##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431446_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5310685
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26905945278246	33.0	31.0	34.0	30.0	34.0
2	32.380240213833055	34.0	31.0	34.0	31.0	34.0
3	32.48294090122084	34.0	31.0	34.0	31.0	34.0
4	36.04275568970858	37.0	35.0	37.0	35.0	37.0
5	35.96253496488683	37.0	35.0	37.0	35.0	37.0
6	36.01054195456895	37.0	35.0	37.0	35.0	37.0
7	35.942906800158546	37.0	35.0	37.0	35.0	37.0
8	35.92343417092145	37.0	35.0	37.0	35.0	37.0
9	37.603776725601314	39.0	37.0	39.0	35.0	39.0
10-11	37.54307965921534	39.0	37.0	39.0	35.0	39.0
12-13	37.53629870722892	39.0	37.0	39.0	35.0	39.0
14-15	38.87043196875733	40.0	38.0	41.0	35.0	41.0
16-17	38.923411292516874	40.0	38.0	41.0	35.0	41.0
18-19	39.014107784588994	40.5	38.5	41.0	35.0	41.0
20-21	39.04275616045764	41.0	39.0	41.0	35.0	41.0
22-23	38.99740419550397	40.5	39.0	41.0	35.0	41.0
24-25	38.950634522665155	41.0	38.0	41.0	35.0	41.0
26-27	38.8194633084056	40.0	38.0	41.0	35.0	41.0
28-29	38.72510034016328	40.0	38.0	41.0	34.5	41.0
30-31	38.53714982153903	40.0	38.0	41.0	34.0	41.0
32-33	38.32916130405023	40.0	38.0	41.0	34.0	41.0
34-35	38.181431208968334	40.0	38.0	41.0	34.0	41.0
36-37	38.035071276115985	40.0	37.0	41.0	33.0	41.0
38-39	37.91667204889765	40.0	37.0	41.0	33.0	41.0
40-41	37.778860636622206	40.0	37.0	41.0	33.0	41.0
42-43	37.636138370097264	40.0	37.0	41.0	33.0	41.0
44-45	37.398623058983915	40.0	37.0	41.0	32.5	41.0
46-47	37.04277329572362	40.0	37.0	41.0	31.0	41.0
48-49	36.76016954498337	40.0	37.0	41.0	31.0	41.0
50-51	36.622833589263905	40.0	36.0	41.0	30.5	41.0
52-53	36.48755377131199	40.0	36.0	41.0	30.0	41.0
54-55	36.334118856607006	40.0	35.5	41.0	30.0	41.0
56-57	36.11911467164782	39.5	35.0	41.0	30.0	41.0
58-59	35.95406223490943	39.0	35.0	41.0	29.0	41.0
60-61	35.75388786945564	39.0	35.0	41.0	29.0	41.0
62-63	35.541032936429104	39.0	35.0	41.0	28.5	41.0
64-65	35.30839731597713	38.0	35.0	41.0	28.0	41.0
66-67	35.05250490285151	37.5	35.0	40.0	28.0	41.0
68-69	34.77050154546918	37.0	35.0	40.0	28.0	41.0
70-71	34.446216542687054	36.5	34.0	39.5	28.0	41.0
72-73	34.12916676473939	36.0	34.0	39.0	27.0	41.0
74-75	33.822922560837256	36.0	34.0	39.0	27.0	41.0
76-77	33.465252881690404	35.0	34.0	38.5	26.0	40.0
78-79	33.151622624953276	35.0	34.0	37.0	26.0	39.0
80-81	32.84658495090558	35.0	34.0	37.0	26.0	39.0
82-83	32.59041018249059	35.0	34.0	36.5	26.0	39.0
84-85	32.359218537721596	35.0	34.0	36.0	26.0	37.0
86-87	32.117655537844925	35.0	33.0	36.0	25.5	37.0
88-89	31.965929630546718	35.0	33.0	35.5	26.0	37.0
90-91	31.782913032876174	35.0	33.0	35.0	25.0	36.0
92-93	31.59146230665159	35.0	33.0	35.0	25.0	36.0
94-95	31.43960920295593	35.0	33.0	35.0	24.5	36.0
96-97	31.280531042605617	35.0	33.0	35.0	24.0	36.0
98-99	31.118064901232138	35.0	33.0	35.0	23.0	35.5
100	31.01194309208699	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19309037960713482
1101	2	0.23932898707283812
1101	3	0.23517877789549857
1101	4	0.16851588622216696
1101	5	0.18843071440385017
1101	6	0.21920945236806944
1101	7	0.20468377533156001
1101	8	0.25795182570762165
1101	9	0.3264639172473096
1101	10-11	0.31012430985559547
1101	12-13	0.30817739614612805
