	86-87	0.1444972590084106
1105	88-89	0.16984526753437734
1105	90-91	0.13861809970417127
1105	92-93	0.17702373151738016
1105	94-95	0.21309545902308002
1105	96-97	0.19683261512595962
1105	98-99	0.1896722200330636
1105	100	0.18659629397417277
1106	1	0.06677075994777226
1106	2	0.14436142076380776
1106	3	0.12025043011336578
1106	4	0.1599094970711974
1106	5	0.11346379457381772
1106	6	0.1180875439679383
1106	7	0.10723023590843894
1106	8	0.13665758397415573
1106	9	0.15892800914992478
1106	10-11	0.14395070187602244
1106	12-13	0.14188970179954197
1106	14-15	0.19400242758631236
1106	16-17	0.21462729926405189
1106	18-19	0.2040893709388527
1106	20-21	0.20009149695682282
1106	22-23	0.19012408281962223
1106	24-25	0.19749472331812257
1106	26-27	0.18793497779436308
1106	28-29	0.16967759815286598
1106	30-31	0.17016210759661732
1106	32-33	0.21828123890104223
1106	34-35	0.20659627994454866
1106	36-37	0.2055188593916597
1106	38-39	0.13269838400004375
1106	40-41	0.1381688302323809
1106	42-43	0.11725872389366288
1106	44-45	0.16712994150454108
1106	46-47	0.1677398643459327
1106	48-49	0.18689075927694176
1106	50-51	0.23734967934606033
1106	52-53	0.1586328529010359
1106	54-55	0.14805521760877838
1106	56-57	0.2136546424244017
1106	58-59	0.21620536091129594
1106	60-61	0.22804329509634869
1106	62-63	0.20029546645715612
1106	64-65	0.2425169907989968
1106	66-67	0.2684784880887463
1106	68-69	0.30947338958274884
1106	70-71	0.2833550042011481
1106	72-73	0.2523316251177192
1106	74-75	0.24866875049240633
1106	76-77	0.24834180751919632
1106	78-79	0.220908219455346
1106	80-81	0.24805812060206733
1106	82-83	0.21322779251055124
1106	84-85	0.21123633134534714
1106	86-87	0.24892868861934403
1106	88-89	0.19729511649098797
1106	90-91	0.2575101245992357
1106	92-93	0.2820029192043805
1106	94-95	0.2746249781524881
1106	96-97	0.30663160307348747
1106	98-99	0.33594815734463523
1106	100	0.3812404592429779
1107	1	0.08529318234223382
1107	2	0.1559936450134174
1107	3	0.07653950281022048
1107	4	0.0884628744011593
1107	5	0.0876882107400263
1107	6	0.12440411812760743
1107	7	0.028335063665487326
1107	8	0.022180428707407884
1107	9	0.11987568046506425
1107	10-11	0.09254987630056632
1107	12-13	0.0427924459613962
1107	14-15	0.13445283541560116
1107	16-17	0.10431216159355472
1107	18-19	0.08695308171412108
1107	20-21	0.09078438235154351
1107	22-23	0.07954190403044947
1107	24-25	0.09268620557523377
1107	26-27	0.13766870544774434
1107	28-29	0.15066959760780918
1107	30-31	0.17537721144686458
1107	32-33	0.13606644604624307
1107	34-35	0.0990649430522268
1107	36-37	0.11713848606795096
1107	38-39	0.06393737076876249
1107	40-41	0.06152928664406687
1107	42-43	0.08274930077735831
1107	44-45	0.10275902253385993
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2216	78-79	-0.9037265471921998
2216	80-81	-0.9078068615072503
2216	82-83	-0.9113326057633451
2216	84-85	-0.9812224327622516
2216	86-87	-1.0140401593178545
2216	88-89	-0.995452636982403
2216	90-91	-0.9797620570868588
2216	92-93	-0.9242026259062364
2216	94-95	-1.0511442609701938
2216	96-97	-1.2704258324948192
2216	98-99	-1.37514122971724
2216	100	-1.4326211321649396
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	256.0
3	807.0
4	453.0
5	429.0
6	772.0
7	1587.0
8	3041.0
9	5101.0
10	7089.0
11	9580.0
12	12780.0
13	18582.0
14	22379.0
15	30279.0
16	40448.0
17	59972.0
18	65734.0
19	36256.0
20	22758.0
21	20606.0
22	20459.0
23	21329.0
24	23954.0
25	29066.0
26	36467.0
27	46709.0
28	60107.0
29	75780.0
30	95579.0
31	120640.0
32	152149.0
33	192743.0
34	258068.0
35	395210.0
36	623403.0
37	1164558.0
38	1686232.0
39	856504.0
40	6554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.8214585998492	21.525802432762458	15.875561581981929	23.777177385406414
2	13.631808264866446	21.798304099016455	39.74227317565332	24.82761446046379
