FastQCFastQC Report
Wed 25 May 2016
SRR2431446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5310685
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356430.6711563574190523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319190.6010335766478336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218350.41115223365724No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79730.15013129191431993No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65560.12344923489154412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142200.062.1984981
GTATCAA272050.043.5786171
GTGGTAT49350.037.179121
TGGTATC49500.037.0496942
ATCAACG359850.032.6434563
TATCAAC364250.032.365332
TCAACGC364300.032.3092544
CAACGCA382550.030.7182885
AACGCAG396450.029.6760036
ACGCAGA444650.026.3217077
CGCAGAG442400.026.306838
GCAGAGT468850.024.5119539
CATGGGT113500.024.3202174
GTACATG240250.023.635541
TACATGG238250.023.4481582
ACATGGG244050.022.274313
GAGTACT333850.022.18545212-13
CAGAGTA467250.021.24098810-11
AGAGTAC450600.021.21221410-11
AGTACTT338950.020.90880212-13
Produced by FastQC (version 0.11.5)