1101	14-15	0.4378675166450847
1101	16-17	0.4247791896003221
1101	18-19	0.3606702469886329
1101	20-21	0.3252908323199719
1101	22-23	0.3826315023560056
1101	24-25	0.37391336436440525
1101	26-27	0.3227102693835491
1101	28-29	0.3971225840985966
1101	30-31	0.4335693262216722
1101	32-33	0.42483765208097424
1101	34-35	0.4539481901458515
1101	36-37	0.4428766030589202
1101	38-39	0.35334044634617356
1101	40-41	0.4111201902395365
1101	42-43	0.36417058925971446
1101	44-45	0.47803172944510663
1101	46-47	0.47484955382990535
1101	48-49	0.39738412147380586
1101	50-51	0.3001247182052751
1101	52-53	0.37644809888551123
1101	54-55	0.32319574518399463
1101	56-57	0.4085592733012504
1101	58-59	0.41481882417154736
1101	60-61	0.45569981113158775
1101	62-63	0.46081544703274346
1101	64-65	0.47314254660613386
1101	66-67	0.42860875378151064
1101	68-69	0.38321470001598357
1101	70-71	0.40873694382161574
1101	72-73	0.3945366817536282
1101	74-75	0.3325116757841897
1101	76-77	0.4214930860299617
1101	78-79	0.38672143217821286
1101	80-81	0.3992410066924421
1101	82-83	0.35121939232869437
1101	84-85	0.3438439838109346
1101	86-87	0.35176635821999014
1101	88-89	0.35763726954560227
1101	90-91	0.4037650875353478
1101	92-93	0.4044441372499037
1101	94-95	0.43758083537035475
1101	96-97	0.4422029021226095
1101	98-99	0.4947142442139736
1101	100	0.5081041991010338
1102	1	0.13305928378544962
1102	2	0.19166809049787759
1102	3	0.22219219426919068
1102	4	0.1709346886148424
1102	5	0.13510711305460887
1102	6	0.1489413907652164
1102	7	0.16428357864245413
1102	8	0.20403887711446345
1102	9	0.29726889966867276
1102	10-11	0.2731055552919983
1102	12-13	0.3257342596389847
1102	14-15	0.44142580794796515
1102	16-17	0.42858929668400236
1102	18-19	0.38974037792591076
1102	20-21	0.3313607558225371
1102	22-23	0.3023877935616781
1102	24-25	0.29867173337914465
1102	26-27	0.3197268028170299
1102	28-29	0.30724796024712475
1102	30-31	0.29539118050806223
1102	32-33	0.33364350522965225
1102	34-35	0.3783693738835723
1102	36-37	0.3075924174915343
1102	38-39	0.30690297150524515
1102	40-41	0.3889375705550364
1102	42-43	0.4220176314940147
1102	44-45	0.39353434529663645
1102	46-47	0.3918509997800683
1102	48-49	0.3913793675075823
1102	50-51	0.34015634784594795
1102	52-53	0.356246581177885
1102	54-55	0.3737656610012223
1102	56-57	0.45232799937372903
1102	58-59	0.4339093960142293
1102	60-61	0.39637865648733595
1102	62-63	0.39180106383047075
1102	64-65	0.396556346055533
1102	66-67	0.3915131112815473
1102	68-69	0.3788144494603145
1102	70-71	0.36563502413652316
1102	72-73	0.32819019916380654
1102	74-75	0.376096525101147
1102	76-77	0.3742013969477398
1102	78-79	0.3965754988057384
1102	80-81	0.39950154636336066
1102	82-83	0.35822377601424904
1102	84-85	0.37721835058460584
1102	86-87	0.3852864347298919
1102	88-89	0.39361931785684234
1102	90-91	0.405871907000261
1102	92-93	0.3881415926019329
1102	94-95	0.481504929771706
1102	96-97	0.43915933764634474
1102	98-99	0.5204499970282228
1102	100	0.545356144632386
1103	1	0.1177601826745942
1103	2	0.17763701982082836
1103	3	0.18535560420362174
1103	4	0.1821611881389984
1103	5	0.10900164651983602
1103	6	0.10114416257060554
1103	7	0.12882268466079694
1103	8	0.15233069669446309
1103	9	0.25324826796603617