3	15.58670422629343	27.802054940432946	31.73354150286276	24.877699330410863
4	10.708066354051182	18.691127370660272	38.65422185238822	31.946584422900326
5	11.57502222406452	39.01123839572861	34.83078461511519	14.582954765091676
6	27.95275704402981	39.88996565141812	17.76226539982842	14.395011904723653
7	24.97291313889487	34.77631650820478	22.395580632412337	17.855189720488013
8	25.793310862698853	34.92139026608101	19.88124194704085	19.40405692417928
9	25.80079750402447	15.97851687386134	21.855144736377046	36.36554088573715
10-11	23.361097989311894	27.773396703589803	28.147247474990923	20.71825783210738
12-13	24.818320421822435	25.840544179216696	28.840879953473575	20.50025544548729
14-15	21.932340683951278	26.86448697228015	27.99619080974613	23.206981534022447
16-17	19.513151426156973	30.02294189659438	28.692424354397676	21.771482322850964
18-19	19.45268796128796	27.38267983201648	33.167821258848214	19.996810947847347
20-21	21.10751684494298	26.20391940132575	33.07713007798317	19.611433675748103
22-23	20.54602677839863	26.29161431908515	33.31572258941395	19.846636313102266
24-25	20.050893393699152	26.530876511705937	33.225828710586356	20.19240138400855
26-27	19.847921573415675	26.681232950218654	33.240840110403866	20.23000536596181
28-29	19.827618684761266	26.61749565038292	33.53602761776901	20.0188580470868
30-31	20.193130320068907	26.8616515784943	33.08132735643877	19.863890744998024
32-33	19.785636062636897	26.71783828021093	33.42959406061405	20.066931596538115
34-35	20.191415487025196	26.549795411172656	33.112744080130284	20.146045021671867
36-37	20.217974462295732	26.769884721166413	32.701985381342844	20.31015543519501
38-39	20.174834994182504	26.782329524513983	32.78517224194234	20.257663239361175
40-41	20.134776908790624	26.653495299115086	32.78928275356824	20.42244503852605
42-43	20.114815847205676	26.889047228490202	32.62427271113367	20.371864213170447
44-45	20.435206849409813	26.914292971416852	32.12049734115768	20.53000283801566
46-47	20.494237711674238	27.086100838579696	31.596725046384037	20.82293640336203
48-49	20.60834638144926	27.223725089558865	30.977710615548688	21.190217913443195
50-51	20.758441858895882	27.36249947785629	30.552090663427766	21.326967999820063
52-53	20.806523391122315	27.61975107520922	30.422492557479714	21.151232976188755
54-55	20.912030090770674	27.848888087736473	30.251148430033126	20.987933391459727
56-57	20.822082986728535	27.52012249224482	30.357077586321164	21.30071693470548
58-59	20.882532585965016	27.12230233743932	30.65843857777865	21.336726498817015
60-61	20.767347968305174	27.498987847746598	30.45528375608095	21.278380427867283
62-63	20.68060274309771	27.320418098359056	30.728500675660804	21.270478482882428
64-65	20.7581361416127	27.33033307697486	30.65043505698661	21.261095724425832
66-67	20.603439647440073	27.29057998524199	30.59995819688816	21.506022170429777
68-69	20.993110960449073	27.409227907036527	30.394730077533826	21.202931054980574
70-71	20.97794838954707	27.324915598263477	30.321052734738362	21.376083277451087
72-73	20.889175216325377	27.300953341837474	30.380220165091686	21.429651276745464
74-75	20.955293145760876	27.430596361562998	30.07101805016336	21.543092442512766
76-77	21.01744419560377	27.32426475077196	29.97721072806784	21.681080325556437
78-79	21.086419764987056	27.377254652089473	30.16464851401678	21.371677068906695
80-81	20.744021129679552	27.552350259140614	30.16859402161165	21.535034589568184
82-83	20.83681529078907	27.51362652653527	30.291700408484516	21.357857774191146
84-85	20.77669885754914	27.303596349291894	30.118398109113333	21.801306684045635
86-87	20.936418247516897	27.41940031516192	29.943948989941955	21.700232447379232
88-89	20.713608657513472	27.401412501084437	30.01670838407434	21.86827045732775
