##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431446_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5310685
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.913602670841897	31.0	30.0	34.0	26.0	34.0
2	29.83894036268391	31.0	30.0	34.0	25.0	34.0
3	29.970082955400294	31.0	30.0	34.0	25.0	34.0
4	33.292929819787844	37.0	35.0	37.0	26.0	37.0
5	33.38831958589146	37.0	35.0	37.0	27.0	37.0
6	33.404273083415795	37.0	35.0	37.0	27.0	37.0
7	33.19005024775523	37.0	35.0	37.0	26.0	37.0
8	33.160212665597754	37.0	35.0	37.0	26.0	37.0
9	34.41800652834804	39.0	35.0	39.0	25.0	39.0
10-11	34.39004977700617	39.0	35.0	39.0	25.0	39.0
12-13	34.36727286592972	39.0	35.0	39.0	25.0	39.0
14-15	35.4969071786408	40.0	36.0	41.0	24.5	41.0
16-17	35.493750712007966	40.0	36.0	41.0	25.0	41.0
18-19	35.47353571149485	40.0	36.0	41.0	25.0	41.0
20-21	35.58663807023011	40.0	36.0	41.0	25.0	41.0
22-23	35.54146583726958	40.0	36.0	41.0	25.0	41.0
24-25	35.587051199609846	40.0	35.5	41.0	25.0	41.0
26-27	35.58840752181686	40.0	35.0	41.0	26.0	41.0
28-29	35.490596222521205	40.0	35.0	41.0	25.5	41.0
30-31	35.46317932620745	40.0	35.0	41.0	26.0	41.0
32-33	35.4136387302203	40.0	35.0	41.0	25.5	41.0
34-35	35.309779717682375	40.0	35.0	41.0	25.0	41.0
36-37	35.17884265400791	40.0	35.0	41.0	25.0	41.0
38-39	35.06441466590468	40.0	35.0	41.0	25.0	41.0
40-41	34.97939550171023	39.0	35.0	41.0	24.0	41.0
42-43	34.74084209475802	39.0	34.0	41.0	24.0	41.0
44-45	34.59819109964157	39.0	34.0	41.0	23.0	41.0
46-47	34.455742809072646	39.0	34.0	41.0	23.0	41.0
48-49	34.31523974779148	39.0	33.0	40.5	22.5	41.0
50-51	34.14488629997825	38.5	33.0	40.0	22.0	41.0
52-53	34.051342435109596	38.0	33.0	40.0	21.5	41.0
54-55	33.98025593685183	38.0	33.0	40.0	21.0	41.0
56-57	33.82007537634034	38.0	33.0	40.0	19.5	41.0
58-59	33.60753330314263	38.0	33.0	40.0	18.5	41.0
60-61	33.392681923330045	38.0	33.0	40.0	18.0	41.0
62-63	33.13202854245733	37.0	32.0	40.0	15.5	41.0
64-65	32.998586999605514	37.0	32.0	40.0	13.0	41.0
66-67	32.754573468394376	36.5	32.0	40.0	11.5	41.0
68-69	32.496913298378644	36.0	32.0	39.5	8.0	41.0
70-71	32.207299529156785	36.0	31.5	39.0	7.5	41.0
72-73	31.899169975248014	35.0	31.0	39.0	4.0	41.0
74-75	31.561103699428607	35.0	31.0	38.5	2.0	40.0
76-77	31.231646388366094	35.0	31.0	37.0	2.0	39.5
78-79	30.886352043098018	35.0	31.0	37.0	2.0	39.0
80-81	30.5788281549367	35.0	31.0	36.5	2.0	39.0
82-83	30.26452764568036	35.0	30.0	36.0	2.0	38.5
84-85	29.962229354593617	35.0	30.0	36.0	2.0	37.0
86-87	29.690795537675463	35.0	30.0	35.0	2.0	37.0
88-89	29.410461927227843	34.0	30.0	35.0	2.0	36.5
90-91	29.17652666275631	34.0	30.0	35.0	2.0	36.0
92-93	28.972712653829028	34.0	29.0	35.0	2.0	36.0
94-95	28.72573867589586	34.0	29.0	35.0	2.0	36.0
96-97	28.6213152352286	34.0	29.0	35.0	2.0	35.0
98-99	28.450794294897925	34.0	29.0	35.0	2.0	35.0
100	28.300945734872244	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4057138753352909
1101	2	0.5035780656751889
1101	3	0.5484558003340254
1101	4	0.5054850587600868
1101	5	0.4319051611297482
1101	6	0.5080121509327853
1101	7	0.512706824433323
1101	8	0.5590299953746438
1101	9	0.7104403945538493
1101	10-11	0.7314181206667243