1103	10-11	0.2502233354442396
1103	12-13	0.25024279243514513
1103	14-15	0.3076770392208559
1103	16-17	0.30274820280099135
1103	18-19	0.2985259201223798
1103	20-21	0.3074882114988071
1103	22-23	0.2686341679770692
1103	24-25	0.2745282562091731
1103	26-27	0.2984647732930341
1103	28-29	0.256527019519595
1103	30-31	0.299900983094993
1103	32-33	0.32279094349635074
1103	34-35	0.297742253493098
1103	36-37	0.29253127222072806
1103	38-39	0.3199052082079916
1103	40-41	0.3537755317398066
1103	42-43	0.4613665597714842
1103	44-45	0.39348678262934556
1103	46-47	0.4441248607564603
1103	48-49	0.421265864910616
1103	50-51	0.43636637781160204
1103	52-53	0.48353683593439456
1103	54-55	0.4789774496623025
1103	56-57	0.4958030602906547
1103	58-59	0.49396546161899835
1103	60-61	0.5168265009965438
1103	62-63	0.48909997207529443
1103	64-65	0.4504537755289135
1103	66-67	0.4590771207905888
1103	68-69	0.4458041527547749
1103	70-71	0.5184531951610083
1103	72-73	0.4797117971245797
1103	74-75	0.5073754468325049
1103	76-77	0.46986039907862676
1103	78-79	0.49810906036164226
1103	80-81	0.47101813590987973
1103	82-83	0.4269402623144032
1103	84-85	0.4901928041177186
1103	86-87	0.4454018572692817
1103	88-89	0.4734476805030532
1103	90-91	0.4940223952125109
1103	92-93	0.5313498499235756
1103	94-95	0.5455778277658645
1103	96-97	0.519980874840229
1103	98-99	0.5212365413769646
1103	100	0.5332283015475419
1104	1	0.15251215834230436
1104	2	0.21570397679855802
1104	3	0.16082571573878113
1104	4	0.18158868598438715
1104	5	0.12512096819910568
1104	6	0.17451061781780197
1104	7	0.14399208585047063
1104	8	0.15621165952256888
1104	9	0.27006466515442185
1104	10-11	0.261599826729622
1104	12-13	0.2687774260093576
1104	14-15	0.3897077578097594
1104	16-17	0.3273909209863959
1104	18-19	0.31791214558266745
1104	20-21	0.3026392909974831
1104	22-23	0.31442067458384315
1104	24-25	0.29714660061209486
1104	26-27	0.3383825367121176
1104	28-29	0.3537696045380301
1104	30-31	0.3603282976001978
1104	32-33	0.3316446297070641
1104	34-35	0.377361690978546
1104	36-37	0.34417614090232007
1104	38-39	0.37815963413464004
1104	40-41	0.3598031383954847
1104	42-43	0.3569884924871758
1104	44-45	0.33043964830554273
1104	46-47	0.3360741341620965
1104	48-49	0.3239222586875101
1104	50-51	0.31064143347504825
1104	52-53	0.30125671597479453
1104	54-55	0.30419790273202807
1104	56-57	0.3010091413584206
1104	58-59	0.32261353918368485
1104	60-61	0.3166445492847103
1104	62-63	0.28888121607421624
1104	64-65	0.3062543675216034
1104	66-67	0.3493900550586986
1104	68-69	0.33705176836318884
1104	70-71	0.3246201779893383
1104	72-73	0.36355677090521255
1104	74-75	0.3169234364707947
1104	76-77	0.3655006852774676
1104	78-79	0.332009648521435
1104	80-81	0.29616669166269816
1104	82-83	0.29333366078305545
1104	84-85	0.30075424179025845
1104	86-87	0.31913296694877147
1104	88-89	0.26241172378302124
1104	90-91	0.32815695581398785
1104	92-93	0.32340397743540095
1104	94-95	0.35883425100240984
1104	96-97	0.3499719440012683
1104	98-99	0.38676062707545356
1104	100	0.4269428831976967
1105	1	0.13135098525818734
1105	2	0.18558535002876653
1105	3	0.163312695468818
1105	4	0.11420990238597994
1105	5	0.13074689556724906
1105	6	0.14886838488921939
