FastQCFastQC Report
Wed 25 May 2016
SRR2431446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5310685
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA259730.48907061895028603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71350.044.6676521
GTATCAA114600.034.3319321
ATCAACG129550.029.0618923
TCAACGC129800.029.0053714
TATCAAC141450.027.148632
GTACATG256050.027.0601431
TACATGG254650.026.2842222
CATGGGT115650.026.13284
CAACGCA147750.025.6087885
ACATGGG262800.024.6463533
AACGCAG157450.024.1195326
GTGGTAT38750.022.68441
TGGTATC37950.022.2940772
ACGCAGA170950.021.8296857
ATGGGTG69000.021.6620045
CGCAGAG169500.021.544848
GCAGAGT186250.018.7745119
ATGGGTA54500.018.714745
GAGTACA186900.017.7563131
CATGGGG101800.017.6836384
Produced by FastQC (version 0.11.5)