1101	12-13	0.6908036753885085
1101	14-15	0.7888832207500158
1101	16-17	0.7469753408135063
1101	18-19	0.7427220335091462
1101	20-21	0.7394010268165516
1101	22-23	0.7522046939432769
1101	24-25	0.6689614037808411
1101	26-27	0.757875006295869
1101	28-29	0.7964040647691988
1101	30-31	0.7648053339704504
1101	32-33	0.7371640869964722
1101	34-35	0.7270534181461343
1101	36-37	0.743779277093239
1101	38-39	0.7010086940870437
1101	40-41	0.7360973535001065
1101	42-43	0.7548480046230708
1101	44-45	0.7277285338338402
1101	46-47	0.6408509625801884
1101	48-49	0.6483416372404989
1101	50-51	0.5898711750471435
1101	52-53	0.6364713367437531
1101	54-55	0.7106717386222527
1101	56-57	0.6659472297144262
1101	58-59	0.7342147906442165
1101	60-61	0.7269991696929949
1101	62-63	0.6884230104422429
1101	64-65	0.6389761643721314
1101	66-67	0.6927041377000407
1101	68-69	0.690425843530484
1101	70-71	0.6607233308864906
1101	72-73	0.667007507567174
1101	74-75	0.6496668275147925
1101	76-77	0.6735746679799917
1101	78-79	0.6206335895786204
1101	80-81	0.6163288261752697
1101	82-83	0.6349025631398888
1101	84-85	0.6065357449511737
1101	86-87	0.575579103332565
1101	88-89	0.6431045170293608
1101	90-91	0.6316346689445353
1101	92-93	0.6546753081072154
1101	94-95	0.6289870146283043
1101	96-97	0.7264930467613446
1101	98-99	0.6943924115850884
1101	100	0.679043446564318
1102	1	0.5336319646538001
1102	2	0.6311383716427876
1102	3	0.6186732149081031
1102	4	0.6611814335960062
1102	5	0.5038089867494264
1102	6	0.5904122011159316
1102	7	0.5028756580173592
1102	8	0.6207612804493579
1102	9	0.7712903936329951
1102	10-11	0.7194125765653112
1102	12-13	0.7646026622817601
1102	14-15	0.8889679117214442
1102	16-17	0.8055224233023139
1102	18-19	0.7394960981869616
1102	20-21	0.8140222922818907
1102	22-23	0.7874888829725251
1102	24-25	0.7850180835873459
1102	26-27	0.8703552546470235
1102	28-29	0.9046476181952059
1102	30-31	0.9332185615203841
1102	32-33	0.9509320576908422
1102	34-35	0.8899929931597512
1102	36-37	0.8578197516507089
1102	38-39	0.8349419575738679
1102	40-41	0.8483718980611101
1102	42-43	0.8468755945487274
1102	44-45	0.8124493465015945
1102	46-47	0.8231087441170857
1102	48-49	0.7563065454617117
1102	50-51	0.7507070462708967
1102	52-53	0.7147865811601548
1102	54-55	0.7644209931104555
1102	56-57	0.7014788552701035
1102	58-59	0.6724667155428747
1102	60-61	0.670595152355439
1102	62-63	0.6687634817178676
1102	64-65	0.6104058915677797
1102	66-67	0.6999639721206066
1102	68-69	0.6685815861557884
1102	70-71	0.7131520238164981
1102	72-73	0.7106282217657558
1102	74-75	0.6630109348524371
1102	76-77	0.6490406483396072
1102	78-79	0.6453718936460682
1102	80-81	0.6299393583478015
1102	82-83	0.6950936488750941
1102	84-85	0.6802446369077337
1102	86-87	0.6443986183701256
1102	88-89	0.6666844650432822
1102	90-91	0.6970066895456526
1102	92-93	0.656071009361888
1102	94-95	0.6682614659960109
1102	96-97	0.7430595929413961
1102	98-99	0.788281998154396
1102	100	0.71205509683492
1103	1	0.28876426481490114
1103	2	0.3312801308565909
1103	3	0.38595002269098444
1103	4	0.4291145290679452
1103	5	0.3121474817861625
1103	6	0.29363985032548356