1105	7	0.2023820262868199
1105	8	0.20679367980017105
1105	9	0.22957478571546375
1105	10-11	0.20374414366492744
1105	12-13	0.16056432709602575
1105	14-15	0.2109741704761774
1105	16-17	0.2118513486128748
1105	18-19	0.2665784746003723
1105	20-21	0.25159979031072766
1105	22-23	0.22705167262496673
1105	24-25	0.25601472154472305
1105	26-27	0.29964940749520963
1105	28-29	0.3156214224553082
1105	30-31	0.32014658646652094
1105	32-33	0.2597245524041156
1105	34-35	0.25680913439052944
1105	36-37	0.2835936031225401
1105	38-39	0.28408494923832706
1105	40-41	0.29493763381812954
1105	42-43	0.35023551324410107
1105	44-45	0.32991875267046566
1105	46-47	0.3150843306517359
1105	48-49	0.27167150597634304
1105	50-51	0.2602505072394692
1105	52-53	0.26965536062810713
1105	54-55	0.2902319187440483
1105	56-57	0.3553814409090421
1105	58-59	0.3246725191614388
1105	60-61	0.2610232455015975
1105	62-63	0.23665909565571042
1105	64-65	0.334030872753047
1105	66-67	0.333291071314207
1105	68-69	0.2923417010794225
1105	70-71	0.3309750623191192
1105	72-73	0.328170226470867
1105	74-75	0.3048382759730046
1105	76-77	0.339329497253658
1105	78-79	0.2766534585591103
1105	80-81	0.2879271662037084
1105	82-83	0.25250698074616906
1105	84-85	0.2698869335905627
1105	86-87	0.24417065552953687
1105	88-89	0.2258355606120368
1105	90-91	0.24596318772792358
1105	92-93	0.2846432763811393
1105	94-95	0.27388308708910714
1105	96-97	0.24491263910001138
1105	98-99	0.3238942927978883
1105	100	0.35915985404535533
1106	1	0.11572863085275031
1106	2	0.16878378478556044
1106	3	0.13775987842487325
1106	4	0.16321546265192666
1106	5	0.12084730248187014
1106	6	0.16164059777138107
1106	7	0.1583615714234199
1106	8	0.1892284319663915
1106	9	0.17410656952210957
1106	10-11	0.2051804459487272
1106	12-13	0.18725088063849427
1106	14-15	0.29608487125219085
1106	16-17	0.25799324384139055
1106	18-19	0.2574459906716555
1106	20-21	0.23035470747112896
1106	22-23	0.23149896705864137
1106	24-25	0.2891280891333565
1106	26-27	0.2804133431948017
1106	28-29	0.24650236853355523
1106	30-31	0.26582915584161526
1106	32-33	0.2712106455866703
1106	34-35	0.29565287027593
1106	36-37	0.36073704280339314
1106	38-39	0.27491454464493614
1106	40-41	0.27396628792114797
1106	42-43	0.3157230178063912
1106	44-45	0.2675998714354577
1106	46-47	0.24303362100685177
1106	48-49	0.24614049712188546
1106	50-51	0.2858338816382897
1106	52-53	0.25678492713277734
1106	54-55	0.2593481187169928
1106	56-57	0.30495281251897666
1106	58-59	0.28757257037108985
1106	60-61	0.2990770284564448
1106	62-63	0.2497272496287053
1106	64-65	0.2927823804040486
1106	66-67	0.3022592969344231
1106	68-69	0.27413800022357293
1106	70-71	0.26535715178916064
1106	72-73	0.34254215838070223
1106	74-75	0.3206522820268489
1106	76-77	0.35062338292264883
1106	78-79	0.3565899912452153
1106	80-81	0.32563266536713087
1106	82-83	0.3321062631836327
1106	84-85	0.3173330103292571
1106	86-87	0.3572531234112688
1106	88-89	0.3219946069460029
1106	90-91	0.3617786847187432
1106	92-93	0.3589777314046856
1106	94-95	0.42586328592314615
1106	96-97	0.42927024004443837
1106	98-99	0.43107878761377094
1106	100	0.4442922792503481
1107	1	0.06325159200530095
1107	2	0.10734616416730347
