FastQCFastQC Report
Wed 25 May 2016
SRR2431445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4329276
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA443591.0246285984076782No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63750.1472532589744798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65000.044.551691
GTATCAA103000.032.314161
TACCTGG36200.030.775242
TCAACGC116450.027.0453114
ATCAACG117600.026.901063
GTACATG201950.026.1881891
TATCAAC126550.025.6300012
TACATGG203050.024.9329912
GTACCTG46350.024.5449681
CATGGGT97400.024.5166664
CAACGCA130100.024.2438665
ACATGGG207700.023.288453
AACGCAG137750.022.928956
TCTGTCG12400.021.98388
GTGGTAT36600.021.5786881
CGCAGAG147750.020.8358068
ACGCAGA150400.020.6879127
GTCTCGC11650.020.5797961
ATGGGTG58650.020.2772565
TATAACG3303.274181E-1119.9423262
Produced by FastQC (version 0.11.5)