1103	7	0.3436306842613277
1103	8	0.41199595867341543
1103	9	0.4984622329395094
1103	10-11	0.6150380886770961
1103	12-13	0.5421634760221679
1103	14-15	0.6199444961693601
1103	16-17	0.553856631831195
1103	18-19	0.4909886555935188
1103	20-21	0.5315161514670947
1103	22-23	0.46280246043585294
1103	24-25	0.47720303775031425
1103	26-27	0.5028333943455294
1103	28-29	0.5798188597243339
1103	30-31	0.44620002622990995
1103	32-33	0.5283144804134139
1103	34-35	0.4940137033231835
1103	36-37	0.4940070082021961
1103	38-39	0.540469329645795
1103	40-41	0.4917744296825717
1103	42-43	0.4926096357146932
1103	44-45	0.4161220964395511
1103	46-47	0.40742214725661086
1103	48-49	0.36363406809119425
1103	50-51	0.31620304374754227
1103	52-53	0.3426948655842992
1103	54-55	0.4624406392247664
1103	56-57	0.40789872729786225
1103	58-59	0.4217568968010639
1103	60-61	0.4528567905478198
1103	62-63	0.3705462535440418
1103	64-65	0.417846010471429
1103	66-67	0.4394669065376746
1103	68-69	0.424350692927554
1103	70-71	0.39330731148245235
1103	72-73	0.351663877805823
1103	74-75	0.3299323420461704
1103	76-77	0.3523394403685671
1103	78-79	0.32126116044701547
1103	80-81	0.3075531448622044
1103	82-83	0.41683268148900154
1103	84-85	0.4196640306934576
1103	86-87	0.3853048358887392
1103	88-89	0.4391706841921348
1103	90-91	0.45915897618668566
1103	92-93	0.4483244960138819
1103	94-95	0.47142317460528105
1103	96-97	0.4033009517670685
1103	98-99	0.44382413359778283
1103	100	0.49873581312566984
1104	1	0.39065349272823013
1104	2	0.4113620793616306
1104	3	0.38055632085052693
1104	4	0.44593353775503886
1104	5	0.49552177915310835
1104	6	0.5014622362319017
1104	7	0.45566428701535244
1104	8	0.4336341719262222
1104	9	0.5530881517267972
1104	10-11	0.5841730287569078
1104	12-13	0.5364303199009157
1104	14-15	0.5967462936201215
1104	16-17	0.5970775761546463
1104	18-19	0.5285099796564552
1104	20-21	0.5406472891399048
1104	22-23	0.6033155920326934
1104	24-25	0.5733155169785604
1104	26-27	0.6409800296086843
1104	28-29	0.7125652319592817
1104	30-31	0.6654412388976638
1104	32-33	0.6748867675167816
1104	34-35	0.6157612134740873
1104	36-37	0.5674076886809019
1104	38-39	0.5970180089630475
1104	40-41	0.6280478012375426
1104	42-43	0.5734927384956734
1104	44-45	0.5268301851739992
1104	46-47	0.492103439711002
1104	48-49	0.4841421787979172
1104	50-51	0.4786988955602638
1104	52-53	0.41750435229293004
1104	54-55	0.508757442274117
1104	56-57	0.5213016556119143
1104	58-59	0.5369965927233409
1104	60-61	0.5104592258141665
1104	62-63	0.527454674107517
1104	64-65	0.5542777588797065
1104	66-67	0.5303701514351147
1104	68-69	0.5295170678606169
1104	70-71	0.4915201737040178
1104	72-73	0.5603956005386941
1104	74-75	0.6337286598751604
1104	76-77	0.5634731741955008
1104	78-79	0.5772463707999123
1104	80-81	0.5572935562134766
1104	82-83	0.5258694434914304
1104	84-85	0.49423681180803314
1104	86-87	0.5651175275226663
1104	88-89	0.552610238370054
1104	90-91	0.5214087031674737
1104	92-93	0.5488694671327323
1104	94-95	0.64609087315457
1104	96-97	0.6264925996167499
1104	98-99	0.6050428733297046
1104	100	0.5579112221897731
1105	1	0.2741781746128531