90-91	20.597260467641966	27.384696084133143	29.942115088634765	22.07592835959013
92-93	20.599141364057544	27.503308547841705	29.994999524051085	21.90255056404967
94-95	20.55954611032032	27.30417452549796	30.134647083583694	22.00163228059803
96-97	20.58682032021599	27.538671444642993	29.840526605608495	22.03398162953252
98-99	20.427818835814676	27.479846609794667	29.89539371507588	22.19694083931478
100	20.46770444256403	27.439813084022063	29.897945199867426	22.194537273546484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2662.0
1	1667.0
2	3039.0
3	5639.5
4	3735.0
5	2126.0
6	5807.0
7	17317.0
8	29388.5
9	30564.5
10	19910.5
11	9606.5
12	7417.5
13	7690.0
14	8415.5
15	9252.0
16	9988.0
17	11013.0
18	12295.5
19	13565.5
20	15619.0
21	18536.5
22	22566.5
23	29056.0
24	37331.0
25	46720.0
26	59048.0
27	73948.5
28	91415.0
29	109167.0
30	123174.5
31	136714.5
32	150295.5
33	162397.5
34	172713.0
35	182342.5
36	189554.5
37	195241.0
38	200841.5
39	202077.5
40	204340.0
41	205125.0
42	200912.5
43	202398.0
44	203529.0
45	197965.5
46	194654.5
47	192174.0
48	187771.5
49	186610.0
50	188252.5
51	181423.0
52	167452.5
53	155724.5
54	148556.5
55	143888.5
56	129422.0
57	110500.0
58	100489.0
59	95000.5
60	82520.5
61	66630.0
62	55832.5
63	45566.5
64	35595.0
65	27407.5
66	21071.0
67	16388.0
68	12697.5
69	9519.5
70	6839.5
71	5032.5
72	3811.5
73	2955.5
74	2430.0
75	1771.5
76	1123.5
77	882.0
78	689.0
79	472.0
80	265.5
81	85.5
82	38.5
83	26.5
84	11.0
85	5.0
86	6.5
87	6.5
88	4.5
89	3.5
90	3.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.5
96	2.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04712085624042079
2	0.0
3	9.639452350580456E-5
4	0.06766895550107481
5	0.004996449468384203
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.010924712663991183
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.864000179936444E-4
26-27	0.0
28-29	3.45413709229133E-4
30-31	0.0036790576471382075
32-33	8.836164654698751E-5
34-35	0.0017110027922280309
36-37	0.00402447135636734
38-39	0.0015101808682576048
40-41	9.639452350580455E-4
42-43	0.0016869041613515798
44-45	8.032876958817047E-6
46-47	4.257424788173034E-4
48-49	3.5344658618795006E-4
50-51	0.0012852603134107274
52-53	8.032876958817046E-4
54-55	8.514849576346068E-4
56-57	0.005663178255966018
58-59	0.008619276976810692
60-61	9.639452350580455E-4
62-63	0.0022974028102216754
64-65	0.0010121424968109478
66-67	4.819726175290228E-5
68-69	6.426301567053637E-4
70-71	3.5344658618795006E-4
72-73	0.0
74-75	2.6508493964096254E-4
76-77	0.0
78-79	6.345972797465467E-4
80-81	0.0
82-83	0.0011487014051108377
84-85	6.185315258289125E-4
86-87	8.032876958817047E-6
88-89	0.0
90-91	0.0
92-93	2.0082192397042614E-4
94-95	0.0
96-97	4.418082327349376E-4
98-99	5.301698792819251E-4
100	3.052493244350478E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6224420.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.43893740404552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.15054131921735	30.058103062476448
2	17.572984192129997	15.96995457416953
3	7.035516412906352	9.590591696735599
4	3.2709359163553158	5.94511409423654
5	1.8221164007404949	4.139751653806604
6	1.0851376663825159	2.9584501498516302
7	0.7062699463094952	2.246450911650102
8	0.47515047724921383	1.7272266234583504
9	0.33044409997420143	1.3513525896837508
>10	1.381423605602428	10.686255632890363
>50	0.09314469778656052	2.9607149652828633
>100	0.06789460510908289	6.049775073475249
>500	0.005638955452151908	1.707683522231592
>1k	0.0025534524623595954	1.9159787640363573
>5k	1.4185847004366626E-4	0.5394572153824468
>10k+	1.0639385253274967E-4	2.153139470632539