1107	3	0.07122786263494874
1107	4	0.13463181511111344
1107	5	0.11753129902797355
1107	6	0.10298552075823864
1107	7	0.03150519495247295
1107	8	0.0518434465855222
1107	9	0.107998358438266
1107	10-11	0.0722416025115109
1107	12-13	-0.0037347687178694855
1107	14-15	0.11164081062703701
1107	16-17	0.11328725469663681
1107	18-19	0.08109227198497138
1107	20-21	0.09925237327303904
1107	22-23	0.09903549552143431
1107	24-25	0.15455502772012863
1107	26-27	0.11770071834655482
1107	28-29	0.14746026446310623
1107	30-31	0.129007424688254
1107	32-33	0.10436582874321942
1107	34-35	0.14400899019731384
1107	36-37	0.14809829544601882
1107	38-39	0.13821597591218193
1107	40-41	0.11753671348557049
1107	42-43	0.11900775416687281
1107	44-45	0.08753142214030873
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2216	72-73	-0.9701864099914062
2216	74-75	-0.9774346671728011
2216	76-77	-0.9143620652791213
2216	78-79	-0.775511407281364
2216	80-81	-0.8211247157746797
2216	82-83	-0.8987909550342366
2216	84-85	-0.9185388265157357
2216	86-87	-0.9161064672398389
2216	88-89	-0.8975677300579541
2216	90-91	-0.9012613158716078
2216	92-93	-0.8425338183434192
2216	94-95	-0.9325126000702575
2216	96-97	-1.102438542339339
2216	98-99	-1.195524196071915
2216	100	-1.220976318200151
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	274.0
3	719.0
4	479.0
5	354.0
6	553.0
7	1172.0
8	2220.0
9	3562.0
10	5207.0
11	7667.0
12	10660.0
13	15304.0
14	18087.0
15	24757.0
16	32260.0
17	47058.0
18	51279.0
19	27383.0
20	17917.0
21	16051.0
22	16258.0
23	16946.0
24	18965.0
25	23508.0
26	29441.0
27	37906.0
28	48630.0
29	62276.0
30	79424.0
31	100788.0
32	128292.0
33	161942.0
34	218998.0
35	326364.0
36	518136.0
37	978830.0
38	1490165.0
39	766215.0
40	4638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.3477268137413	21.58017943748323	15.211964860033941	23.860128888741528
2	14.173802437915258	21.72879769747217	39.644151366537464	24.453248498075105
3	16.345925894542596	27.17208264486153	31.84625306575906	24.635738394836814
4	11.306784017649377	18.576185963671268	38.65838416153674	31.458645857142614
5	11.667614170795167	38.7401954978144	35.43427544557017	14.157914885820261
6	28.34105204884116	39.556535550498666	17.794088709836867	14.308323690823313
7	24.855381179640666	34.63846942531896	22.627175213743612	17.878974181296766
8	25.19083696359321	35.30380732429056	20.335587593690832	19.169768118425402
9	25.343058381357586	16.198531827815053	22.105698229136166	36.352711561691194
10-11	23.24312440151292	27.505322239097296	28.803741432866342	20.44781192652344
12-13	24.590387115786385	25.587838480346697	29.14320845615961	20.678565947707312
14-15	21.826534618415515	26.971708922671933	27.89556337835891	23.30619308055364
16-17	19.62749626460617	30.19714970855925	28.706786412675577	21.468567614159
18-19	19.480914420644417	27.76823517116907	32.74025855421664	20.010591853969874
20-21	20.9707128176497	26.787608378203565	32.657171344186295	19.584507459960438
22-23	20.549985548003693	26.808603786517182	32.76871062772505	19.872700037754075
24-25	20.252605375419794	26.858749062027186	32.76559106174285	20.123054500810163
26-27	19.957547849288744	26.9752489556432	32.78906581730982	20.278137377758238