1105	2	0.31091066475670104
1105	3	0.3947138230626379
1105	4	0.306411132880001
1105	5	0.3081066685985263
1105	6	0.373476312291146
1105	7	0.3891890113181944
1105	8	0.3900706688945945
1105	9	0.47318369450599107
1105	10-11	0.4951628662018379
1105	12-13	0.47402422321624016
1105	14-15	0.5573501677601982
1105	16-17	0.5715541142177756
1105	18-19	0.5282919500502388
1105	20-21	0.4750182567586876
1105	22-23	0.5463839897017237
1105	24-25	0.5745916688662973
1105	26-27	0.6430553405977975
1105	28-29	0.6730556373840599
1105	30-31	0.6814606884959247
1105	32-33	0.6491919360061615
1105	34-35	0.6762224351694712
1105	36-37	0.5689228671894568
1105	38-39	0.5208187825015429
1105	40-41	0.5381400693308152
1105	42-43	0.5485692821580059
1105	44-45	0.49409626358490755
1105	46-47	0.4500759001114858
1105	48-49	0.4446132839365404
1105	50-51	0.45865374561612526
1105	52-53	0.4555348238392227
1105	54-55	0.5181602871787092
1105	56-57	0.5106158719057632
1105	58-59	0.5200469117507112
1105	60-61	0.4490047817005731
1105	62-63	0.5020054440404706
1105	64-65	0.5580534038698204
1105	66-67	0.5704076406502878
1105	68-69	0.5252464023443366
1105	70-71	0.5135237724650707
1105	72-73	0.5212849492409894
1105	74-75	0.5541448843319756
1105	76-77	0.4681308210537125
1105	78-79	0.4753355576343168
1105	80-81	0.5343393653366171
1105	82-83	0.5249638146796229
1105	84-85	0.5182809386249119
1105	86-87	0.48040793532286585
1105	88-89	0.5536278512597583
1105	90-91	0.5129163651689836
1105	92-93	0.5176799926180173
1105	94-95	0.5391628339533554
1105	96-97	0.5302940348793506
1105	98-99	0.5796702081341039
1105	100	0.5572253197126358
1106	1	0.2276559457781211
1106	2	0.31206141225219497
1106	3	0.3194053323638748
1106	4	0.28084454865499
1106	5	0.226404890899083
1106	6	0.34026767855076656
1106	7	0.303740260761451
1106	8	0.3257924619432231
1106	9	0.47107857982461354
1106	10-11	0.5410692497360756
1106	12-13	0.46075662025333486
1106	14-15	0.5254357632591606
1106	16-17	0.3903328111376396
1106	18-19	0.44796559597716623
1106	20-21	0.4557309538734273
1106	22-23	0.4367801564799194
1106	24-25	0.47728015413920843
1106	26-27	0.47843367141420856
1106	28-29	0.5650583001761831
1106	30-31	0.5279359611919503
1106	32-33	0.44639248024840583
1106	34-35	0.40022866475799646
1106	36-37	0.4032691086865867
1106	38-39	0.5077612965709548
1106	40-41	0.4787905817334135
1106	42-43	0.4434514559520366
1106	44-45	0.33932204276547395
1106	46-47	0.36860196621289276
1106	48-49	0.3288281767795951
1106	50-51	0.34622981804832165
1106	52-53	0.42496603183960957
1106	54-55	0.45564695974029235
1106	56-57	0.3984958502912903
1106	58-59	0.45889543112237163
1106	60-61	0.5161746525415722
1106	62-63	0.4954087408478287
1106	64-65	0.5266143097722633
1106	66-67	0.5493496787772756
1106	68-69	0.5290605663552626
1106	70-71	0.5359389667058281
1106	72-73	0.5253317466306946
1106	74-75	0.5229259128396961
1106	76-77	0.5204173183456717
1106	78-79	0.576487476253952
1106	80-81	0.4251974941397414
1106	82-83	0.4218774015258475
1106	84-85	0.509119938051775
1106	86-87	0.5185515704550454
1106	88-89	0.5029357960837011
1106	90-91	0.45216352362433554
1106	92-93	0.5011650482059054
1106	94-95	0.535135651750835