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54764	0.8798249475453135	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43631	0.7009649091802931	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35218	0.5658037214712375	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9821	0.15778176922508444	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9805	0.1575247171624023	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7906	0.12701585047281513	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.213150783526818E-5	0.0
3	0.0	0.0	0.0	3.213150783526818E-5	0.0
4	0.0	0.0	0.0	1.2852603134107273E-4	0.0
5	0.0	0.0	0.0	1.2852603134107273E-4	0.0
6	0.0	0.0	0.0	1.2852603134107273E-4	0.0
7	0.0	0.0	0.0	1.2852603134107273E-4	0.0
8	0.0	0.0	0.0	1.2852603134107273E-4	0.0
9	0.0	0.0	0.0	1.6065753917634092E-4	0.0
10-11	0.0	0.0	0.0	2.0082192397042616E-4	0.0
12-13	0.0	0.0	0.0	2.1688767788806027E-4	0.0
14-15	0.0	0.0	0.0	2.4098630876451138E-4	0.0
16-17	0.0	0.0	0.0	2.891835705174137E-4	0.0
18-19	0.0	0.0	0.0	2.9721644747623076E-4	0.0
20-21	0.0	0.0	0.0	4.0967672489966936E-4	0.0
22-23	0.0	0.0	0.0	7.068931723759001E-4	0.0
24-25	0.0	0.0	0.0	0.001204931543822557	0.0
26-27	0.0	0.0	0.0	0.0017351014231044822	0.0
28-29	0.0	0.0	0.0	0.003277413799197355	0.0
30-31	0.0	0.0	0.0	0.006956471446335563	0.0
32-33	0.0	0.0	0.0	0.013776383984371234	0.0
34-35	0.0	0.0	0.0	0.024170926769080493	0.0
36-37	0.0	0.0	0.0	0.04103193550563747	0.0
38-39	0.0	0.0	0.0	0.06671304314297558	0.0
40-41	1.606575391763409E-5	0.0	0.0	0.09545467690162296	0.0
42-43	1.606575391763409E-5	0.0	0.0	0.1251602558953284	0.0
44-45	1.606575391763409E-5	0.0	0.0	0.16047278300628814	0.0
46-47	1.606575391763409E-5	0.0	0.0	0.20394671310740597	0.0
48-49	1.606575391763409E-5	0.0	0.0	0.2542566215004772	0.0
50-51	1.606575391763409E-5	8.032876958817046E-6	0.0	0.30684786694985233	0.0
52-53	1.606575391763409E-5	1.606575391763409E-5	0.0	0.35831450962499317	0.0
54-55	1.606575391763409E-5	1.606575391763409E-5	0.0	0.4153881004173883	0.0
56-57	1.606575391763409E-5	1.606575391763409E-5	0.0	0.48415756006182104	0.0
58-59	1.606575391763409E-5	1.606575391763409E-5	0.0	0.5630404117974044	0.0
60-61	1.606575391763409E-5	1.606575391763409E-5	0.0	0.6503738500936633	0.0
62-63	1.606575391763409E-5	1.606575391763409E-5	0.0	0.7400287898310204	0.0
64-65	1.606575391763409E-5	1.606575391763409E-5	0.0	0.8312581734523057	0.0
66-67	1.606575391763409E-5	1.606575391763409E-5	0.0	0.9376134643870433	0.0
68-69	1.606575391763409E-5	1.606575391763409E-5	0.0	1.060693205150038	0.0
70-71	1.606575391763409E-5	1.606575391763409E-5	0.0	1.1967219435706458	0.0
72-73	1.606575391763409E-5	1.606575391763409E-5	0.0	1.341610945276829	0.0
74-75	1.606575391763409E-5	1.606575391763409E-5	0.0	1.4869578209696646	0.0
76-77	1.606575391763409E-5	1.606575391763409E-5	0.0	1.6442817162080965	0.0
78-79	1.606575391763409E-5	1.606575391763409E-5	0.0	1.8291824780461474	0.0
80-81	1.606575391763409E-5	1.606575391763409E-5	0.0	2.0349928186079986	0.0
82-83	1.606575391763409E-5	1.606575391763409E-5	0.0	2.2511655704467244	0.0
84-85	1.606575391763409E-5	1.606575391763409E-5	0.0	2.4717724703667168	0.0
86-87	1.606575391763409E-5	1.606575391763409E-5	0.0	2.7033040829507007	0.0
88	1.606575391763409E-5	1.606575391763409E-5	0.0	2.8934583463198176	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18110	0.0	62.268093	1
GTATCAA	35700	0.0	45.556164	1
GTGGTAT	6045	0.0	37.122574	1
TGGTATC	6160	0.0	35.269207	2
ATCAACG	49335	0.0	32.818317	3
TATCAAC	49690	0.0	32.66903	2
TCAACGC	50090	0.0	32.33304	4
CAACGCA	52565	0.0	30.817398	5
AACGCAG	54385	0.0	29.871086	6
ACGCAGA	60455	0.0	26.646383	7
CGCAGAG	60455	0.0	26.576405	8
GCAGAGT	63410	0.0	25.048798	9
GTACATG	33705	0.0	24.426413	1
CATGGGT	14770	0.0	24.101818	4
TACATGG	33680	0.0	23.987528	2
ACATGGG	34605	0.0	22.626104	3
AGTACTT	45150	0.0	22.410522	12-13
AGAGTAC	62145	0.0	22.35912	10-11
GAGTACT	44320	0.0	22.10915	12-13
CAGAGTA	63120	0.0	21.120087	10-11
>>END_MODULE