28-29	19.970877500308106	26.974296371804652	32.99965606979383	20.05517005809342
30-31	20.35613124674465	27.191663813820433	32.545608819983705	19.906596119451216
32-33	19.91919489014493	27.106609154874544	32.86067563111986	20.11352032386066
34-35	20.320909487714555	26.961193041682343	32.55620746003178	20.161690010571323
36-37	20.419815829912654	27.085956491810563	32.18592737010263	20.308300308174143
38-39	20.32928596553467	27.229328747797805	32.23842186173646	20.20296342493106
40-41	20.311884047600778	27.04411107726912	32.216051681274955	20.427953193855153
42-43	20.263133068440887	27.10254222558992	32.14893732006448	20.485387385904712
44-45	20.567591563046953	27.193271677759085	31.69945120073964	20.539685558454323
46-47	20.690194750728274	27.40976381374784	31.145523235763235	20.754518199760653
48-49	20.70389556100372	27.512503154033585	30.656236701276303	21.127364583686397
50-51	20.795083193495127	27.61323740822956	30.353520218001673	21.238159180273648
52-53	20.84952997683425	27.76165972384697	30.21031824303745	21.178492056281325
54-55	20.930863806746718	28.040411051313814	30.07832503377897	20.950400108160498
56-57	20.84180301404979	27.69623962653404	30.133803458087193	21.328153901328978
58-59	20.837272296540466	27.301176830384417	30.424394881092905	21.43715599198222
60-61	20.79715785106078	27.67718026648253	30.25841851705151	21.267243365405175
62-63	20.62274093762328	27.49502641437665	30.43084950631337	21.451383141686698
64-65	20.642050545646647	27.41188396734057	30.48381024043213	21.462255246580654
66-67	20.646024797019816	27.39049257077038	30.29620414792573	21.66727848428407
68-69	21.002203120145367	27.526686940393358	30.078681517328388	21.392428422132884
70-71	20.920513634308165	27.434894929835064	30.004337497814536	21.64025393804223
72-73	20.80213757735584	27.419146494284636	30.139755982514497	21.638959945845027
74-75	20.86209928830153	27.503532506972743	29.910646026984132	21.723722177741593
76-77	20.917772377762944	27.412527762426127	29.740683169873567	21.92901668993736
78-79	20.907099798791126	27.463274037709695	29.92493104187519	21.704695121623992
80-81	20.64591479253618	27.47149379034908	29.921582620697706	21.961008796417033
82-83	20.629449274958425	27.430633654993436	30.063693991659928	21.87622307838821
84-85	20.60327055886923	27.287854673964702	29.91166621348942	22.19720855367665
86-87	20.713335473672416	27.39691772341986	29.698362828900603	22.19138397400712
88-89	20.558054186983412	27.27734746082662	29.763015505532714	22.40158284665726
90-91	20.405211380452805	27.26028751469914	29.675616234063966	22.65888487078409
92-93	20.403824486668213	27.35372453230273	29.674475374921844	22.56797560610721
94-95	20.208617155790638	27.087946281882658	29.8606676916443	22.842768870682406
96-97	20.280942323020685	27.27119947170438	29.614805157096203	22.83305304817873
98-99	20.136964298559086	27.117742937122248	29.729313941371437	23.01597882294723
100	20.202727754262224	27.11377794398408	29.58958654738226	23.093907754371436
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2281.0
1	1377.5
2	1969.0
3	3711.5
4	2587.5
5	1519.5
6	3866.0
7	10298.0
8	16897.0
9	18522.5
10	12966.0
11	6760.5
12	5534.5
13	5826.0
14	6438.5
15	7038.5
16	7581.0
17	8342.5
18	9242.5
19	10391.0