1106	96-97	0.582145807539451
1106	98-99	0.6998119275569401
1106	100	0.652167379221396
1107	1	0.17300665274845528
1107	2	0.12883007895167609
1107	3	0.2407064308508282
1107	4	0.24595984099765644
1107	5	0.18620894709545155
1107	6	0.17092462153483723
1107	7	0.20793635467551752
1107	8	0.3363443592633075
1107	9	0.3409007141853735
1107	10-11	0.3434786893523025
1107	12-13	0.3381658229192368
1107	14-15	0.36131155024292383
1107	16-17	0.34865051967283023
1107	18-19	0.3575200721301073
1107	20-21	0.3095162010485524
1107	22-23	0.37424645759593034
1107	24-25	0.3737827701965557
1107	26-27	0.41124799061331885
1107	28-29	0.4660459461255684
1107	30-31	0.3804590282882785
1107	32-33	0.38302001415496534
1107	34-35	0.4098216044927412
1107	36-37	0.36473403515059033
1107	38-39	0.3957328948243841
1107	40-41	0.4014286863306751
1107	42-43	0.43665607530208916
1107	44-45	0.3934880901945519
1107	46-47	0.36135179618162283
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2216	98-99	-2.0330147358816824
2216	100	-2.111750809178183
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	175858.0
3	35573.0
4	22961.0
5	19503.0
6	15280.0
7	15066.0
8	17020.0
9	17744.0
10	18359.0
11	18987.0
12	19957.0
13	20888.0
14	21887.0
15	21880.0
16	21838.0
17	21478.0
18	21152.0
19	20575.0
20	20680.0
21	21700.0
22	26201.0
23	37236.0
24	54352.0
25	68533.0
26	74436.0
27	66243.0
28	68906.0
29	82635.0
30	95955.0
31	112899.0
32	141812.0
33	183108.0
34	248653.0
35	363948.0
36	579159.0
37	933465.0
38	1159593.0
39	444222.0
40	943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76766409869914	21.549025279677952	14.462594980523672	22.220715641099233
2	15.258627865048528	21.833405080773304	39.77054754407195	23.13741951010622
3	17.992426667711197	27.44824500415426	30.319279154956526	24.24004917317802
4	12.205348813297725	18.755496433132677	38.63824421469223	30.40091053887737
5	12.34524541720039	40.17732348910075	34.52002210093741	12.957408992761454
6	28.75837499251434	39.55858907293461	18.277473685320615	13.405562249230437
7	25.72062652122536	34.86516235034909	22.19521587865035	17.2189952497752
8	24.172158493016234	37.4975990129401	20.428294662690508	17.901947831353162
9	24.86342363351605	18.87434447016128	21.35931782150456	34.902914074818106
10-11	22.831270997700173	29.275574753931107	28.42347369870046	19.469680549668265
12-13	23.217586524816284	27.361750110726458	28.132146375855307	21.288516988601955
14-15	21.173444407535207	29.110511900968756	25.700249175003258	24.01579451649278
16-17	20.44237305689725	32.09168714515719	26.542712952762948	20.92322684518261
18-19	20.45842718972405	30.993370013433967	28.289176106287677	20.2590266905543
20-21	22.14354002654759	29.83799152729244	27.703111762571364	20.315356683588607
22-23	21.742511406840524	29.864869190891095	27.716946525786145	20.675672876482228
24-25	21.3178780673404	29.918238151650066	27.81571260895789	20.948171172051644
26-27	21.036189169141842	29.97370631709357	27.879956711366994	21.1101478023976
28-29	20.988483890356324	29.933815256173386	28.14985829796158	20.927842555508715
30-31	21.366181480561906	30.265581746434588	27.609811359198062	20.758425413805437
32-33	20.910503307967247	30.056521719071494	28.036329970238793	20.99664500272247
34-35	21.292229286793223	29.771318239281975	27.956192483191423	20.98025999073338
36-37	21.33308250366052	29.923302048357588	27.67618265861187	21.06743278937002
38-39	21.165383110729476	30.01147267859505	27.83757662379359	20.985567586881885
40-41	21.12914756634962	29.66380334918981	28.072421754960995	21.13462732949957
42-43	21.0330004132431	29.717630396840484	28.094900350413955	21.154468839502464
44-45	21.201024572510256	29.762108423724097	27.947319535844738	21.08954746792091
46-47	21.216099375766124	29.85317854233824	27.814746434351495	21.11597564754414
48-49	21.000432820015888	29.69243227807288	27.922342466742673	21.384792435168563
50-51	21.03553288977832	29.657711299448593	27.86720277412542	21.439553036647663
52-53	21.112291050929056	29.631151063984234	27.889047703202003	21.367510181884704
54-55	21.17698056775863	29.894283537726402	27.75636884698808	21.172367047526887
56-57	21.116467216370722	29.63247999808683	27.734909791435154	21.5161429941073
58-59	21.187704786143033	29.291400989780662	27.972230102208417	21.548664121867887
60-61	21.17174223727076	29.69716051320403	27.741862166215565	21.389235083309647
62-63	20.985114758352204	29.61021676091184	27.88543550797251	21.519232972763444
64-65	21.126115675898387	29.406333690992376	27.88313173741323	21.584418895696004
66-67	20.920390803322856	29.38914878566687	27.831164709257433	21.859295701752842
68-69	21.25903947905711	29.585768787998244	27.75382167994894	21.40137005299571
70-71	21.166975899079198	29.38411561632785	27.781222760133257	21.667685724459705
72-73	21.04194282683123	29.347343556943862	28.02911863845377	21.581594977771136
74-75	21.08631374944544	29.37491855843343	27.88619496715598	21.652572724965143
76-77	21.069119059475458	29.292692486689514	27.706713509109242	21.93147494472579
78-79	21.11362508886824	29.309058671257045	27.9716216675528	21.60569457232192
80-81	20.916909895490065	29.289210055550324	28.014744374352695	21.779135674606913
82-83	20.877103869099596	29.27526584789579	28.206083961283113	21.6415463217215
84-85	20.895313848871503	29.026554288345697	28.061658500725017	22.01647336205779
86-87	21.19396163997875	29.144344093424053	27.84753683259892	21.814157433998275
88-89	21.03074339566648	29.00376303712346	28.01787706257141	21.947616504638646
90-91	20.960119760760936	28.93239213175567	27.94743519431701	22.160052913166385
92-93	21.01962695408288	29.019874384706977	27.898075906781926	22.062422754428212
94-95	20.875189079352786	28.77371129934258	28.110669583348475	22.240430037956155
96-97	21.004331133526996	28.915697021383007	27.91306437159209	22.16690747349791
98-99	20.865206262222593	28.926959660822167	27.964875574946102	22.242958502009138
100	20.877998296600524	28.874146346607333	27.880069830716437	22.3677855260757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20306.0
1	15207.5
2	8850.0
3	6384.5
4	4403.0
5	3306.5
6	2834.0
7	2755.5
8	2873.0
9	2874.0
10	2529.0
11	2181.0
12	2188.0
13	2245.0
14	2303.5
15	2469.5
16	2773.0
17	3416.0
18	4220.5
19	5281.0
20	7025.0
21	9292.0
22	12529.5
23	17849.0
24	24568.0
25	32596.5