20	11863.5
21	13764.5
22	16864.5
23	21597.5
24	27552.5
25	34801.0
26	45364.0
27	59182.5
28	76463.5
29	93280.0
30	105236.0
31	117287.5
32	130203.0
33	142077.0
34	153354.5
35	163043.5
36	169690.0
37	174885.0
38	178942.5
39	180559.0
40	182626.0
41	183649.5
42	180725.5
43	179491.0
44	178569.0
45	174684.5
46	170886.5
47	167217.0
48	163543.0
49	161682.5
50	161474.5
51	154150.5
52	141992.0
53	131732.5
54	123771.5
55	117312.0
56	105805.0
57	92345.5
58	83150.0
59	76515.5
60	65568.0
61	52061.0
62	43428.0
63	36131.0
64	28629.5
65	23012.5
66	18396.5
67	14101.0
68	10661.5
69	7943.0
70	5683.0
71	4127.5
72	3180.0
73	2495.0
74	1916.0
75	1413.0
76	878.0
77	620.5
78	487.0
79	290.5
80	152.0
81	63.5
82	39.0
83	20.5
84	10.5
85	5.5
86	5.5
87	6.0
88	2.0
89	0.0
90	0.5
91	2.0
92	2.0
93	0.5
94	1.5
95	3.0
96	2.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04784693499991056
2	0.0
3	1.8829962613109232E-4
4	0.07072533957483827
5	0.004556850952372434
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.007908584297505877
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.648988783932769E-4
26-27	0.0
28-29	2.730344578900839E-4
30-31	0.0037471625600087374
32-33	1.1297977567865538E-4
34-35	0.00171352659779294
36-37	0.004020197017898821
38-39	0.001374587270756974
40-41	0.001082722850253781
42-43	0.0019206561865371418
44-45	0.0
46-47	4.613340840211762E-4
48-49	4.7074906532773076E-4
50-51	0.0015723018781946209
52-53	8.002734110571423E-4
54-55	0.0011015528128668901
56-57	0.006053832980114618
58-59	0.009207851717810415
60-61	0.0010450629250275624
62-63	0.0024478951397042003
64-65	0.0011203827754799993
66-67	1.2239475698521E-4
68-69	6.590486914588231E-4
70-71	3.6718427095563E-4
72-73	0.0
74-75	1.6946966351798307E-4
76-77	0.0
78-79	6.496337101522685E-4
80-81	0.0
82-83	0.0011015528128668901
84-85	6.213887662326047E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.1654457005075616E-4
94-95	0.0
96-97	4.1425917748840314E-4
98-99	5.837288410063862E-4
100	3.0127940180974775E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5310685.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.73308846412116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.06060329692126	35.72718299745205
2	17.531734103087	18.13941502568899
3	6.221418463447413	9.655595752255252
4	2.7051434176734346	5.597816949385393
5	1.3987653197097378	3.6181225012544282
6	0.8201395169419647	2.5457010109728153
7	0.5021025643147847	1.8182721464431186
8	0.3340085521182398	1.382343517960474
9	0.2503175239764092	1.1654728750792211
>10	1.062458111078672	9.553250945113762
>50	0.06793530918144565	2.414597973314069
>100	0.04074608436338731	4.122014055490983
>500	0.0029879919085844175	1.051544589245982
>1k	0.0014211125739605312	1.1497546011528743
>5k	1.0931635184007463E-4	0.3737004068209853
>10k+	1.0931635184007463E-4	1.6852146523696339
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35643	0.6711563574190523	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31919	0.6010335766478336	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21835	0.41115223365724	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7973	0.15013129191431993	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6556	0.12344923489154412	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.882996261310923E-5	0.0