26	43511.0
27	57644.0
28	75220.5
29	92257.5
30	105567.5
31	117763.5
32	130268.0
33	143215.5
34	155169.0
35	164775.5
36	171539.5
37	177130.5
38	181215.5
39	182595.0
40	184403.0
41	184458.5
42	181415.0
43	179543.0
44	177494.5
45	174352.5
46	172161.0
47	168199.5
48	163988.0
49	162637.5
50	161186.5
51	153531.0
52	143483.5
53	134653.5
54	127336.5
55	122253.0
56	111104.0
57	97050.5
58	86610.0
59	78536.0
60	67955.0
61	55563.0
62	46570.5
63	38844.5
64	31198.0
65	25234.5
66	20543.0
67	16072.5
68	12414.0
69	9529.0
70	7191.0
71	5568.0
72	4429.5
73	3514.5
74	2740.0
75	2058.5
76	1510.5
77	1107.0
78	818.0
79	601.0
80	416.0
81	281.0
82	211.0
83	157.0
84	123.0
85	98.5
86	76.0
87	63.0
88	53.5
89	43.5
90	33.0
91	30.5
92	34.5
93	29.5
94	21.0
95	20.5
96	21.5
97	21.0
98	21.5
99	24.5
100	39.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012220645735907892
2	0.007362515381725709
3	0.008906572316000666
4	0.007965074185345205
5	0.009471471194393944
6	0.009998710147561003
7	0.007720284671374785
8	0.006948256204237306
9	0.005385369307349241
10-11	0.005310049456896803
12-13	0.005667818746545879
14-15	0.006797616503332432
16-17	0.00442504121408067
18-19	0.005027600017700164
20-21	0.00418025170011025
22-23	0.005178239718605039
24-25	0.004933450204634619
26-27	0.005347709382123022
28-29	0.014602636006466208
30-31	0.004679245709357644
32-33	0.004679245709357644
34-35	0.004264986531869241
36-37	0.005809043466144198
38-39	0.004566265933678989
40-41	0.004453286158000333
42-43	0.004735735597196972
44-45	0.0033423183638268887
46-47	0.004811055447649409
48-49	0.003078698887243359
50-51	0.0034364681768924345
52-53	0.0028339093732729394
54-55	0.0038883872796070564
56-57	0.0024573101210107547
58-59	0.002156030719201007
60-61	0.0031163588124695782
62-63	0.002946889148951595
64-65	0.004274401513175796
66-67	0.00257028989668941
68-69	0.0030222089994040315
70-71	0.004217911625336468
72-73	0.0025326299714631914
74-75	0.002429065177091091
76-77	0.0021936906444272254
78-79	0.0029092292237253765
80-81	0.0034835430834252077
82-83	0.0015534719155815117
84-85	0.002099540831361679
86-87	0.00195831611176336
88-89	0.0015252269716618477
90-91	0.00183592135477815
92-93	0.001986561055683024
94-95	0.0015817168595011755
96-97	0.0012427775324652093
98-99	0.0014781520651290746
100	0.002410235214477982
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5310685.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.04358343284955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6234338706069	42.73367926481453
2	15.252461189707594	17.70615007242185
3	5.268195059075742	9.173547583559658
4	2.286574501740911	5.308839114688998
5	1.1471868732987152	3.3293418496691896
6	0.6451345155617443	2.2467551447651446
7	0.39796602998364927	1.6169562125357095
8	0.2788428216726037	1.294802926752397
9	0.17935145308002173	0.9369180927587739
>10	0.837051351202787	8.386005475237155
>50	0.051420217751008614	2.049750356331722
>100	0.02961487633063223	3.338545783420094
>500	0.0021161273124434016	0.8585451290187972
>1k	6.185570414528191E-4	0.5293653308182876
>5k	0.0	0.0
>10k+	3.255563375989244E-5	0.4907976632074438
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25973	0.48907061895028603	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.765992522621846E-5	0.0
5	0.0	0.0	0.0	3.765992522621846E-5	0.0
6	0.0	0.0	0.0	3.765992522621846E-5	0.0
7	0.0	0.0	0.0	3.765992522621846E-5	0.0
8	0.0	0.0	0.0	3.765992522621846E-5	0.0
9	0.0	0.0	0.0	5.648988783932769E-5	0.0
10-11	0.0	0.0	0.0	5.648988783932769E-5	0.0
12-13	0.0	0.0	0.0	6.590486914588231E-5	0.0
14-15	0.0	0.0	0.0	1.1297977567865538E-4	0.0
16-17	0.0	0.0	0.0	1.4122471959831923E-4	0.0
18-19	0.0	0.0	0.0	1.6005468221142849E-4	0.0
20-21	0.0	0.0	0.0	2.0712958874420157E-4	0.0
22-23	0.0	0.0	0.0	4.7074906532773076E-4	0.0
24-25	0.0	0.0	0.0	0.0010733078689472261	0.0
26-27	0.0	0.0	0.0	0.00208071086874857	0.0
28-29	0.0	0.0	0.0	0.005093504886846047	0.0
30-31	0.0	0.0	0.0	0.013256293679628897	0.0
32-33	0.0	0.0	0.0	0.029789000853938805	0.0
34-35	0.0	0.0	0.0	0.05566136948435089	0.0
36-37	0.0	0.0	0.0	0.09776516588726314	0.0
38-39	0.0	0.0	0.0	0.15433037357704327	0.0
40-41	0.0	0.0	0.0	0.21281623745336053	0.0
42-43	0.0	0.0	0.0	0.27353345189933126	0.0
44-45	0.0	0.0	0.0	0.35283583944444075	0.0
46-47	0.0	0.0	0.0	0.45295475065834256	0.0
48-49	0.0	1.882996261310923E-5	0.0	0.5589486102075344	0.0
50-51	0.0	1.882996261310923E-5	0.0	0.6680494135878894	0.0
52-53	0.0	1.882996261310923E-5	0.0	0.7761898888749756	0.0
54-55	0.0	1.882996261310923E-5	0.0	0.9000722129066212	0.0
56-57	0.0	1.882996261310923E-5	0.0	1.0493655714846577	0.0
58-59	0.0	1.882996261310923E-5	0.0	1.2159354207602222	0.0
60-61	0.0	1.882996261310923E-5	0.0	1.3918355165105818	0.0
62-63	0.0	1.882996261310923E-5	0.0	1.5704753718211493	0.0
64-65	0.0	1.882996261310923E-5	0.0	1.7617501320451128	0.0
66-67	0.0	1.882996261310923E-5	0.0	1.994234265447866	0.0
68-69	0.0	1.882996261310923E-5	0.0	2.2591530094517	0.0
70-71	0.0	1.882996261310923E-5	0.0	2.5482117655255396	0.0
72-73	0.0	1.882996261310923E-5	0.0	2.845226180803418	0.0
74-75	0.0	1.882996261310923E-5	0.0	3.1484074088370897	0.0
76-77	9.414981306554615E-6	3.765992522621846E-5	0.0	3.4776869650525306	0.0
78-79	1.882996261310923E-5	3.765992522621846E-5	0.0	3.859502116958547	0.0
80-81	1.882996261310923E-5	3.765992522621846E-5	0.0	4.280144651772794	0.0
82-83	1.882996261310923E-5	4.7074906532773074E-5	0.0	4.718694481032108	0.0
84-85	1.882996261310923E-5	5.648988783932769E-5	0.0	5.14648298665803	0.0
86-87	1.882996261310923E-5	5.648988783932769E-5	0.0	5.603184899876382	0.0
88	1.882996261310923E-5	7.531985045243692E-5	0.0	5.973767979083678	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7135	0.0	44.667652	1
GTATCAA	11460	0.0	34.331932	1
ATCAACG	12955	0.0	29.061892	3
TCAACGC	12980	0.0	29.005371	4
TATCAAC	14145	0.0	27.14863	2
GTACATG	25605	0.0	27.060143	1
TACATGG	25465	0.0	26.284222	2
CATGGGT	11565	0.0	26.1328	4
CAACGCA	14775	0.0	25.608788	5
ACATGGG	26280	0.0	24.646353	3
AACGCAG	15745	0.0	24.119532	6
GTGGTAT	3875	0.0	22.6844	1
TGGTATC	3795	0.0	22.294077	2
ACGCAGA	17095	0.0	21.829685	7
ATGGGTG	6900	0.0	21.662004	5
CGCAGAG	16950	0.0	21.54484	8
GCAGAGT	18625	0.0	18.774511	9
ATGGGTA	5450	0.0	18.71474	5
GAGTACA	18690	0.0	17.756313	1
CATGGGG	10180	0.0	17.683638	4
>>END_MODULE