5	0.0	0.0	0.0	1.882996261310923E-5	0.0
6	0.0	0.0	0.0	1.882996261310923E-5	0.0
7	0.0	0.0	0.0	3.765992522621846E-5	0.0
8	0.0	0.0	0.0	7.531985045243692E-5	0.0
9	0.0	0.0	0.0	9.414981306554616E-5	0.0
10-11	0.0	0.0	0.0	1.5063970090487385E-4	0.0
12-13	0.0	0.0	0.0	1.9771460743764693E-4	0.0
14-15	0.0	0.0	0.0	2.8244943919663847E-4	0.0
16-17	0.0	0.0	0.0	3.012794018097477E-4	0.0
18-19	0.0	0.0	0.0	3.2010936442285697E-4	0.0
20-21	0.0	0.0	0.0	3.7659925226218464E-4	0.0
22-23	0.0	1.882996261310923E-5	0.0	6.590486914588231E-4	0.0
24-25	0.0	1.882996261310923E-5	0.0	0.0012616074950783185	0.0
26-27	0.0	1.882996261310923E-5	0.0	0.0022595955135731077	0.0
28-29	0.0	1.882996261310923E-5	0.0	0.005366539344736131	0.0
30-31	0.0	1.882996261310923E-5	0.0	0.013679967838423856	0.0
32-33	0.0	1.882996261310923E-5	0.0	0.03065517913414183	0.0
34-35	0.0	1.882996261310923E-5	0.0	0.05698888184857509	0.0
36-37	0.0	1.882996261310923E-5	0.0	0.09995885653169036	0.0
38-39	0.0	1.882996261310923E-5	0.0	0.15819051591273064	0.0
40-41	0.0	1.882996261310923E-5	0.0	0.21806979702241802	0.0
42-43	0.0	1.882996261310923E-5	0.0	0.2801710137204523	0.0
44-45	0.0	1.882996261310923E-5	0.0	0.3617329967791349	0.0
46-47	0.0	1.882996261310923E-5	0.0	0.46424331324490153	0.0
48-49	0.0	1.882996261310923E-5	0.0	0.5728827825412353	0.0
50-51	0.0	1.882996261310923E-5	0.0	0.6851093597153664	0.0
52-53	0.0	1.882996261310923E-5	0.0	0.7955282604786389	0.0
54-55	0.0	1.882996261310923E-5	0.0	0.9223951335844622	0.0
56-57	0.0	1.882996261310923E-5	0.0	1.076160608283112	0.0
58-59	0.0	1.882996261310923E-5	0.0	1.2477863025202964	0.0
60-61	0.0	1.882996261310923E-5	0.0	1.4296178364937857	0.0
62-63	0.0	1.882996261310923E-5	0.0	1.612758052868886	0.0
64-65	0.0	1.882996261310923E-5	0.0	1.8088815284657254	0.0
66-67	0.0	1.882996261310923E-5	0.0	2.0461861322974344	0.0
68-69	0.0	1.882996261310923E-5	0.0	2.316198381188114	0.0
70-71	0.0	1.882996261310923E-5	0.0	2.610811976232821	0.0
72-73	0.0	1.882996261310923E-5	0.0	2.9144827832944338	0.0
74-75	0.0	1.882996261310923E-5	0.0	3.2247252473080215	0.0
76-77	0.0	1.882996261310923E-5	0.0	3.561442638755641	0.0
78-79	0.0	1.882996261310923E-5	0.0	3.9532753307718305	0.0
80-81	0.0	1.882996261310923E-5	0.0	4.38489573378952	0.0
82-83	0.0	1.882996261310923E-5	0.0	4.834169226757	0.0
84-85	0.0	1.882996261310923E-5	0.0	5.273302784857321	0.0
86-87	0.0	1.882996261310923E-5	0.0	5.7438823805215335	0.0
88	0.0	1.882996261310923E-5	0.0	6.124464169876391	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14220	0.0	62.198498	1
GTATCAA	27205	0.0	43.578617	1
GTGGTAT	4935	0.0	37.17912	1
TGGTATC	4950	0.0	37.049694	2
ATCAACG	35985	0.0	32.643456	3
TATCAAC	36425	0.0	32.36533	2
TCAACGC	36430	0.0	32.309254	4
CAACGCA	38255	0.0	30.718288	5
AACGCAG	39645	0.0	29.676003	6
ACGCAGA	44465	0.0	26.321707	7
CGCAGAG	44240	0.0	26.30683	8
GCAGAGT	46885	0.0	24.511953	9
CATGGGT	11350	0.0	24.320217	4
GTACATG	24025	0.0	23.63554	1
TACATGG	23825	0.0	23.448158	2
ACATGGG	24405	0.0	22.27431	3
GAGTACT	33385	0.0	22.185452	12-13
CAGAGTA	46725	0.0	21.240988	10-11
AGAGTAC	45060	0.0	21.212214	10-11
AGTACTT	33895	0.0	20.908802	12-13
>>END_MODULE